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The map label for this gene is iolD [H]

Identifier: 66046498

GI number: 66046498

Start: 3914548

End: 3916485

Strand: Reverse

Name: iolD [H]

Synonym: Psyr_3269

Alternate gene names: 66046498

Gene position: 3916485-3914548 (Counterclockwise)

Preceding gene: 66046499

Following gene: 66046497

Centisome position: 64.27

GC content: 60.84

Gene sequence:

>1938_bases
ATGAGTACAACCCGACTGACCATGGCCCAGGCCCTGGTGAAGTTTCTCGATAACCAGTACGTCGAAGTCGATGGCGTACA
GAGCAAATTCGTCGCTGGCATCTTCACCATTTTCGGGCATGGCAACGTGCTGGGGCTTGGGCAGGCGCTGGAGCAGGACA
GCGGCGACCTGGTGGTCCATCAGGGCCGTAACGAGCAGGGCATGTGCCACGCCGCCATCGGTTTTGCCAAGCAGCACCTG
CGTCGCAAGATCTACGCGTGCAGTTCATCGGTCGGCCCTGGCGCGGCGAACATGGTCACCGCTGCGGCAACGGCCTCGGC
CAACCGCATTCCGTTGCTGCTGTTGCCGGGCGATGTCTACGCCAGCCGCCAGCCCGATCCGGTGCTGCAGCAGATCGAGC
AGTTTCACGACCTGAGCATCAGCACCAACGACGCGTTCAAGGCGGTGAGCAAATACTGGGACCGTATCAACCGTCCCGAG
CAGTTGATGAGCGCTGCGCTCAATGCCATGCGTGTACTGACCGACCCGGCCGATACGGGTGCCGTTACCCTGGCGCTGCC
TCAGGATGTGCAGGCTGAAGCCTACGACTACCCGGACAGCTTTCTGCAAAAACGCGTGCACCGTATCGACCGGCGTCCGC
CAAGCAAGGCCATGCTTGACGACGCGTTGCAACTACTTGTCGGCAAGCGCAAGCCGTTGCTGATCTGCGGCGGCGGGGTG
CGTTACTCCGGTGCGGCGGATGCCTTGCAAGCCTTTGCCGAGCGCTTCGATATCCCGTTCGCCGAAACCCAGGCCGGCAA
GAGTGCCATCGTTTCGGCGCACCCGTTGAACATGGGCGGCATCGGCGAAACCGGCACGCTGGCAGCCAATCGGCTGGCCA
AAGAGGCTGACCTGATCATCGGCGTCGGCACCCGTTACAGCGATTTCACCACCGCGTCGAAATGGCTGTTCCAGAACCCG
GATGTGCAGTTTCTCAACCTCAATGTCGGTGCCTTCGATGTGCAGAAACTGGACGGTGTGCAGGTGCTGGCTGATGCGCA
GACGGCATTGCAGGCGCTGACCGAAAGCCTGCAAGCCAGAGGTTATCGTGCGGCCTGGGGCGATGCACCGCAATCGGCAC
GTGCCGAGCTGCATGCCGAGGTGGATCGCCTGTACGCGGTCGAGTACCAGAACGAGGATTTTGTCCCGGAAATCAACGAT
CACCTGGACCCTGCGGTGCTGCGCGAGTTCATCGAGCTGACCGGTTCGTGCCTGACTCAGAGTGGTGTGTTGGGCATCCT
CAACCAGAGCCTGCCTGCCGATGCGGTGATCGTTGCAGCAGCGGGCAGCTTGCCGGGTGATCTGCAGCGCGCCTGGCGCA
GTACCGGAGTAGATACCTATCACGTCGAATACGGTTATTCGTGCATGGGTTATGAGGTCAACGCCGCACTGGGCGTGAAG
CTTGCGGCGCCGCATCGGGAAGTGTTTGCACTGGTGGGTGACGGCTCTTACATGATGCTTCATTCCGAGCTGGCGACCTC
CATCCAGGAGCGGCGCAAGATCAACGTTGTGTTGCTGGACAATATGACCTTCGGCTGCATCAACAACCTGCAGATGGAGC
ACGGCATGAACAGCTTCGGCACTGAGTTCCGTTTCCGCAATCCGGACACAGGCAGGCTCGACGGTGACTTCGTGCCGGTG
GATTTCGCCATGAGTGCTGCTGCCTATGGCTGCAAGACTTACAAGGTGAATACGGCCGAACAACTGCGCAACGCACTGGC
CGATGCGCAACGTCAGACGGTGTCGACGCTGATCGACATCAAGGTCCTGCCCAAGACCATGATCCACAAATACCTGTCGT
GGTGGCGTGTCGGGGTTGCCGAAGTGTCGACCACCGGCACCACCGCTCAGGTGTACGAGAAGCTCAACCGCGAGCTGGCC
AAGGCTCGCCAGTACTGA

Upstream 100 bases:

>100_bases
TCAGGCAATAGCGGCAAGCATGACGCATTTGTCACGAGCCGTTTCGCAGCGGCACTGACTTCAGTTGTCAGGCCACCTCA
AACAAGAACAAGGTGCGAAC

Downstream 100 bases:

>100_bases
TCACTTTTATCGTTCAGCGCTCGTGAACCGTTCACGGGTATATCCAATAACAAAAAAGGAACATGAAAATGGCATTGAAA
CTCGGCGTGATCGGTACAGG

Product: pyruvate decarboxylase:pyruvate decarboxylase

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase [H]

Number of amino acids: Translated: 645; Mature: 644

Protein sequence:

>645_residues
MSTTRLTMAQALVKFLDNQYVEVDGVQSKFVAGIFTIFGHGNVLGLGQALEQDSGDLVVHQGRNEQGMCHAAIGFAKQHL
RRKIYACSSSVGPGAANMVTAAATASANRIPLLLLPGDVYASRQPDPVLQQIEQFHDLSISTNDAFKAVSKYWDRINRPE
QLMSAALNAMRVLTDPADTGAVTLALPQDVQAEAYDYPDSFLQKRVHRIDRRPPSKAMLDDALQLLVGKRKPLLICGGGV
RYSGAADALQAFAERFDIPFAETQAGKSAIVSAHPLNMGGIGETGTLAANRLAKEADLIIGVGTRYSDFTTASKWLFQNP
DVQFLNLNVGAFDVQKLDGVQVLADAQTALQALTESLQARGYRAAWGDAPQSARAELHAEVDRLYAVEYQNEDFVPEIND
HLDPAVLREFIELTGSCLTQSGVLGILNQSLPADAVIVAAAGSLPGDLQRAWRSTGVDTYHVEYGYSCMGYEVNAALGVK
LAAPHREVFALVGDGSYMMLHSELATSIQERRKINVVLLDNMTFGCINNLQMEHGMNSFGTEFRFRNPDTGRLDGDFVPV
DFAMSAAAYGCKTYKVNTAEQLRNALADAQRQTVSTLIDIKVLPKTMIHKYLSWWRVGVAEVSTTGTTAQVYEKLNRELA
KARQY

Sequences:

>Translated_645_residues
MSTTRLTMAQALVKFLDNQYVEVDGVQSKFVAGIFTIFGHGNVLGLGQALEQDSGDLVVHQGRNEQGMCHAAIGFAKQHL
RRKIYACSSSVGPGAANMVTAAATASANRIPLLLLPGDVYASRQPDPVLQQIEQFHDLSISTNDAFKAVSKYWDRINRPE
QLMSAALNAMRVLTDPADTGAVTLALPQDVQAEAYDYPDSFLQKRVHRIDRRPPSKAMLDDALQLLVGKRKPLLICGGGV
RYSGAADALQAFAERFDIPFAETQAGKSAIVSAHPLNMGGIGETGTLAANRLAKEADLIIGVGTRYSDFTTASKWLFQNP
DVQFLNLNVGAFDVQKLDGVQVLADAQTALQALTESLQARGYRAAWGDAPQSARAELHAEVDRLYAVEYQNEDFVPEIND
HLDPAVLREFIELTGSCLTQSGVLGILNQSLPADAVIVAAAGSLPGDLQRAWRSTGVDTYHVEYGYSCMGYEVNAALGVK
LAAPHREVFALVGDGSYMMLHSELATSIQERRKINVVLLDNMTFGCINNLQMEHGMNSFGTEFRFRNPDTGRLDGDFVPV
DFAMSAAAYGCKTYKVNTAEQLRNALADAQRQTVSTLIDIKVLPKTMIHKYLSWWRVGVAEVSTTGTTAQVYEKLNRELA
KARQY
>Mature_644_residues
STTRLTMAQALVKFLDNQYVEVDGVQSKFVAGIFTIFGHGNVLGLGQALEQDSGDLVVHQGRNEQGMCHAAIGFAKQHLR
RKIYACSSSVGPGAANMVTAAATASANRIPLLLLPGDVYASRQPDPVLQQIEQFHDLSISTNDAFKAVSKYWDRINRPEQ
LMSAALNAMRVLTDPADTGAVTLALPQDVQAEAYDYPDSFLQKRVHRIDRRPPSKAMLDDALQLLVGKRKPLLICGGGVR
YSGAADALQAFAERFDIPFAETQAGKSAIVSAHPLNMGGIGETGTLAANRLAKEADLIIGVGTRYSDFTTASKWLFQNPD
VQFLNLNVGAFDVQKLDGVQVLADAQTALQALTESLQARGYRAAWGDAPQSARAELHAEVDRLYAVEYQNEDFVPEINDH
LDPAVLREFIELTGSCLTQSGVLGILNQSLPADAVIVAAAGSLPGDLQRAWRSTGVDTYHVEYGYSCMGYEVNAALGVKL
AAPHREVFALVGDGSYMMLHSELATSIQERRKINVVLLDNMTFGCINNLQMEHGMNSFGTEFRFRNPDTGRLDGDFVPVD
FAMSAAAYGCKTYKVNTAEQLRNALADAQRQTVSTLIDIKVLPKTMIHKYLSWWRVGVAEVSTTGTTAQVYEKLNRELAK
ARQY

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=569, Percent_Identity=20.9138840070299, Blast_Score=80, Evalue=8e-15,
Organism=Escherichia coli, GI1790104, Length=610, Percent_Identity=24.5901639344262, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI87081685, Length=584, Percent_Identity=25.8561643835616, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1786717, Length=540, Percent_Identity=25.5555555555556, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1788716, Length=302, Percent_Identity=25.8278145695364, Blast_Score=76, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6323755, Length=591, Percent_Identity=23.1810490693739, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19922626, Length=574, Percent_Identity=22.2996515679443, Blast_Score=80, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 70359; Mature: 70227

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTRLTMAQALVKFLDNQYVEVDGVQSKFVAGIFTIFGHGNVLGLGQALEQDSGDLVVH
CCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCEEECCHHHCCCCCCEEEE
QGRNEQGMCHAAIGFAKQHLRRKIYACSSSVGPGAANMVTAAATASANRIPLLLLPGDVY
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCC
ASRQPDPVLQQIEQFHDLSISTNDAFKAVSKYWDRINRPEQLMSAALNAMRVLTDPADTG
CCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
AVTLALPQDVQAEAYDYPDSFLQKRVHRIDRRPPSKAMLDDALQLLVGKRKPLLICGGGV
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEECCCC
RYSGAADALQAFAERFDIPFAETQAGKSAIVSAHPLNMGGIGETGTLAANRLAKEADLII
CCCCHHHHHHHHHHHHCCCCHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCEEE
GVGTRYSDFTTASKWLFQNPDVQFLNLNVGAFDVQKLDGVQVLADAQTALQALTESLQAR
ECCCCCCCCHHHHHHHCCCCCEEEEEECCCEEEHHHCCCHHHHHHHHHHHHHHHHHHHHC
GYRAAWGDAPQSARAELHAEVDRLYAVEYQNEDFVPEINDHLDPAVLREFIELTGSCLTQ
CCCCCCCCCCHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
SGVLGILNQSLPADAVIVAAAGSLPGDLQRAWRSTGVDTYHVEYGYSCMGYEVNAALGVK
CCHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCEEECEEECCEEEEE
LAAPHREVFALVGDGSYMMLHSELATSIQERRKINVVLLDNMTFGCINNLQMEHGMNSFG
EECCCCEEEEEECCCCEEEEHHHHHHHHHHHCCEEEEEECCCCHHHHHCCCHHCCCCHHC
TEFRFRNPDTGRLDGDFVPVDFAMSAAAYGCKTYKVNTAEQLRNALADAQRQTVSTLIDI
CCEEECCCCCCCCCCCEEEHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHH
KVLPKTMIHKYLSWWRVGVAEVSTTGTTAQVYEKLNRELAKARQY
HHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STTRLTMAQALVKFLDNQYVEVDGVQSKFVAGIFTIFGHGNVLGLGQALEQDSGDLVVH
CCHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCEEECCHHHCCCCCCEEEE
QGRNEQGMCHAAIGFAKQHLRRKIYACSSSVGPGAANMVTAAATASANRIPLLLLPGDVY
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCC
ASRQPDPVLQQIEQFHDLSISTNDAFKAVSKYWDRINRPEQLMSAALNAMRVLTDPADTG
CCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
AVTLALPQDVQAEAYDYPDSFLQKRVHRIDRRPPSKAMLDDALQLLVGKRKPLLICGGGV
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEECCCC
RYSGAADALQAFAERFDIPFAETQAGKSAIVSAHPLNMGGIGETGTLAANRLAKEADLII
CCCCHHHHHHHHHHHHCCCCHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCEEE
GVGTRYSDFTTASKWLFQNPDVQFLNLNVGAFDVQKLDGVQVLADAQTALQALTESLQAR
ECCCCCCCCHHHHHHHCCCCCEEEEEECCCEEEHHHCCCHHHHHHHHHHHHHHHHHHHHC
GYRAAWGDAPQSARAELHAEVDRLYAVEYQNEDFVPEINDHLDPAVLREFIELTGSCLTQ
CCCCCCCCCCHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
SGVLGILNQSLPADAVIVAAAGSLPGDLQRAWRSTGVDTYHVEYGYSCMGYEVNAALGVK
CCHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCEEECEEECCEEEEE
LAAPHREVFALVGDGSYMMLHSELATSIQERRKINVVLLDNMTFGCINNLQMEHGMNSFG
EECCCCEEEEEECCCCEEEEHHHHHHHHHHHCCEEEEEECCCCHHHHHCCCHHCCCCHHC
TEFRFRNPDTGRLDGDFVPVDFAMSAAAYGCKTYKVNTAEQLRNALADAQRQTVSTLIDI
CCEEECCCCCCCCCCCEEEHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHH
KVLPKTMIHKYLSWWRVGVAEVSTTGTTAQVYEKLNRELAKARQY
HHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; Decarboxylation [C]

Inhibitor: 2-oxobutanoate; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA