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The map label for this gene is opuBB [H]

Identifier: 66047477

GI number: 66047477

Start: 5067720

End: 5068373

Strand: Reverse

Name: opuBB [H]

Synonym: Psyr_4250

Alternate gene names: 66047477

Gene position: 5068373-5067720 (Counterclockwise)

Preceding gene: 66047478

Following gene: 66047476

Centisome position: 83.17

GC content: 61.93

Gene sequence:

>654_bases
ATGAGTTTCTTGAGCGCCTTCTCCCATCTGGACTGGGCACAGGTCCTGCAACTGACCTGGCAGCACATCACGCTGGTCGG
CATCGCCGTGACCCTGGCGATTGTCGTCGGTGTGCCGCTGGGCGTGTTGATGACGCGTTTTCCAGCGCTGGCGGGCCCCT
TGCAGGCCAGCGCCACGGTGCTGCTGACCGTGCCGTCGATTGCCCTGTTCGGCCTGCTGCTGCCGTTCTATTCCAAATTC
GGCCAGGGCCTTGGCCCGATGCCGGCGATTACCGCGGTGTTCCTCTACTCGCTGCTGCCGATCATGCGTAACACCTATCT
GGCGCTGACCGGGGTAGAACCGGGTATTCGCGAAGCCGCCAAAGGCATCGGCATGACCTTCGGCCAGCGTCTGCGCATGG
TCGAACTGCCCATCGCCGTGCCTGTGATCCTCGCCGGTGTGCGCACTGCCGTGGTGATGAATATCGGTGTCATGACCATC
GCCGCGACCATCGGCGCCGGTGGCCTGGGTGTACTCATTCTCGCTTCAATCAGCCGCAGCGATATGTCGATGCTGATCGT
CGGCGCGGTCCTGGTCAGCATTCTGGCCATCATCGCCGACCTGCTCCTGCAATGGCTGCAACGCACGCTGACTCCAAAAG
GATTACTGAAATGA

Upstream 100 bases:

>100_bases
ATCGACCTCAACGCTCGCGTCGACGTCGGCCATGAAAGCCCATCCGTTGTTGCCGCAGATTTTCTGCGCCAGCATCCATT
GAACTAAGGAAGGAGACGAT

Downstream 100 bases:

>100_bases
TCGAACTCCAGAACCTCTCCAAGACTTTCCAGAGCAACGGCAAAGAAGTGAAGGCTGTCGATTCGGTCAGCCTCACGGTC
AACGAAGGCGAAATCTGCGT

Product: binding-protein dependent transport system inner membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MSFLSAFSHLDWAQVLQLTWQHITLVGIAVTLAIVVGVPLGVLMTRFPALAGPLQASATVLLTVPSIALFGLLLPFYSKF
GQGLGPMPAITAVFLYSLLPIMRNTYLALTGVEPGIREAAKGIGMTFGQRLRMVELPIAVPVILAGVRTAVVMNIGVMTI
AATIGAGGLGVLILASISRSDMSMLIVGAVLVSILAIIADLLLQWLQRTLTPKGLLK

Sequences:

>Translated_217_residues
MSFLSAFSHLDWAQVLQLTWQHITLVGIAVTLAIVVGVPLGVLMTRFPALAGPLQASATVLLTVPSIALFGLLLPFYSKF
GQGLGPMPAITAVFLYSLLPIMRNTYLALTGVEPGIREAAKGIGMTFGQRLRMVELPIAVPVILAGVRTAVVMNIGVMTI
AATIGAGGLGVLILASISRSDMSMLIVGAVLVSILAIIADLLLQWLQRTLTPKGLLK
>Mature_216_residues
SFLSAFSHLDWAQVLQLTWQHITLVGIAVTLAIVVGVPLGVLMTRFPALAGPLQASATVLLTVPSIALFGLLLPFYSKFG
QGLGPMPAITAVFLYSLLPIMRNTYLALTGVEPGIREAAKGIGMTFGQRLRMVELPIAVPVILAGVRTAVVMNIGVMTIA
ATIGAGGLGVLILASISRSDMSMLIVGAVLVSILAIIADLLLQWLQRTLTPKGLLK

Specific function: Involved in a high affinity multicomponent binding- protein-dependent transport system for choline; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1174

COG function: function code E; ABC-type proline/glycine betaine transport systems, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788449, Length=192, Percent_Identity=42.7083333333333, Blast_Score=127, Evalue=4e-31,
Organism=Escherichia coli, GI1788451, Length=195, Percent_Identity=37.4358974358974, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1789033, Length=183, Percent_Identity=34.9726775956284, Blast_Score=93, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 22840; Mature: 22709

Theoretical pI: Translated: 10.90; Mature: 10.90

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFLSAFSHLDWAQVLQLTWQHITLVGIAVTLAIVVGVPLGVLMTRFPALAGPLQASATV
CCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCEE
LLTVPSIALFGLLLPFYSKFGQGLGPMPAITAVFLYSLLPIMRNTYLALTGVEPGIREAA
EEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHH
KGIGMTFGQRLRMVELPIAVPVILAGVRTAVVMNIGVMTIAATIGAGGLGVLILASISRS
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHH
DMSMLIVGAVLVSILAIIADLLLQWLQRTLTPKGLLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SFLSAFSHLDWAQVLQLTWQHITLVGIAVTLAIVVGVPLGVLMTRFPALAGPLQASATV
CHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCEE
LLTVPSIALFGLLLPFYSKFGQGLGPMPAITAVFLYSLLPIMRNTYLALTGVEPGIREAA
EEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHH
KGIGMTFGQRLRMVELPIAVPVILAGVRTAVVMNIGVMTIAATIGAGGLGVLILASISRS
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHH
DMSMLIVGAVLVSILAIIADLLLQWLQRTLTPKGLLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7592481; 10216873; 9384377 [H]