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The map label for this gene is hemK [H]

Identifier: 66048324

GI number: 66048324

Start: 6052034

End: 6052981

Strand: Direct

Name: hemK [H]

Synonym: Psyr_5100

Alternate gene names: 66048324

Gene position: 6052034-6052981 (Clockwise)

Preceding gene: 66048323

Following gene: 66048325

Centisome position: 99.32

GC content: 61.39

Gene sequence:

>948_bases
ATGAGTCATCCCCTCCCCGACAGCGCCGCGCTGATTGCCCTGGCGCAGCGTCTTGAAACCAGCGGCTATCGCTTTATCAC
GCCCACGCCCCTGACTCATCAACACGTCAATCAACGCCCGGAAAATCGTACTGCCGCGTCACTGCGCGATGTGTTCGGCT
GGAGTCGGCTGATACCGGAGTCCATGCTGCCGGTCGAGGAGGCTCAAGGGTTGCTGGCTGCCGGTATTCTGGAACGTAAG
GAAGACGGCTTGAAAAGCCGGGTGAGATTTTCCAGCCTGGATGATCTGCTGCTGGTGCATTCGGCGTTTCCTACCTCAGA
TGAAGACTCGGTGTTTTTCGGTCCGGATACCTACCGCTTCGCGCAGTCCATCAATCGCCATCTGCAAGGTACGTCGCATC
CGGTCAACCGCGCCGTCGACATCGGCTGTGGCACGGGTGCCGGCGCCATGTTGATCGCGGTCGCCCGTCCGCAGGCACAG
GTCCACGCCGTCGACATCAACCCCAAGGCGCTGCATTTCGCACAGACCAATGCGGCAGTCGCCGGGCTGAAGAATATGGA
GTGCTGCCACAGCGATATTCTTTCGGGCCTGAGCGGAAATTTCGACCTGATCGTCGCCAATCCGCCCTACATGAAAGACA
CCCAGCGCCGCGCCTACCGCCACGGAGGTGATGCACTGGGCGCAGACCTGTCTGTGCGCATCCTGCGCGAGTCACTGGAC
CGCCTCACTCCGGGCGGCTCGCTGGTGCTTTACACCGGCGTGGCCATGGTGGGCGAGCACGATCCGTTTTTCGAAGCGGT
GCAGGCCGATATCGACCATGCGGCACTGACATGGACCTACCGCGAACTGGACCCGGATGTCTTCGGCGAAGAGCTGCTTG
AAGAGGGTTACGAAGACGTGGACCGTATTGCAGCGGTGGAGCTGGTGGTCACCCGACGCCCCGCATGA

Upstream 100 bases:

>100_bases
TCGAAAGCATGTCGCCGGCAAGACACGCGACCGCCGCCGGCCTGCACGCGACGCGTCTGTTTGTGAACAGCATGGCCGAG
CGCATGACAGGAGCCCACGC

Downstream 100 bases:

>100_bases
CCCACCGAAATATCTTCAATAGTTCGTGCTTATAACGCGCATAAGAACCAAGCATTGGTCAGCCTGACCGCATCCCGGTG
AAACTTGCCACGAGCCATCG

Product: hypothetical protein

Products: NA

Alternate protein names: M.EcoKHemKP; Protein-(glutamine-N(5)) MTase hemK; Protein-glutamine N-methyltransferase hemK [H]

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MSHPLPDSAALIALAQRLETSGYRFITPTPLTHQHVNQRPENRTAASLRDVFGWSRLIPESMLPVEEAQGLLAAGILERK
EDGLKSRVRFSSLDDLLLVHSAFPTSDEDSVFFGPDTYRFAQSINRHLQGTSHPVNRAVDIGCGTGAGAMLIAVARPQAQ
VHAVDINPKALHFAQTNAAVAGLKNMECCHSDILSGLSGNFDLIVANPPYMKDTQRRAYRHGGDALGADLSVRILRESLD
RLTPGGSLVLYTGVAMVGEHDPFFEAVQADIDHAALTWTYRELDPDVFGEELLEEGYEDVDRIAAVELVVTRRPA

Sequences:

