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The map label for this gene is mscL [H]

Identifier: 66047503

GI number: 66047503

Start: 5101077

End: 5101523

Strand: Direct

Name: mscL [H]

Synonym: Psyr_4276

Alternate gene names: 66047503

Gene position: 5101077-5101523 (Clockwise)

Preceding gene: 66047501

Following gene: 66047505

Centisome position: 83.71

GC content: 58.17

Gene sequence:

>447_bases
ATGAGCGTGCTTAAGGAATTCAAGGCATTCGCGGTCAAAGGCAACGTAGTTGATATGGCCGTCGGTATTATCATCGGCGC
GGCATTCGGCAAGATCGTTTCTTCGTTCGTCGGCGATGTCATCATGCCGCCGCTGGGCCTGTTGATTGGCGGTGTCGACT
TCAGCGACCTGGCCGTCACCCTGCGTCCTGCGCAAGGGACTGCACCTGCGGTACTGCTGGCGTACGGCAAGTTCATCCAG
ACCGTCATCGACTTCATCATCGTCGCGTTCGCGATATTCATGGGCGTCAAGGCCATCAACCGCCTGAAACGCGAAGAAGC
CAAAGCCCCTACCCTGCCACCAACGCCCTCCAAGCAGGAAATCCTGCTGGGCGAGATTCGCGACCTGCTCAAAGAGCAGA
GCAAACCGGCAGCGCCCATTACCGTGGATCCTGCGCGTACGCTTTGA

Upstream 100 bases:

>100_bases
ACGCGAACGACTTCGGTCGATTGAACGTGGCACGGGCGGCAGCAGTACGTCTGGGCTACACTCATCCGGATCAAATTTAT
CTGCTACAAGGAATTACCCC

Downstream 100 bases:

>100_bases
TTGAGCAAGGCCCGCGAGCTATCGTCCCCATGCTCCGCGTGGGGATGCATTTGCGGTCGCTCTGCGTCCAGCGTTGAGTG
CGGTGACGCAGAGCGTCACG

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 148; Mature: 147

Protein sequence:

>148_residues
MSVLKEFKAFAVKGNVVDMAVGIIIGAAFGKIVSSFVGDVIMPPLGLLIGGVDFSDLAVTLRPAQGTAPAVLLAYGKFIQ
TVIDFIIVAFAIFMGVKAINRLKREEAKAPTLPPTPSKQEILLGEIRDLLKEQSKPAAPITVDPARTL

Sequences:

>Translated_148_residues
MSVLKEFKAFAVKGNVVDMAVGIIIGAAFGKIVSSFVGDVIMPPLGLLIGGVDFSDLAVTLRPAQGTAPAVLLAYGKFIQ
TVIDFIIVAFAIFMGVKAINRLKREEAKAPTLPPTPSKQEILLGEIRDLLKEQSKPAAPITVDPARTL
>Mature_147_residues
SVLKEFKAFAVKGNVVDMAVGIIIGAAFGKIVSSFVGDVIMPPLGLLIGGVDFSDLAVTLRPAQGTAPAVLLAYGKFIQT
VIDFIIVAFAIFMGVKAINRLKREEAKAPTLPPTPSKQEILLGEIRDLLKEQSKPAAPITVDPARTL

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=135, Percent_Identity=66.6666666666667, Blast_Score=186, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 15687; Mature: 15556

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLKEFKAFAVKGNVVDMAVGIIIGAAFGKIVSSFVGDVIMPPLGLLIGGVDFSDLAVT
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEE
LRPAQGTAPAVLLAYGKFIQTVIDFIIVAFAIFMGVKAINRLKREEAKAPTLPPTPSKQE
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
ILLGEIRDLLKEQSKPAAPITVDPARTL
HHHHHHHHHHHHCCCCCCCEECCCCCCC
>Mature Secondary Structure 
SVLKEFKAFAVKGNVVDMAVGIIIGAAFGKIVSSFVGDVIMPPLGLLIGGVDFSDLAVT
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEE
LRPAQGTAPAVLLAYGKFIQTVIDFIIVAFAIFMGVKAINRLKREEAKAPTLPPTPSKQE
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
ILLGEIRDLLKEQSKPAAPITVDPARTL
HHHHHHHHHHHHCCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA