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The map label for this gene is asnB [H]

Identifier: 66046975

GI number: 66046975

Start: 4464461

End: 4466233

Strand: Direct

Name: asnB [H]

Synonym: Psyr_3747

Alternate gene names: 66046975

Gene position: 4464461-4466233 (Clockwise)

Preceding gene: 66046973

Following gene: 66046976

Centisome position: 73.26

GC content: 61.2

Gene sequence:

>1773_bases
ATGTGCGGATTAGCAGGTGAATTACGTTTCGACCATCAACCAGCGGACTTAGCCGCTGTTGAACGCATTACCCATGAGCT
GGCGCCTCGCGGCCCGGATGCGTGGGGGTTTCATAGCCAGGGGCCGGTTGCCCTTGGTCATCGTCGCCTGAAAATCATGG
ACCTGTCGGACGGCTCGGCGCAGCCGATGATCGACAGCAATCTGGGCCTGTCCCTGGCCTTCAACGGCGCGATCTACAAC
TTTCCTGAATTGCGTGCCGAGTTGGAAAGCCTTGGCTATCAGTTCCATTCCGGCGGTGACACCGAAGTGTTGCTCAAGGG
CTATCACGCCTGGGGCGCAGACATGCTGCCCAAGCTCAATGGCATGTTCGCCTTTGCCATCTGGGAACGTGACAGCAAGC
AGCTGTTCATCGCCCGTGACCGCCTCGGCGTCAAGCCGTTGTACCTGTCAAAAACCGACAAGCGCCTGCGCTTCGCGTCC
TCGCTGCCTGCCTTGCTCAAGGGCGGTGATATCAGCGGGATGCTTGATCCGGTGGCGCTCAACCATTACCTCAACTTCCA
TGCGGTAGTGCCTGCGCCGCGCACCCTGCTGGCCGGCGTCGAAAAACTTCCGCCAGCCAGCTGGATGCGTATCGACGCCA
GCGGCAAGGTCGAACAGAAAGTCTGGTGGACGCTGCCCTACGGCCCGCACGAAGACGAGAAAAACCTCACCCTCGAAGAC
TGGCGCGACCGCGTACTGGACAGCACGCGTGAAGCGGTAGCGATTCGTCAGCGTGCTGCAGTGGACGTCGGTGTGCTGCT
GTCCGGCGGCGTCGACTCGAGCATGCTGGTCGGTCTGTTGCGCGAAGTGGGCGTCGAGGACCTGTCGACCTTCTCTATCG
GTTTCCAGGATGCCGGTGGCGAGCGCGGTGACGAATTTCAGTATTCCGATCTGATCGCCAAGCATTACGGCACGCGCCAT
CACCAGTTGCGTATTCAGGAACGCGAGATCATCGAGCAGTTGCCTGCGGCGTTCCGCGCCATGAGCGAACCGATGGTCAG
CCACGACTGCATCGCCTTCTACCTGCTGTCGCGTGAAGTGTCAAAACACTGCAAGGTGGTACAGAGCGGCCAGGGCGCTG
ACGAACTGTTCGCCGGTTACCACTGGTACCCGCAAGTCGACGGCGCGAGCGATCCGGTTGCCGCCTACCGCGATGCATTC
GTCGACCGCAGCTACGCGGAATACGCCGAGACCGTGCAGCCGAAGTGGCGCACTGCCAACGACGCAGCCGGTGATTTCGT
TCGCGATCACTTTGCCCAGCCAGGCGCCAGCGCTGCTGTCGACAAGGCGTTGCGTCTGGACAGCACGGTGATGCTGGTCG
ATGACCCGGTCAAGCGTGTCGACAACATGACCATGGCCTGGGGCCTGGAAGCGCGCACGCCGTTCCTGGACTATCGTCTG
GTCGAACTGTCGGCTCGCATCCCGGGTAAATTCAAGCTGCCCGACGGTGGCAAACAGGTGCTGAAAGAAGCCGCACGCAT
GGTCATTCCTTCGGAAGTGATCGACCGCAAAAAAGGCTACTTCCCGGTGCCTGGCCTCAAACACTTGCAGGGCGACACGC
TTAACTGGGTGCGCGAGCTGCTGACCGACTCCAGTCAGGACCGCGGCCTGTTCAACCCTGCCATGGTCGACAAGTTGCTG
ACCAACCCCGAGGGCCAGCTCACGCCATTGCGCGGCTCCAAGCTGTGGCAACTGGCAGCGCTGAACCTGTGGCTCAGCGA
ACAGGGACTGTGA

Upstream 100 bases:

>100_bases
TATAACTTTTTGATCTAAAACTACCATGCCCTCACTGGGTCAGTTTCTGGGTGACTGCACTTTCTCAGTCACCTCCCCAA
CGGAAACCCGGTAAGGACTT

Downstream 100 bases:

>100_bases
TCGATGAAACAGAACGCGACGACTTATAGCCAGCGCCTGTTGCGCGGCCAGTCGCCCTCGTATGAACGTCTGCAGGCGCG
ACTCGCTGAAGACGGCAGCC

Product: asparagine synthase, glutamine-hydrolyzing

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 590; Mature: 590

Protein sequence:

>590_residues
MCGLAGELRFDHQPADLAAVERITHELAPRGPDAWGFHSQGPVALGHRRLKIMDLSDGSAQPMIDSNLGLSLAFNGAIYN
FPELRAELESLGYQFHSGGDTEVLLKGYHAWGADMLPKLNGMFAFAIWERDSKQLFIARDRLGVKPLYLSKTDKRLRFAS
SLPALLKGGDISGMLDPVALNHYLNFHAVVPAPRTLLAGVEKLPPASWMRIDASGKVEQKVWWTLPYGPHEDEKNLTLED
WRDRVLDSTREAVAIRQRAAVDVGVLLSGGVDSSMLVGLLREVGVEDLSTFSIGFQDAGGERGDEFQYSDLIAKHYGTRH
HQLRIQEREIIEQLPAAFRAMSEPMVSHDCIAFYLLSREVSKHCKVVQSGQGADELFAGYHWYPQVDGASDPVAAYRDAF
VDRSYAEYAETVQPKWRTANDAAGDFVRDHFAQPGASAAVDKALRLDSTVMLVDDPVKRVDNMTMAWGLEARTPFLDYRL
VELSARIPGKFKLPDGGKQVLKEAARMVIPSEVIDRKKGYFPVPGLKHLQGDTLNWVRELLTDSSQDRGLFNPAMVDKLL
TNPEGQLTPLRGSKLWQLAALNLWLSEQGL

Sequences:

>Translated_590_residues
MCGLAGELRFDHQPADLAAVERITHELAPRGPDAWGFHSQGPVALGHRRLKIMDLSDGSAQPMIDSNLGLSLAFNGAIYN
FPELRAELESLGYQFHSGGDTEVLLKGYHAWGADMLPKLNGMFAFAIWERDSKQLFIARDRLGVKPLYLSKTDKRLRFAS
SLPALLKGGDISGMLDPVALNHYLNFHAVVPAPRTLLAGVEKLPPASWMRIDASGKVEQKVWWTLPYGPHEDEKNLTLED
WRDRVLDSTREAVAIRQRAAVDVGVLLSGGVDSSMLVGLLREVGVEDLSTFSIGFQDAGGERGDEFQYSDLIAKHYGTRH
HQLRIQEREIIEQLPAAFRAMSEPMVSHDCIAFYLLSREVSKHCKVVQSGQGADELFAGYHWYPQVDGASDPVAAYRDAF
VDRSYAEYAETVQPKWRTANDAAGDFVRDHFAQPGASAAVDKALRLDSTVMLVDDPVKRVDNMTMAWGLEARTPFLDYRL
VELSARIPGKFKLPDGGKQVLKEAARMVIPSEVIDRKKGYFPVPGLKHLQGDTLNWVRELLTDSSQDRGLFNPAMVDKLL
TNPEGQLTPLRGSKLWQLAALNLWLSEQGL
>Mature_590_residues
MCGLAGELRFDHQPADLAAVERITHELAPRGPDAWGFHSQGPVALGHRRLKIMDLSDGSAQPMIDSNLGLSLAFNGAIYN
FPELRAELESLGYQFHSGGDTEVLLKGYHAWGADMLPKLNGMFAFAIWERDSKQLFIARDRLGVKPLYLSKTDKRLRFAS
SLPALLKGGDISGMLDPVALNHYLNFHAVVPAPRTLLAGVEKLPPASWMRIDASGKVEQKVWWTLPYGPHEDEKNLTLED
WRDRVLDSTREAVAIRQRAAVDVGVLLSGGVDSSMLVGLLREVGVEDLSTFSIGFQDAGGERGDEFQYSDLIAKHYGTRH
HQLRIQEREIIEQLPAAFRAMSEPMVSHDCIAFYLLSREVSKHCKVVQSGQGADELFAGYHWYPQVDGASDPVAAYRDAF
VDRSYAEYAETVQPKWRTANDAAGDFVRDHFAQPGASAAVDKALRLDSTVMLVDDPVKRVDNMTMAWGLEARTPFLDYRL
VELSARIPGKFKLPDGGKQVLKEAARMVIPSEVIDRKKGYFPVPGLKHLQGDTLNWVRELLTDSSQDRGLFNPAMVDKLL
TNPEGQLTPLRGSKLWQLAALNLWLSEQGL

