| Definition | Pseudomonas syringae pv. syringae B728a, complete genome. |
|---|---|
| Accession | NC_007005 |
| Length | 6,093,698 |
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The map label for this gene is dnaG [H]
Identifier: 66047867
GI number: 66047867
Start: 5500196
End: 5502151
Strand: Direct
Name: dnaG [H]
Synonym: Psyr_4640
Alternate gene names: 66047867
Gene position: 5500196-5502151 (Clockwise)
Preceding gene: 66047866
Following gene: 66047868
Centisome position: 90.26
GC content: 59.66
Gene sequence:
>1956_bases ATGGCCGGGCTGATTCCCCAGAGCTTTATCGACGACCTTCTGAACCGCACCGATATTGTCGATGTTGTCAGTTCGCGCGT GCAGCTCAAGAAAGCCGGGAAGAACTACACAGCGTGCTGCCCGTTCCACAAAGAGAAAACACCCTCTTTCAGCGTCAGCC CGGACAAGCAGTTCTATTACTGCTTCGGTTGCGGCGCAGGCGGCAACGCGCTCGGCTTCATCATGGACCACGACAACCTG GACTTCCCCCAGGCTGTCGAAGACCTGGCCAAGGCCGCCGGCATGGAAGTGCCTCGCGAACAAAGCGTAAAAGGCCAGAA GCCTCGGCAACCGACCGATTCCCCGCTCTACCCGCTGCTGACCGCCGCCGCCGAGTTCTATCGCCAGGCCCTGAAAAGCC ACCCGTCACGCAAGGCTGCGGTGGATTACCTCAAGGGCCGCGGGCTGTCCGGCGAAATCGCCAGGGACTTTGGCCTGGGC TTCGCGCCGCCCGGCTGGGACAATCTGTACAAGCACCTGAGCAGCGACTCCTTGCAGCAAAAGGTGATGATCGATGCCGG GCTGCTGATCGAGAACGCCGAAACCGGCAAACGCTATGACCGCTTCCGCGACCGGGTGATGTTTCCGATCCGTGATTCGC GCGGGCGCGTCATTGCCTTTGGCGGCCGTGTACTGGGCGATGACAAACCCAAGTACCTGAACTCCCCGGAAACACCGGTT TTCCACAAGGGCCAGGAGCTGTACGGGCTGTTCGAGGCGCGCAAATTCAATCGCAACCTCGATGAAATCATCGTCGTTGA AGGCTACATGGACGTCATCGCCCTGGCCCAGCAAGGCTTGCGCAATGCCGTGGCGACGCTGGGCACTGCGACCAGCGAAG AGCATTTGAAGCGCCTGTTCAGGGTCGTGCCCAACGTACTGTTCTGCTTCGACGGCGATCAGGCCGGACGTAACGCTGCA TGGCGTGCGCTGGAAGCGACCCTGTCGAACCTGCAGGACGGACGCCGGGCGCGCTTTCTGTTTCTTCCCGAAGGCGAAGA TCCGGACACACTGGTGCGCTCCGAAGGCACCGACGCGTTCAAGGCACGCATCAATCAGCACGCGCAGCCGCTGGCCGATT ATTTTTTCGAGCAACTGACCAAAGAGTCCGACCCGCGCTCGCTGGAGGGCAAGGCGCACATGGCCACCCTCGCGGCGCCA TTGATCGACAAAGTGCCCGGCGCGAACCTGCGTATTCTGATGCGTCAGCGCCTGACCGAAATTACCGGGCTGACCGGCGA GGCCGTCAGCCATCTGGTGCAGAGCGCGCCGGCAGAGGCGCCACCGAGCTACGACCCGTATGTCGACTACGACGCCATGC CTGATTTTGCCGATTACCAGCCACAAGGCGGTTACGAGCCTCAGCAGGAATGGACGCAAAAGAAAACCGGCGGTCAGAAT CAAAAAAAATGGGACAACAAATCCTGGGGCAAAAAAGGCAAGCGCCAGGACTTCGAACCTCGAACGCCGCGTGTACCCAC CGCCGTCGAGCCGCCCATGCAGGCCGCACTGAGAACCTTGCTGCATCACCCCGAATTGGCGGAAAAGGTCGAAAATGCCA GCCATTTCGCTGCAGAAGACCAATCCAACGCACAACTGCTGGTCGCTCTTATCGAGGCCCGGCAAAAGAATCCGAACCTT CGGTCGTTACAGTTGATAGCGCGATGGCACGGGACTGAACAGGGCCGCTTGCTGCGCGCACTGGCAGAAAAGGAATGGCT GATTGAGGCCGATAACCTTGAACAACAGTTTTTCGACACTATAACTAGCTTATCCGCTCGCCAGCGCGAGCGCAGTCTGG AACATCTGATCAGCAAGGCTCGGCAGACCGAGCTGAGCGCAGAAGAGAAAATTCAGCTTAGGGAACTGCTAAATCGCAAT GTTCCCGCACAAACCCCGACCTCAACTGGCGCGTGA
Upstream 100 bases:
>100_bases GTTTATTGCCGACGTCTTCAAAAAAGACGGCTGACAGGCTGACGGAACGCTCGTAGAGTCATCCGATACTTTTGCACCAC GCTTGAATGATGAGAACGCC
Downstream 100 bases:
>100_bases GGTCATAGCTCGGGTATAATCCTCGGCTTGTTTTTTGCCCGCCAAGACCTTCAGTGGATAGGGTGTTATGTCCGGAAAAG CGCAACAGCAATCTCGTATC
Product: DNA primase
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 651; Mature: 650
Protein sequence:
>651_residues MAGLIPQSFIDDLLNRTDIVDVVSSRVQLKKAGKNYTACCPFHKEKTPSFSVSPDKQFYYCFGCGAGGNALGFIMDHDNL DFPQAVEDLAKAAGMEVPREQSVKGQKPRQPTDSPLYPLLTAAAEFYRQALKSHPSRKAAVDYLKGRGLSGEIARDFGLG FAPPGWDNLYKHLSSDSLQQKVMIDAGLLIENAETGKRYDRFRDRVMFPIRDSRGRVIAFGGRVLGDDKPKYLNSPETPV FHKGQELYGLFEARKFNRNLDEIIVVEGYMDVIALAQQGLRNAVATLGTATSEEHLKRLFRVVPNVLFCFDGDQAGRNAA WRALEATLSNLQDGRRARFLFLPEGEDPDTLVRSEGTDAFKARINQHAQPLADYFFEQLTKESDPRSLEGKAHMATLAAP LIDKVPGANLRILMRQRLTEITGLTGEAVSHLVQSAPAEAPPSYDPYVDYDAMPDFADYQPQGGYEPQQEWTQKKTGGQN QKKWDNKSWGKKGKRQDFEPRTPRVPTAVEPPMQAALRTLLHHPELAEKVENASHFAAEDQSNAQLLVALIEARQKNPNL RSLQLIARWHGTEQGRLLRALAEKEWLIEADNLEQQFFDTITSLSARQRERSLEHLISKARQTELSAEEKIQLRELLNRN VPAQTPTSTGA
Sequences:
>Translated_651_residues MAGLIPQSFIDDLLNRTDIVDVVSSRVQLKKAGKNYTACCPFHKEKTPSFSVSPDKQFYYCFGCGAGGNALGFIMDHDNL DFPQAVEDLAKAAGMEVPREQSVKGQKPRQPTDSPLYPLLTAAAEFYRQALKSHPSRKAAVDYLKGRGLSGEIARDFGLG FAPPGWDNLYKHLSSDSLQQKVMIDAGLLIENAETGKRYDRFRDRVMFPIRDSRGRVIAFGGRVLGDDKPKYLNSPETPV FHKGQELYGLFEARKFNRNLDEIIVVEGYMDVIALAQQGLRNAVATLGTATSEEHLKRLFRVVPNVLFCFDGDQAGRNAA WRALEATLSNLQDGRRARFLFLPEGEDPDTLVRSEGTDAFKARINQHAQPLADYFFEQLTKESDPRSLEGKAHMATLAAP LIDKVPGANLRILMRQRLTEITGLTGEAVSHLVQSAPAEAPPSYDPYVDYDAMPDFADYQPQGGYEPQQEWTQKKTGGQN QKKWDNKSWGKKGKRQDFEPRTPRVPTAVEPPMQAALRTLLHHPELAEKVENASHFAAEDQSNAQLLVALIEARQKNPNL RSLQLIARWHGTEQGRLLRALAEKEWLIEADNLEQQFFDTITSLSARQRERSLEHLISKARQTELSAEEKIQLRELLNRN VPAQTPTSTGA >Mature_650_residues AGLIPQSFIDDLLNRTDIVDVVSSRVQLKKAGKNYTACCPFHKEKTPSFSVSPDKQFYYCFGCGAGGNALGFIMDHDNLD FPQAVEDLAKAAGMEVPREQSVKGQKPRQPTDSPLYPLLTAAAEFYRQALKSHPSRKAAVDYLKGRGLSGEIARDFGLGF APPGWDNLYKHLSSDSLQQKVMIDAGLLIENAETGKRYDRFRDRVMFPIRDSRGRVIAFGGRVLGDDKPKYLNSPETPVF HKGQELYGLFEARKFNRNLDEIIVVEGYMDVIALAQQGLRNAVATLGTATSEEHLKRLFRVVPNVLFCFDGDQAGRNAAW RALEATLSNLQDGRRARFLFLPEGEDPDTLVRSEGTDAFKARINQHAQPLADYFFEQLTKESDPRSLEGKAHMATLAAPL IDKVPGANLRILMRQRLTEITGLTGEAVSHLVQSAPAEAPPSYDPYVDYDAMPDFADYQPQGGYEPQQEWTQKKTGGQNQ KKWDNKSWGKKGKRQDFEPRTPRVPTAVEPPMQAALRTLLHHPELAEKVENASHFAAEDQSNAQLLVALIEARQKNPNLR SLQLIARWHGTEQGRLLRALAEKEWLIEADNLEQQFFDTITSLSARQRERSLEHLISKARQTELSAEEKIQLRELLNRNV PAQTPTSTGA
Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]
COG id: COG0358
COG function: function code L; DNA primase (bacterial type)
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 Toprim domain [H]
Homologues:
Organism=Escherichia coli, GI1789447, Length=424, Percent_Identity=54.7169811320755, Blast_Score=489, Evalue=1e-139,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR016136 - InterPro: IPR013264 - InterPro: IPR019475 - InterPro: IPR006295 - InterPro: IPR013173 - InterPro: IPR006171 - InterPro: IPR002694 [H]
Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]
EC number: 2.7.7.-
Molecular weight: Translated: 72925; Mature: 72794
Theoretical pI: Translated: 7.21; Mature: 7.21
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.8 %Cys (Translated Protein) 1.5 %Met (Translated Protein) 2.3 %Cys+Met (Translated Protein) 0.8 %Cys (Mature Protein) 1.4 %Met (Mature Protein) 2.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MAGLIPQSFIDDLLNRTDIVDVVSSRVQLKKAGKNYTACCPFHKEKTPSFSVSPDKQFYY CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCEEE CFGCGAGGNALGFIMDHDNLDFPQAVEDLAKAAGMEVPREQSVKGQKPRQPTDSPLYPLL EEEECCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCCHHHHH TAAAEFYRQALKSHPSRKAAVDYLKGRGLSGEIARDFGLGFAPPGWDNLYKHLSSDSLQQ HHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHCCCCCCCCHHHHHHHHCCHHHHH KVMIDAGLLIENAETGKRYDRFRDRVMFPIRDSRGRVIAFGGRVLGDDKPKYLNSPETPV HHHHHCCCEEECCCCCHHHHHHHHHEECEEECCCCCEEEECCEEECCCCCCCCCCCCCCC FHKGQELYGLFEARKFNRNLDEIIVVEGYMDVIALAQQGLRNAVATLGTATSEEHLKRLF CCCCHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH RVVPNVLFCFDGDQAGRNAAWRALEATLSNLQDGRRARFLFLPEGEDPDTLVRSEGTDAF HHHCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCCCHHH KARINQHAQPLADYFFEQLTKESDPRSLEGKAHMATLAAPLIDKVPGANLRILMRQRLTE HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH ITGLTGEAVSHLVQSAPAEAPPSYDPYVDYDAMPDFADYQPQGGYEPQQEWTQKKTGGQN HHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC QKKWDNKSWGKKGKRQDFEPRTPRVPTAVEPPMQAALRTLLHHPELAEKVENASHFAAED CCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHCCCC QSNAQLLVALIEARQKNPNLRSLQLIARWHGTEQGRLLRALAEKEWLIEADNLEQQFFDT CCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHEEECCCHHHHHHHH ITSLSARQRERSLEHLISKARQTELSAEEKIQLRELLNRNVPAQTPTSTGA HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCC >Mature Secondary Structure AGLIPQSFIDDLLNRTDIVDVVSSRVQLKKAGKNYTACCPFHKEKTPSFSVSPDKQFYY CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCEEE CFGCGAGGNALGFIMDHDNLDFPQAVEDLAKAAGMEVPREQSVKGQKPRQPTDSPLYPLL EEEECCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCCHHHHH TAAAEFYRQALKSHPSRKAAVDYLKGRGLSGEIARDFGLGFAPPGWDNLYKHLSSDSLQQ HHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHCCCCCCCCHHHHHHHHCCHHHHH KVMIDAGLLIENAETGKRYDRFRDRVMFPIRDSRGRVIAFGGRVLGDDKPKYLNSPETPV HHHHHCCCEEECCCCCHHHHHHHHHEECEEECCCCCEEEECCEEECCCCCCCCCCCCCCC FHKGQELYGLFEARKFNRNLDEIIVVEGYMDVIALAQQGLRNAVATLGTATSEEHLKRLF CCCCHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH RVVPNVLFCFDGDQAGRNAAWRALEATLSNLQDGRRARFLFLPEGEDPDTLVRSEGTDAF HHHCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCCCHHH KARINQHAQPLADYFFEQLTKESDPRSLEGKAHMATLAAPLIDKVPGANLRILMRQRLTE HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH ITGLTGEAVSHLVQSAPAEAPPSYDPYVDYDAMPDFADYQPQGGYEPQQEWTQKKTGGQN HHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC QKKWDNKSWGKKGKRQDFEPRTPRVPTAVEPPMQAALRTLLHHPELAEKVENASHFAAED CCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHCCCC QSNAQLLVALIEARQKNPNLRSLQLIARWHGTEQGRLLRALAEKEWLIEADNLEQQFFDT CCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHEEECCCHHHHHHHH ITSLSARQRERSLEHLISKARQTELSAEEKIQLRELLNRNVPAQTPTSTGA HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 12534463 [H]