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The map label for this gene is alyA [H]

Identifier: 66046605

GI number: 66046605

Start: 4035018

End: 4035686

Strand: Reverse

Name: alyA [H]

Synonym: Psyr_3376

Alternate gene names: 66046605

Gene position: 4035686-4035018 (Counterclockwise)

Preceding gene: 66046613

Following gene: 66046602

Centisome position: 66.23

GC content: 56.05

Gene sequence:

>669_bases
ATGATCGACCTTGCTACCTGGAATCTCTCCGTCCCTGTCGGAAGCCCCGCGACCATTATCGAAACCCCCAAGTTGGTAAA
GGGCTATCGGGATGGCTACTTTCAGTCCGGGGATACGCTGTTTTTCTGGGCACCGGTAACGGGTTCAACCACCGAAAACG
CCAAGTACCCGCGTTCCGAATTGCGCGAGACGACTTCGGACGGAAGGGTGTTCAACTGGGCCTACTCCAGTGCCGACAAT
TTTCTGCGCGCGACCCTGGAAGTCGATCAGGTGCCTTCCAGCGGCAAGATCGTGATCGGTCAGATCCACTGCTACCAGAG
CACCGAACCGCTGCTGAAGGTGGAGTATCAGTACAAGGAGAAGCTGCAGACCGGCAATATCGTCGCCAAGTTTCGTCGTA
CGCCGGACTCGGAAATCGAGGTCATCAACATTGCGCAGGGTGTGCCGCTCAACAAGCAATTCAACTACACCATCAACCTG
TCGCCGTCTGGCGATCTGACGGTCAATGCCTTTGACGCGGTCTGGTACGCCAAGCTGGATTCAGCCTGGGCCAGCAAACT
GTTCTACTTCAAGGCAGGGGTCTATACCCAGGACAATACCGGCTACACCACGGAAGGCGGCTCGGCCACGTTCTACAAGC
TGGCCATTGCCCACGAGAAAAAGAATTGA

Upstream 100 bases:

>100_bases
AATAATCGATTAAGCCGATAGTCTGGATGACCAAACGGGCCAGCACACCCGTTTCAGAGCCATTCAGTTGGTGCGCGATG
CGCACCTTGCGAGCTACGCG

Downstream 100 bases:

>100_bases
TCCTTGCGCACTGCAGGCTTCAGGCCGCTGGGAATATCAGACTGAAGCAGATTCTGCCGACGGCGGGTTGAGCGACTTCG
ACCCGACCGCCGTGCAGTTG

Product: lyase, putative

Products: NA

Alternate protein names: Poly(beta-D-mannuronate) lyase; Poly(mana) alginate lyase [H]

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MIDLATWNLSVPVGSPATIIETPKLVKGYRDGYFQSGDTLFFWAPVTGSTTENAKYPRSELRETTSDGRVFNWAYSSADN
FLRATLEVDQVPSSGKIVIGQIHCYQSTEPLLKVEYQYKEKLQTGNIVAKFRRTPDSEIEVINIAQGVPLNKQFNYTINL
SPSGDLTVNAFDAVWYAKLDSAWASKLFYFKAGVYTQDNTGYTTEGGSATFYKLAIAHEKKN

Sequences:

>Translated_222_residues
MIDLATWNLSVPVGSPATIIETPKLVKGYRDGYFQSGDTLFFWAPVTGSTTENAKYPRSELRETTSDGRVFNWAYSSADN
FLRATLEVDQVPSSGKIVIGQIHCYQSTEPLLKVEYQYKEKLQTGNIVAKFRRTPDSEIEVINIAQGVPLNKQFNYTINL
SPSGDLTVNAFDAVWYAKLDSAWASKLFYFKAGVYTQDNTGYTTEGGSATFYKLAIAHEKKN
>Mature_222_residues
MIDLATWNLSVPVGSPATIIETPKLVKGYRDGYFQSGDTLFFWAPVTGSTTENAKYPRSELRETTSDGRVFNWAYSSADN
FLRATLEVDQVPSSGKIVIGQIHCYQSTEPLLKVEYQYKEKLQTGNIVAKFRRTPDSEIEVINIAQGVPLNKQFNYTINL
SPSGDLTVNAFDAVWYAKLDSAWASKLFYFKAGVYTQDNTGYTTEGGSATFYKLAIAHEKKN

Specific function: Degrades alginates that contain guluronic acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide lyase 7 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014895
- InterPro:   IPR008985
- InterPro:   IPR013320 [H]

Pfam domain/function: PF08787 Alginate_lyase2 [H]

EC number: =4.2.2.3 [H]

Molecular weight: Translated: 24816; Mature: 24816

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLATWNLSVPVGSPATIIETPKLVKGYRDGYFQSGDTLFFWAPVTGSTTENAKYPRSE
CEEEEEEEEECCCCCCCEEEECCHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCHHH
LRETTSDGRVFNWAYSSADNFLRATLEVDQVPSSGKIVIGQIHCYQSTEPLLKVEYQYKE
HHHCCCCCCEEEEEECCCCCEEEEEEEEECCCCCCCEEEEEEEEECCCCCEEEEEEHHHH
KLQTGNIVAKFRRTPDSEIEVINIAQGVPLNKQFNYTINLSPSGDLTVNAFDAVWYAKLD
CCCCCCEEEEEECCCCCCEEEEEECCCCCCCCEEEEEEEECCCCCEEEEEEHEEEEEHHH
SAWASKLFYFKAGVYTQDNTGYTTEGGSATFYKLAIAHEKKN
HHHHHEEEEEECCEEEECCCCEEECCCCEEEEEEEEEECCCC
>Mature Secondary Structure
MIDLATWNLSVPVGSPATIIETPKLVKGYRDGYFQSGDTLFFWAPVTGSTTENAKYPRSE
CEEEEEEEEECCCCCCCEEEECCHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCHHH
LRETTSDGRVFNWAYSSADNFLRATLEVDQVPSSGKIVIGQIHCYQSTEPLLKVEYQYKE
HHHCCCCCCEEEEEECCCCCEEEEEEEEECCCCCCCEEEEEEEEECCCCCEEEEEEHHHH
KLQTGNIVAKFRRTPDSEIEVINIAQGVPLNKQFNYTINLSPSGDLTVNAFDAVWYAKLD
CCCCCCEEEEEECCCCCCEEEEEECCCCCCCCEEEEEEEECCCCCEEEEEEHEEEEEHHH
SAWASKLFYFKAGVYTQDNTGYTTEGGSATFYKLAIAHEKKN
HHHHHEEEEEECCEEEECCCCEEECCCCEEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8200539 [H]