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The map label for this gene is relE [H]

Identifier: 66047060

GI number: 66047060

Start: 4569228

End: 4569518

Strand: Direct

Name: relE [H]

Synonym: Psyr_3833

Alternate gene names: 66047060

Gene position: 4569228-4569518 (Clockwise)

Preceding gene: 66047059

Following gene: 66047065

Centisome position: 74.98

GC content: 51.2

Gene sequence:

>291_bases
ATGACCTTTAAGCTTGAATTTCTGCCCTCGGCCTTGAAGGAGTGGGAAAAACTGGGCCATACCATCCGTGTTCAGCTCAA
GAAAAAGCTTCTCGAACGACTCGGACTACCAAGAATTCCAGGCGATGCGCTTCATGGCATGCCAGATCATTACAAAATCA
AACTGCGCAGTGCAGGATACCGACTCGTCTATCGAGTCGAGGAAGATAGGGTAGTGGTAACCGTCGTTGCGGTAGGCAAG
CGCGAGCGCGGGAATATTTATGATTCGGCCAAAGGCAGGCTTGGCCCTTGA

Upstream 100 bases:

>100_bases
GCCGAGGTGTTTGAAGCAATGATGGAGAGGCTTGATGACCTTGATCTGGCCGAGCTGATCAAATCTCGCCAACATGAAAA
GCCGGTGTCGGTAGATATAG

Downstream 100 bases:

>100_bases
GGTGCAGTGCATCTGCATTACACATCGGCATCCTGCAACACGCTCGGACAGGACGCAGAGCGTCAGGAAAGGCATTCACA
TGCGGAGCGTGAGGAACGAG

Product: plasmid stabilization system protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 96; Mature: 95

Protein sequence:

>96_residues
MTFKLEFLPSALKEWEKLGHTIRVQLKKKLLERLGLPRIPGDALHGMPDHYKIKLRSAGYRLVYRVEEDRVVVTVVAVGK
RERGNIYDSAKGRLGP

Sequences:

>Translated_96_residues
MTFKLEFLPSALKEWEKLGHTIRVQLKKKLLERLGLPRIPGDALHGMPDHYKIKLRSAGYRLVYRVEEDRVVVTVVAVGK
RERGNIYDSAKGRLGP
>Mature_95_residues
TFKLEFLPSALKEWEKLGHTIRVQLKKKLLERLGLPRIPGDALHGMPDHYKIKLRSAGYRLVYRVEEDRVVVTVVAVGKR
ERGNIYDSAKGRLGP

Specific function: Inhibits translation during amino acid starvation. Acts by cleaving mRNA with high codon specificity in the ribosomal A site. UGA is cleaved with fast rate while UAA and UGA are cleaved with intermediate and slow rate, respectively. mRNA cleavage can also

COG id: COG2026

COG function: function code JD; Cytotoxic translational repressor of toxin-antitoxin stability system

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787846, Length=94, Percent_Identity=59.5744680851064, Blast_Score=112, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007712
- InterPro:   IPR012753 [H]

Pfam domain/function: PF05016 Plasmid_stabil [H]

EC number: NA

Molecular weight: Translated: 11010; Mature: 10879

Theoretical pI: Translated: 10.73; Mature: 10.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFKLEFLPSALKEWEKLGHTIRVQLKKKLLERLGLPRIPGDALHGMPDHYKIKLRSAGY
CEEEHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCCCHHHCCCCCCEEEEEECCCE
RLVYRVEEDRVVVTVVAVGKRERGNIYDSAKGRLGP
EEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TFKLEFLPSALKEWEKLGHTIRVQLKKKLLERLGLPRIPGDALHGMPDHYKIKLRSAGY
EEEHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCCCHHHCCCCCCEEEEEECCCE
RLVYRVEEDRVVVTVVAVGKRERGNIYDSAKGRLGP
EEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2990907; 9097039; 9278503; 9767574; 12526800 [H]