Click here to switch to the map view.

The map label for this gene is prpB [C]

Identifier: 66048336

GI number: 66048336

Start: 6063869

End: 6064690

Strand: Direct

Name: prpB [C]

Synonym: Psyr_5112

Alternate gene names: 66048336

Gene position: 6063869-6064690 (Clockwise)

Preceding gene: 66048335

Following gene: 66048338

Centisome position: 99.51

GC content: 56.45

Gene sequence:

>822_bases
ATGAAATCACTGGACGATATTTTTCATGAGTTACACAACCATGGTCTTTTGATCCTGGCCAATGTCAGCGATGCAGGCGG
GGCAAAAGTCTTAGAGTCATTGGGTGCCACCGCGATCGCCACCAGCAGTGCAGGCATGGCGTGGGCGCACGGTTACGCCG
ATGGCAACCAGCTCCCCCTTACGCGGCTGGCAGCCAGCGTTGAATCTATGACGCGAATACTGCGCGTGCCCCTGACCGTA
GACATTGAGGCCGGTTACTCCGAGGATCTTCAACATGTCGCCAGCGTTGTCGAAGCGGTTGTCGCCGCAGGTGCCGTGGG
TATCAACATCGAAGACGGTACCGCACCACCGGAAGTACTTGTGCAGAAAATCGAAGTTGCCCGAGAAGTCGCCGACAAGC
ATGGTATTAATCTATTCATCAATGCGCGCAGTGATGTCTATCTGAAAAGCCTGGTGCCACCCGTCCAGCGTCTGGATGAA
ATCATCCGGCGTGCAGCGCTTTATGCTCAAGCAGGAGCCAACGGTCTGTTCGCCGCCGGTATGGTCACACCGGCCGAGAT
TTCCACACTGTGCAACAACTGCACGCTACCGGTGAACCTGCTTGCACGCGATGGGCTGTCGGCACCCGGCGATCTGGAGC
GCTTAGGCGTTAGACGCCTGAGCGCCGGTTCCAGTATTGCGGAGTTTCTGTATGGAGCGATGGAAGGGCTGGCGCGCTCT
TTCATCGAGCGCGGGCAGCTGGATACGCAAGCGCTTACGGGTTTTACGTATGCAAAGTTGAATGGGTTAATAGGGAGCGT
GGATAAAGCAACAGGCCCATAG

Upstream 100 bases:

>100_bases
GACGCCCCAACGCAGCTACTCCATACGAAGGCAGGCTTTCTATCTCCAGTCGCTTGCCTGCTACCGTCAATAGATGGCAA
CTTTCAGACAGGAAAGTGTT

Downstream 100 bases:

>100_bases
GCAGAGTGCGCGGTAGATGCCCAGGGGCCACAAGCCCAAGCGTGCTTGGGAAATCCCTGTTGGTTTGGCAAGGTCCCTAA
AAACACTATCAGGTCATGAA

Product: hypothetical protein

Products: Pyruvate; Succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MKSLDDIFHELHNHGLLILANVSDAGGAKVLESLGATAIATSSAGMAWAHGYADGNQLPLTRLAASVESMTRILRVPLTV
DIEAGYSEDLQHVASVVEAVVAAGAVGINIEDGTAPPEVLVQKIEVAREVADKHGINLFINARSDVYLKSLVPPVQRLDE
IIRRAALYAQAGANGLFAAGMVTPAEISTLCNNCTLPVNLLARDGLSAPGDLERLGVRRLSAGSSIAEFLYGAMEGLARS
FIERGQLDTQALTGFTYAKLNGLIGSVDKATGP

Sequences:

>Translated_273_residues
MKSLDDIFHELHNHGLLILANVSDAGGAKVLESLGATAIATSSAGMAWAHGYADGNQLPLTRLAASVESMTRILRVPLTV
DIEAGYSEDLQHVASVVEAVVAAGAVGINIEDGTAPPEVLVQKIEVAREVADKHGINLFINARSDVYLKSLVPPVQRLDE
IIRRAALYAQAGANGLFAAGMVTPAEISTLCNNCTLPVNLLARDGLSAPGDLERLGVRRLSAGSSIAEFLYGAMEGLARS
FIERGQLDTQALTGFTYAKLNGLIGSVDKATGP
>Mature_273_residues
MKSLDDIFHELHNHGLLILANVSDAGGAKVLESLGATAIATSSAGMAWAHGYADGNQLPLTRLAASVESMTRILRVPLTV
DIEAGYSEDLQHVASVVEAVVAAGAVGINIEDGTAPPEVLVQKIEVAREVADKHGINLFINARSDVYLKSLVPPVQRLDE
IIRRAALYAQAGANGLFAAGMVTPAEISTLCNNCTLPVNLLARDGLSAPGDLERLGVRRLSAGSSIAEFLYGAMEGLARS
FIERGQLDTQALTGFTYAKLNGLIGSVDKATGP

Specific function: Catalyzes The Formation Of Pyruvate And Succinate From 2-Methylisocitrate (By Similarity). [C]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR015813 [H]

Pfam domain/function: NA

EC number: 4.1.3.30 [C]

Molecular weight: Translated: 28612; Mature: 28612

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLDDIFHELHNHGLLILANVSDAGGAKVLESLGATAIATSSAGMAWAHGYADGNQLPL
CCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCHHEEECCCCCCEECCCCCCCCCHH
TRLAASVESMTRILRVPLTVDIEAGYSEDLQHVASVVEAVVAAGAVGINIEDGTAPPEVL
HHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHH
VQKIEVAREVADKHGINLFINARSDVYLKSLVPPVQRLDEIIRRAALYAQAGANGLFAAG
HHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEECC
MVTPAEISTLCNNCTLPVNLLARDGLSAPGDLERLGVRRLSAGSSIAEFLYGAMEGLARS
CCCHHHHHHHHCCCCCCHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
FIERGQLDTQALTGFTYAKLNGLIGSVDKATGP
HHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKSLDDIFHELHNHGLLILANVSDAGGAKVLESLGATAIATSSAGMAWAHGYADGNQLPL
CCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCHHEEECCCCCCEECCCCCCCCCHH
TRLAASVESMTRILRVPLTVDIEAGYSEDLQHVASVVEAVVAAGAVGINIEDGTAPPEVL
HHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHH
VQKIEVAREVADKHGINLFINARSDVYLKSLVPPVQRLDEIIRRAALYAQAGANGLFAAG
HHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEECC
MVTPAEISTLCNNCTLPVNLLARDGLSAPGDLERLGVRRLSAGSSIAEFLYGAMEGLARS
CCCHHHHHHHHCCCCCCHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
FIERGQLDTQALTGFTYAKLNGLIGSVDKATGP
HHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: methylisocitrate [C]

Specific reaction: methylisocitrate <==> Pyruvate + Succinate [C]

General reaction: Elimination [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]