Click here to switch to the map view.

The map label for this gene is tuf [H]

Identifier: 66047777

GI number: 66047777

Start: 5403640

End: 5404833

Strand: Reverse

Name: tuf [H]

Synonym: Psyr_4550

Alternate gene names: 66047777

Gene position: 5404833-5403640 (Counterclockwise)

Preceding gene: 66047778

Following gene: 161486726

Centisome position: 88.7

GC content: 54.44

Gene sequence:

>1194_bases
GTGGCTAAAGAAAAATTTGATCGTTCCCTCCCGCACGTCAACGTTGGCACCATTGGTCACGTTGACCATGGTAAAACCAC
GCTGACCGCTGCTCTGACTCGCGTCTGCTCCGAAGTTTTCGGTTCGGCTGCTGTTGAATTCGACAAGATCGACAGCGCAC
CGGAAGAAAAGGCTCGTGGTATTACCATCAACACCGCGCACGTCGAATACAAATCGTTGATCCGTCACTATGCTCACGTT
GACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACTGGTGCTGCCCAGATGGACGGCGCGATTCTGGTTTGTTC
GGCCGCTGATGGTCCGATGCCACAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTTGGCGTTCCGTACATCGTGGTCT
TCCTGAACAAGGCTGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTGCGCGATCTGCTGAGCACT
TACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCTGCTCGTATGGCTCTGGAAGGTAAAGACGACAACGAAAT
GGGCACCACTGCCGTTCGTAAACTGGTTGAAACACTGGACTCCTACATCCCAGAACCAGTTCGTGTTACCGACAAGCCGT
TCCTGATGCCGATCGAAGACGTATTCTCGATCTCCGGTCGCGGTACTGTTGTGACTGGTCGTATTGAGCGCGGTATCGTC
AAGGTTCAGGATCCACTGGAAATCGTTGGTCTGCGTGACACTACCGTCACCACCTGCACCGGTGTTGAAATGTTCCGCAA
ACTGCTCGACGAAGGTCGTGCTGGCGAGAACTGCGGCGTTCTGCTGCGCGGTACCAAGCGTGACGACGTTGAGCGTGGCC
AGGTTCTGGTCAAGCCAGGCAGCGTCAAGCCGCACACTCAGTTCGAAGCTGAAATCTACGTTCTGAGCAAAGAAGAAGGC
GGTCGTCACACTCCGTTCTTCAAAGGCTACCGTCCACAGTTCTACTTCCGTACTACCGACGTAACTGGTAGCTGCGAACT
GCCGGAAGGCGTAGAAATGGTTATGCCAGGTGATAACGTTAAAGTTTCCGTTACCCTGATCAAGCCAATCGCAATGGAAG
ATGGTCTGCGTTTCGCAATCCGTGAAGGCGGTCGTACCGTCGGCGCTGGCGTCGTAGCCAAAATCATCGCTTAA

Upstream 100 bases:

>100_bases
ATGGAATTCAAAAAATACAATACAGCTCCGTCGCACATCGTCGAAACTGTAACCAAAAAACAAGGCTGATTCAGCGCCTT
TAGGCTAGGAGTTATTTGTC

Downstream 100 bases:

>100_bases
GCGTTGAGTCCCAAAAAGAGCCCCCGCACTGCGGGGGCTTTTTTATTGGGTTGACACCTTGTTAGGTCGTCTATAGAATT
GCGCCTCCTTTTAACGGGCG

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu-A [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MAKEKFDRSLPHVNVGTIGHVDHGKTTLTAALTRVCSEVFGSAAVEFDKIDSAPEEKARGITINTAHVEYKSLIRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSRQVGVPYIVVFLNKADLVDDAELLELVEMEVRDLLST
YDFPGDDTPIIIGSARMALEGKDDNEMGTTAVRKLVETLDSYIPEPVRVTDKPFLMPIEDVFSISGRGTVVTGRIERGIV
KVQDPLEIVGLRDTTVTTCTGVEMFRKLLDEGRAGENCGVLLRGTKRDDVERGQVLVKPGSVKPHTQFEAEIYVLSKEEG
GRHTPFFKGYRPQFYFRTTDVTGSCELPEGVEMVMPGDNVKVSVTLIKPIAMEDGLRFAIREGGRTVGAGVVAKIIA

Sequences:

>Translated_397_residues
MAKEKFDRSLPHVNVGTIGHVDHGKTTLTAALTRVCSEVFGSAAVEFDKIDSAPEEKARGITINTAHVEYKSLIRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSRQVGVPYIVVFLNKADLVDDAELLELVEMEVRDLLST
YDFPGDDTPIIIGSARMALEGKDDNEMGTTAVRKLVETLDSYIPEPVRVTDKPFLMPIEDVFSISGRGTVVTGRIERGIV
KVQDPLEIVGLRDTTVTTCTGVEMFRKLLDEGRAGENCGVLLRGTKRDDVERGQVLVKPGSVKPHTQFEAEIYVLSKEEG
GRHTPFFKGYRPQFYFRTTDVTGSCELPEGVEMVMPGDNVKVSVTLIKPIAMEDGLRFAIREGGRTVGAGVVAKIIA
>Mature_396_residues
AKEKFDRSLPHVNVGTIGHVDHGKTTLTAALTRVCSEVFGSAAVEFDKIDSAPEEKARGITINTAHVEYKSLIRHYAHVD
CPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSRQVGVPYIVVFLNKADLVDDAELLELVEMEVRDLLSTY
DFPGDDTPIIIGSARMALEGKDDNEMGTTAVRKLVETLDSYIPEPVRVTDKPFLMPIEDVFSISGRGTVVTGRIERGIVK
VQDPLEIVGLRDTTVTTCTGVEMFRKLLDEGRAGENCGVLLRGTKRDDVERGQVLVKPGSVKPHTQFEAEIYVLSKEEGG
RHTPFFKGYRPQFYFRTTDVTGSCELPEGVEMVMPGDNVKVSVTLIKPIAMEDGLRFAIREGGRTVGAGVVAKIIA

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=396, Percent_Identity=55.3030303030303, Blast_Score=470, Evalue=1e-133,
Organism=Homo sapiens, GI4503475, Length=461, Percent_Identity=29.2841648590022, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI4503471, Length=464, Percent_Identity=30.6034482758621, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI5729864, Length=434, Percent_Identity=28.8018433179723, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI223555963, Length=434, Percent_Identity=28.8018433179723, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI54607086, Length=346, Percent_Identity=28.6127167630058, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI194018522, Length=341, Percent_Identity=28.1524926686217, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI194018520, Length=334, Percent_Identity=28.4431137724551, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI194097354, Length=334, Percent_Identity=28.4431137724551, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI46094014, Length=319, Percent_Identity=28.5266457680251, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI4503507, Length=270, Percent_Identity=28.1481481481481, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI82546879, Length=439, Percent_Identity=25.2847380410023, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1790412, Length=397, Percent_Identity=77.5818639798489, Blast_Score=615, Evalue=1e-177,
Organism=Escherichia coli, GI1789737, Length=397, Percent_Identity=77.5818639798489, Blast_Score=614, Evalue=1e-177,
Organism=Escherichia coli, GI2367247, Length=380, Percent_Identity=29.4736842105263, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI48994988, Length=238, Percent_Identity=33.6134453781513, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1789108, Length=322, Percent_Identity=29.1925465838509, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788922, Length=132, Percent_Identity=31.8181818181818, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=401, Percent_Identity=50.3740648379052, Blast_Score=380, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI25141371, Length=386, Percent_Identity=45.8549222797927, Blast_Score=345, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI17552884, Length=465, Percent_Identity=31.3978494623656, Blast_Score=158, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17569207, Length=465, Percent_Identity=31.3978494623656, Blast_Score=158, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI32566303, Length=446, Percent_Identity=30.4932735426009, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI115532067, Length=441, Percent_Identity=27.2108843537415, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI115532065, Length=441, Percent_Identity=27.2108843537415, Blast_Score=121, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17506081, Length=246, Percent_Identity=31.3008130081301, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI32566629, Length=336, Percent_Identity=27.0833333333333, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI32566301, Length=154, Percent_Identity=37.012987012987, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17509919, Length=282, Percent_Identity=25.531914893617, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=236, Percent_Identity=27.9661016949153, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=62.6262626262626, Blast_Score=504, Evalue=1e-144,
Organism=Saccharomyces cerevisiae, GI6325337, Length=449, Percent_Identity=29.8440979955457, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=449, Percent_Identity=29.8440979955457, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6322937, Length=159, Percent_Identity=31.4465408805031, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320377, Length=334, Percent_Identity=27.5449101796407, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6320863, Length=270, Percent_Identity=28.1481481481481, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323320, Length=241, Percent_Identity=29.4605809128631, Blast_Score=66, Evalue=8e-12,
Organism=Drosophila melanogaster, GI281363316, Length=394, Percent_Identity=55.3299492385787, Blast_Score=456, Evalue=1e-128,
Organism=Drosophila melanogaster, GI17864358, Length=394, Percent_Identity=55.3299492385787, Blast_Score=456, Evalue=1e-128,
Organism=Drosophila melanogaster, GI19921738, Length=387, Percent_Identity=50.3875968992248, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI45553807, Length=457, Percent_Identity=28.2275711159737, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI45553816, Length=457, Percent_Identity=28.2275711159737, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24651721, Length=457, Percent_Identity=28.2275711159737, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI17864154, Length=457, Percent_Identity=28.2275711159737, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24652838, Length=457, Percent_Identity=28.6652078774617, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI17137572, Length=457, Percent_Identity=28.6652078774617, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI19922690, Length=306, Percent_Identity=30.718954248366, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI45550900, Length=442, Percent_Identity=26.4705882352941, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI17137380, Length=343, Percent_Identity=28.8629737609329, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24647048, Length=270, Percent_Identity=27.037037037037, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24647046, Length=270, Percent_Identity=27.037037037037, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43387; Mature: 43256

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFDRSLPHVNVGTIGHVDHGKTTLTAALTRVCSEVFGSAAVEFDKIDSAPEEKARG
CCCHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCEEHHHCCCCCHHHHCC
ITINTAHVEYKSLIRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLSRQVGVPYIVVFLNKADLVDDAELLELVEMEVRDLLSTYDFPGDDTPIIIGSARMALE
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCEEEEE
GKDDNEMGTTAVRKLVETLDSYIPEPVRVTDKPFLMPIEDVFSISGRGTVVTGRIERGIV
CCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEHHHHHCCCCCCEEEEEECCCCEE
KVQDPLEIVGLRDTTVTTCTGVEMFRKLLDEGRAGENCGVLLRGTKRDDVERGQVLVKPG
EECCCEEEEECCCCCEEEEHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCEEEEECC
SVKPHTQFEAEIYVLSKEEGGRHTPFFKGYRPQFYFRTTDVTGSCELPEGVEMVMPGDNV
CCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEECCCCE
KVSVTLIKPIAMEDGLRFAIREGGRTVGAGVVAKIIA
EEEEEEEEEEECCCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKEKFDRSLPHVNVGTIGHVDHGKTTLTAALTRVCSEVFGSAAVEFDKIDSAPEEKARG
CCHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCEEHHHCCCCCHHHHCC
ITINTAHVEYKSLIRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLSRQVGVPYIVVFLNKADLVDDAELLELVEMEVRDLLSTYDFPGDDTPIIIGSARMALE
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCEEEEE
GKDDNEMGTTAVRKLVETLDSYIPEPVRVTDKPFLMPIEDVFSISGRGTVVTGRIERGIV
CCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEHHHHHCCCCCCEEEEEECCCCEE
KVQDPLEIVGLRDTTVTTCTGVEMFRKLLDEGRAGENCGVLLRGTKRDDVERGQVLVKPG
EECCCEEEEECCCCCEEEEHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCEEEEECC
SVKPHTQFEAEIYVLSKEEGGRHTPFFKGYRPQFYFRTTDVTGSCELPEGVEMVMPGDNV
CCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEECCCCE
KVSVTLIKPIAMEDGLRFAIREGGRTVGAGVVAKIIA
EEEEEEEEEEECCCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]