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The map label for this gene is pleD [H]

Identifier: 66047604

GI number: 66047604

Start: 5232552

End: 5234207

Strand: Reverse

Name: pleD [H]

Synonym: Psyr_4377

Alternate gene names: 66047604

Gene position: 5234207-5232552 (Counterclockwise)

Preceding gene: 66047611

Following gene: 66047601

Centisome position: 85.9

GC content: 60.08

Gene sequence:

>1656_bases
ATGACCGACCACGAAGACCCTAGCCGAGATCGCCTGAAGCATCACTTTGCCCAACGGGTCATTCACCAGGCGCGCCAGAT
TCTGGAGACGTGGCAGCGTCTGCAAAAGGCCGAATGGTCGGTCGGCGATATGGCCGAGCTCAAGGAGTCCACGCAGCGTC
TTTCGCGCTTCGCCGAGCGTTTCGAGCAGGCAGAACACAGCGCTCTGGCGCAGGAGATCAGCCAGGCCCTCGATGCGGTG
GAAGCCAATCGCGGTCGTCTCAACAGCCGTGTCATCACGGATCTCAATCGTCTGATGCAGCGCCTTTCCCGAACCGGACT
GCGGCACGGCGATCGTTTCGAGCAGACGGCGCTGCCACCGTTGCGCAAGCCCGTCTATATAGTGCTTCAGGATCACGAGC
GGGCCGAGCGCCTGGCCAAGCAGCTGGAGTTTTTCGGGCTGGCGGCGCTGTCGCTGCACAATGTCGCCGAGTTTCAGCGG
GCGATGGCACAGCGGCACCCGGCAGCGATTGTCATGGATGTCGATTTCAGCGGGCCGGGCAAGGGTCTTGAACTGGCCTC
TCAGGCTCAGGAAGGGCTGGAACAGAAGCTCCCGCTGTTGTTTTTCAGCCTCAATGAAACCGATACGCCCACCCGCCTGG
CTGCCGTCAGGGCCGGTGGCGAAGAGTTTCTGACCGGCGCGCTGGAAGCGTCGAGTCTGCTGGAAAAAATTGAAGTGCTG
ACCTGCGTGGCTCAATACGAGCCTTATAAAGTGCTGATCATCGACGATTCCCGCGCGCAGGCGCTGCACACCGAGCGTGT
TCTCAACGCCGCCGGAATCGTCACGCGGGTGTTGCTCGACCCGATTCAGGCGATGTCCGAACTGGCCGAGTTTCAGCCCG
ACCTGATCATTCTCGACATGTACATGCCGGGGTGTACCGGTACGGAGCTGGCCAAGGTCATTCGCCATAACGACCGTTAT
GTCAGCGTGCCGATCATTTATCTGTCGGCCGAAGACGATCAGGACAAGCAACTCGATGCCATGAGCGAAGGCGGCGACGA
TTTTCTGACCAAACCGATCAAACCCCGTCATCTGATCACCACGGTGCGCAACCGTGCTGCCAGGGCGCGAAGCCTCAAGG
CACGCATGGTCCGCGACAGCCTGACCGGGCTTTACAATCACACGCATATTTTGCAACTGCTGGAAGATTGCAGCTTTCGT
TCGCGACGTGAGGACAAGCCGCTGTGCTTTGCCATGCTCGATATCGACCACTTCAAGAAGGTCAACGACAGCCACGGCCA
CCCGATGGGGGACCGCGTCATCAAGAGCCTTGCGCTGTTTCTCAAGCAACGCCTGCGCAAAACCGACTTCATCGGGCGTT
ACGGCGGTGAGGAATTTGCCATTGTCATGCCGGACACCGACATCCAGAGCGCCCACAGGGTGCTGGATGACATCCGCCAT
CGCTTTGCAGAGATCCATTACCCGGCGCAGCCCGCCGATCTGTTCTGCACCTTCAGTGCCGGGGTGGTTTCTCTCGGTGC
CCATGACGACAGCCTGAGCCTTGCCTCCCAGGCGGATACGGCGTTGTACTGCGCCAAGCACGCCGGCCGCAATCGGGTGC
ATTCGGTGCTGGTCGATGTGCCTTATCACGTCGTATTGAACGGGATCGAAGCCTGA

Upstream 100 bases:

