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The map label for this gene is fliM [H]

Identifier: 66046676

GI number: 66046676

Start: 4109999

End: 4110967

Strand: Reverse

Name: fliM [H]

Synonym: Psyr_3447

Alternate gene names: 66046676

Gene position: 4110967-4109999 (Counterclockwise)

Preceding gene: 66046677

Following gene: 66046675

Centisome position: 67.46

GC content: 59.86

Gene sequence:

>969_bases
ATGGCCGTGCAAGACCTGCTCTCCCAGGATGAGATCGATGCGCTGCTGCATGGCGTCGACGATGGGATGGTCCAGACCGA
CAACAACAGCGAACCTGGCAGCGTCAAAAGTTACGACCTGACCAGCCAGGACCGGATCGTCCGCGGACGCATGCCGACGC
TGGAAATGATCAACGAGCGATTCGCCCGCTACACGCGTATCAGCATGTTCAACCTGCTGCGCCGCTCTGCGGACGTGGCG
GTGGGCGGTGTCCAGGTCATGAAGTTCGGTGAGTACGTGCATTCCCTGTACGTGCCGACCAGCCTTAACCTGGCCAAGAT
CAAGCCGCTGCGTGGCACCGCGCTGTTCATTCTCGATGCCAAGCTGGTGTTCAAGCTGGTGGACAACTTTTTCGGCGGTG
ACGGGCGTCACGCCAAGATCGAAGGCCGGGAGTTCACGCCGACCGAACTGCGTGTCGTGCGCATGGTGCTGGATCAGGCG
TTTGTCGACCTCAAGGAGGCCTGGCAGGCGATCATGGAAGTGAATTTCGAGTACATCAACTCGGAAGTGAACCCGGCCAT
GGCCAACATCGTAGGGCCGAGCGAGGCTGTAGTGATTTCCACCTTCCACATCGAACTCGATGGCGGTGGTGGCGATCTGC
ACGTGACCATGCCGTACTCGATGATCGAGCCTATCCGCGAAATGCTCGATGCCGGTTTCCAGTCCGATCTGGACGACCAG
GATGAGCGTTGGGTCAACGCGCTCAAGGAGGACGTGCTGGACGTCAACGTGCCGCTCACGACGACCATCGCCCAGCGCCA
GTTGCCGTTGCGGGACATCCTGCACATGCGCCCTGGTGATGTGATCCCGGTGGAACTGAGCGACTCGCTGGTCATGCGAG
CCAATGGCGTACCTTCATTCAAGGTCAAGCTTGGCTCGCACAAGGGCAAGATGGCCCTGCAAGTAATCGAACCCATAGCG
CGACGCTGA

Upstream 100 bases:

>100_bases
AGCGACCGCCAGCATTCAGGAAGTCGCACAGAAAGAACTCGGCAAGACTGTGATCGAACAGCTGCTCTTCACTAACTTCG
TATTGCAGTAGGACTCTCCC

Downstream 100 bases:

>100_bases
CCTTTCTGGTCTGCGTCGGAAATTGTTGCGTAACTGAAATACTGCGTGCAGAGGACAACCGATGGCTGATGAAAACGATA
TGACGTCTGCTGAAGACCAG

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MAVQDLLSQDEIDALLHGVDDGMVQTDNNSEPGSVKSYDLTSQDRIVRGRMPTLEMINERFARYTRISMFNLLRRSADVA
VGGVQVMKFGEYVHSLYVPTSLNLAKIKPLRGTALFILDAKLVFKLVDNFFGGDGRHAKIEGREFTPTELRVVRMVLDQA
FVDLKEAWQAIMEVNFEYINSEVNPAMANIVGPSEAVVISTFHIELDGGGGDLHVTMPYSMIEPIREMLDAGFQSDLDDQ
DERWVNALKEDVLDVNVPLTTTIAQRQLPLRDILHMRPGDVIPVELSDSLVMRANGVPSFKVKLGSHKGKMALQVIEPIA
RR

Sequences:

>Translated_322_residues
MAVQDLLSQDEIDALLHGVDDGMVQTDNNSEPGSVKSYDLTSQDRIVRGRMPTLEMINERFARYTRISMFNLLRRSADVA
VGGVQVMKFGEYVHSLYVPTSLNLAKIKPLRGTALFILDAKLVFKLVDNFFGGDGRHAKIEGREFTPTELRVVRMVLDQA
FVDLKEAWQAIMEVNFEYINSEVNPAMANIVGPSEAVVISTFHIELDGGGGDLHVTMPYSMIEPIREMLDAGFQSDLDDQ
DERWVNALKEDVLDVNVPLTTTIAQRQLPLRDILHMRPGDVIPVELSDSLVMRANGVPSFKVKLGSHKGKMALQVIEPIA
RR
>Mature_321_residues
AVQDLLSQDEIDALLHGVDDGMVQTDNNSEPGSVKSYDLTSQDRIVRGRMPTLEMINERFARYTRISMFNLLRRSADVAV
GGVQVMKFGEYVHSLYVPTSLNLAKIKPLRGTALFILDAKLVFKLVDNFFGGDGRHAKIEGREFTPTELRVVRMVLDQAF
VDLKEAWQAIMEVNFEYINSEVNPAMANIVGPSEAVVISTFHIELDGGGGDLHVTMPYSMIEPIREMLDAGFQSDLDDQD
ERWVNALKEDVLDVNVPLTTTIAQRQLPLRDILHMRPGDVIPVELSDSLVMRANGVPSFKVKLGSHKGKMALQVIEPIAR
R

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential). Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=322, Percent_Identity=41.6149068322981, Blast_Score=244, Evalue=4e-66,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 35995; Mature: 35864

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVQDLLSQDEIDALLHGVDDGMVQTDNNSEPGSVKSYDLTSQDRIVRGRMPTLEMINER
CCHHHHHCHHHHHHHHHCCCCCEEECCCCCCCCCCEEECCCCCCCEEECCCCHHHHHHHH
FARYTRISMFNLLRRSADVAVGGVQVMKFGEYVHSLYVPTSLNLAKIKPLRGTALFILDA
HHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHCCCCCCCHHEECCCCCCEEEEEHH
KLVFKLVDNFFGGDGRHAKIEGREFTPTELRVVRMVLDQAFVDLKEAWQAIMEVNFEYIN
HHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SEVNPAMANIVGPSEAVVISTFHIELDGGGGDLHVTMPYSMIEPIREMLDAGFQSDLDDQ
CCCCHHHHHHCCCCCEEEEEEEEEEEECCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCH
DERWVNALKEDVLDVNVPLTTTIAQRQLPLRDILHMRPGDVIPVELSDSLVMRANGVPSF
HHHHHHHHHHHHEECCCCCCHHHHHHCCCHHHHHHCCCCCEEEEECCCCEEEEECCCCEE
KVKLGSHKGKMALQVIEPIARR
EEEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
AVQDLLSQDEIDALLHGVDDGMVQTDNNSEPGSVKSYDLTSQDRIVRGRMPTLEMINER
CHHHHHCHHHHHHHHHCCCCCEEECCCCCCCCCCEEECCCCCCCEEECCCCHHHHHHHH
FARYTRISMFNLLRRSADVAVGGVQVMKFGEYVHSLYVPTSLNLAKIKPLRGTALFILDA
HHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHCCCCCCCHHEECCCCCCEEEEEHH
KLVFKLVDNFFGGDGRHAKIEGREFTPTELRVVRMVLDQAFVDLKEAWQAIMEVNFEYIN
HHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SEVNPAMANIVGPSEAVVISTFHIELDGGGGDLHVTMPYSMIEPIREMLDAGFQSDLDDQ
CCCCHHHHHHCCCCCEEEEEEEEEEEECCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCH
DERWVNALKEDVLDVNVPLTTTIAQRQLPLRDILHMRPGDVIPVELSDSLVMRANGVPSF
HHHHHHHHHHHHEECCCCCCHHHHHHCCCHHHHHHCCCCCEEEEECCCCEEEEECCCCEE
KVKLGSHKGKMALQVIEPIARR
EEEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10984043; 7622217 [H]