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The map label for this gene is mcrB [H]

Identifier: 66046953

GI number: 66046953

Start: 4433777

End: 4435330

Strand: Reverse

Name: mcrB [H]

Synonym: Psyr_3725

Alternate gene names: 66046953

Gene position: 4435330-4433777 (Counterclockwise)

Preceding gene: 66046955

Following gene: 66046952

Centisome position: 72.79

GC content: 50.9

Gene sequence:

>1554_bases
ATGCCTTCGCTCAATCAGATTTTCTTCGGTCCGCCCGGTACGGGGAAAACCTACGCCACAGTCGAAGCTACGTTGCAGAT
CCTTGATCAGCCTTTTCTTGCTAAAAACCTTGATAATCGGTCAGCGCTGAAAGCTCGATTTGACGAGCTGCTTGCAGCAG
GCGACGTGCGCTTTGTGACCTTTCATCAGAGCTTCAGCTACGAAGACTTTGTTGAAGGGCTTCGCGCTACGACTGATGAG
CAAGGCCAGATCCGTTACGAAGTGGTCTCAGGCGTTTTCAAAAGCCTCTGCGAGTCAATTGCGAGTGAACTGAGCGGAAA
GTACCGAGCATTCAAGGTTGGTGAGCGCTACGGCACAGGCTATAAGGTAATTCGAGCAAATGACGATATTCTTGAGTTAG
AAAAGCCAAAAGGTAAAAATTTTGGCCTTGCTATGAGTCTACTTAATGCTCTGGCTGATGACGTTTCTCAAGGTGTACTG
AGTGTTAATGATTTGAGCACAGGTAATTGGGAGGAAAAACTACCGAACTCTACTTATGATCCTTACTTGGTCAAAGGTTA
TCGCAATATTGTTCCCGTATTGATTGAACACATGCTCAGCAAGCGAAATGAAGATTTCCGGACGGCAGAGGTCGCTCAGA
GTGAACGGCCAAAAGTGCTGATCATTGATGAGATCAACCGAGGCAACGTCTCGCGCATCTTCGGCGAGCTGATTACCCTC
ATCGAACCTTCCAAGCGTGCGGGCGCATCAGAGGCACTCGAGGTTACGCTGCCCTACTCGAAAGAGCGCTTCTGCATCCC
CAGCAACATCCACTTGATCGGCACCATGAACACATCTGACCGATCATTGGCCGCACTTGATATTGCCCTGCGTCGTCGCT
TCACGTTCATTGAGGTGCCGCCTAACCCGGAGCTGCTTGAAGACATAGAGGTTGACGGCATCGCTATCGACGAATTGCTC
AGTGTGATGAACCAGCGTATAGCCGTCCTGCTTGATCAAGATCATTGCCTGGGTCACGCGTACTTTATGCCTCTCGAGAG
CGATCCGACCTTGGAGCGGCTGGCAGGTATTTTTCAAGAGCAAATCCTGCCGCTACTTCAGGAGTATTTCTTCGAAGATT
GGCAGCGTATCCAGTGGGTCCTCAACGACCAACGCAAAGCTCCAGAAAATAGGTTCTTGATCCAGCCGAGCCAAGACTTC
AGCGTGCTGTTTGGAGATGCTGTCACAGTGGGGCAGAACAACGAACGTTGGGAATTGAATCTGCCTGCATTCGACAATAT
CGAGTCATATCTGGGCGTGATCGATCACAACCTGAGCGTCAGCGCGCCGCTGGAAGCGAAGAGCGTCAGAACGGAAGGCG
TAGACATACGACAAGCCGCCAATGGCAGTATTGAGGTCTATCGTGGAGGCCAGCAGGTCAAACCTGCCAAGCCACATTTG
CGAGAGATTGCCAGCAAACATGGCCTACCTATTACATCAGCGTCGGGCATCAAGCTGAACACGCGGAGCCTCGGGCGTAA
GATCATCAAGTTCCTTAGCGAACAGCAAAGGTGA

Upstream 100 bases:

>100_bases
TCAGGGCTGCTACATAAACACGACACCACCGACGCTTCGCTTTACCCATGCTGGCAATCTAGATACTGTTGTCGGACTTT
TCCTACAGGGACGTTCGGCA

Downstream 100 bases:

>100_bases
AAACCACCGTAACGGTCCGAGAATATGCTCGGCTGACAACCAGCAATGTTCTTAGCCCGACGCTCGACGTAGCCCAGGTA
TCGCCCTCGGCTTTCGACTG

Product: ATPas

Products: NA

Alternate protein names: EcoKMcrBC [H]

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MPSLNQIFFGPPGTGKTYATVEATLQILDQPFLAKNLDNRSALKARFDELLAAGDVRFVTFHQSFSYEDFVEGLRATTDE
QGQIRYEVVSGVFKSLCESIASELSGKYRAFKVGERYGTGYKVIRANDDILELEKPKGKNFGLAMSLLNALADDVSQGVL
SVNDLSTGNWEEKLPNSTYDPYLVKGYRNIVPVLIEHMLSKRNEDFRTAEVAQSERPKVLIIDEINRGNVSRIFGELITL
IEPSKRAGASEALEVTLPYSKERFCIPSNIHLIGTMNTSDRSLAALDIALRRRFTFIEVPPNPELLEDIEVDGIAIDELL
SVMNQRIAVLLDQDHCLGHAYFMPLESDPTLERLAGIFQEQILPLLQEYFFEDWQRIQWVLNDQRKAPENRFLIQPSQDF
SVLFGDAVTVGQNNERWELNLPAFDNIESYLGVIDHNLSVSAPLEAKSVRTEGVDIRQAANGSIEVYRGGQQVKPAKPHL
REIASKHGLPITSASGIKLNTRSLGRKIIKFLSEQQR

Sequences:

>Translated_517_residues
MPSLNQIFFGPPGTGKTYATVEATLQILDQPFLAKNLDNRSALKARFDELLAAGDVRFVTFHQSFSYEDFVEGLRATTDE
QGQIRYEVVSGVFKSLCESIASELSGKYRAFKVGERYGTGYKVIRANDDILELEKPKGKNFGLAMSLLNALADDVSQGVL
SVNDLSTGNWEEKLPNSTYDPYLVKGYRNIVPVLIEHMLSKRNEDFRTAEVAQSERPKVLIIDEINRGNVSRIFGELITL
IEPSKRAGASEALEVTLPYSKERFCIPSNIHLIGTMNTSDRSLAALDIALRRRFTFIEVPPNPELLEDIEVDGIAIDELL
SVMNQRIAVLLDQDHCLGHAYFMPLESDPTLERLAGIFQEQILPLLQEYFFEDWQRIQWVLNDQRKAPENRFLIQPSQDF
SVLFGDAVTVGQNNERWELNLPAFDNIESYLGVIDHNLSVSAPLEAKSVRTEGVDIRQAANGSIEVYRGGQQVKPAKPHL
REIASKHGLPITSASGIKLNTRSLGRKIIKFLSEQQR
>Mature_516_residues
PSLNQIFFGPPGTGKTYATVEATLQILDQPFLAKNLDNRSALKARFDELLAAGDVRFVTFHQSFSYEDFVEGLRATTDEQ
GQIRYEVVSGVFKSLCESIASELSGKYRAFKVGERYGTGYKVIRANDDILELEKPKGKNFGLAMSLLNALADDVSQGVLS
VNDLSTGNWEEKLPNSTYDPYLVKGYRNIVPVLIEHMLSKRNEDFRTAEVAQSERPKVLIIDEINRGNVSRIFGELITLI
EPSKRAGASEALEVTLPYSKERFCIPSNIHLIGTMNTSDRSLAALDIALRRRFTFIEVPPNPELLEDIEVDGIAIDELLS
VMNQRIAVLLDQDHCLGHAYFMPLESDPTLERLAGIFQEQILPLLQEYFFEDWQRIQWVLNDQRKAPENRFLIQPSQDFS
VLFGDAVTVGQNNERWELNLPAFDNIESYLGVIDHNLSVSAPLEAKSVRTEGVDIRQAANGSIEVYRGGQQVKPAKPHLR
EIASKHGLPITSASGIKLNTRSLGRKIIKFLSEQQR

Specific function: Recognizes N4- and C5-methylcytosine (and 5-hydroxy- methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Is

