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The map label for this gene is cls [H]

Identifier: 66048303

GI number: 66048303

Start: 6022410

End: 6023849

Strand: Direct

Name: cls [H]

Synonym: Psyr_5079

Alternate gene names: 66048303

Gene position: 6022410-6023849 (Clockwise)

Preceding gene: 66048300

Following gene: 66048309

Centisome position: 98.83

GC content: 58.75

Gene sequence:

>1440_bases
ATGGATTATCACGACCCGTACTTTTTCGGTTACGTCCTGGGTTTCATTCATTTACTCGGTACAGGTGCCGCCATCCATGC
ATTGCTGACGGTGCGGACTTCCCAAGGCGCAATTGCCTGGGCGATGCCGCTGCTGTTCATTCCCTATTTCACCTTGCTGC
CTTATCTGGTGTTCGGCCGAAGCTCATTCGACGCGTACATCAAGGCGCGCCGCCAGGCCAACCAGGAAATGCGGATCGCG
ATCGGCGACCTCAACTGGCGCCCATGGATGGAAGAAGCTGTGGCTGCGCGTCGATCAGAGGCGTATGCGGCGCTGCGTGC
AATGCCCAAGCTGGGCAACATGCCGGCGCTGGCCAACAACAAGGTCAAGTTGCTGATCAATGGCGAAGAAACCTTCGGGG
CCATTTTTCAGGCCATCCGTGAAGCGAAAAAGACCATCCTGGTTCAGTTCTTCATCATTCATGACGACAAACTTGGCCGC
GAACTGCAATCGCTGCTGCTGGAAAAAGCCGCCGAAGGCGTGGCGATCTTTGTACTTTACGACCGCATCGGCAGCCACGC
TCTGCCAGGTGCCTATATCGATAAGCTACGCGACGGTGGCGTGCAGATAAAAGCCTTCGCCACACGCGGCGGCTGGCTCA
ATCGCTTTCAGATCAACTTCCGCAACCACCGCAAGATCGTCGTCGTCGATGGCCTGAAAGGCTATATAGGCGGTCATAAC
GTGGGTGATGAATACATGGGCCTGAAGCCGCCGCTGGCCCCATGGCGCGATACTCACGTGCAAGTCATCGGCCCGGTGGT
CGCCTGCTTGCAGGAGTCGTTTGCCGAGGACTGGTTCTGGGCCACCCGCGAACTGCCCCCGCTCAGCCTGCCTGATGAAT
TTCCCGAAGACGGTGTGCTTTGCCAGTTGCTGACCAGTGGCCCGGCTGATGCTCAGGAAACCTGCTCGCTGTTCTTCGTT
GAAGCCATCCATGCTGCCGAAGAGCGCGTGTGGATCACCAGCCCCTACTTCATCCCTGACGAAGCCGTCACTGCTGCGCT
GACCCTGGCAGTGTTGCGCGGCGTGGACGTGCGCCTGCTGCTGCCGTCGCGCCCCGACCACTACGTGGTGTATGCCGCTT
CAAGTCTGTACGCCTTTGATGCTGTGCGTGCCGGAGTGCGGGTATTCCGCTACGAACCGGGCTTCCTGCATCAGAAGGTC
GTCCTGGTCGACAACGAGATTACCGCCATCGGCAGCGCCAACCTCGACAACCGCTCGTTCCGCCTCAACTTCGAATTGAT
GCTGCTGACCGTCGACAGCGACTTTTCCAGCCAGGTGGAATCCATGCTCACCGCCGACTTCAACCTCGCCCGCGAAATCT
CCGTACAGGAAAGCCATGAAACGCGGCGTCTGCATCAACTGGGTATGCGCGTGGCGCGGCTGATTTCACCGATTCTGTGA

Upstream 100 bases:

>100_bases
AGACGGCACACATGTCGCCCAGTTGCACCCGGAATTAAATAGCGACATTCCTGTTCAGCTGTGGTCTGTTAGGCAGCAAA
GGCGAGGAGCATTGTTCCAC

Downstream 100 bases:

>100_bases
GACCTGTGCTCGCTACGCTGCTTGCCTTGAGGGGCTAAACGGGACGTCACCCATAAATATCCTCACGCGTCAGTGGCAGT
CCGGTCTTGCCGTCGGCGAA

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MDYHDPYFFGYVLGFIHLLGTGAAIHALLTVRTSQGAIAWAMPLLFIPYFTLLPYLVFGRSSFDAYIKARRQANQEMRIA
IGDLNWRPWMEEAVAARRSEAYAALRAMPKLGNMPALANNKVKLLINGEETFGAIFQAIREAKKTILVQFFIIHDDKLGR
ELQSLLLEKAAEGVAIFVLYDRIGSHALPGAYIDKLRDGGVQIKAFATRGGWLNRFQINFRNHRKIVVVDGLKGYIGGHN
VGDEYMGLKPPLAPWRDTHVQVIGPVVACLQESFAEDWFWATRELPPLSLPDEFPEDGVLCQLLTSGPADAQETCSLFFV
EAIHAAEERVWITSPYFIPDEAVTAALTLAVLRGVDVRLLLPSRPDHYVVYAASSLYAFDAVRAGVRVFRYEPGFLHQKV
VLVDNEITAIGSANLDNRSFRLNFELMLLTVDSDFSSQVESMLTADFNLAREISVQESHETRRLHQLGMRVARLISPIL

Sequences:

>Translated_479_residues
MDYHDPYFFGYVLGFIHLLGTGAAIHALLTVRTSQGAIAWAMPLLFIPYFTLLPYLVFGRSSFDAYIKARRQANQEMRIA
IGDLNWRPWMEEAVAARRSEAYAALRAMPKLGNMPALANNKVKLLINGEETFGAIFQAIREAKKTILVQFFIIHDDKLGR
ELQSLLLEKAAEGVAIFVLYDRIGSHALPGAYIDKLRDGGVQIKAFATRGGWLNRFQINFRNHRKIVVVDGLKGYIGGHN
VGDEYMGLKPPLAPWRDTHVQVIGPVVACLQESFAEDWFWATRELPPLSLPDEFPEDGVLCQLLTSGPADAQETCSLFFV
EAIHAAEERVWITSPYFIPDEAVTAALTLAVLRGVDVRLLLPSRPDHYVVYAASSLYAFDAVRAGVRVFRYEPGFLHQKV
VLVDNEITAIGSANLDNRSFRLNFELMLLTVDSDFSSQVESMLTADFNLAREISVQESHETRRLHQLGMRVARLISPIL
>Mature_479_residues
MDYHDPYFFGYVLGFIHLLGTGAAIHALLTVRTSQGAIAWAMPLLFIPYFTLLPYLVFGRSSFDAYIKARRQANQEMRIA
IGDLNWRPWMEEAVAARRSEAYAALRAMPKLGNMPALANNKVKLLINGEETFGAIFQAIREAKKTILVQFFIIHDDKLGR
ELQSLLLEKAAEGVAIFVLYDRIGSHALPGAYIDKLRDGGVQIKAFATRGGWLNRFQINFRNHRKIVVVDGLKGYIGGHN
VGDEYMGLKPPLAPWRDTHVQVIGPVVACLQESFAEDWFWATRELPPLSLPDEFPEDGVLCQLLTSGPADAQETCSLFFV
EAIHAAEERVWITSPYFIPDEAVTAALTLAVLRGVDVRLLLPSRPDHYVVYAASSLYAFDAVRAGVRVFRYEPGFLHQKV
VLVDNEITAIGSANLDNRSFRLNFELMLLTVDSDFSSQVESMLTADFNLAREISVQESHETRRLHQLGMRVARLISPIL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=444, Percent_Identity=25.2252252252252, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1787007, Length=336, Percent_Identity=31.25, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI87081826, Length=389, Percent_Identity=27.7634961439589, Blast_Score=89, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 53726; Mature: 53726

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYHDPYFFGYVLGFIHLLGTGAAIHALLTVRTSQGAIAWAMPLLFIPYFTLLPYLVFGR
CCCCCCHHHHHHHHHHHHHCCCHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCC
SSFDAYIKARRQANQEMRIAIGDLNWRPWMEEAVAARRSEAYAALRAMPKLGNMPALANN
CCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
KVKLLINGEETFGAIFQAIREAKKTILVQFFIIHDDKLGRELQSLLLEKAAEGVAIFVLY
CEEEEECCCHHHHHHHHHHHHHHHEEEEEEEEEECCHHHHHHHHHHHHHHCCCEEEEEEE
DRIGSHALPGAYIDKLRDGGVQIKAFATRGGWLNRFQINFRNHRKIVVVDGLKGYIGGHN
ECCCCCCCCHHHHHHHCCCCEEEEEEEECCCCEEEEEEEECCCCEEEEEECCCCCCCCCC
VGDEYMGLKPPLAPWRDTHVQVIGPVVACLQESFAEDWFWATRELPPLSLPDEFPEDGVL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCE
CQLLTSGPADAQETCSLFFVEAIHAAEERVWITSPYFIPDEAVTAALTLAVLRGVDVRLL
EEEECCCCCCHHHHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEE
LPSRPDHYVVYAASSLYAFDAVRAGVRVFRYEPGFLHQKVVLVDNEITAIGSANLDNRSF
ECCCCCCEEEEEECHHHHHHHHHHCCEEEEECCCCCEEEEEEECCCEEEEECCCCCCCEE
RLNFELMLLTVDSDFSSQVESMLTADFNLAREISVQESHETRRLHQLGMRVARLISPIL
EEEEEEEEEEECCCHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDYHDPYFFGYVLGFIHLLGTGAAIHALLTVRTSQGAIAWAMPLLFIPYFTLLPYLVFGR
CCCCCCHHHHHHHHHHHHHCCCHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCC
SSFDAYIKARRQANQEMRIAIGDLNWRPWMEEAVAARRSEAYAALRAMPKLGNMPALANN
CCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
KVKLLINGEETFGAIFQAIREAKKTILVQFFIIHDDKLGRELQSLLLEKAAEGVAIFVLY
CEEEEECCCHHHHHHHHHHHHHHHEEEEEEEEEECCHHHHHHHHHHHHHHCCCEEEEEEE
DRIGSHALPGAYIDKLRDGGVQIKAFATRGGWLNRFQINFRNHRKIVVVDGLKGYIGGHN
ECCCCCCCCHHHHHHHCCCCEEEEEEEECCCCEEEEEEEECCCCEEEEEECCCCCCCCCC
VGDEYMGLKPPLAPWRDTHVQVIGPVVACLQESFAEDWFWATRELPPLSLPDEFPEDGVL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCE
CQLLTSGPADAQETCSLFFVEAIHAAEERVWITSPYFIPDEAVTAALTLAVLRGVDVRLL
EEEECCCCCCHHHHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEE
LPSRPDHYVVYAASSLYAFDAVRAGVRVFRYEPGFLHQKVVLVDNEITAIGSANLDNRSF
ECCCCCCEEEEEECHHHHHHHHHHCCEEEEECCCCCEEEEEEECCCEEEEECCCCCCCEE
RLNFELMLLTVDSDFSSQVESMLTADFNLAREISVQESHETRRLHQLGMRVARLISPIL
EEEEEEEEEEECCCHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]