Click here to switch to the map view.

The map label for this gene is rluE [H]

Identifier: 66047563

GI number: 66047563

Start: 5187591

End: 5188223

Strand: Reverse

Name: rluE [H]

Synonym: Psyr_4336

Alternate gene names: 66047563

Gene position: 5188223-5187591 (Counterclockwise)

Preceding gene: 66047564

Following gene: 66047561

Centisome position: 85.14

GC content: 64.93

Gene sequence:

>633_bases
ATGTCCCGCACCCAACGCCCCGTTTCCCGCCGTCCTGCCTCCAAACCGGCAGGTGCTGCCCGGCGTGTCGCCAAAGCACC
ACCGGCCGAGCCCAGGCTGATCCTGTTCAACAAGCCTTTCGACGTGCTGACCCAGTTCAGCGATGAGGAAGGGCGGGCGA
CGCTCAAGGACTTCATCAAGATCCCTGGCATCTATCCGGCCGGGCGTCTGGACCGCGACAGCGAAGGATTGCTGTTACTG
ACCAACGACGGCCAGTTGCAGGCGCGCATTGCCGATCCGAAACACAAGCTGGCCAAGACCTACTGGGTGCAGGTCGAAGG
CGAGCCGAGCGATGAGCAGTTGCAGCAGCTGCGCGACGGTGTCCAGCTCAACGACGGGCCGACCTTGCCCGCCGAAGCGC
GGCAGCTTGATGAACCGCAACTCTGGCCGCGCAACCCGCCCGTGCGCTTTCGCAAGAGCGTGCCGACAAGCTGGCTGGAG
CTGGTGATCAAGGAAGGGCGTAATCGGCAGGTGCGGCGCATGACCGCTGCCGTCGGCTTGCCGACCCTGCGGCTGGTGCG
GGTCAGGATTGGCGACTGGACGCTGGAGGGCCTGGATCAGGGCCAGTACAGGGAAGTCGCCGCAAAACTGTGA

Upstream 100 bases:

>100_bases
GACGAGCCGCCATTCCGTTGATGGCTTGCCGTTAATAGATTGGTATTTGCACGAAGGGCCACTAAAGTGGCCCTTTCTGT
TTGCCGATTGCCGAATTCTC

Downstream 100 bases:

>100_bases
AGCCGGTGTTGGCTCAGTTGCGCAGGTGAGTCAGCGGCATTTCGGTGCTGGCCAGCACCTGATTGAGCACAAAGCTCGAT
TTGACGCTGGTCACGCCTTC

Product: pseudouridine synthase, Rsu

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MSRTQRPVSRRPASKPAGAARRVAKAPPAEPRLILFNKPFDVLTQFSDEEGRATLKDFIKIPGIYPAGRLDRDSEGLLLL
TNDGQLQARIADPKHKLAKTYWVQVEGEPSDEQLQQLRDGVQLNDGPTLPAEARQLDEPQLWPRNPPVRFRKSVPTSWLE
LVIKEGRNRQVRRMTAAVGLPTLRLVRVRIGDWTLEGLDQGQYREVAAKL

Sequences:

>Translated_210_residues
MSRTQRPVSRRPASKPAGAARRVAKAPPAEPRLILFNKPFDVLTQFSDEEGRATLKDFIKIPGIYPAGRLDRDSEGLLLL
TNDGQLQARIADPKHKLAKTYWVQVEGEPSDEQLQQLRDGVQLNDGPTLPAEARQLDEPQLWPRNPPVRFRKSVPTSWLE
LVIKEGRNRQVRRMTAAVGLPTLRLVRVRIGDWTLEGLDQGQYREVAAKL
>Mature_209_residues
SRTQRPVSRRPASKPAGAARRVAKAPPAEPRLILFNKPFDVLTQFSDEEGRATLKDFIKIPGIYPAGRLDRDSEGLLLLT
NDGQLQARIADPKHKLAKTYWVQVEGEPSDEQLQQLRDGVQLNDGPTLPAEARQLDEPQLWPRNPPVRFRKSVPTSWLEL
VIKEGRNRQVRRMTAAVGLPTLRLVRVRIGDWTLEGLDQGQYREVAAKL

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=205, Percent_Identity=58.5365853658537, Blast_Score=241, Evalue=2e-65,
Organism=Escherichia coli, GI1788510, Length=178, Percent_Identity=36.5168539325843, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787524, Length=182, Percent_Identity=35.7142857142857, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1790453, Length=176, Percent_Identity=30.1136363636364, Blast_Score=74, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 23726; Mature: 23594

Theoretical pI: Translated: 10.74; Mature: 10.74

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRTQRPVSRRPASKPAGAARRVAKAPPAEPRLILFNKPFDVLTQFSDEEGRATLKDFIK
CCCCCCCHHCCCCCCCCHHHHHHHCCCCCCCEEEEEECCHHHHHHCCCCCCHHHHHHHHH
IPGIYPAGRLDRDSEGLLLLTNDGQLQARIADPKHKLAKTYWVQVEGEPSDEQLQQLRDG
CCCCCCCCCCCCCCCCEEEEECCCEEEEEECCCHHHHCEEEEEEEECCCCHHHHHHHHCC
VQLNDGPTLPAEARQLDEPQLWPRNPPVRFRKSVPTSWLELVIKEGRNRQVRRMTAAVGL
CEECCCCCCCHHHHHCCCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHCC
PTLRLVRVRIGDWTLEGLDQGQYREVAAKL
CCEEEEEEEECCEEECCCCCCHHHHHHHCC
>Mature Secondary Structure 
SRTQRPVSRRPASKPAGAARRVAKAPPAEPRLILFNKPFDVLTQFSDEEGRATLKDFIK
CCCCCCHHCCCCCCCCHHHHHHHCCCCCCCEEEEEECCHHHHHHCCCCCCHHHHHHHHH
IPGIYPAGRLDRDSEGLLLLTNDGQLQARIADPKHKLAKTYWVQVEGEPSDEQLQQLRDG
CCCCCCCCCCCCCCCCEEEEECCCEEEEEECCCHHHHCEEEEEEEECCCCHHHHHHHHCC
VQLNDGPTLPAEARQLDEPQLWPRNPPVRFRKSVPTSWLELVIKEGRNRQVRRMTAAVGL
CEECCCCCCCHHHHHCCCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHCC
PTLRLVRVRIGDWTLEGLDQGQYREVAAKL
CCEEEEEEEECCEEECCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]