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The map label for this gene is rfbB1 [H]

Identifier: 66048159

GI number: 66048159

Start: 5850272

End: 5851354

Strand: Direct

Name: rfbB1 [H]

Synonym: Psyr_4935

Alternate gene names: 66048159

Gene position: 5850272-5851354 (Clockwise)

Preceding gene: 66048153

Following gene: 66048160

Centisome position: 96.01

GC content: 61.03

Gene sequence:

>1083_bases
ATGCGAATACTCGTTACCGGGGGCGCAGGTTTCATCGGCTCTGCACTGATCCGCCACCTGATAAACAACACCGACCACGA
AGTGCTGAACTTCGACAAATTGACCTACGCGGGCAACCTTGAATCGCTGCAGTCGATTGCCACCGACACGCGCTATGAGT
TCGTGCAGGCGGATATCTGCGATCAGGCCCGTGTCAGCGCGGTGCTCGAGCGCTTTGCGCCGCAGGCGATCATGCACCTG
GCGGCTGAATCCCACGTCGACCGCTCCATCGATGGTCCGGCCGAGTTCATCCAGACCAACATTGTCGGCACGTACAGCCT
GCTGGAAGCCACGCGCGCCTGGTGGCTGAAACTGCCGGAAGCGCAGCGCCAGGCGTTCCGCTTTCACCATATTTCCACTG
ATGAGGTGTATGGCGACCTGCACGGTGTCGATGATCTGTTCACCGAAACCACACCGTATGCGCCCAGCTCACCTTACTCG
GCGAGCAAGGCGGCGTCCGACCACCTGGTGCGTGCCTGGCACCGTACCTATGGCCTGCCGGTGGTGGTGACCAACTGCTC
CAACAACTACGGGCCGTTCCACTTCCCGGAAAAGCTCATCCCGCTGGTGATTCTCAACGCCCTCGCGGGCAAGCCCCTGC
CGGTGTACGGCAATGGCCTGCAAGTGCGCGACTGGCTGTACGTCGAGGATCATGCGCGGGCACTGCTCAAGGTGGTGACC
GAAGGTGAAGTCGGCGAGACGTACAACATCGGTGGCCACAACGAGCAGAAAAACATCGACGTGGTGCGTGGTATCTGCGC
GCTGCTCGATGAGCTCGCCCCGCAGCACCCGGCAGGCGTTGCGCAGTACAGCGACCTGATCACCTACGTGGTCGACCGTC
CGGGCCACGATCAGCGTTATGCCATCGATGCCGGCAAGATCGACAAGGAGCTCGACTGGACGCCCGAAGAAACCTTCGAG
TCCGGGCTGCGCAAGACTGTGCAGTGGTACCTGGATAACCTGGACTGGTGCCGCAGGGTTCAGGACGGCAGTTATCAGGG
CGAACGACTGGGCTTTACCGAGCCTAAAGACCTGATTGCCTGA

Upstream 100 bases:

>100_bases
AGTTTTATTCAACCGCCTGCTCGGGTTTAATGGTTCGATCCTGTCATACGCGCTCAAGCGCTATGCTGCATTACTCCAGA
ACACACAAGAAGGTCAGTTC

Downstream 100 bases:

>100_bases
TCAGCACCCTTCTCGGCAGGCGTGCCTCAGGGCAGACTGTCGAGTCGCCACGACTTATTGAACATTGCAGGGGAAGCCAC
GTGAACGTAGTCGCAACAAA

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MRILVTGGAGFIGSALIRHLINNTDHEVLNFDKLTYAGNLESLQSIATDTRYEFVQADICDQARVSAVLERFAPQAIMHL
AAESHVDRSIDGPAEFIQTNIVGTYSLLEATRAWWLKLPEAQRQAFRFHHISTDEVYGDLHGVDDLFTETTPYAPSSPYS
ASKAASDHLVRAWHRTYGLPVVVTNCSNNYGPFHFPEKLIPLVILNALAGKPLPVYGNGLQVRDWLYVEDHARALLKVVT
EGEVGETYNIGGHNEQKNIDVVRGICALLDELAPQHPAGVAQYSDLITYVVDRPGHDQRYAIDAGKIDKELDWTPEETFE
SGLRKTVQWYLDNLDWCRRVQDGSYQGERLGFTEPKDLIA

Sequences:

