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The map label for this gene is fliF [H]

Identifier: 66046686

GI number: 66046686

Start: 4120078

End: 4121862

Strand: Reverse

Name: fliF [H]

Synonym: Psyr_3457

Alternate gene names: 66046686

Gene position: 4121862-4120078 (Counterclockwise)

Preceding gene: 66046687

Following gene: 66046685

Centisome position: 67.64

GC content: 60.45

Gene sequence:

>1785_bases
ATGGCCGAAGCAGCTGCCGATACCGTTCCTGCAAGAACGGGCGATTCCGAAAGAAAACCGTTGTTCGGTATGTCTTTTCT
GGAAAATCTTTCCGAAATGACCATGCTGCGTCAGATCGGCCTCATGGTCGGTCTTGCCGCGAGCGTGGCCATCGGCTTCG
CCGTGGTGCTCTGGTCCCAGCAGCCTGACTATCGTCCGCTTTATGGCAGTCTGGCGGGCATGGACAGCAAGCAGATCATG
GACACCCTGACTGCGGCGAACATCAACTACACCGTTGAGCCGAACTCCGGTGCGCTGCTGGTCAAGTCCGATGATGTGCA
GCGTGCGCGTATCCAACTGGCCCAGGCGGGTGTGGTACAGAACGACGCCAACATCGGTTTCGAAATCCTTGACAAGGATC
AGGGCCTTGGCACCAGTCAGTTCATGGAAGCCACACGCTATCGTCGTGGCCTGGAAGGTGAACTGGCACGTACCATTTCT
GCATTGAACAACGTCAAGGGTGCCCGTGTGCACCTGGCCATTCCGAAGAGCTCGGTGTTCGTCCGTGATGACCGCAAGCC
AAGTGCATCGGTACTGGTCGAGCTGTACGCCGGTCGTTCACTGGAGCCGAGTCAGGTCATGGCCATCATCAATCTGGTGG
CCACCAGTGTTCCCGAGCTGAGCAAGTCGCAGATTACCGTCGTCGACCAGAAGGGCGCTCTGCTTTCCGATCAGGCGGAA
AACTCCGAACTGACCATGGCCGGCAAGCAATTCGACTACAGCCGTCGCATGGAAGGCATGCTGACCCAGCGCGTGCAGAA
CATTCTGCAGCCGATTCTGGGCAATGATCGCTACAAGGCCGAGGTCTCGGCAGTGGTTGATTTCAGCGCTGTCGAATCGA
CCGCCGAAAGCTTCAACCCCGACCAGCCTGCCTTGCGCAGCGAGCAGTCGGTCAATGAACAACGTTCCAGCAGCTCAAGC
TCGGGCGGCGTTCCGGGTGCGCTGAGCAACCAGCCGCCCGGCCCGGCGACGGCTCCGCAGACTGCAGGCGGTGGTGCCGC
CGGTGCTGCCGGCCCCATCGCGCCAGGCCAGCCACTGCTGGATGCCAATGGTCAGCAGATCATGGACCCGGCCACCGGTC
AGCCCGCGTTGGCGCCATACCCTGCCGACAAGCGCGTGCAGTCGACCAAGAACTTCGAGCTGGACCGTTCGATCAGTCAC
ACCAAGCAGCAGCAGGGCCGTCTGACCCGCCTGTCGGTCGCGGTGGTGGTCGACGACATGGTCAAGACCAACGCGGCCAA
TGGCGAAGTGACGCGTGCTCCGTGGAGCGCGGCGGATCTGGCTCGTTTCACGCGTCTGGTACAGGATGCCGTGGGTTTCG
ACGCCAGCCGCGGTGACAGCGTCAGCGTGATCAACGTGCCATTCTCCAGCGAACGCGCTGAAGTGCTGCCTGAGGCGTCG
TTCTATTCGCAACCCTGGTTCTGGGACATCGTCAAGCAGGCGGTTGGCGTGATTTTCATCCTGATCCTGGTGTTTGGTGT
ACTGCGCCCGGTGTTGAACAACATCACCAACGGCAAGCGCAAGGAACTGGCAGGTTTCGGTGGCGACGCCGAACTGGGTG
GCATGGGCGGTCTGGACGGCGAATTGTCCAACGATCGCGTAAGCCTGGGCGGCCCGCAGAGTATTCTGCTGCCGAGCCCG
ACCGAAGGCTATGACGCACAGCTGAACGCAATCAAGAGTTTGGTGGCTGAGGATCCTGGCCGTGTGGCTCAGGTTGTGAA
AGAGTGGATCAACACTGATGAATGA

Upstream 100 bases:

>100_bases
AGCCAGCGTGTCTTTTCAGGCGTTGACCCAGGTGCGTAACAAGCTGGTTCAAGCTTACCAAGACATCATGCAGATGCCGG
TTTAAGGGACGTACTGAGTC

Downstream 100 bases:

>100_bases
ACGAGCCATGGTAGCCAAACTCTCCAAAGTTGAAAAAGCGGCTGTACTGTTGCTCTCGCTGGGCGAAACCGATGCGGCTC
AGGTGCTCCGGCACATGGGC

Product: flagellar MS-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 594; Mature: 593

Protein sequence:

>594_residues
MAEAAADTVPARTGDSERKPLFGMSFLENLSEMTMLRQIGLMVGLAASVAIGFAVVLWSQQPDYRPLYGSLAGMDSKQIM
DTLTAANINYTVEPNSGALLVKSDDVQRARIQLAQAGVVQNDANIGFEILDKDQGLGTSQFMEATRYRRGLEGELARTIS
ALNNVKGARVHLAIPKSSVFVRDDRKPSASVLVELYAGRSLEPSQVMAIINLVATSVPELSKSQITVVDQKGALLSDQAE
NSELTMAGKQFDYSRRMEGMLTQRVQNILQPILGNDRYKAEVSAVVDFSAVESTAESFNPDQPALRSEQSVNEQRSSSSS
SGGVPGALSNQPPGPATAPQTAGGGAAGAAGPIAPGQPLLDANGQQIMDPATGQPALAPYPADKRVQSTKNFELDRSISH
TKQQQGRLTRLSVAVVVDDMVKTNAANGEVTRAPWSAADLARFTRLVQDAVGFDASRGDSVSVINVPFSSERAEVLPEAS
FYSQPWFWDIVKQAVGVIFILILVFGVLRPVLNNITNGKRKELAGFGGDAELGGMGGLDGELSNDRVSLGGPQSILLPSP
TEGYDAQLNAIKSLVAEDPGRVAQVVKEWINTDE

Sequences:

>Translated_594_residues
MAEAAADTVPARTGDSERKPLFGMSFLENLSEMTMLRQIGLMVGLAASVAIGFAVVLWSQQPDYRPLYGSLAGMDSKQIM
DTLTAANINYTVEPNSGALLVKSDDVQRARIQLAQAGVVQNDANIGFEILDKDQGLGTSQFMEATRYRRGLEGELARTIS
ALNNVKGARVHLAIPKSSVFVRDDRKPSASVLVELYAGRSLEPSQVMAIINLVATSVPELSKSQITVVDQKGALLSDQAE
NSELTMAGKQFDYSRRMEGMLTQRVQNILQPILGNDRYKAEVSAVVDFSAVESTAESFNPDQPALRSEQSVNEQRSSSSS
SGGVPGALSNQPPGPATAPQTAGGGAAGAAGPIAPGQPLLDANGQQIMDPATGQPALAPYPADKRVQSTKNFELDRSISH
TKQQQGRLTRLSVAVVVDDMVKTNAANGEVTRAPWSAADLARFTRLVQDAVGFDASRGDSVSVINVPFSSERAEVLPEAS
FYSQPWFWDIVKQAVGVIFILILVFGVLRPVLNNITNGKRKELAGFGGDAELGGMGGLDGELSNDRVSLGGPQSILLPSP
TEGYDAQLNAIKSLVAEDPGRVAQVVKEWINTDE
>Mature_593_residues
AEAAADTVPARTGDSERKPLFGMSFLENLSEMTMLRQIGLMVGLAASVAIGFAVVLWSQQPDYRPLYGSLAGMDSKQIMD
TLTAANINYTVEPNSGALLVKSDDVQRARIQLAQAGVVQNDANIGFEILDKDQGLGTSQFMEATRYRRGLEGELARTISA
LNNVKGARVHLAIPKSSVFVRDDRKPSASVLVELYAGRSLEPSQVMAIINLVATSVPELSKSQITVVDQKGALLSDQAEN
SELTMAGKQFDYSRRMEGMLTQRVQNILQPILGNDRYKAEVSAVVDFSAVESTAESFNPDQPALRSEQSVNEQRSSSSSS
GGVPGALSNQPPGPATAPQTAGGGAAGAAGPIAPGQPLLDANGQQIMDPATGQPALAPYPADKRVQSTKNFELDRSISHT
KQQQGRLTRLSVAVVVDDMVKTNAANGEVTRAPWSAADLARFTRLVQDAVGFDASRGDSVSVINVPFSSERAEVLPEASF
YSQPWFWDIVKQAVGVIFILILVFGVLRPVLNNITNGKRKELAGFGGDAELGGMGGLDGELSNDRVSLGGPQSILLPSPT
EGYDAQLNAIKSLVAEDPGRVAQVVKEWINTDE