COG id: COG1401

COG function: function code V; GTPase subunit of restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082429, Length=187, Percent_Identity=39.572192513369, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011704
- InterPro:   IPR021961
- InterPro:   IPR004491 [H]

Pfam domain/function: PF07728 AAA_5; PF12102 DUF3578 [H]

EC number: 3.1.21.- [C]

Molecular weight: Translated: 58077; Mature: 57945

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSLNQIFFGPPGTGKTYATVEATLQILDQPFLAKNLDNRSALKARFDELLAAGDVRFVT
CCCCCCEEECCCCCCCEEEHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHCCCEEEEE
FHQSFSYEDFVEGLRATTDEQGQIRYEVVSGVFKSLCESIASELSGKYRAFKVGERYGTG
EECCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCC
YKVIRANDDILELEKPKGKNFGLAMSLLNALADDVSQGVLSVNDLSTGNWEEKLPNSTYD
EEEEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCCHHHCCCCCCC
PYLVKGYRNIVPVLIEHMLSKRNEDFRTAEVAQSERPKVLIIDEINRGNVSRIFGELITL
CHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHH
IEPSKRAGASEALEVTLPYSKERFCIPSNIHLIGTMNTSDRSLAALDIALRRRFTFIEVP
CCCCHHCCCCCEEEEEECCCCCCEECCCCEEEEEECCCCCCHHHHHHHHHHCCEEEEECC
PNPELLEDIEVDGIAIDELLSVMNQRIAVLLDQDHCLGHAYFMPLESDPTLERLAGIFQE
CCCHHHHHCCCCCEEHHHHHHHHHCCEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHH
QILPLLQEYFFEDWQRIQWVLNDQRKAPENRFLIQPSQDFSVLFGDAVTVGQNNERWELN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCEEEECCEEEECCCCCEEEEE
LPAFDNIESYLGVIDHNLSVSAPLEAKSVRTEGVDIRQAANGSIEVYRGGQQVKPAKPHL
CCCHHCHHHHHHHHCCCCCCCCCCCHHHHCCCCCCHHHCCCCCEEEEECCCCCCCCCHHH
REIASKHGLPITSASGIKLNTRSLGRKIIKFLSEQQR
HHHHHHCCCCEECCCCCEECHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PSLNQIFFGPPGTGKTYATVEATLQILDQPFLAKNLDNRSALKARFDELLAAGDVRFVT
CCCCCEEECCCCCCCEEEHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHCCCEEEEE
FHQSFSYEDFVEGLRATTDEQGQIRYEVVSGVFKSLCESIASELSGKYRAFKVGERYGTG
EECCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCC
YKVIRANDDILELEKPKGKNFGLAMSLLNALADDVSQGVLSVNDLSTGNWEEKLPNSTYD
EEEEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCCHHHCCCCCCC
PYLVKGYRNIVPVLIEHMLSKRNEDFRTAEVAQSERPKVLIIDEINRGNVSRIFGELITL
CHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHH
IEPSKRAGASEALEVTLPYSKERFCIPSNIHLIGTMNTSDRSLAALDIALRRRFTFIEVP
CCCCHHCCCCCEEEEEECCCCCCEECCCCEEEEEECCCCCCHHHHHHHHHHCCEEEEECC
PNPELLEDIEVDGIAIDELLSVMNQRIAVLLDQDHCLGHAYFMPLESDPTLERLAGIFQE
CCCHHHHHCCCCCEEHHHHHHHHHCCEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHH
QILPLLQEYFFEDWQRIQWVLNDQRKAPENRFLIQPSQDFSVLFGDAVTVGQNNERWELN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCEEEECCEEEECCCCCEEEEE
LPAFDNIESYLGVIDHNLSVSAPLEAKSVRTEGVDIRQAANGSIEVYRGGQQVKPAKPHL
CCCHHCHHHHHHHHCCCCCCCCCCCHHHHCCCCCCHHHCCCCCEEEEECCCCCCCCCHHH
REIASKHGLPITSASGIKLNTRSLGRKIIKFLSEQQR
HHHHHHCCCCEECCCCCEECHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Endodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2203735; 2649480; 7610040; 9278503; 1312983; 7781618 [H]