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The map label for this gene is lutR [H]

Identifier: 66047455

GI number: 66047455

Start: 5035648

End: 5036397

Strand: Reverse

Name: lutR [H]

Synonym: Psyr_4228

Alternate gene names: 66047455

Gene position: 5036397-5035648 (Counterclockwise)

Preceding gene: 66047456

Following gene: 66047454

Centisome position: 82.65

GC content: 62.4

Gene sequence:

>750_bases
ATGGAAAATCAAAGCGCTCTGCCCCGCGCCCGCAGAAAACACCGCAGCCTCGCGCAGGAGCTGGTGACAGAGCTGTCCGA
GCGTATTCGCAGCGGTCAGCTCAAGCGCGGCGACAAACTGCCCACCGAATCCGCAATCATGGAAGAGCAGGGCGTCAGCA
GGACGGTCGTGCGTGAGGCGATTTCGCGTCTGCAGGCCTCCGGTCTGGTCGAGACCCGTCACGGCATCGGCACCTTCGTG
CTCGATACGCCAAGCCCCAGTGGCTTCAGGATTGACCCGGCGACCATCGTCACCCTGCGCGATGTACTGGCGATTCTCGA
ATTGCGCATCAGCCTTGAAGTCGAGTCGGCAGGCCTGGCAGCCCAGCGGCGTAGTGATGAACAACTGGCAGCCATGCGTT
CGGCGCTGGACGCGCTCAATGAAAGTGCGGCACACGCCACCGATGCCGTGGCCTCGGACTTCCAGTTCCATCTGCAGATT
GCCTTGTCGACCGGCAATCGTTACTTCACTGACATCATGACCCACCTGGGCACCAGCATCATCCCGCGTACCCGGCTCAA
TTCCGCGCGTCTGGCACATGATGACCAGCAGCACTATATGAATCGGCTGAGCCGCGAGCACGAAGAAATCTTCGACGCCA
TCGCCCGCCAGGATTCCGATGCGGCCCGCGCCGCCATGCGCCTGCACCTGACCAACAGCCGCGAACGCCTGCGCCATGCG
CATGAAGAGGCTGAATCGCAGCGGGCTTGA

Upstream 100 bases:

>100_bases
GAGGTCGATGCTATAGGGGACACGGCGCTTTTGAGGGTGTGATCATACAACCGCTGAGCTATGATTAGCCCATGTTTAGC
AGTCCAGAGATCGATGTTGG

Downstream 100 bases:

>100_bases
GGTTTAAAAAGAGGACGCAGAGCGTCCGGAACGGCATGCCCACGCTGGAGCGTGGGCACGATAGGTGTTCTCCCTGACAT
CTATCGTTCCTCACGCTCCA

Product: transcriptional regulator GntR

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MENQSALPRARRKHRSLAQELVTELSERIRSGQLKRGDKLPTESAIMEEQGVSRTVVREAISRLQASGLVETRHGIGTFV
LDTPSPSGFRIDPATIVTLRDVLAILELRISLEVESAGLAAQRRSDEQLAAMRSALDALNESAAHATDAVASDFQFHLQI
ALSTGNRYFTDIMTHLGTSIIPRTRLNSARLAHDDQQHYMNRLSREHEEIFDAIARQDSDAARAAMRLHLTNSRERLRHA
HEEAESQRA

Sequences:

>Translated_249_residues
MENQSALPRARRKHRSLAQELVTELSERIRSGQLKRGDKLPTESAIMEEQGVSRTVVREAISRLQASGLVETRHGIGTFV
LDTPSPSGFRIDPATIVTLRDVLAILELRISLEVESAGLAAQRRSDEQLAAMRSALDALNESAAHATDAVASDFQFHLQI
ALSTGNRYFTDIMTHLGTSIIPRTRLNSARLAHDDQQHYMNRLSREHEEIFDAIARQDSDAARAAMRLHLTNSRERLRHA
HEEAESQRA
>Mature_249_residues
MENQSALPRARRKHRSLAQELVTELSERIRSGQLKRGDKLPTESAIMEEQGVSRTVVREAISRLQASGLVETRHGIGTFV
LDTPSPSGFRIDPATIVTLRDVLAILELRISLEVESAGLAAQRRSDEQLAAMRSALDALNESAAHATDAVASDFQFHLQI
ALSTGNRYFTDIMTHLGTSIIPRTRLNSARLAHDDQQHYMNRLSREHEEIFDAIARQDSDAARAAMRLHLTNSRERLRHA
HEEAESQRA

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082208, Length=223, Percent_Identity=31.390134529148, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1790780, Length=227, Percent_Identity=31.2775330396476, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1790032, Length=238, Percent_Identity=29.4117647058824, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789621, Length=250, Percent_Identity=30.4, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI48994955, Length=226, Percent_Identity=29.646017699115, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI48994961, Length=230, Percent_Identity=27.8260869565217, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789353, Length=231, Percent_Identity=27.7056277056277, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 27845; Mature: 27845

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENQSALPRARRKHRSLAQELVTELSERIRSGQLKRGDKLPTESAIMEEQGVSRTVVREA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCHHHHHHHH
ISRLQASGLVETRHGIGTFVLDTPSPSGFRIDPATIVTLRDVLAILELRISLEVESAGLA
HHHHHHHCCHHHHCCCEEEEEECCCCCCEEECHHHHHHHHHHHHHHHHHHHEEEHHHCHH
AQRRSDEQLAAMRSALDALNESAAHATDAVASDFQFHLQIALSTGNRYFTDIMTHLGTSI
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCHHHHHHHHHHCCHH
IPRTRLNSARLAHDDQQHYMNRLSREHEEIFDAIARQDSDAARAAMRLHLTNSRERLRHA
CCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHH
HEEAESQRA
HHHHHHCCC
>Mature Secondary Structure
MENQSALPRARRKHRSLAQELVTELSERIRSGQLKRGDKLPTESAIMEEQGVSRTVVREA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCHHHHHHHH
ISRLQASGLVETRHGIGTFVLDTPSPSGFRIDPATIVTLRDVLAILELRISLEVESAGLA
HHHHHHHCCHHHHCCCEEEEEECCCCCCEEECHHHHHHHHHHHHHHHHHHHEEEHHHCHH
AQRRSDEQLAAMRSALDALNESAAHATDAVASDFQFHLQIALSTGNRYFTDIMTHLGTSI
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCHHHHHHHHHHCCHH
IPRTRLNSARLAHDDQQHYMNRLSREHEEIFDAIARQDSDAARAAMRLHLTNSRERLRHA
CCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHH
HEEAESQRA
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]