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The map label for this gene is opdE [H]

Identifier: 66047028

GI number: 66047028

Start: 4516878

End: 4518140

Strand: Reverse

Name: opdE [H]

Synonym: Psyr_3800

Alternate gene names: 66047028

Gene position: 4518140-4516878 (Counterclockwise)

Preceding gene: 66047030

Following gene: 66047027

Centisome position: 74.14

GC content: 63.5

Gene sequence:

>1263_bases
ATGCAAGCATTTGATTCAACTCTGAAGCAGTCCGAAATAACTACTCATCCGCCCGCATGGGGCGCGGTGTTTTCGATGGC
GCTGTGTGTGGTGGTGCTGATTGCGTCGGAGTTCATGCCGGTCAGCCTGCTGACACCCATCGCGCAGGATCTGGGCATCA
GTCAGGGGCAGGCCGGGCAGGCGATTTCGATCTCCGGCTTTTTTGCCGTGCTGACCAGTCTGCTGAACACGCTGCTGACC
GGGCATCTGGATCGCAAGCCTGTCCTGCTGGGCTTCAGCCTGTTGTTGCTGGTGTCTGGCGTGATCGTCACGCTGGCCTC
CAACGGCTGGTTGTTCATGACGGGCCGTGCATTGCTCGGGATCGCCATTGGCGGGTTCTGGTCGATGTCGACCGCTACCG
TGATGAAGCTGGTGCCGAAGCAATCGGTCGCCAAGGGCCTGGCATTGATCAACGGCGGCAATGCGCTGGCGGCGACGGTC
GCCGCGCCATTGGGCAGCTTCATGGGCCAATACATCGGCTGGCGCGGGGCGTTTTTCCTGGTCATCCCGCTCGCGGCGCT
GGCGTTTGCCTGGCAGTGGCTGAGCCTGCCGGCCATGCGCAGTCAGCCGAACAACCGGGCACGCAATCCGTTCCGCCTGC
TGCGTAACCCGCAAGTGGCCGTCGGCATGAGCGCGATCCTGTTGCTGTTCATGGGCCAGTTCGCCGTCTTCACCTACCTG
CGCCCGTTTCTGGAAGAGATCACCGGGGTCAGCATCAACACGTTGTCCCTGATGCTTTTGGCGCTGGGGGCCAGCGGGCT
GGTCGGCACCTACCTGATCGGCAGGCTGCTGCACAAAGGCCTGTATGCCTGCCTGATCGCCATTCCGCTGCTGATGGCGG
TGCTGGCCGTGGCGCTCACTGGCCTGGGTCATTCACCTCAGTCCGTCGCCATTGTGCTTGCCGTCTGGGGCCTGATTGCC
ACGCCTGCACCGGTCGCCTGGGGCCTGTGGTTGAGCCGCACGCTGCCCGACGATGCCGAGGCAGGCGGCGGCCTGATGGT
GGCGACCATTCAATTGGCGATCACCGCCGGGGCCGGCATCGGCGGCGCGCTGTTCGACAGCCTCGGTTGGTGGAGCCCGT
TCGCATTCGGTGGCGTGTTACTGGCGTGTTCGGCCGCGCTGGCCTGGACGGCACGCGACAACGCGTTACTGCAGGAAAAA
AACACCGGTCAGGGCCGGGCAGATAAACGCGGGTCAATTTCTCAACGAGGTAACACGCCATGA

Upstream 100 bases:

>100_bases
ATCCTTCTCACAAGCGCGTTTAAATTGTGGTGTCTAATCATCCTAATACCTTTGCCCTAACCTGTGCCGACTAATTTTTA
ACGTCGTCAGGTCAGGACGC

Downstream 100 bases:

>100_bases
AAAAGATTCTTTTGATGCTTGGGCTTTTAATCAGCTCATACGCTGCGACAGGAGCAGACATGTCTCACGGTGCCGATAAT
TTCTACACAAGCGACAAGGT

Product: major facilitator transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MQAFDSTLKQSEITTHPPAWGAVFSMALCVVVLIASEFMPVSLLTPIAQDLGISQGQAGQAISISGFFAVLTSLLNTLLT
GHLDRKPVLLGFSLLLLVSGVIVTLASNGWLFMTGRALLGIAIGGFWSMSTATVMKLVPKQSVAKGLALINGGNALAATV
AAPLGSFMGQYIGWRGAFFLVIPLAALAFAWQWLSLPAMRSQPNNRARNPFRLLRNPQVAVGMSAILLLFMGQFAVFTYL
RPFLEEITGVSINTLSLMLLALGASGLVGTYLIGRLLHKGLYACLIAIPLLMAVLAVALTGLGHSPQSVAIVLAVWGLIA
TPAPVAWGLWLSRTLPDDAEAGGGLMVATIQLAITAGAGIGGALFDSLGWWSPFAFGGVLLACSAALAWTARDNALLQEK
NTGQGRADKRGSISQRGNTP

Sequences:

>Translated_420_residues
MQAFDSTLKQSEITTHPPAWGAVFSMALCVVVLIASEFMPVSLLTPIAQDLGISQGQAGQAISISGFFAVLTSLLNTLLT
GHLDRKPVLLGFSLLLLVSGVIVTLASNGWLFMTGRALLGIAIGGFWSMSTATVMKLVPKQSVAKGLALINGGNALAATV
AAPLGSFMGQYIGWRGAFFLVIPLAALAFAWQWLSLPAMRSQPNNRARNPFRLLRNPQVAVGMSAILLLFMGQFAVFTYL
RPFLEEITGVSINTLSLMLLALGASGLVGTYLIGRLLHKGLYACLIAIPLLMAVLAVALTGLGHSPQSVAIVLAVWGLIA
TPAPVAWGLWLSRTLPDDAEAGGGLMVATIQLAITAGAGIGGALFDSLGWWSPFAFGGVLLACSAALAWTARDNALLQEK
NTGQGRADKRGSISQRGNTP
>Mature_420_residues
MQAFDSTLKQSEITTHPPAWGAVFSMALCVVVLIASEFMPVSLLTPIAQDLGISQGQAGQAISISGFFAVLTSLLNTLLT
GHLDRKPVLLGFSLLLLVSGVIVTLASNGWLFMTGRALLGIAIGGFWSMSTATVMKLVPKQSVAKGLALINGGNALAATV
AAPLGSFMGQYIGWRGAFFLVIPLAALAFAWQWLSLPAMRSQPNNRARNPFRLLRNPQVAVGMSAILLLFMGQFAVFTYL
RPFLEEITGVSINTLSLMLLALGASGLVGTYLIGRLLHKGLYACLIAIPLLMAVLAVALTGLGHSPQSVAIVLAVWGLIA
TPAPVAWGLWLSRTLPDDAEAGGGLMVATIQLAITAGAGIGGALFDSLGWWSPFAFGGVLLACSAALAWTARDNALLQEK
NTGQGRADKRGSISQRGNTP

Specific function: Regulates the expression of oprD which encodes the imipenem-specific porin [H]

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To B.subtilis ywfA [H]

Homologues:

Organism=Escherichia coli, GI145693198, Length=387, Percent_Identity=42.3772609819121, Blast_Score=295, Evalue=3e-81,
Organism=Escherichia coli, GI1787808, Length=243, Percent_Identity=26.7489711934156, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787947, Length=285, Percent_Identity=27.719298245614, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1786595, Length=396, Percent_Identity=26.2626262626263, Blast_Score=96, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43981; Mature: 43981

Theoretical pI: Translated: 10.40; Mature: 10.40

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAFDSTLKQSEITTHPPAWGAVFSMALCVVVLIASEFMPVSLLTPIAQDLGISQGQAGQ
CCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCC
AISISGFFAVLTSLLNTLLTGHLDRKPVLLGFSLLLLVSGVIVTLASNGWLFMTGRALLG
EEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHH
IAIGGFWSMSTATVMKLVPKQSVAKGLALINGGNALAATVAAPLGSFMGQYIGWRGAFFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHCHHHHH
VIPLAALAFAWQWLSLPAMRSQPNNRARNPFRLLRNPQVAVGMSAILLLFMGQFAVFTYL
HHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
RPFLEEITGVSINTLSLMLLALGASGLVGTYLIGRLLHKGLYACLIAIPLLMAVLAVALT
HHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLGHSPQSVAIVLAVWGLIATPAPVAWGLWLSRTLPDDAEAGGGLMVATIQLAITAGAGI
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEEEHHCCCCCC
GGALFDSLGWWSPFAFGGVLLACSAALAWTARDNALLQEKNTGQGRADKRGSISQRGNTP
CHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQAFDSTLKQSEITTHPPAWGAVFSMALCVVVLIASEFMPVSLLTPIAQDLGISQGQAGQ
CCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCC
AISISGFFAVLTSLLNTLLTGHLDRKPVLLGFSLLLLVSGVIVTLASNGWLFMTGRALLG
EEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHH
IAIGGFWSMSTATVMKLVPKQSVAKGLALINGGNALAATVAAPLGSFMGQYIGWRGAFFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHCHHHHH
VIPLAALAFAWQWLSLPAMRSQPNNRARNPFRLLRNPQVAVGMSAILLLFMGQFAVFTYL
HHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
RPFLEEITGVSINTLSLMLLALGASGLVGTYLIGRLLHKGLYACLIAIPLLMAVLAVALT
HHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLGHSPQSVAIVLAVWGLIATPAPVAWGLWLSRTLPDDAEAGGGLMVATIQLAITAGAGI
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEEEHHCCCCCC
GGALFDSLGWWSPFAFGGVLLACSAALAWTARDNALLQEKNTGQGRADKRGSISQRGNTP
CHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1427017; 10984043 [H]