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The map label for this gene is alaS [H]

Identifier: 66047550

GI number: 66047550

Start: 5175676

End: 5176467

Strand: Reverse

Name: alaS [H]

Synonym: Psyr_4323

Alternate gene names: 66047550

Gene position: 5176467-5175676 (Counterclockwise)

Preceding gene: 66047551

Following gene: 66047549

Centisome position: 84.95

GC content: 48.99

Gene sequence:

>792_bases
ATGATCGCCACGACTCACGATGTGTTGCGTAACACGGAAAAACTGTATTACAAGGATCGGTACTTGGGGTCTACAAATAC
CAAAGTGACGCGGTTGTACCCAGACGCTATTGAGCTTGATTGTACGGTTGCCTACCCCGAGGGAGGTGGGCAGGAAGCTG
ACTTCGGTACTATAGAATTACCCATTGGTACCGTGCGATTTGTCTGGGTAAAAAAGCTCTACGGCACCCCCATTCACCTG
GAAGGCTTCAAGGGCGGAAAGTTAGGCGGAGTTATCCTCCATATGATACATCCTGAGGATCTTCACCTCCTTGACTCAAT
AAGTGAAGGCATGGCTGCTCTGATTAGAATTGATATTTTGCGTCGCGAACGATTGAGTCTATCTCACAGCGCTAGCCATT
TTCTGTATGCCGCTGCCACCAGCCTGAGCGAGGAACTAAAGCAGTGGACCATCGGCTGTCACATTAAAGAAAACTCGGCA
CGCTTTGATTTTCTTGTTGAAGAGTCCTTTAGCAGCGATGACATCAGTGAAATAGAACGACTCGCAAATGAGTTGATCGC
CCACGACGACGTGATCAAGAGCTATACGGTCTTTGATGTTGAGGATGCGCGCATGTGGAGTTACCAAGGCATCGAGATTC
CCTGCGGTGGCACTCATCTCGTTAATCCTAGTGCCATAGGCCGCCTGAACGTACGTCGAAAAAGGTTGGGTAAAGGTAAA
GAACGACTGATCTGTGACTTTCCCAACGCTGAAATTGATTTGTCCCCCTATCATGAGCGCGCCCTACGATGA

Upstream 100 bases:

>100_bases
GTGACGATGCATTCAGTGGACCGCGCTGTATCATCACCCAAGACGTTCCCTCGGGTGCTCGAGTGCAAGTGAAAACATCA
CTGCAGGTGGTAATTGAGCA

Downstream 100 bases:

>100_bases
GTACGCTTCCGCGACGCACCTATCTGTATTTCATCGCGCAATCCATTAACCTGACCACCGCTGTAATGTCCGTGACCATG
GCCGCTATTGTCGGCTCCGC

Product: synthetase, putative

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MIATTHDVLRNTEKLYYKDRYLGSTNTKVTRLYPDAIELDCTVAYPEGGGQEADFGTIELPIGTVRFVWVKKLYGTPIHL
EGFKGGKLGGVILHMIHPEDLHLLDSISEGMAALIRIDILRRERLSLSHSASHFLYAAATSLSEELKQWTIGCHIKENSA
RFDFLVEESFSSDDISEIERLANELIAHDDVIKSYTVFDVEDARMWSYQGIEIPCGGTHLVNPSAIGRLNVRRKRLGKGK
ERLICDFPNAEIDLSPYHERALR

Sequences:

>Translated_263_residues
MIATTHDVLRNTEKLYYKDRYLGSTNTKVTRLYPDAIELDCTVAYPEGGGQEADFGTIELPIGTVRFVWVKKLYGTPIHL
EGFKGGKLGGVILHMIHPEDLHLLDSISEGMAALIRIDILRRERLSLSHSASHFLYAAATSLSEELKQWTIGCHIKENSA
RFDFLVEESFSSDDISEIERLANELIAHDDVIKSYTVFDVEDARMWSYQGIEIPCGGTHLVNPSAIGRLNVRRKRLGKGK
ERLICDFPNAEIDLSPYHERALR
>Mature_263_residues
MIATTHDVLRNTEKLYYKDRYLGSTNTKVTRLYPDAIELDCTVAYPEGGGQEADFGTIELPIGTVRFVWVKKLYGTPIHL
EGFKGGKLGGVILHMIHPEDLHLLDSISEGMAALIRIDILRRERLSLSHSASHFLYAAATSLSEELKQWTIGCHIKENSA
RFDFLVEESFSSDDISEIERLANELIAHDDVIKSYTVFDVEDARMWSYQGIEIPCGGTHLVNPSAIGRLNVRRKRLGKGK
ERLICDFPNAEIDLSPYHERALR

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR022429
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 29703; Mature: 29703

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIATTHDVLRNTEKLYYKDRYLGSTNTKVTRLYPDAIELDCTVAYPEGGGQEADFGTIEL
CCCCCHHHHHCCHHHEEEHHCCCCCCCEEEEECCCCEEEEEEEECCCCCCCCCCCCEEEE
PIGTVRFVWVKKLYGTPIHLEGFKGGKLGGVILHMIHPEDLHLLDSISEGMAALIRIDIL
CCCHHHHEEHHHHHCCCEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
RRERLSLSHSASHFLYAAATSLSEELKQWTIGCHIKENSARFDFLVEESFSSDDISEIER
HHHHHCCCCCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCEEEEEEECCCCCCCHHHHHH
LANELIAHDDVIKSYTVFDVEDARMWSYQGIEIPCGGTHLVNPSAIGRLNVRRKRLGKGK
HHHHHHHHHHHHHEEEEEECCCCEEEEECCEEECCCCCEEECCHHHCCHHHHHHHCCCCC
ERLICDFPNAEIDLSPYHERALR
CEEEEECCCCEEECCCHHHHHCC
>Mature Secondary Structure
MIATTHDVLRNTEKLYYKDRYLGSTNTKVTRLYPDAIELDCTVAYPEGGGQEADFGTIEL
CCCCCHHHHHCCHHHEEEHHCCCCCCCEEEEECCCCEEEEEEEECCCCCCCCCCCCEEEE
PIGTVRFVWVKKLYGTPIHLEGFKGGKLGGVILHMIHPEDLHLLDSISEGMAALIRIDIL
CCCHHHHEEHHHHHCCCEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
RRERLSLSHSASHFLYAAATSLSEELKQWTIGCHIKENSARFDFLVEESFSSDDISEIER
HHHHHCCCCCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCEEEEEEECCCCCCCHHHHHH
LANELIAHDDVIKSYTVFDVEDARMWSYQGIEIPCGGTHLVNPSAIGRLNVRRKRLGKGK
HHHHHHHHHHHHHEEEEEECCCCEEEEECCEEECCCCCEEECCHHHCCHHHHHHHCCCCC
ERLICDFPNAEIDLSPYHERALR
CEEEEECCCCEEECCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA