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The map label for this gene is opuAB [H]

Identifier: 66048054

GI number: 66048054

Start: 5732872

End: 5733723

Strand: Direct

Name: opuAB [H]

Synonym: Psyr_4830

Alternate gene names: 66048054

Gene position: 5732872-5733723 (Clockwise)

Preceding gene: 66048053

Following gene: 66048055

Centisome position: 94.08

GC content: 61.62

Gene sequence:

>852_bases
ATGTTTCCTGAAAGCTTCACTTTCTCGATCGCCAACTGGGTCAACGATGGCGTCGACTCACTGGTCACGCAGTACGGCGA
TGTGTTCCGGCACATCTCCGACACCCTGCTATGGGCCATCGTCAACCTCGAAGGCCTGCTGCGCATGGCGCCGTGGTGGC
TGATGCTGATGATCGTCGGCGGCATTGCCTGGCACGCGACACGCAAGATCGTCACGACGCTGGTCATCGTCGGCCTGCTG
TTTCTGGTCGGTGCGGTCGGGTTGTGGGACAAGCTGATGCAGACCCTGGCGCTGATGCTGGTCGCCACGATCATCGCGGT
GCTGATCGGCATTCCGCTGGGCATTCTTTCGGCACGCAGCAATCGTCTGCGCTCGGTGCTGATGCCGCTGCTCGACATCA
TGCAGACCATGCCCAGCTTCGTTTACCTGATCCCGGTGCTGATGCTGTTCGGTCTGGGCAAGGTTCCGGCGATCTTCGCC
ACGGTGATTTACGCCGCGCCGCCGCTGATTCGTCTGACCGATCTGGGCATTCGTCAGGTGGATGGTGAAGTGATGGAAGC
CATCAACGCGTTCGGCGCCAACCGCTGGCAGCAACTGTTCGGTGTGCAACTGCCGCTGGCGATGCCGAGCATCATGGCCG
GGATCAACCAGACCACCATGATGGCGCTGTCGATGGTGGTGATTGCCTCGATGATCGGCGCACGCGGGCTTGGCGAAGAC
GTGCTGGTGGGCATTCAGACCCTCAACGTCGGGCGCGGGCTGGAAGCGGGCCTGGCCATTGTCATTCTGGCGGTCGTCAT
CGACCGTATCACGCAGGCCTATGGCCGCCCACGGCATGAGGCGCACAAATGA

Upstream 100 bases:

>100_bases
GCGAAGAAGGTGGACGCTTCGTTGTAAACGACCGGGCTGTCGGACTGAACGGTTCGACAGCCCTGTCGTTGACCCGACAC
ACCTCATTTGAGAGCCGCTT

Downstream 100 bases:

>100_bases
GCAGCGTGGAACCGAACAAAATCGTCGTCAAAAACGTGTTCAAGATCTTCGGCAACCGCTCCAAGGAAGCACTGCAGTTG
ATCCAGCAAAACCACAGCAA

Product: binding-protein dependent transport system inner membrane protein

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MFPESFTFSIANWVNDGVDSLVTQYGDVFRHISDTLLWAIVNLEGLLRMAPWWLMLMIVGGIAWHATRKIVTTLVIVGLL
FLVGAVGLWDKLMQTLALMLVATIIAVLIGIPLGILSARSNRLRSVLMPLLDIMQTMPSFVYLIPVLMLFGLGKVPAIFA
TVIYAAPPLIRLTDLGIRQVDGEVMEAINAFGANRWQQLFGVQLPLAMPSIMAGINQTTMMALSMVVIASMIGARGLGED
VLVGIQTLNVGRGLEAGLAIVILAVVIDRITQAYGRPRHEAHK

Sequences:

>Translated_283_residues
MFPESFTFSIANWVNDGVDSLVTQYGDVFRHISDTLLWAIVNLEGLLRMAPWWLMLMIVGGIAWHATRKIVTTLVIVGLL
FLVGAVGLWDKLMQTLALMLVATIIAVLIGIPLGILSARSNRLRSVLMPLLDIMQTMPSFVYLIPVLMLFGLGKVPAIFA
TVIYAAPPLIRLTDLGIRQVDGEVMEAINAFGANRWQQLFGVQLPLAMPSIMAGINQTTMMALSMVVIASMIGARGLGED
VLVGIQTLNVGRGLEAGLAIVILAVVIDRITQAYGRPRHEAHK
>Mature_283_residues
MFPESFTFSIANWVNDGVDSLVTQYGDVFRHISDTLLWAIVNLEGLLRMAPWWLMLMIVGGIAWHATRKIVTTLVIVGLL
FLVGAVGLWDKLMQTLALMLVATIIAVLIGIPLGILSARSNRLRSVLMPLLDIMQTMPSFVYLIPVLMLFGLGKVPAIFA
TVIYAAPPLIRLTDLGIRQVDGEVMEAINAFGANRWQQLFGVQLPLAMPSIMAGINQTTMMALSMVVIASMIGARGLGED
VLVGIQTLNVGRGLEAGLAIVILAVVIDRITQAYGRPRHEAHK

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=265, Percent_Identity=48.3018867924528, Blast_Score=218, Evalue=3e-58,
Organism=Escherichia coli, GI1788451, Length=195, Percent_Identity=32.8205128205128, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1788449, Length=174, Percent_Identity=28.735632183908, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786564, Length=152, Percent_Identity=32.2368421052632, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30842; Mature: 30842

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
6.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MFPESFTFSIANWVNDGVDSLVTQYGDVFRHISDTLLWAIVNLEGLLRMAPWWLMLMIVG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIAWHATRKIVTTLVIVGLLFLVGAVGLWDKLMQTLALMLVATIIAVLIGIPLGILSARS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRLRSVLMPLLDIMQTMPSFVYLIPVLMLFGLGKVPAIFATVIYAAPPLIRLTDLGIRQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
DGEVMEAINAFGANRWQQLFGVQLPLAMPSIMAGINQTTMMALSMVVIASMIGARGLGED
CHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHH
VLVGIQTLNVGRGLEAGLAIVILAVVIDRITQAYGRPRHEAHK
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]