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The map label for this gene is opuAB [H]

Identifier: 66047934

GI number: 66047934

Start: 5581152

End: 5581997

Strand: Direct

Name: opuAB [H]

Synonym: Psyr_4710

Alternate gene names: 66047934

Gene position: 5581152-5581997 (Clockwise)

Preceding gene: 66047933

Following gene: 66047935

Centisome position: 91.59

GC content: 59.34

Gene sequence:

>846_bases
ATGCTGACTGACCATAAAATCCCTCTGGGCGAGTACATCGCCGCATTTGTCGATTGGTTGACGGCCAACGGCGCCGATTA
CTTCGACGCGATAGCCTCGACACTGGAAATGATGATCCACGGGTTCACTTTCGCGCTGACCTGGTTCAATCCATTCGTAC
TGATCGGCCTTATCGCCGCCCTCGCGCACTTCATCCAGCGCAAGTGGGGCCTGACGGTTTTCGTCATCCTTTCGTTTCTG
CTGATCCTCAACCTGCATTATTGGCAGGAGACCATGGAAACCCTGGCGCAGGTGCTCTTCGCCACGCTGGTCTGTGTCGT
CATCGGCGTGCCGCTGGGCATCATTGCCGCGCACAAGCCGCTGTTCTACACCATCATCCGGCCGTTACTCGACCTGATGC
AGACCGTGCCCACCTTCGTCTATCTGATTCCGACGCTCACGCTGTTCGGTCTGGGCGTGGTACCCGGCCTGATCTCGACG
GTGGTTTTCGCCATTGCCGCGCCGATTCGCCTGACCTACCTGGGTATCCGCGATGTGCCTCAGGAGTTGCTGGATGCCGG
CAAGGCCTTCGGCTCGTCACGCCGCCAGTTGCTGACACGCATTGAACTGCCCTACGCAATGCCCAGCATTGCAGCCGGTA
TTACCCAGTGCATCATGCTGTCGCTGTCGATGGTGGTTATCGCCGCCCTGGTCGGCGCCGACGGCCTCGGCAAACCGGTA
GTCAACGCGCTGAACACAGCCGACATCGCGCTCGGTTTCGAAGCCGGTCTGGCAATCGTATTGCTGGCCATCATGCTCGA
CCGCATCTGCAAACAACCCGAAGCCAAGGTAGGGTCCGACGCATGA

Upstream 100 bases:

>100_bases
CTGGAGGCAGCCAAAGCCAAGTTGACCCAGTAAGCCGACAGCAGGCGGGACTGAACCGCCTGCTGCCCTCTTCACCTTCC
GCAAGCGGACACCCGAAATC

Downstream 100 bases:

>100_bases
GTATCATCAAATTCGACAAGGTAGACGTCATCTTCTCCAAGGACCCGCGTGAAGCCCTCAAGCTGCTCGATGAAGGCCTC
ACGCGTGACCAGATCCTCAA

Product: binding-protein dependent transport system inner membrane protein

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MLTDHKIPLGEYIAAFVDWLTANGADYFDAIASTLEMMIHGFTFALTWFNPFVLIGLIAALAHFIQRKWGLTVFVILSFL
LILNLHYWQETMETLAQVLFATLVCVVIGVPLGIIAAHKPLFYTIIRPLLDLMQTVPTFVYLIPTLTLFGLGVVPGLIST
VVFAIAAPIRLTYLGIRDVPQELLDAGKAFGSSRRQLLTRIELPYAMPSIAAGITQCIMLSLSMVVIAALVGADGLGKPV
VNALNTADIALGFEAGLAIVLLAIMLDRICKQPEAKVGSDA

Sequences:

>Translated_281_residues
MLTDHKIPLGEYIAAFVDWLTANGADYFDAIASTLEMMIHGFTFALTWFNPFVLIGLIAALAHFIQRKWGLTVFVILSFL
LILNLHYWQETMETLAQVLFATLVCVVIGVPLGIIAAHKPLFYTIIRPLLDLMQTVPTFVYLIPTLTLFGLGVVPGLIST
VVFAIAAPIRLTYLGIRDVPQELLDAGKAFGSSRRQLLTRIELPYAMPSIAAGITQCIMLSLSMVVIAALVGADGLGKPV
VNALNTADIALGFEAGLAIVLLAIMLDRICKQPEAKVGSDA
>Mature_281_residues
MLTDHKIPLGEYIAAFVDWLTANGADYFDAIASTLEMMIHGFTFALTWFNPFVLIGLIAALAHFIQRKWGLTVFVILSFL
LILNLHYWQETMETLAQVLFATLVCVVIGVPLGIIAAHKPLFYTIIRPLLDLMQTVPTFVYLIPTLTLFGLGVVPGLIST
VVFAIAAPIRLTYLGIRDVPQELLDAGKAFGSSRRQLLTRIELPYAMPSIAAGITQCIMLSLSMVVIAALVGADGLGKPV
VNALNTADIALGFEAGLAIVLLAIMLDRICKQPEAKVGSDA

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=279, Percent_Identity=42.2939068100358, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1788449, Length=195, Percent_Identity=27.1794871794872, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788451, Length=183, Percent_Identity=31.6939890710383, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1786564, Length=158, Percent_Identity=31.6455696202532, Blast_Score=71, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30456; Mature: 30456

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]