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=467, Percent_Identity=39.6145610278373, Blast_Score=288, Evalue=6e-79,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 63315; Mature: 63184

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEAAADTVPARTGDSERKPLFGMSFLENLSEMTMLRQIGLMVGLAASVAIGFAVVLWSQ
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECC
QPDYRPLYGSLAGMDSKQIMDTLTAANINYTVEPNSGALLVKSDDVQRARIQLAQAGVVQ
CCCCCCHHHHHHCCCHHHHHHHHHHCCCEEEEECCCCEEEEECCCHHHHHHHHHHHCCEE
NDANIGFEILDKDQGLGTSQFMEATRYRRGLEGELARTISALNNVKGARVHLAIPKSSVF
CCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEECCCCEE
VRDDRKPSASVLVELYAGRSLEPSQVMAIINLVATSVPELSKSQITVVDQKGALLSDQAE
EECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEECCCCC
NSELTMAGKQFDYSRRMEGMLTQRVQNILQPILGNDRYKAEVSAVVDFSAVESTAESFNP
CCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
DQPALRSEQSVNEQRSSSSSSGGVPGALSNQPPGPATAPQTAGGGAAGAAGPIAPGQPLL
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DANGQQIMDPATGQPALAPYPADKRVQSTKNFELDRSISHTKQQQGRLTRLSVAVVVDDM
CCCCCEECCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHCCCHHEEHHHHHHHHH
VKTNAANGEVTRAPWSAADLARFTRLVQDAVGFDASRGDSVSVINVPFSSERAEVLPEAS
HHCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHCCCCH
FYSQPWFWDIVKQAVGVIFILILVFGVLRPVLNNITNGKRKELAGFGGDAELGGMGGLDG
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCC
ELSNDRVSLGGPQSILLPSPTEGYDAQLNAIKSLVAEDPGRVAQVVKEWINTDE
CCCCCEEECCCCCEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AEAAADTVPARTGDSERKPLFGMSFLENLSEMTMLRQIGLMVGLAASVAIGFAVVLWSQ
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECC
QPDYRPLYGSLAGMDSKQIMDTLTAANINYTVEPNSGALLVKSDDVQRARIQLAQAGVVQ
CCCCCCHHHHHHCCCHHHHHHHHHHCCCEEEEECCCCEEEEECCCHHHHHHHHHHHCCEE
NDANIGFEILDKDQGLGTSQFMEATRYRRGLEGELARTISALNNVKGARVHLAIPKSSVF
CCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEECCCCEE
VRDDRKPSASVLVELYAGRSLEPSQVMAIINLVATSVPELSKSQITVVDQKGALLSDQAE
EECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEECCCCC
NSELTMAGKQFDYSRRMEGMLTQRVQNILQPILGNDRYKAEVSAVVDFSAVESTAESFNP
CCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
DQPALRSEQSVNEQRSSSSSSGGVPGALSNQPPGPATAPQTAGGGAAGAAGPIAPGQPLL
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DANGQQIMDPATGQPALAPYPADKRVQSTKNFELDRSISHTKQQQGRLTRLSVAVVVDDM
CCCCCEECCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHCCCHHEEHHHHHHHHH
VKTNAANGEVTRAPWSAADLARFTRLVQDAVGFDASRGDSVSVINVPFSSERAEVLPEAS
HHCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHCCCCH
FYSQPWFWDIVKQAVGVIFILILVFGVLRPVLNNITNGKRKELAGFGGDAELGGMGGLDG
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCC
ELSNDRVSLGGPQSILLPSPTEGYDAQLNAIKSLVAEDPGRVAQVVKEWINTDE
CCCCCEEECCCCCEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8675317; 10984043 [H]