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The map label for this gene is gspE [C]

Identifier: 66047620

GI number: 66047620

Start: 5255165

End: 5256421

Strand: Direct

Name: gspE [C]

Synonym: Psyr_4393

Alternate gene names: 66047620

Gene position: 5255165-5256421 (Clockwise)

Preceding gene: 66047619

Following gene: 66047621

Centisome position: 86.24

GC content: 62.85

Gene sequence:

>1257_bases
ATGGCGGAGAAACTGTTTGGCGTCTCATCGCGCACGCAAGGCAAGCCTGACGACGGGCAAGGCCTGAAACTGGTGCTGCA
CCGTTACATCATCGACGGCATGGAAGAAAGCGGCAAAAGCCTGCTCGAAGGCTCACGACCGGCCCTGGCACAATTCGTCA
TCGACAAGGTGGCCGAATACGTCGCACGCCTGCGGCTGGCCATCTCGCGTTACGAAATGGAGCGCCTGGCTGAAGAACTG
GTCGACGAACTGACCGGCTTCGGCCCGCTGGAAGTGCTGCTGCGCGACACCTCGGTGACGGAAATTCTGGTCAACGGGCC
AGGCAAGGTGTTTGTCGAGCGCGACGGCGTGCTGCACCACACCGACCTGCGCTTTATCGATTCGCACCACGTCGAGCGCG
TGATGCAACGCATCCTGGCACCCCTTGGGCGGCGTCTGGACGAGTCTTCACCGATGGTCGACGCACGCCTGCCCGATGGC
AGCCGGGTCAACGCGATCATTCCGCCCATTGCGCTGGACGGCCCTTGCCTGTCGATCCGGAAATTCCGCAAGGACATGCT
CAAGAGCGCCGACCTGGTGGCCATGCAGACCATCGATCAGTCGATTTTCGAGTTCTTCCAGGAGGCCGTCGGCAAACGCT
GCAACATTCTGGTCAGCGGCGGCACCGGCACGGGCAAGACCACCATGCTCAACGTGCTGAGCCAGTTGATCGACAGCAAT
CAGCGTCTGGTGACCATCGAGGACGTTGCCGAGCTGCAGCTCAGTCACCCGCATGTGGTGCGCCTGGAAACCCGCCCGCC
CAACGCCGAAGGGCATGGCGAGGTGCGCGCCAGCGACCTGATCCGCAACTCGCTGCGGATGCGCCCGGACCGCATCATCC
TCGGTGAGATTCGCGGCGTCGAAGTGCTGGACGTATTGACGGCGATGAACACCGGCCACGACGGCTCGATGAGTACCGTG
CACGCCAACAATGCACAGGATGCCCTGCTGCGTCTGGAAACGCTGGTGGGCCTGACCGGGCGGCTGATCCCGGAAAAGAC
CCTGCGCCAGATGGTCTGCGCGGCACTGGATGTGATTATCCAGCTGACGCGCCTGCCTGACGGGCGCCGCTGTGTCAGTG
AAGTGGTGGAAGTGGTCGGCATCCGCGATGACATCTATGTCACCAATACCCTGTTTCGCGTTGATCGCCGCACCGGGGTC
GGTTTCATGCGCGAAGCGGCCCACGCTGCTGGCGACAAGCTGCGGCCTGGCTACTGA

Upstream 100 bases:

>100_bases
GCTCCGGGCTGCGGCGGCTTGGCGAGCATCTGGCACGGCATACCGAGGCTCGCGAGAAAGCCGATCAGGGCTGGCTCGCT
CGACTGTGGGGGAACCGGTA

Downstream 100 bases:

>100_bases
CATGCTCAAGGAGTCGGCATGAGTGCGTCTCTGGTACTGGGCCTGATCTCCTTGCTGCTGTTTATTGCGTCCATTCGCAT
GTTCTACCTGGCGCTCAATC

Product: type II secretion system protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MAEKLFGVSSRTQGKPDDGQGLKLVLHRYIIDGMEESGKSLLEGSRPALAQFVIDKVAEYVARLRLAISRYEMERLAEEL
VDELTGFGPLEVLLRDTSVTEILVNGPGKVFVERDGVLHHTDLRFIDSHHVERVMQRILAPLGRRLDESSPMVDARLPDG
SRVNAIIPPIALDGPCLSIRKFRKDMLKSADLVAMQTIDQSIFEFFQEAVGKRCNILVSGGTGTGKTTMLNVLSQLIDSN
QRLVTIEDVAELQLSHPHVVRLETRPPNAEGHGEVRASDLIRNSLRMRPDRIILGEIRGVEVLDVLTAMNTGHDGSMSTV
HANNAQDALLRLETLVGLTGRLIPEKTLRQMVCAALDVIIQLTRLPDGRRCVSEVVEVVGIRDDIYVTNTLFRVDRRTGV
GFMREAAHAAGDKLRPGY

Sequences:

>Translated_418_residues
MAEKLFGVSSRTQGKPDDGQGLKLVLHRYIIDGMEESGKSLLEGSRPALAQFVIDKVAEYVARLRLAISRYEMERLAEEL
VDELTGFGPLEVLLRDTSVTEILVNGPGKVFVERDGVLHHTDLRFIDSHHVERVMQRILAPLGRRLDESSPMVDARLPDG
SRVNAIIPPIALDGPCLSIRKFRKDMLKSADLVAMQTIDQSIFEFFQEAVGKRCNILVSGGTGTGKTTMLNVLSQLIDSN
QRLVTIEDVAELQLSHPHVVRLETRPPNAEGHGEVRASDLIRNSLRMRPDRIILGEIRGVEVLDVLTAMNTGHDGSMSTV
HANNAQDALLRLETLVGLTGRLIPEKTLRQMVCAALDVIIQLTRLPDGRRCVSEVVEVVGIRDDIYVTNTLFRVDRRTGV
GFMREAAHAAGDKLRPGY
>Mature_417_residues
AEKLFGVSSRTQGKPDDGQGLKLVLHRYIIDGMEESGKSLLEGSRPALAQFVIDKVAEYVARLRLAISRYEMERLAEELV
DELTGFGPLEVLLRDTSVTEILVNGPGKVFVERDGVLHHTDLRFIDSHHVERVMQRILAPLGRRLDESSPMVDARLPDGS
RVNAIIPPIALDGPCLSIRKFRKDMLKSADLVAMQTIDQSIFEFFQEAVGKRCNILVSGGTGTGKTTMLNVLSQLIDSNQ
RLVTIEDVAELQLSHPHVVRLETRPPNAEGHGEVRASDLIRNSLRMRPDRIILGEIRGVEVLDVLTAMNTGHDGSMSTVH
ANNAQDALLRLETLVGLTGRLIPEKTLRQMVCAALDVIIQLTRLPDGRRCVSEVVEVVGIRDDIYVTNTLFRVDRRTGVG
FMREAAHAAGDKLRPGY

Specific function: Involved In A General Secretion Pathway (Gsp) For The Export Of Proteins (By Similarity). [C]

COG id: COG4962

COG function: function code U; Flp pilus assembly protein, ATPase CpaF

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022399
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 46299; Mature: 46168

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKLFGVSSRTQGKPDDGQGLKLVLHRYIIDGMEESGKSLLEGSRPALAQFVIDKVAEY
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHH
VARLRLAISRYEMERLAEELVDELTGFGPLEVLLRDTSVTEILVNGPGKVFVERDGVLHH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEECCCEEEEEECCCCEEEEECCCEEEE
TDLRFIDSHHVERVMQRILAPLGRRLDESSPMVDARLPDGSRVNAIIPPIALDGPCLSIR
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEECCCCCCCCCHHHHH
KFRKDMLKSADLVAMQTIDQSIFEFFQEAVGKRCNILVSGGTGTGKTTMLNVLSQLIDSN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCC
QRLVTIEDVAELQLSHPHVVRLETRPPNAEGHGEVRASDLIRNSLRMRPDRIILGEIRGV
CCEEEHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCH
EVLDVLTAMNTGHDGSMSTVHANNAQDALLRLETLVGLTGRLIPEKTLRQMVCAALDVII
HHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
QLTRLPDGRRCVSEVVEVVGIRDDIYVTNTLFRVDRRTGVGFMREAAHAAGDKLRPGY
HHHCCCCHHHHHHHHHHHHCCCCCEEEEHHHHEEHHHCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AEKLFGVSSRTQGKPDDGQGLKLVLHRYIIDGMEESGKSLLEGSRPALAQFVIDKVAEY
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHH
VARLRLAISRYEMERLAEELVDELTGFGPLEVLLRDTSVTEILVNGPGKVFVERDGVLHH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEECCCEEEEEECCCCEEEEECCCEEEE
TDLRFIDSHHVERVMQRILAPLGRRLDESSPMVDARLPDGSRVNAIIPPIALDGPCLSIR
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEECCCCCCCCCHHHHH
KFRKDMLKSADLVAMQTIDQSIFEFFQEAVGKRCNILVSGGTGTGKTTMLNVLSQLIDSN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCC
QRLVTIEDVAELQLSHPHVVRLETRPPNAEGHGEVRASDLIRNSLRMRPDRIILGEIRGV
CCEEEHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCH
EVLDVLTAMNTGHDGSMSTVHANNAQDALLRLETLVGLTGRLIPEKTLRQMVCAALDVII
HHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
QLTRLPDGRRCVSEVVEVVGIRDDIYVTNTLFRVDRRTGVGFMREAAHAAGDKLRPGY
HHHCCCCHHHHHHHHHHHHCCCCCEEEEHHHHEEHHHCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036; 9634230 [H]