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The map label for this gene is rfaH [H]

Identifier: 66046455

GI number: 66046455

Start: 3864736

End: 3865248

Strand: Direct

Name: rfaH [H]

Synonym: Psyr_3226

Alternate gene names: 66046455

Gene position: 3864736-3865248 (Clockwise)

Preceding gene: 66046454

Following gene: 66046456

Centisome position: 63.42

GC content: 59.26

Gene sequence:

>513_bases
ATGTCCCACTCCGACCAGGATGTCCGCTGGTACCTGATCCAGACCAAACCACGGCAGGAAGCCCGCGCCGAAGAACATTT
GCGGCGTCAGCAGTTCGAGTGCTATCGACCGTTACAGGCCGATGCAAAAAAAACCGGTACCAGGGTTCAGGCCGGCGAGC
CCTTGTTCCCCGGCTATCTGTTCATTCACATGGATCAGGTGCAAGACAACTGGTACCCGATTCGCTCCACCCGCGGCGTC
TCGCGCATCGTGACCTTCGGTGGTCATCCGGTGCCGGTTCGGGATCATCTGATCGAGCAGATCCGCCAACGACTGGCCAC
GCCCGCACCGAAAACTCAGTTTGCTCAAGGCGACGCGGTGCTGATCACGCATGCCAGTTGGGGTGACGTCGAGGCCATTT
TTCTGAGCAAAGACGGCACGCAGCGGGCGGTTATTCTGCTCAATATGTTGCAGCGCCAGCAAAAAGTGGTGCTTCCCATC
AGCAGCCTGTCGCGAATCGAAGCCCGCGCCTAG

Upstream 100 bases:

>100_bases
TACGACTGCGCCGCCTACGGCTACAACTACCACCCCGCGCCCCGATAGAAGGGACGCTGCCTGCCGCTGACCGAGCTGTT
TCATTTAAAAGGAATTAATC

Downstream 100 bases:

>100_bases
CGCTGGAATCCGCAGAAACCTGCCGCACGACGTGTGCCTGAAAACATGTGTCGCATGCCTACTCCACCAGCCTCGGAACC
ACACCATGCCGCGCCAGACC

Product: transcriptional activator RfaH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 170; Mature: 169

Protein sequence:

>170_residues
MSHSDQDVRWYLIQTKPRQEARAEEHLRRQQFECYRPLQADAKKTGTRVQAGEPLFPGYLFIHMDQVQDNWYPIRSTRGV
SRIVTFGGHPVPVRDHLIEQIRQRLATPAPKTQFAQGDAVLITHASWGDVEAIFLSKDGTQRAVILLNMLQRQQKVVLPI
SSLSRIEARA

Sequences:

>Translated_170_residues
MSHSDQDVRWYLIQTKPRQEARAEEHLRRQQFECYRPLQADAKKTGTRVQAGEPLFPGYLFIHMDQVQDNWYPIRSTRGV
SRIVTFGGHPVPVRDHLIEQIRQRLATPAPKTQFAQGDAVLITHASWGDVEAIFLSKDGTQRAVILLNMLQRQQKVVLPI
SSLSRIEARA
>Mature_169_residues
SHSDQDVRWYLIQTKPRQEARAEEHLRRQQFECYRPLQADAKKTGTRVQAGEPLFPGYLFIHMDQVQDNWYPIRSTRGVS
RIVTFGGHPVPVRDHLIEQIRQRLATPAPKTQFAQGDAVLITHASWGDVEAIFLSKDGTQRAVILLNMLQRQQKVVLPIS
SLSRIEARA

Specific function: Transcriptional activator of hemolysin synthesis and secretion, also required for expression of sex factor and lipopolysaccharide biosynthesis genes. Transcriptional activator of rfaD/htrM and htrL [H]

COG id: COG0250

COG function: function code K; Transcription antiterminator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790276, Length=154, Percent_Identity=37.6623376623377, Blast_Score=97, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005824
- InterPro:   IPR010215
- InterPro:   IPR006645
- InterPro:   IPR014722 [H]

Pfam domain/function: PF02357 NusG [H]

EC number: NA

Molecular weight: Translated: 19524; Mature: 19392

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHSDQDVRWYLIQTKPRQEARAEEHLRRQQFECYRPLQADAKKTGTRVQAGEPLFPGYL
CCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCEE
FIHMDQVQDNWYPIRSTRGVSRIVTFGGHPVPVRDHLIEQIRQRLATPAPKTQFAQGDAV
EEEEECCCCCCEECCCCCCHHHEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEE
LITHASWGDVEAIFLSKDGTQRAVILLNMLQRQQKVVLPISSLSRIEARA
EEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHCEEEEEHHHHHHHHCCC
>Mature Secondary Structure 
SHSDQDVRWYLIQTKPRQEARAEEHLRRQQFECYRPLQADAKKTGTRVQAGEPLFPGYL
CCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCEE
FIHMDQVQDNWYPIRSTRGVSRIVTFGGHPVPVRDHLIEQIRQRLATPAPKTQFAQGDAV
EEEEECCCCCCEECCCCCCHHHEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEE
LITHASWGDVEAIFLSKDGTQRAVILLNMLQRQQKVVLPISSLSRIEARA
EEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHCEEEEEHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]