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The map label for this gene is def [H]

Identifier: 66047008

GI number: 66047008

Start: 4498007

End: 4498546

Strand: Reverse

Name: def [H]

Synonym: Psyr_3780

Alternate gene names: 66047008

Gene position: 4498546-4498007 (Counterclockwise)

Preceding gene: 66047009

Following gene: 66047006

Centisome position: 73.82

GC content: 60.56

Gene sequence:

>540_bases
ATGATCCACAAAATCCTGAAGATGGGCGATGAGCGCTTGTTGCGCATCGCCCCTCCGGTACCCGCAGAGATGTTCGGCAG
CAGCGAGCTCGATACGCTGATTGCCGACATGTTCGAAACCATGCACAGCGTCGGCGGTGTGGGGCTGGCGGCACCGCAGA
TTGGCATCGATCTGCAACTGGTGATTTTCGGTTTCGAGCGCAACGAGCGCTATCCCGAGGCCGAGGCGGTCCCGCAGACG
ATCCTGATCAACCCGCTGATTACCCCGCTGAGCCCGACGCTGGAAGAGGGCTGGGAAGGCTGCCTCTCAGTGCCCGGCCT
GCGCGGCATGGTGGACCGTTATCAGAGCATTCGTTACGAAGGCTTCGATCCTCAGGGCCAGCCCATCGAGCGTGTTGCCC
ACGGTTTTCACGCGCGGGTCGTTCAGCATGAATGCGACCACCTGATCGGTCGGCTTTACCCGTCGCGCATCACCGATTTC
AGCAAATTCGGCTTCATGGACGTGATGTTTCCGGACATGGACCCCAACGCCGACGAGTGA

Upstream 100 bases:

>100_bases
GTCTGGGCCGCGACCATTCGGTGCCGTTGCAGAAGGCGGACGAAGCCTGATCGTGTTGTGTGGCAAGATCGCTTTTTAAG
TCGGCAAGAGACACGCAGTC

Downstream 100 bases:

>100_bases
TCGTCGTGTTGTGCTGCGACGCCATCAGGTTTCGCGGCACAGTGCGATCAGCGGCTTGCTGCGGCTGTAACGCATGAACC
GTTCACCCAGGGCCTGTGGC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MIHKILKMGDERLLRIAPPVPAEMFGSSELDTLIADMFETMHSVGGVGLAAPQIGIDLQLVIFGFERNERYPEAEAVPQT
ILINPLITPLSPTLEEGWEGCLSVPGLRGMVDRYQSIRYEGFDPQGQPIERVAHGFHARVVQHECDHLIGRLYPSRITDF
SKFGFMDVMFPDMDPNADE

Sequences:

>Translated_179_residues
MIHKILKMGDERLLRIAPPVPAEMFGSSELDTLIADMFETMHSVGGVGLAAPQIGIDLQLVIFGFERNERYPEAEAVPQT
ILINPLITPLSPTLEEGWEGCLSVPGLRGMVDRYQSIRYEGFDPQGQPIERVAHGFHARVVQHECDHLIGRLYPSRITDF
SKFGFMDVMFPDMDPNADE
>Mature_179_residues
MIHKILKMGDERLLRIAPPVPAEMFGSSELDTLIADMFETMHSVGGVGLAAPQIGIDLQLVIFGFERNERYPEAEAVPQT
ILINPLITPLSPTLEEGWEGCLSVPGLRGMVDRYQSIRYEGFDPQGQPIERVAHGFHARVVQHECDHLIGRLYPSRITDF
SKFGFMDVMFPDMDPNADE

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=175, Percent_Identity=30.8571428571429, Blast_Score=87, Evalue=8e-18,
Organism=Escherichia coli, GI1789682, Length=153, Percent_Identity=36.6013071895425, Blast_Score=95, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24645726, Length=167, Percent_Identity=34.7305389221557, Blast_Score=97, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24645728, Length=167, Percent_Identity=34.1317365269461, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 20067; Mature: 20067

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHKILKMGDERLLRIAPPVPAEMFGSSELDTLIADMFETMHSVGGVGLAAPQIGIDLQL
CHHHHHHCCCHHEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
VIFGFERNERYPEAEAVPQTILINPLITPLSPTLEEGWEGCLSVPGLRGMVDRYQSIRYE
EEEEECCCCCCCCHHHCCCCEECCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCC
GFDPQGQPIERVAHGFHARVVQHECDHLIGRLYPSRITDFSKFGFMDVMFPDMDPNADE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCC
>Mature Secondary Structure
MIHKILKMGDERLLRIAPPVPAEMFGSSELDTLIADMFETMHSVGGVGLAAPQIGIDLQL
CHHHHHHCCCHHEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
VIFGFERNERYPEAEAVPQTILINPLITPLSPTLEEGWEGCLSVPGLRGMVDRYQSIRYE
EEEEECCCCCCCCHHHCCCCEECCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCC
GFDPQGQPIERVAHGFHARVVQHECDHLIGRLYPSRITDFSKFGFMDVMFPDMDPNADE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]