Click here to switch to the map view.

The map label for this gene is phoB [H]

Identifier: 66048256

GI number: 66048256

Start: 5971022

End: 5971711

Strand: Direct

Name: phoB [H]

Synonym: Psyr_5032

Alternate gene names: 66048256

Gene position: 5971022-5971711 (Clockwise)

Preceding gene: 66048255

Following gene: 66048257

Centisome position: 97.99

GC content: 60.58

Gene sequence:

>690_bases
ATGGCTGGCAGGAGCATCCTGATCGTTGACGACGAAGCGCCAATTCGCGAAATGATCGCCGTTGCGTTGGAAATGGCTGG
ATACGACTGTATTGAAGCCGAAAACTCTCAGCAGGCCCACGCGATCATCGTGGACCGCAAGCCTGACCTGATTCTGCTCG
ACTGGATGCTGCCTGGCACGTCCGGCATCGAACTGGCCCGGCGCCTCAAGCGTGATGAACTGACCGGGGACATCCCGATC
ATCATGCTCACCGCCAAGGGTGAAGAGGACAACAAGATTCAGGGCCTGGAAGTCGGCGCCGACGATTACATCACCAAGCC
TTTCTCGCCCCGCGAGTTGGTGGCACGCCTCAAGGCTGTGCTGCGTCGCGCTGGCCCGACTGACGGCGAAGCGCCCATCG
AAGTCGGCGGCCTGCTGCTCGACCCGATCAGCCACCGGGTCACCATCGACGGCAAACCGGCCGAAATGGGCCCGACCGAA
TACCGTCTGCTGCAGTTCTTCATGACCCACCAGGAGCGCGCCTACACGCGCGGTCAGTTGCTGGATCAGGTATGGGGCGG
CAACGTCTACGTCGAGGAACGCACCGTCGATGTTCACATCCGCCGCCTGCGCAAGGCTTTGGGGGATGCGTATGAAAATC
TGGTACAAACCGTACGCGGCACTGGCTATCGTTTCTCGACCAAAAGCTGA

Upstream 100 bases:

>100_bases
AGCCTGTCAGCGTGCTAGCCTCATTCAGACCTGTCACACGACTGCAATAATTCCGTTATCTAATGCGCCCCAAGACAGTT
AAATGACAAGAGGTTCGAGC

Downstream 100 bases:

>100_bases
CAACCGCTGCGTCGACGCAGGGCAAGGGCCGGTGCAGACCAGACAAGGATTTACCCCTAATTGAATCAAAATTGGCACGG
CACTCTGATACGCCACATGT

Product: response regulator receiver:transcriptional regulatory protein, C-terminal

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MAGRSILIVDDEAPIREMIAVALEMAGYDCIEAENSQQAHAIIVDRKPDLILLDWMLPGTSGIELARRLKRDELTGDIPI
IMLTAKGEEDNKIQGLEVGADDYITKPFSPRELVARLKAVLRRAGPTDGEAPIEVGGLLLDPISHRVTIDGKPAEMGPTE
YRLLQFFMTHQERAYTRGQLLDQVWGGNVYVEERTVDVHIRRLRKALGDAYENLVQTVRGTGYRFSTKS

Sequences:

>Translated_229_residues
MAGRSILIVDDEAPIREMIAVALEMAGYDCIEAENSQQAHAIIVDRKPDLILLDWMLPGTSGIELARRLKRDELTGDIPI
IMLTAKGEEDNKIQGLEVGADDYITKPFSPRELVARLKAVLRRAGPTDGEAPIEVGGLLLDPISHRVTIDGKPAEMGPTE
YRLLQFFMTHQERAYTRGQLLDQVWGGNVYVEERTVDVHIRRLRKALGDAYENLVQTVRGTGYRFSTKS
>Mature_228_residues
AGRSILIVDDEAPIREMIAVALEMAGYDCIEAENSQQAHAIIVDRKPDLILLDWMLPGTSGIELARRLKRDELTGDIPII
MLTAKGEEDNKIQGLEVGADDYITKPFSPRELVARLKAVLRRAGPTDGEAPIEVGGLLLDPISHRVTIDGKPAEMGPTEY
RLLQFFMTHQERAYTRGQLLDQVWGGNVYVEERTVDVHIRRLRKALGDAYENLVQTVRGTGYRFSTKS

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=227, Percent_Identity=62.5550660792952, Blast_Score=292, Evalue=1e-80,
Organism=Escherichia coli, GI1788394, Length=228, Percent_Identity=39.9122807017544, Blast_Score=158, Evalue=3e-40,
Organism=Escherichia coli, GI1786784, Length=219, Percent_Identity=36.5296803652968, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1789809, Length=229, Percent_Identity=36.6812227074236, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1790863, Length=237, Percent_Identity=35.4430379746835, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI1786911, Length=224, Percent_Identity=36.6071428571429, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI2367329, Length=233, Percent_Identity=36.9098712446352, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI87082012, Length=221, Percent_Identity=33.9366515837104, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1790860, Length=226, Percent_Identity=34.070796460177, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1787375, Length=224, Percent_Identity=29.0178571428571, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1789402, Length=225, Percent_Identity=31.1111111111111, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1790552, Length=218, Percent_Identity=33.4862385321101, Blast_Score=113, Evalue=9e-27,
Organism=Escherichia coli, GI1787229, Length=225, Percent_Identity=34.2222222222222, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI145693140, Length=237, Percent_Identity=32.9113924050633, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1788550, Length=108, Percent_Identity=35.1851851851852, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1790299, Length=110, Percent_Identity=32.7272727272727, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788191, Length=121, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25584; Mature: 25453

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGRSILIVDDEAPIREMIAVALEMAGYDCIEAENSQQAHAIIVDRKPDLILLDWMLPGT
CCCCEEEEEECCCCHHHHHHHHHHHCCCCEECCCCCCCEEEEEEECCCCEEEEEEECCCC
SGIELARRLKRDELTGDIPIIMLTAKGEEDNKIQGLEVGADDYITKPFSPRELVARLKAV
CHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHH
LRRAGPTDGEAPIEVGGLLLDPISHRVTIDGKPAEMGPTEYRLLQFFMTHQERAYTRGQL
HHHCCCCCCCCCCEECCEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LDQVWGGNVYVEERTVDVHIRRLRKALGDAYENLVQTVRGTGYRFSTKS
HHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
>Mature Secondary Structure 
AGRSILIVDDEAPIREMIAVALEMAGYDCIEAENSQQAHAIIVDRKPDLILLDWMLPGT
CCCEEEEEECCCCHHHHHHHHHHHCCCCEECCCCCCCEEEEEEECCCCEEEEEEECCCC
SGIELARRLKRDELTGDIPIIMLTAKGEEDNKIQGLEVGADDYITKPFSPRELVARLKAV
CHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHH
LRRAGPTDGEAPIEVGGLLLDPISHRVTIDGKPAEMGPTEYRLLQFFMTHQERAYTRGQL
HHHCCCCCCCCCCEECCEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LDQVWGGNVYVEERTVDVHIRRLRKALGDAYENLVQTVRGTGYRFSTKS
HHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2115874; 10984043 [H]