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The map label for this gene is carA [H]

Identifier: 66047419

GI number: 66047419

Start: 4987054

End: 4988190

Strand: Reverse

Name: carA [H]

Synonym: Psyr_4192

Alternate gene names: 66047419

Gene position: 4988190-4987054 (Counterclockwise)

Preceding gene: 66047420

Following gene: 66047418

Centisome position: 81.86

GC content: 60.86

Gene sequence:

>1137_bases
TTGACTAAGCCAGCCATACTCGCCCTTGCTGATGGCAGCATTTTTCGCGGCGAAGCCATTGGAGCCGACGGTCAGACCGT
TGGTGAGGTGGTGTTCAACACCGCAATGACAGGCTATCAGGAAATTCTTACCGATCCTTCCTACGCCCAGCAGATCGTTA
CCCTGACTTACCCGCATATCGGCAACACCGGCACCACCCCTGAAGACGCAGAGTCGGATCGCGTCTGGTCCGCCGGCCTG
GTCATTCGCGACCTGCCTCTGGTAGCCAGCAACTGGCGTAACAAGCTGTCCCTCGGTGATTACCTGAAAGCCAACAACGT
CGTTGCCATCGCTGGCATCGATACGCGTCGCCTGACCCGCATCCTGCGTGAGAAAGGCGCCCAGAACGGCTGCATCCTGG
CCGGCGATAACATCACCGAAGAGGCAGCCATCGCCGCTGCCCGCGGCTTCCCGGGCCTCAAGGGCATGGACCTGGCCAAG
GAAGTCTGCACCAAGGACACCTACGAGTGGCGTTCCAGCGTCTGGGATCTGAAAACCGACAGCCATCCTGAAATCGCCGC
CTCCGAGCTGCCTTATCATGTGGTCGCCTACGATTATGGCGTCAAGGTCAACATCCTGCGCATGCTGGTCGAGCGCGGTT
GCCGCGTAACCGTGGTGCCAGCGCAAACGCCTGCCAGTGAAGTGCTCGCCTACAAGCCGGACGGCGTTTTCCTGTCCAAC
GGCCCAGGTGACCCGGAGCCGTGCGATTACGCCATCAAGGCGATCAGGGAAGTGCTGGAAACCGACATTCCGGTGTTCGG
CATCTGCCTTGGCCACCAGTTGCTGGCACTGGCCGCGGGTGCCAAGACCGTGAAGATGGGCCACGGTCACCACGGTGCCA
ACCACCCGGTCCAGGATCTGGACACCGGTGTGGTCATGATCACCAGCCAGAACCACGGTTTTGCGGTGGATGAGGCAACC
CTGCCGAGCAACGTTCGCGCCATTCACAAGTCGCTGTTCGACGGCACCCTGCAGGGTATCGAACTGACCGACAAGAGCGC
ATTCAGCTTCCAGGGTCACCCTGAAGCGAGTCCGGGCCCGAACGACGTCGCGCCTTTGTTCGATCGCTTTATCGAAGCCA
TGGCCAAGCGCCGCTGA

Upstream 100 bases:

>100_bases
CGCAGATGATTCCATAAGAAAGCGGGGTGACGTAACTACGTCATTCCGCTTTTTTGCAGCCTGCGATCGCCCTTTCAGGT
TTTATTTACGGGAGGTCTTC

Downstream 100 bases:

>100_bases
CCGGTTGCCTTGAGACTGTAATACGCAGGTGCCCAGGGCGGCCCCGGACTCAGTGTCCGGTCGCTGCCGCCCAGGCCACC
TCAGACAAATGTTCAAGACG

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MTKPAILALADGSIFRGEAIGADGQTVGEVVFNTAMTGYQEILTDPSYAQQIVTLTYPHIGNTGTTPEDAESDRVWSAGL
VIRDLPLVASNWRNKLSLGDYLKANNVVAIAGIDTRRLTRILREKGAQNGCILAGDNITEEAAIAAARGFPGLKGMDLAK
EVCTKDTYEWRSSVWDLKTDSHPEIAASELPYHVVAYDYGVKVNILRMLVERGCRVTVVPAQTPASEVLAYKPDGVFLSN
GPGDPEPCDYAIKAIREVLETDIPVFGICLGHQLLALAAGAKTVKMGHGHHGANHPVQDLDTGVVMITSQNHGFAVDEAT
LPSNVRAIHKSLFDGTLQGIELTDKSAFSFQGHPEASPGPNDVAPLFDRFIEAMAKRR

Sequences:

>Translated_378_residues
MTKPAILALADGSIFRGEAIGADGQTVGEVVFNTAMTGYQEILTDPSYAQQIVTLTYPHIGNTGTTPEDAESDRVWSAGL
VIRDLPLVASNWRNKLSLGDYLKANNVVAIAGIDTRRLTRILREKGAQNGCILAGDNITEEAAIAAARGFPGLKGMDLAK
EVCTKDTYEWRSSVWDLKTDSHPEIAASELPYHVVAYDYGVKVNILRMLVERGCRVTVVPAQTPASEVLAYKPDGVFLSN
GPGDPEPCDYAIKAIREVLETDIPVFGICLGHQLLALAAGAKTVKMGHGHHGANHPVQDLDTGVVMITSQNHGFAVDEAT
LPSNVRAIHKSLFDGTLQGIELTDKSAFSFQGHPEASPGPNDVAPLFDRFIEAMAKRR
>Mature_377_residues
TKPAILALADGSIFRGEAIGADGQTVGEVVFNTAMTGYQEILTDPSYAQQIVTLTYPHIGNTGTTPEDAESDRVWSAGLV
IRDLPLVASNWRNKLSLGDYLKANNVVAIAGIDTRRLTRILREKGAQNGCILAGDNITEEAAIAAARGFPGLKGMDLAKE
VCTKDTYEWRSSVWDLKTDSHPEIAASELPYHVVAYDYGVKVNILRMLVERGCRVTVVPAQTPASEVLAYKPDGVFLSNG
PGDPEPCDYAIKAIREVLETDIPVFGICLGHQLLALAAGAKTVKMGHGHHGANHPVQDLDTGVVMITSQNHGFAVDEATL
PSNVRAIHKSLFDGTLQGIELTDKSAFSFQGHPEASPGPNDVAPLFDRFIEAMAKRR

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=383, Percent_Identity=39.1644908616188, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI21361331, Length=385, Percent_Identity=36.1038961038961, Blast_Score=214, Evalue=8e-56,
Organism=Homo sapiens, GI169790915, Length=385, Percent_Identity=36.1038961038961, Blast_Score=214, Evalue=8e-56,
Organism=Escherichia coli, GI1786215, Length=379, Percent_Identity=70.4485488126649, Blast_Score=553, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI193204318, Length=388, Percent_Identity=39.1752577319588, Blast_Score=248, Evalue=5e-66,
Organism=Saccharomyces cerevisiae, GI6324878, Length=391, Percent_Identity=38.3631713554987, Blast_Score=235, Evalue=7e-63,
Organism=Saccharomyces cerevisiae, GI6322331, Length=392, Percent_Identity=36.734693877551, Blast_Score=224, Evalue=2e-59,
Organism=Drosophila melanogaster, GI45555749, Length=395, Percent_Identity=38.7341772151899, Blast_Score=229, Evalue=3e-60,
Organism=Drosophila melanogaster, GI24642586, Length=395, Percent_Identity=38.7341772151899, Blast_Score=228, Evalue=4e-60,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 40672; Mature: 40540

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPAILALADGSIFRGEAIGADGQTVGEVVFNTAMTGYQEILTDPSYAQQIVTLTYPHI
CCCCEEEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHEEEEEECCCC
GNTGTTPEDAESDRVWSAGLVIRDLPLVASNWRNKLSLGDYLKANNVVAIAGIDTRRLTR
CCCCCCCCCCCCCCEEECCEEEECCCHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHHH
ILREKGAQNGCILAGDNITEEAAIAAARGFPGLKGMDLAKEVCTKDTYEWRSSVWDLKTD
HHHHCCCCCCEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCC
SHPEIAASELPYHVVAYDYGVKVNILRMLVERGCRVTVVPAQTPASEVLAYKPDGVFLSN
CCCCCHHHHCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCHHHHEEECCCCEEECC
GPGDPEPCDYAIKAIREVLETDIPVFGICLGHQLLALAAGAKTVKMGHGHHGANHPVQDL
CCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHC
DTGVVMITSQNHGFAVDEATLPSNVRAIHKSLFDGTLQGIELTDKSAFSFQGHPEASPGP
CCCEEEEEECCCCEEECCCCCCHHHHHHHHHHHHCCCCCEEECCCCCEEECCCCCCCCCC
NDVAPLFDRFIEAMAKRR
CHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKPAILALADGSIFRGEAIGADGQTVGEVVFNTAMTGYQEILTDPSYAQQIVTLTYPHI
CCCEEEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHEEEEEECCCC
GNTGTTPEDAESDRVWSAGLVIRDLPLVASNWRNKLSLGDYLKANNVVAIAGIDTRRLTR
CCCCCCCCCCCCCCEEECCEEEECCCHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHHH
ILREKGAQNGCILAGDNITEEAAIAAARGFPGLKGMDLAKEVCTKDTYEWRSSVWDLKTD
HHHHCCCCCCEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCC
SHPEIAASELPYHVVAYDYGVKVNILRMLVERGCRVTVVPAQTPASEVLAYKPDGVFLSN
CCCCCHHHHCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCHHHHEEECCCCEEECC
GPGDPEPCDYAIKAIREVLETDIPVFGICLGHQLLALAAGAKTVKMGHGHHGANHPVQDL
CCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHC
DTGVVMITSQNHGFAVDEATLPSNVRAIHKSLFDGTLQGIELTDKSAFSFQGHPEASPGP
CCCEEEEEECCCCEEECCCCCCHHHHHHHHHHHHCCCCCEEECCCCCEEECCCCCCCCCC
NDVAPLFDRFIEAMAKRR
CHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]