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The map label for this gene is iciA [H]

Identifier: 66047284

GI number: 66047284

Start: 4839666

End: 4840565

Strand: Reverse

Name: iciA [H]

Synonym: Psyr_4057

Alternate gene names: 66047284

Gene position: 4840565-4839666 (Counterclockwise)

Preceding gene: 66047289

Following gene: 66047283

Centisome position: 79.44

GC content: 62.89

Gene sequence:

>900_bases
ATGTTCGACTACAAGCTGTTGTCCGCGCTCGCTGCAGTCATCGAGCAGGCCGGGTTCGAACGTGCGGCCCAGGTGCTGGG
GCTGTCGCAGTCAGCGGTCTCGCAACGGATCAAATTGCTGGAGGCGCGCATTGGGCAGCCGGTGCTGGTCCGCGCTACGC
CGCCGAGCCCGACCGACATCGGCCGACGCCTGCTCAATCACGTGCAGCAGGTCCGCTTGCTGGAGCGCGATCTGCAAAGT
CAGGTGCCGGCACTGGACGAGGAGGGCATGCCCGAGCGTTTGCGGATCGCCTTGAACGCCGACAGTCTGGCAACGTGGTG
GGCACCGGCCATTGGTGACTTCTGCACGCAGCATCGTCTGTTGCTGGACCTGGTGGTCGAAGATCAGGACGTCGGGCTCA
AACGCATGCGGGCTGGCGAAGTCGCAGCGTGTCTGTGTGGCAGTGAGCGCCCGGTGGCCGGGGCGCGCAGTCAGTTGCTG
GGGGCGATGCGCTATCGGGCGCTGGCCAGCCCGGCATTCATCGCGCGCCATTTTGCGGACGGTGTCAGCCCCGAAAAACT
GGTCAGATCAGCGGCGCTCGTTTACGGGCCGGATGATCTTTTGCAGCATCGTTATCTGGCATTGCTTGGTGTGCAGGACG
GTTTCGAGCATCATTTGTGCCCTTCTTCCGAGGGTTTCATCCGTATGATCGAAGCCGGAATGGGCTGGGGGCTGGCGCCC
GAACTGCAAGTGCGCGAGCAATTGCGCAGCGGCACGCTGCTTGAACTGCTGCCGGATCGCTGTATTGATGTGCCGTTGTA
CTGGCACCATTGGCGCAACGGTGGTCAGTTGCTGACACGTCTCACCGGGCATCTGGCACAACATGCTGCCCACTGGCTGG
TGCCGTTGAGCGATGAATGA

Upstream 100 bases:

>100_bases
CACATAAATACGTTCCCATCGAGCAGATTCAGGTGGCGACTTTCAATGTTGAAGCGATATAAGTAAAACCAATAATCATC
ATCTCTCATTAGGGAAACTG

Downstream 100 bases:

>100_bases
TCGTCATTACGATCATGTGTAATCAATGCGTGTCGGGTTACCCCTGTCACGCAGACGCGTTAATGGAGCGGTAAATGAAA
ATTCTGGTCACCGGCGCAAG

Product: chromosome replication initiation inhibitor protein

Products: NA

Alternate protein names: OriC replication inhibitor [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MFDYKLLSALAAVIEQAGFERAAQVLGLSQSAVSQRIKLLEARIGQPVLVRATPPSPTDIGRRLLNHVQQVRLLERDLQS
QVPALDEEGMPERLRIALNADSLATWWAPAIGDFCTQHRLLLDLVVEDQDVGLKRMRAGEVAACLCGSERPVAGARSQLL
GAMRYRALASPAFIARHFADGVSPEKLVRSAALVYGPDDLLQHRYLALLGVQDGFEHHLCPSSEGFIRMIEAGMGWGLAP
ELQVREQLRSGTLLELLPDRCIDVPLYWHHWRNGGQLLTRLTGHLAQHAAHWLVPLSDE

Sequences:

>Translated_299_residues
MFDYKLLSALAAVIEQAGFERAAQVLGLSQSAVSQRIKLLEARIGQPVLVRATPPSPTDIGRRLLNHVQQVRLLERDLQS
QVPALDEEGMPERLRIALNADSLATWWAPAIGDFCTQHRLLLDLVVEDQDVGLKRMRAGEVAACLCGSERPVAGARSQLL
GAMRYRALASPAFIARHFADGVSPEKLVRSAALVYGPDDLLQHRYLALLGVQDGFEHHLCPSSEGFIRMIEAGMGWGLAP
ELQVREQLRSGTLLELLPDRCIDVPLYWHHWRNGGQLLTRLTGHLAQHAAHWLVPLSDE
>Mature_299_residues
MFDYKLLSALAAVIEQAGFERAAQVLGLSQSAVSQRIKLLEARIGQPVLVRATPPSPTDIGRRLLNHVQQVRLLERDLQS
QVPALDEEGMPERLRIALNADSLATWWAPAIGDFCTQHRLLLDLVVEDQDVGLKRMRAGEVAACLCGSERPVAGARSQLL
GAMRYRALASPAFIARHFADGVSPEKLVRSAALVYGPDDLLQHRYLALLGVQDGFEHHLCPSSEGFIRMIEAGMGWGLAP
ELQVREQLRSGTLLELLPDRCIDVPLYWHHWRNGGQLLTRLTGHLAQHAAHWLVPLSDE

Specific function: Specific inhibitor of chromosomal initiation of replication in vitro. Binds the three 13-mers in the origin (oriC) to block initiation of replication [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789283, Length=295, Percent_Identity=35.5932203389831, Blast_Score=166, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR017685
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33111; Mature: 33111

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDYKLLSALAAVIEQAGFERAAQVLGLSQSAVSQRIKLLEARIGQPVLVRATPPSPTDI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHH
GRRLLNHVQQVRLLERDLQSQVPALDEEGMPERLRIALNADSLATWWAPAIGDFCTQHRL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHH
LLDLVVEDQDVGLKRMRAGEVAACLCGSERPVAGARSQLLGAMRYRALASPAFIARHFAD
HHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHC
GVSPEKLVRSAALVYGPDDLLQHRYLALLGVQDGFEHHLCPSSEGFIRMIEAGMGWGLAP
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCC
ELQVREQLRSGTLLELLPDRCIDVPLYWHHWRNGGQLLTRLTGHLAQHAAHWLVPLSDE
HHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCEECCCC
>Mature Secondary Structure
MFDYKLLSALAAVIEQAGFERAAQVLGLSQSAVSQRIKLLEARIGQPVLVRATPPSPTDI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHH
GRRLLNHVQQVRLLERDLQSQVPALDEEGMPERLRIALNADSLATWWAPAIGDFCTQHRL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHH
LLDLVVEDQDVGLKRMRAGEVAACLCGSERPVAGARSQLLGAMRYRALASPAFIARHFAD
HHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHC
GVSPEKLVRSAALVYGPDDLLQHRYLALLGVQDGFEHHLCPSSEGFIRMIEAGMGWGLAP
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCC
ELQVREQLRSGTLLELLPDRCIDVPLYWHHWRNGGQLLTRLTGHLAQHAAHWLVPLSDE
HHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA