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The map label for this gene is gacS [H]

Identifier: 66046841

GI number: 66046841

Start: 4295384

End: 4297774

Strand: Direct

Name: gacS [H]

Synonym: Psyr_3612

Alternate gene names: 66046841

Gene position: 4295384-4297774 (Clockwise)

Preceding gene: 66046838

Following gene: 66046844

Centisome position: 70.49

GC content: 61.9

Gene sequence:

>2391_bases
ATGCTTGGCCGGCACCGGGCACCAGAAGTGGCAGTACTCATGGATATATCGCTCAAAAACAGGCTGTCGTTCAAGCAGGC
GCGCCTGGCTGTATCGATCGGCTTCGTGCTGGGGACCCTGCTGAGCCTGTTCCAGATTGCGATCGATTATGCCAGCGAAG
ACGCTTCCATCAATCGTGAAATCAAGTCACTGCTGGAAATCATCCAGAACCCGGCGTCGCGCATCGCCTATAACATCGAC
TCGGAGCTGGCTCAGGAACTCACGCTCGGCCTGCTGCGCTCGCCTGCCGTGGTCAGCGCCAGGCTTACCGATAACAACGA
CGCCGTGCTCTCCAGCGTCGAGCGCCCCATGGCCACCGGGCGTTATCGGGTGTTCAGCGACTGGCTGTTCGGCGAAAGCC
GGCAGTTTCAGGAAAAGCTGCACCTCAATCACCTGCCCAATGAAACCATCGGCACACTGTACCTGACCGTGGACACCTTC
GCGTTCGGCAGCCACTTTCTGCAGCGCGCCGAAATCACCCTGCTCAACGGCTTTGTGCGCAGCCTGGCGCTGGCCGGGAT
TCTGACCATTCTGTTTTACGCGACCCTGACCAAGCCGCTGGTGAGGGTAATCCGCGAACTGAGCAGTCGCGATCCGCGCA
ACCCTGACCAGGGCAAAATAACCTGCCCGCCCTACCATGCCCAGGATGAAATCGGCGTGCTGGTCGAGACGTTCAACCGT
CAGTTTGAAACCATGAGCAGCGAGTTTTCCAGGCGACGCGCGGCCGAGGACCGGCTGACCGACCACCTCAATGAACTGGA
AAACATCGTCTCGGCGCGCACCAACGAGCTCAAGGCCAGCAACCTGCGCCTGCGCGAATCCAACGAGGAACTGCAGATAG
CACGCAGCACGGCGCTGGACATGGCCCATGCGCGCTCGGCATTTCTGGCGCACATGAGCCATGAGATTCGTACACCGCTC
AACGGTCTGCTGGGCATGCTGGCGCTGTCGCTGGACAGTCCGCTGGGTGCCGAACAACGCCAGCAGCTAATGATCGCCCA
CGACTCAGGCAAGGTGCTGGTCGAGCTGCTCAACGATATTCTCGACATGTCCAAGTTCGATGCCGGTCACCTGGAGATCG
AGCGCATTCCTTTCGACCTTGGGATGTTGATCGAGGACACCGCCAATCTGCTGTCGCAGAACGCAGCGCCCAGCGTGGAG
CTGACCTGCCTGATCGCGCCGGACTTCCCGGCCATGGTCACCGGCGATCCGACCAGGGTGCGTCAGATCGTCAGCAACCT
GCTGTCCAACGCCCTCAAGTTCACCCGTTTCGGGCGCGTCGATGTGCGTCTGACTCACCATCGGGACGCCAGCTCCGGTG
AACACCGGGTGCGCATCGAAGTCCGCGACACCGGCATCGGCATTGCGCAGGACGCCCAGACCCGAATCTTCCAGCCGTTC
ACCCAGGCTGAGACGGCCATCACCCGCCAGTACGGCGGTACGGGACTGGGCCTGGCGCTGACCAACAACCTTTGCGAGGC
CATGAACGGCACGTTGAGCATCCAGTCGCAGTCCGGTTTCGGCAGCCAGTTCTGCGCCGAATTGCCGTTGCCGGTCCATG
TGCCCGCGGTCACTCAGACGCCGCTAAAGGGCCGGGTGACCGTGATCAGTTCGGCAGGCAGCGGACTGGTGGAGCTGCTG
GGCAGTCTGCTGCCGTTCTGGGGGGTCGACCTCAAGCGACGCAGCATCGACGATCGCGCACAAGCCGTGGACCTCGACGG
CAGTTCCGATCTGGTCATCACCGACTGTCCCGAATGCCTGTTTACGATTCGCCCCACCACACCCGTGCCGATCCTGCTGG
TGACCGCCTATGGCAACTTCATGCCCGGCGAACAGGTTGCCGCCCTGGCCCCGCTGCAACAGCAGGCCCGGCCACTGGCG
CGCAACGCGCTTTACCACACGCTGCGGCGCATACTGGGTCAGGAAGAAACGGGGCTCAAGGACACACAGGCGCTCGAACA
TACGGCGCCACGTCTGGCGCGGGTGTTATTGGTCGAGGACAATCCGGTCAATCAACTGGTCGCCAAGGGCATCCTCGGCA
AACTGGGTTGCGAAGTGATCATCAGCAGCCATGGCGGCGAAGCCCTGGAACAGCTGGAGCAAGGCCATTTCGATCTGGTA
TTGATGGACTGCAACATGCCGGTCATGGACGGCTATGAAGCCAGCCGCCAGATCCGCAGCAGCGGCCGCTGGCCGAACCT
GCCGATCGTTGCCCTTACCGCCAACGCCATGCCCGACGAACGCGAGCGCTGCAAGGCGGCGGGCATGAATGACTATCTGG
CCAAGCCGTTCCGGCGTGCCGAACTGGTCAGCATTCTCGATCAGTGGATACCCATTACCGGCATCGACTGA

Upstream 100 bases:

>100_bases
TCGGGCCAGCACGGCGTCGGCGCGCTGCAGAAAAGCATCCAACCGAGAATCCACGGGATCTCCTTATATTTAACTGATTA
GTCAGCAGTGCCGATCGGCT

Downstream 100 bases:

>100_bases
AGACTCAGCGCAGCAGCGCCTCGATCTCGCCCGTCAGTTCCTCAGGCTTGGTCAATGGCGCAAAACGCTTGACCAGCCGA
CCGTCCTGGCCAATCAGGAA

Product: response regulator receiver:ATP-binding region, ATPase-like:histidine kinase A, N-terminal

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 796; Mature: 796

Protein sequence:

>796_residues
MLGRHRAPEVAVLMDISLKNRLSFKQARLAVSIGFVLGTLLSLFQIAIDYASEDASINREIKSLLEIIQNPASRIAYNID
SELAQELTLGLLRSPAVVSARLTDNNDAVLSSVERPMATGRYRVFSDWLFGESRQFQEKLHLNHLPNETIGTLYLTVDTF
AFGSHFLQRAEITLLNGFVRSLALAGILTILFYATLTKPLVRVIRELSSRDPRNPDQGKITCPPYHAQDEIGVLVETFNR
QFETMSSEFSRRRAAEDRLTDHLNELENIVSARTNELKASNLRLRESNEELQIARSTALDMAHARSAFLAHMSHEIRTPL
NGLLGMLALSLDSPLGAEQRQQLMIAHDSGKVLVELLNDILDMSKFDAGHLEIERIPFDLGMLIEDTANLLSQNAAPSVE
LTCLIAPDFPAMVTGDPTRVRQIVSNLLSNALKFTRFGRVDVRLTHHRDASSGEHRVRIEVRDTGIGIAQDAQTRIFQPF
TQAETAITRQYGGTGLGLALTNNLCEAMNGTLSIQSQSGFGSQFCAELPLPVHVPAVTQTPLKGRVTVISSAGSGLVELL
GSLLPFWGVDLKRRSIDDRAQAVDLDGSSDLVITDCPECLFTIRPTTPVPILLVTAYGNFMPGEQVAALAPLQQQARPLA
RNALYHTLRRILGQEETGLKDTQALEHTAPRLARVLLVEDNPVNQLVAKGILGKLGCEVIISSHGGEALEQLEQGHFDLV
LMDCNMPVMDGYEASRQIRSSGRWPNLPIVALTANAMPDERERCKAAGMNDYLAKPFRRAELVSILDQWIPITGID

Sequences:

>Translated_796_residues
MLGRHRAPEVAVLMDISLKNRLSFKQARLAVSIGFVLGTLLSLFQIAIDYASEDASINREIKSLLEIIQNPASRIAYNID
SELAQELTLGLLRSPAVVSARLTDNNDAVLSSVERPMATGRYRVFSDWLFGESRQFQEKLHLNHLPNETIGTLYLTVDTF
AFGSHFLQRAEITLLNGFVRSLALAGILTILFYATLTKPLVRVIRELSSRDPRNPDQGKITCPPYHAQDEIGVLVETFNR
QFETMSSEFSRRRAAEDRLTDHLNELENIVSARTNELKASNLRLRESNEELQIARSTALDMAHARSAFLAHMSHEIRTPL
NGLLGMLALSLDSPLGAEQRQQLMIAHDSGKVLVELLNDILDMSKFDAGHLEIERIPFDLGMLIEDTANLLSQNAAPSVE
LTCLIAPDFPAMVTGDPTRVRQIVSNLLSNALKFTRFGRVDVRLTHHRDASSGEHRVRIEVRDTGIGIAQDAQTRIFQPF
TQAETAITRQYGGTGLGLALTNNLCEAMNGTLSIQSQSGFGSQFCAELPLPVHVPAVTQTPLKGRVTVISSAGSGLVELL
GSLLPFWGVDLKRRSIDDRAQAVDLDGSSDLVITDCPECLFTIRPTTPVPILLVTAYGNFMPGEQVAALAPLQQQARPLA
RNALYHTLRRILGQEETGLKDTQALEHTAPRLARVLLVEDNPVNQLVAKGILGKLGCEVIISSHGGEALEQLEQGHFDLV
LMDCNMPVMDGYEASRQIRSSGRWPNLPIVALTANAMPDERERCKAAGMNDYLAKPFRRAELVSILDQWIPITGID
>Mature_796_residues
MLGRHRAPEVAVLMDISLKNRLSFKQARLAVSIGFVLGTLLSLFQIAIDYASEDASINREIKSLLEIIQNPASRIAYNID
SELAQELTLGLLRSPAVVSARLTDNNDAVLSSVERPMATGRYRVFSDWLFGESRQFQEKLHLNHLPNETIGTLYLTVDTF
AFGSHFLQRAEITLLNGFVRSLALAGILTILFYATLTKPLVRVIRELSSRDPRNPDQGKITCPPYHAQDEIGVLVETFNR
QFETMSSEFSRRRAAEDRLTDHLNELENIVSARTNELKASNLRLRESNEELQIARSTALDMAHARSAFLAHMSHEIRTPL
NGLLGMLALSLDSPLGAEQRQQLMIAHDSGKVLVELLNDILDMSKFDAGHLEIERIPFDLGMLIEDTANLLSQNAAPSVE
LTCLIAPDFPAMVTGDPTRVRQIVSNLLSNALKFTRFGRVDVRLTHHRDASSGEHRVRIEVRDTGIGIAQDAQTRIFQPF
TQAETAITRQYGGTGLGLALTNNLCEAMNGTLSIQSQSGFGSQFCAELPLPVHVPAVTQTPLKGRVTVISSAGSGLVELL
GSLLPFWGVDLKRRSIDDRAQAVDLDGSSDLVITDCPECLFTIRPTTPVPILLVTAYGNFMPGEQVAALAPLQQQARPLA
RNALYHTLRRILGQEETGLKDTQALEHTAPRLARVLLVEDNPVNQLVAKGILGKLGCEVIISSHGGEALEQLEQGHFDLV
LMDCNMPVMDGYEASRQIRSSGRWPNLPIVALTANAMPDERERCKAAGMNDYLAKPFRRAELVSILDQWIPITGID

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI145693157, Length=509, Percent_Identity=34.1846758349705, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI1789149, Length=278, Percent_Identity=38.8489208633094, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI87081816, Length=371, Percent_Identity=38.2749326145553, Blast_Score=184, Evalue=2e-47,
Organism=Escherichia coli, GI1788713, Length=248, Percent_Identity=35.0806451612903, Blast_Score=145, Evalue=1e-35,
Organism=Escherichia coli, GI48994928, Length=238, Percent_Identity=36.9747899159664, Blast_Score=143, Evalue=6e-35,
Organism=Escherichia coli, GI1790436, Length=225, Percent_Identity=31.5555555555556, Blast_Score=93, Evalue=6e-20,
Organism=Escherichia coli, GI1786600, Length=233, Percent_Identity=31.7596566523605, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1788393, Length=229, Percent_Identity=32.7510917030568, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87082128, Length=231, Percent_Identity=29.4372294372294, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1786783, Length=233, Percent_Identity=26.6094420600858, Blast_Score=76, Evalue=7e-15,
Organism=Escherichia coli, GI1787894, Length=228, Percent_Identity=28.0701754385965, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1790346, Length=237, Percent_Identity=27.0042194092827, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1788549, Length=278, Percent_Identity=24.4604316546763, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1786912, Length=258, Percent_Identity=25.968992248062, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1789808, Length=232, Percent_Identity=25.4310344827586, Blast_Score=67, Evalue=5e-12,
Organism=Escherichia coli, GI1790300, Length=259, Percent_Identity=27.027027027027, Blast_Score=64, Evalue=6e-11,
Organism=Escherichia coli, GI1788191, Length=121, Percent_Identity=31.404958677686, Blast_Score=63, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6322000, Length=117, Percent_Identity=42.7350427350427, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6322044, Length=74, Percent_Identity=47.2972972972973, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323034, Length=154, Percent_Identity=29.8701298701299, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 87811; Mature: 87811

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGRHRAPEVAVLMDISLKNRLSFKQARLAVSIGFVLGTLLSLFQIAIDYASEDASINRE
CCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
IKSLLEIIQNPASRIAYNIDSELAQELTLGLLRSPAVVSARLTDNNDAVLSSVERPMATG
HHHHHHHHHCHHHHHEECCHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHCC
RYRVFSDWLFGESRQFQEKLHLNHLPNETIGTLYLTVDTFAFGSHFLQRAEITLLNGFVR
CHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHH
SLALAGILTILFYATLTKPLVRVIRELSSRDPRNPDQGKITCPPYHAQDEIGVLVETFNR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHH
QFETMSSEFSRRRAAEDRLTDHLNELENIVSARTNELKASNLRLRESNEELQIARSTALD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHH
MAHARSAFLAHMSHEIRTPLNGLLGMLALSLDSPLGAEQRQQLMIAHDSGKVLVELLNDI
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHCEEEEEECCCHHHHHHHHHH
LDMSKFDAGHLEIERIPFDLGMLIEDTANLLSQNAAPSVELTCLIAPDFPAMVTGDPTRV
HHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEECCCHHHH
RQIVSNLLSNALKFTRFGRVDVRLTHHRDASSGEHRVRIEVRDTGIGIAQDAQTRIFQPF
HHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEEEEECCCCCCCCCHHHHHCCH
TQAETAITRQYGGTGLGLALTNNLCEAMNGTLSIQSQSGFGSQFCAELPLPVHVPAVTQT
HHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCHHHHHCCCCCEECCCCCCC
PLKGRVTVISSAGSGLVELLGSLLPFWGVDLKRRSIDDRAQAVDLDGSSDLVITDCPECL
CCCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHCCCCCHHHEEECCCCCCEEEECCCCCE
FTIRPTTPVPILLVTAYGNFMPGEQVAALAPLQQQARPLARNALYHTLRRILGQEETGLK
EEECCCCCCCEEEEEECCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DTQALEHTAPRLARVLLVEDNPVNQLVAKGILGKLGCEVIISSHGGEALEQLEQGHFDLV
HHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHCCCCEEE
LMDCNMPVMDGYEASRQIRSSGRWPNLPIVALTANAMPDERERCKAAGMNDYLAKPFRRA
EEECCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHCCCCHHHHCHHHHH
ELVSILDQWIPITGID
HHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLGRHRAPEVAVLMDISLKNRLSFKQARLAVSIGFVLGTLLSLFQIAIDYASEDASINRE
CCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
IKSLLEIIQNPASRIAYNIDSELAQELTLGLLRSPAVVSARLTDNNDAVLSSVERPMATG
HHHHHHHHHCHHHHHEECCHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHCC
RYRVFSDWLFGESRQFQEKLHLNHLPNETIGTLYLTVDTFAFGSHFLQRAEITLLNGFVR
CHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHH
SLALAGILTILFYATLTKPLVRVIRELSSRDPRNPDQGKITCPPYHAQDEIGVLVETFNR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHH
QFETMSSEFSRRRAAEDRLTDHLNELENIVSARTNELKASNLRLRESNEELQIARSTALD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHH
MAHARSAFLAHMSHEIRTPLNGLLGMLALSLDSPLGAEQRQQLMIAHDSGKVLVELLNDI
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHCEEEEEECCCHHHHHHHHHH
LDMSKFDAGHLEIERIPFDLGMLIEDTANLLSQNAAPSVELTCLIAPDFPAMVTGDPTRV
HHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEECCCHHHH
RQIVSNLLSNALKFTRFGRVDVRLTHHRDASSGEHRVRIEVRDTGIGIAQDAQTRIFQPF
HHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEEEEECCCCCCCCCHHHHHCCH
TQAETAITRQYGGTGLGLALTNNLCEAMNGTLSIQSQSGFGSQFCAELPLPVHVPAVTQT
HHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCHHHHHCCCCCEECCCCCCC
PLKGRVTVISSAGSGLVELLGSLLPFWGVDLKRRSIDDRAQAVDLDGSSDLVITDCPECL
CCCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHCCCCCHHHEEECCCCCCEEEECCCCCE
FTIRPTTPVPILLVTAYGNFMPGEQVAALAPLQQQARPLARNALYHTLRRILGQEETGLK
EEECCCCCCCEEEEEECCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DTQALEHTAPRLARVLLVEDNPVNQLVAKGILGKLGCEVIISSHGGEALEQLEQGHFDLV
HHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHCCCCEEE
LMDCNMPVMDGYEASRQIRSSGRWPNLPIVALTANAMPDERERCKAAGMNDYLAKPFRRA
EEECCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHCCCCHHHHCHHHHH
ELVSILDQWIPITGID
HHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]