>Translated_315_residues
MSHPLPDSAALIALAQRLETSGYRFITPTPLTHQHVNQRPENRTAASLRDVFGWSRLIPESMLPVEEAQGLLAAGILERK
EDGLKSRVRFSSLDDLLLVHSAFPTSDEDSVFFGPDTYRFAQSINRHLQGTSHPVNRAVDIGCGTGAGAMLIAVARPQAQ
VHAVDINPKALHFAQTNAAVAGLKNMECCHSDILSGLSGNFDLIVANPPYMKDTQRRAYRHGGDALGADLSVRILRESLD
RLTPGGSLVLYTGVAMVGEHDPFFEAVQADIDHAALTWTYRELDPDVFGEELLEEGYEDVDRIAAVELVVTRRPA
>Mature_314_residues
SHPLPDSAALIALAQRLETSGYRFITPTPLTHQHVNQRPENRTAASLRDVFGWSRLIPESMLPVEEAQGLLAAGILERKE
DGLKSRVRFSSLDDLLLVHSAFPTSDEDSVFFGPDTYRFAQSINRHLQGTSHPVNRAVDIGCGTGAGAMLIAVARPQAQV
HAVDINPKALHFAQTNAAVAGLKNMECCHSDILSGLSGNFDLIVANPPYMKDTQRRAYRHGGDALGADLSVRILRESLDR
LTPGGSLVLYTGVAMVGEHDPFFEAVQADIDHAALTWTYRELDPDVFGEELLEEGYEDVDRIAAVELVVTRRPA

Specific function: Methylates the translation termination release factor RF1/PrfA on 'Gln-235' and RF2/PrfB on 'Gln-252' [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Escherichia coli, GI1787463, Length=130, Percent_Identity=37.6923076923077, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 34430; Mature: 34299

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHPLPDSAALIALAQRLETSGYRFITPTPLTHQHVNQRPENRTAASLRDVFGWSRLIPE
CCCCCCCHHHHHHHHHHHHHCCEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCH
SMLPVEEAQGLLAAGILERKEDGLKSRVRFSSLDDLLLVHSAFPTSDEDSVFFGPDTYRF
HCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCEEECCCHHHH
AQSINRHLQGTSHPVNRAVDIGCGTGAGAMLIAVARPQAQVHAVDINPKALHFAQTNAAV
HHHHHHHHCCCCCCCCCEEECCCCCCCCEEEEEEECCCCEEEEEECCCCEEEEECCCHHH
AGLKNMECCHSDILSGLSGNFDLIVANPPYMKDTQRRAYRHGGDALGADLSVRILRESLD
HCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHH
RLTPGGSLVLYTGVAMVGEHDPFFEAVQADIDHAALTWTYRELDPDVFGEELLEEGYEDV
HCCCCCCEEEEECCHHCCCCCHHHHHHHHCCCCCEEEEEHHHCCHHHHHHHHHHHHHHHH
DRIAAVELVVTRRPA
HHHHHHHHEEECCCC
>Mature Secondary Structure 
SHPLPDSAALIALAQRLETSGYRFITPTPLTHQHVNQRPENRTAASLRDVFGWSRLIPE
CCCCCCHHHHHHHHHHHHHCCEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCH
SMLPVEEAQGLLAAGILERKEDGLKSRVRFSSLDDLLLVHSAFPTSDEDSVFFGPDTYRF
HCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCEEECCCHHHH
AQSINRHLQGTSHPVNRAVDIGCGTGAGAMLIAVARPQAQVHAVDINPKALHFAQTNAAV
HHHHHHHHCCCCCCCCCEEECCCCCCCCEEEEEEECCCCEEEEEECCCCEEEEECCCHHH
AGLKNMECCHSDILSGLSGNFDLIVANPPYMKDTQRRAYRHGGDALGADLSVRILRESLD
HCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHH
RLTPGGSLVLYTGVAMVGEHDPFFEAVQADIDHAALTWTYRELDPDVFGEELLEEGYEDV
HCCCCCCEEEEECCHHCCCCCHHHHHHHHCCCCCEEEEEHHHCCHHHHHHHHHHHHHHHH
DRIAAVELVVTRRPA
HHHHHHHHEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7883187; 7543480; 11805295; 8905232; 9278503 [H]