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786889, Length=563, Percent_Identity=25.9325044404973, Blast_Score=149, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6325403, Length=589, Percent_Identity=25.6366723259762, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321563, Length=647, Percent_Identity=24.8840803709428, Blast_Score=121, Evalue=3e-28,
Organism=Drosophila melanogaster, GI45553209, Length=569, Percent_Identity=25.8347978910369, Blast_Score=126, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 65596; Mature: 65596

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGLAGELRFDHQPADLAAVERITHELAPRGPDAWGFHSQGPVALGHRRLKIMDLSDGSA
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCEEEEEECCCCCC
QPMIDSNLGLSLAFNGAIYNFPELRAELESLGYQFHSGGDTEVLLKGYHAWGADMLPKLN
CCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCEEECCCCCCEEEEEECCCCCCHHHHHHC
GMFAFAIWERDSKQLFIARDRLGVKPLYLSKTDKRLRFASSLPALLKGGDISGMLDPVAL
CEEEEEEEECCCCEEEEEECCCCCCEEEECCCHHHHHHHHCCHHHHCCCCCCCCCCHHHH
NHYLNFHAVVPAPRTLLAGVEKLPPASWMRIDASGKVEQKVWWTLPYGPHEDEKNLTLED
HHHCCEEEECCCCHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEECCCCCCCCCCCCHHH
WRDRVLDSTREAVAIRQRAAVDVGVLLSGGVDSSMLVGLLREVGVEDLSTFSIGFQDAGG
HHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHCHHHHHHEEECCCCCCC
ERGDEFQYSDLIAKHYGTRHHQLRIQEREIIEQLPAAFRAMSEPMVSHDCIAFYLLSREV
CCCCCCHHHHHHHHHCCCCHHHEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
SKHCKVVQSGQGADELFAGYHWYPQVDGASDPVAAYRDAFVDRSYAEYAETVQPKWRTAN
HHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCC
DAAGDFVRDHFAQPGASAAVDKALRLDSTVMLVDDPVKRVDNMTMAWGLEARTPFLDYRL
CHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEECCHHHHHCCCEEEECCCCCCCCCCEEE
VELSARIPGKFKLPDGGKQVLKEAARMVIPSEVIDRKKGYFPVPGLKHLQGDTLNWVREL
EEECCCCCCCEECCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCHHCCCCHHHHHHHH
LTDSSQDRGLFNPAMVDKLLTNPEGQLTPLRGSKLWQLAALNLWLSEQGL
HCCCCCCCCCCCHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MCGLAGELRFDHQPADLAAVERITHELAPRGPDAWGFHSQGPVALGHRRLKIMDLSDGSA
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCEEEEEECCCCCC
QPMIDSNLGLSLAFNGAIYNFPELRAELESLGYQFHSGGDTEVLLKGYHAWGADMLPKLN
CCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCEEECCCCCCEEEEEECCCCCCHHHHHHC
GMFAFAIWERDSKQLFIARDRLGVKPLYLSKTDKRLRFASSLPALLKGGDISGMLDPVAL
CEEEEEEEECCCCEEEEEECCCCCCEEEECCCHHHHHHHHCCHHHHCCCCCCCCCCHHHH
NHYLNFHAVVPAPRTLLAGVEKLPPASWMRIDASGKVEQKVWWTLPYGPHEDEKNLTLED
HHHCCEEEECCCCHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEECCCCCCCCCCCCHHH
WRDRVLDSTREAVAIRQRAAVDVGVLLSGGVDSSMLVGLLREVGVEDLSTFSIGFQDAGG
HHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHCHHHHHHEEECCCCCCC
ERGDEFQYSDLIAKHYGTRHHQLRIQEREIIEQLPAAFRAMSEPMVSHDCIAFYLLSREV
CCCCCCHHHHHHHHHCCCCHHHEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
SKHCKVVQSGQGADELFAGYHWYPQVDGASDPVAAYRDAFVDRSYAEYAETVQPKWRTAN
HHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCC
DAAGDFVRDHFAQPGASAAVDKALRLDSTVMLVDDPVKRVDNMTMAWGLEARTPFLDYRL
CHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEECCHHHHHCCCEEEECCCCCCCCCCEEE
VELSARIPGKFKLPDGGKQVLKEAARMVIPSEVIDRKKGYFPVPGLKHLQGDTLNWVREL
EEECCCCCCCEECCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCHHCCCCHHHHHHHH
LTDSSQDRGLFNPAMVDKLLTNPEGQLTPLRGSKLWQLAALNLWLSEQGL
HCCCCCCCCCCCHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]