>100_bases
TGTTGTCAAATAAGTGACTTACGACGCTGTTCTGTTGTAACGGAGGTGCTAGCATAGCGCCATCAGCCGTATCGCTCAGC
AAAACCACAGTAGTCTGACC

Downstream 100 bases:

>100_bases
CTTCCGTTCAGCCCTGGCTTTCGCTCTGCCTTTGCCGCTCTGCCGATTTGCCCGGTGAAAATGCCAATGCCAATTGTGTG
CGGTTATGCATGTGAGTCAG

Product: GGDEF

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 551; Mature: 550

Protein sequence:

>551_residues
MTDHEDPSRDRLKHHFAQRVIHQARQILETWQRLQKAEWSVGDMAELKESTQRLSRFAERFEQAEHSALAQEISQALDAV
EANRGRLNSRVITDLNRLMQRLSRTGLRHGDRFEQTALPPLRKPVYIVLQDHERAERLAKQLEFFGLAALSLHNVAEFQR
AMAQRHPAAIVMDVDFSGPGKGLELASQAQEGLEQKLPLLFFSLNETDTPTRLAAVRAGGEEFLTGALEASSLLEKIEVL
TCVAQYEPYKVLIIDDSRAQALHTERVLNAAGIVTRVLLDPIQAMSELAEFQPDLIILDMYMPGCTGTELAKVIRHNDRY
VSVPIIYLSAEDDQDKQLDAMSEGGDDFLTKPIKPRHLITTVRNRAARARSLKARMVRDSLTGLYNHTHILQLLEDCSFR
SRREDKPLCFAMLDIDHFKKVNDSHGHPMGDRVIKSLALFLKQRLRKTDFIGRYGGEEFAIVMPDTDIQSAHRVLDDIRH
RFAEIHYPAQPADLFCTFSAGVVSLGAHDDSLSLASQADTALYCAKHAGRNRVHSVLVDVPYHVVLNGIEA

Sequences:

>Translated_551_residues
MTDHEDPSRDRLKHHFAQRVIHQARQILETWQRLQKAEWSVGDMAELKESTQRLSRFAERFEQAEHSALAQEISQALDAV
EANRGRLNSRVITDLNRLMQRLSRTGLRHGDRFEQTALPPLRKPVYIVLQDHERAERLAKQLEFFGLAALSLHNVAEFQR
AMAQRHPAAIVMDVDFSGPGKGLELASQAQEGLEQKLPLLFFSLNETDTPTRLAAVRAGGEEFLTGALEASSLLEKIEVL
TCVAQYEPYKVLIIDDSRAQALHTERVLNAAGIVTRVLLDPIQAMSELAEFQPDLIILDMYMPGCTGTELAKVIRHNDRY
VSVPIIYLSAEDDQDKQLDAMSEGGDDFLTKPIKPRHLITTVRNRAARARSLKARMVRDSLTGLYNHTHILQLLEDCSFR
SRREDKPLCFAMLDIDHFKKVNDSHGHPMGDRVIKSLALFLKQRLRKTDFIGRYGGEEFAIVMPDTDIQSAHRVLDDIRH
RFAEIHYPAQPADLFCTFSAGVVSLGAHDDSLSLASQADTALYCAKHAGRNRVHSVLVDVPYHVVLNGIEA
>Mature_550_residues
TDHEDPSRDRLKHHFAQRVIHQARQILETWQRLQKAEWSVGDMAELKESTQRLSRFAERFEQAEHSALAQEISQALDAVE
ANRGRLNSRVITDLNRLMQRLSRTGLRHGDRFEQTALPPLRKPVYIVLQDHERAERLAKQLEFFGLAALSLHNVAEFQRA
MAQRHPAAIVMDVDFSGPGKGLELASQAQEGLEQKLPLLFFSLNETDTPTRLAAVRAGGEEFLTGALEASSLLEKIEVLT
CVAQYEPYKVLIIDDSRAQALHTERVLNAAGIVTRVLLDPIQAMSELAEFQPDLIILDMYMPGCTGTELAKVIRHNDRYV
SVPIIYLSAEDDQDKQLDAMSEGGDDFLTKPIKPRHLITTVRNRAARARSLKARMVRDSLTGLYNHTHILQLLEDCSFRS
RREDKPLCFAMLDIDHFKKVNDSHGHPMGDRVIKSLALFLKQRLRKTDFIGRYGGEEFAIVMPDTDIQSAHRVLDDIRHR
FAEIHYPAQPADLFCTFSAGVVSLGAHDDSLSLASQADTALYCAKHAGRNRVHSVLVDVPYHVVLNGIEA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1786584, Length=173, Percent_Identity=35.8381502890173, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1787262, Length=161, Percent_Identity=35.4037267080745, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI145693134, Length=156, Percent_Identity=32.6923076923077, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI87082007, Length=176, Percent_Identity=35.2272727272727, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI87081881, Length=155, Percent_Identity=33.5483870967742, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1787802, Length=163, Percent_Identity=31.9018404907975, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788381, Length=126, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI87081974, Length=175, Percent_Identity=29.1428571428571, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1787816, Length=201, Percent_Identity=29.8507462686567, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1787541, Length=159, Percent_Identity=28.9308176100629, Blast_Score=73, Evalue=6e-14,
Organism=Escherichia coli, GI1788085, Length=160, Percent_Identity=30, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1786599, Length=114, Percent_Identity=26.3157894736842, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 62178; Mature: 62047

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDHEDPSRDRLKHHFAQRVIHQARQILETWQRLQKAEWSVGDMAELKESTQRLSRFAER
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
FEQAEHSALAQEISQALDAVEANRGRLNSRVITDLNRLMQRLSRTGLRHGDRFEQTALPP
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCC
LRKPVYIVLQDHERAERLAKQLEFFGLAALSLHNVAEFQRAMAQRHPAAIVMDVDFSGPG
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
KGLELASQAQEGLEQKLPLLFFSLNETDTPTRLAAVRAGGEEFLTGALEASSLLEKIEVL
CCHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
TCVAQYEPYKVLIIDDSRAQALHTERVLNAAGIVTRVLLDPIQAMSELAEFQPDLIILDM
HHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
YMPGCTGTELAKVIRHNDRYVSVPIIYLSAEDDQDKQLDAMSEGGDDFLTKPIKPRHLIT
ECCCCCHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHCCCCHHHCCCCCCHHHHH
TVRNRAARARSLKARMVRDSLTGLYNHTHILQLLEDCSFRSRREDKPLCFAMLDIDHFKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEEECHHHHHH
VNDSHGHPMGDRVIKSLALFLKQRLRKTDFIGRYGGEEFAIVMPDTDIQSAHRVLDDIRH
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHH
RFAEIHYPAQPADLFCTFSAGVVSLGAHDDSLSLASQADTALYCAKHAGRNRVHSVLVDV
HHHHCCCCCCCHHEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
PYHVVLNGIEA
CHHHHHCCCCC
>Mature Secondary Structure 
TDHEDPSRDRLKHHFAQRVIHQARQILETWQRLQKAEWSVGDMAELKESTQRLSRFAER
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
FEQAEHSALAQEISQALDAVEANRGRLNSRVITDLNRLMQRLSRTGLRHGDRFEQTALPP
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCC
LRKPVYIVLQDHERAERLAKQLEFFGLAALSLHNVAEFQRAMAQRHPAAIVMDVDFSGPG
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
KGLELASQAQEGLEQKLPLLFFSLNETDTPTRLAAVRAGGEEFLTGALEASSLLEKIEVL
CCHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
TCVAQYEPYKVLIIDDSRAQALHTERVLNAAGIVTRVLLDPIQAMSELAEFQPDLIILDM
HHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
YMPGCTGTELAKVIRHNDRYVSVPIIYLSAEDDQDKQLDAMSEGGDDFLTKPIKPRHLIT
ECCCCCHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHCCCCHHHCCCCCCHHHHH
TVRNRAARARSLKARMVRDSLTGLYNHTHILQLLEDCSFRSRREDKPLCFAMLDIDHFKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEEECHHHHHH
VNDSHGHPMGDRVIKSLALFLKQRLRKTDFIGRYGGEEFAIVMPDTDIQSAHRVLDDIRH
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHH
RFAEIHYPAQPADLFCTFSAGVVSLGAHDDSLSLASQADTALYCAKHAGRNRVHSVLVDV
HHHHCCCCCCCHHEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
PYHVVLNGIEA
CHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA