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The map label for this gene is fliA [H]

Identifier: 66046666

GI number: 66046666

Start: 4100576

End: 4101316

Strand: Reverse

Name: fliA [H]

Synonym: Psyr_3437

Alternate gene names: 66046666

Gene position: 4101316-4100576 (Counterclockwise)

Preceding gene: 66046667

Following gene: 66046665

Centisome position: 67.3

GC content: 58.3

Gene sequence:

>741_bases
ATGACTGCGAGCGGCTACCAGATGTACAGCAAGACTTCCCGTAATTCACAATACGAGTTGATCGAGCGCTATGCGCCCTT
GGTCAAGCGTATCGCTTATCATCTGCTGGCGCGCTTGCCGGCCAGCGTGCAGGTCGAGGATCTGATCCAGGCCGGCATGA
TCGGCCTGCTTGAAGTCTCCACCAAGTATGATTCCACCAAGGGTGCGAGTTTCGAGACCTATGCCGGCATCCGCATCCGT
GGTGCGATGCTCGATGAAGTGCGCAAGGGGGACTGGGCGCCACGCTCGGTACACCGCAATACGCGCATGGTGAGTGACGC
AATTCGGGCAATTGAAGCTAAAACCGGCCGTGACGCTAAAGATCACGAGGTTGCGGCCGAACTCCAGTTAAGTCTCGATG
ATTACTACGGGATTTTGAACGATACCTTGGGCAGCCGCCTCTTCAGTTTCGACGACCTGCTGCAGGACGGCGATCATGAA
GGGCTGCACGAGGATGGCGCAAGCGGTTCGCTTGAGCCGTCGCGCGACCTGGAAGACGAGCGCTTCCAGGCTGCGTTGGC
CGATGCAATTGCCAATCTGCCAGAGCGTGAGCGTCTGGTGTTGGCGCTGTACTACGACGAAGAGCTGAACCTCAAGGAAA
TCGGAGAGGTTCTGGGGGTCAGCGAATCTCGGGTCAGTCAGTTACACAGCCAGTGCGCAGCTCGTTTGCGAGGGCGTTTG
GGGGAGTGGCGAGCGCGTTGA

Upstream 100 bases:

>100_bases
CCCAGAAGGTTGATACCTGGCCGTTGCCAGCCAACCCGCGGGGTCATCTCGAGTTCTTCGTTGAGCGACTCGTTCACCAG
ACCAGTGCAGGGCCTGTGCA

Downstream 100 bases:

>100_bases
CAGGCAATGTGCGGTCTATGGCCGCGCGTTGACTGCAAGCCGGCTGTGCAGAGCGTTACGTGGTAACAGGATGGACCCGT
CAGAAGCCTGTCAGGCATTG

Product: flagellar biosynthesis sigma factor

Products: NA

Alternate protein names: Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MTASGYQMYSKTSRNSQYELIERYAPLVKRIAYHLLARLPASVQVEDLIQAGMIGLLEVSTKYDSTKGASFETYAGIRIR
GAMLDEVRKGDWAPRSVHRNTRMVSDAIRAIEAKTGRDAKDHEVAAELQLSLDDYYGILNDTLGSRLFSFDDLLQDGDHE
GLHEDGASGSLEPSRDLEDERFQAALADAIANLPERERLVLALYYDEELNLKEIGEVLGVSESRVSQLHSQCAARLRGRL
GEWRAR

Sequences:

>Translated_246_residues
MTASGYQMYSKTSRNSQYELIERYAPLVKRIAYHLLARLPASVQVEDLIQAGMIGLLEVSTKYDSTKGASFETYAGIRIR
GAMLDEVRKGDWAPRSVHRNTRMVSDAIRAIEAKTGRDAKDHEVAAELQLSLDDYYGILNDTLGSRLFSFDDLLQDGDHE
GLHEDGASGSLEPSRDLEDERFQAALADAIANLPERERLVLALYYDEELNLKEIGEVLGVSESRVSQLHSQCAARLRGRL
GEWRAR
>Mature_245_residues
TASGYQMYSKTSRNSQYELIERYAPLVKRIAYHLLARLPASVQVEDLIQAGMIGLLEVSTKYDSTKGASFETYAGIRIRG
AMLDEVRKGDWAPRSVHRNTRMVSDAIRAIEAKTGRDAKDHEVAAELQLSLDDYYGILNDTLGSRLFSFDDLLQDGDHEG
LHEDGASGSLEPSRDLEDERFQAALADAIANLPERERLVLALYYDEELNLKEIGEVLGVSESRVSQLHSQCAARLRGRLG
EWRAR

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellin gene (fliC) expression [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=236, Percent_Identity=50, Blast_Score=210, Evalue=6e-56,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27578; Mature: 27447

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASGYQMYSKTSRNSQYELIERYAPLVKRIAYHLLARLPASVQVEDLIQAGMIGLLEVS
CCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHH
TKYDSTKGASFETYAGIRIRGAMLDEVRKGDWAPRSVHRNTRMVSDAIRAIEAKTGRDAK
HHCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCH
DHEVAAELQLSLDDYYGILNDTLGSRLFSFDDLLQDGDHEGLHEDGASGSLEPSRDLEDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
RFQAALADAIANLPERERLVLALYYDEELNLKEIGEVLGVSESRVSQLHSQCAARLRGRL
HHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
GEWRAR
HHCCCC
>Mature Secondary Structure 
TASGYQMYSKTSRNSQYELIERYAPLVKRIAYHLLARLPASVQVEDLIQAGMIGLLEVS
CCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHH
TKYDSTKGASFETYAGIRIRGAMLDEVRKGDWAPRSVHRNTRMVSDAIRAIEAKTGRDAK
HHCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCH
DHEVAAELQLSLDDYYGILNDTLGSRLFSFDDLLQDGDHEGLHEDGASGSLEPSRDLEDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
RFQAALADAIANLPERERLVLALYYDEELNLKEIGEVLGVSESRVSQLHSQCAARLRGRL
HHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
GEWRAR
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1560774; 10984043 [H]