>Translated_360_residues
MRILVTGGAGFIGSALIRHLINNTDHEVLNFDKLTYAGNLESLQSIATDTRYEFVQADICDQARVSAVLERFAPQAIMHL
AAESHVDRSIDGPAEFIQTNIVGTYSLLEATRAWWLKLPEAQRQAFRFHHISTDEVYGDLHGVDDLFTETTPYAPSSPYS
ASKAASDHLVRAWHRTYGLPVVVTNCSNNYGPFHFPEKLIPLVILNALAGKPLPVYGNGLQVRDWLYVEDHARALLKVVT
EGEVGETYNIGGHNEQKNIDVVRGICALLDELAPQHPAGVAQYSDLITYVVDRPGHDQRYAIDAGKIDKELDWTPEETFE
SGLRKTVQWYLDNLDWCRRVQDGSYQGERLGFTEPKDLIA
>Mature_360_residues
MRILVTGGAGFIGSALIRHLINNTDHEVLNFDKLTYAGNLESLQSIATDTRYEFVQADICDQARVSAVLERFAPQAIMHL
AAESHVDRSIDGPAEFIQTNIVGTYSLLEATRAWWLKLPEAQRQAFRFHHISTDEVYGDLHGVDDLFTETTPYAPSSPYS
ASKAASDHLVRAWHRTYGLPVVVTNCSNNYGPFHFPEKLIPLVILNALAGKPLPVYGNGLQVRDWLYVEDHARALLKVVT
EGEVGETYNIGGHNEQKNIDVVRGICALLDELAPQHPAGVAQYSDLITYVVDRPGHDQRYAIDAGKIDKELDWTPEETFE
SGLRKTVQWYLDNLDWCRRVQDGSYQGERLGFTEPKDLIA

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=343, Percent_Identity=35.8600583090379, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI42516563, Length=340, Percent_Identity=26.7647058823529, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI56237023, Length=278, Percent_Identity=27.3381294964029, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI56118217, Length=278, Percent_Identity=27.3381294964029, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI189083684, Length=278, Percent_Identity=27.3381294964029, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI4504031, Length=348, Percent_Identity=23.8505747126437, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI48994969, Length=351, Percent_Identity=72.9344729344729, Blast_Score=545, Evalue=1e-156,
Organism=Escherichia coli, GI1788353, Length=354, Percent_Identity=67.5141242937853, Blast_Score=497, Evalue=1e-142,
Organism=Escherichia coli, GI1786974, Length=355, Percent_Identity=27.3239436619718, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788366, Length=239, Percent_Identity=27.1966527196653, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788365, Length=349, Percent_Identity=24.0687679083095, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17568069, Length=338, Percent_Identity=36.9822485207101, Blast_Score=209, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI115532424, Length=330, Percent_Identity=32.1212121212121, Blast_Score=166, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17539532, Length=343, Percent_Identity=25.3644314868805, Blast_Score=101, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI71982035, Length=366, Percent_Identity=25.9562841530055, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71982038, Length=368, Percent_Identity=25.8152173913043, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17539422, Length=246, Percent_Identity=25.2032520325203, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI133901786, Length=246, Percent_Identity=25.2032520325203, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI133901788, Length=246, Percent_Identity=25.2032520325203, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17539424, Length=246, Percent_Identity=25.2032520325203, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI133901790, Length=246, Percent_Identity=25.2032520325203, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17507723, Length=188, Percent_Identity=26.5957446808511, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=363, Percent_Identity=22.8650137741047, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356223, Length=348, Percent_Identity=27.0114942528736, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI19923002, Length=363, Percent_Identity=26.9972451790634, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24158427, Length=337, Percent_Identity=25.2225519287834, Blast_Score=82, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40335; Mature: 40335

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILVTGGAGFIGSALIRHLINNTDHEVLNFDKLTYAGNLESLQSIATDTRYEFVQADIC
CEEEEECCCHHHHHHHHHHHHCCCCCHHHCHHHHEECCCHHHHHHHHHHHHHHHHHHHHH
DQARVSAVLERFAPQAIMHLAAESHVDRSIDGPAEFIQTNIVGTYSLLEATRAWWLKLPE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCH
AQRQAFRFHHISTDEVYGDLHGVDDLFTETTPYAPSSPYSASKAASDHLVRAWHRTYGLP
HHHHHHHHHCCCHHHHHHHHCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
VVVTNCSNNYGPFHFPEKLIPLVILNALAGKPLPVYGNGLQVRDWLYVEDHARALLKVVT
EEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEHHHHHHHHHHHH
EGEVGETYNIGGHNEQKNIDVVRGICALLDELAPQHPAGVAQYSDLITYVVDRPGHDQRY
CCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEE
AIDAGKIDKELDWTPEETFESGLRKTVQWYLDNLDWCRRVQDGSYQGERLGFTEPKDLIA
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCHHHCC
>Mature Secondary Structure
MRILVTGGAGFIGSALIRHLINNTDHEVLNFDKLTYAGNLESLQSIATDTRYEFVQADIC
CEEEEECCCHHHHHHHHHHHHCCCCCHHHCHHHHEECCCHHHHHHHHHHHHHHHHHHHHH
DQARVSAVLERFAPQAIMHLAAESHVDRSIDGPAEFIQTNIVGTYSLLEATRAWWLKLPE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCH
AQRQAFRFHHISTDEVYGDLHGVDDLFTETTPYAPSSPYSASKAASDHLVRAWHRTYGLP
HHHHHHHHHCCCHHHHHHHHCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
VVVTNCSNNYGPFHFPEKLIPLVILNALAGKPLPVYGNGLQVRDWLYVEDHARALLKVVT
EEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEHHHHHHHHHHHH
EGEVGETYNIGGHNEQKNIDVVRGICALLDELAPQHPAGVAQYSDLITYVVDRPGHDQRY
CCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEE
AIDAGKIDKELDWTPEETFESGLRKTVQWYLDNLDWCRRVQDGSYQGERLGFTEPKDLIA
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8022265; 10710307 [H]