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The map label for this gene is aceA [H]

Identifier: 66046425

GI number: 66046425

Start: 3825917

End: 3827242

Strand: Direct

Name: aceA [H]

Synonym: Psyr_3196

Alternate gene names: 66046425

Gene position: 3825917-3827242 (Clockwise)

Preceding gene: 66046424

Following gene: 66046426

Centisome position: 62.78

GC content: 60.41

Gene sequence:

>1326_bases
ATGGCACTGACACGCGAACAACAAATAGCGGCTCTCGAGAAAGACTGGGCTGAAAATCCGCGCTGGACAAACGTGACCCG
TACTTACTCCGCTGCAGATGTGGTCCGCCTGCGCGGCTCAGTTCAACCCGAACACACCTTTGCACGACTGGGTGCAGACA
AGCTGTGGAATCTGGTGACCCAGGGTGCCAAACCGTCCTTCCGGCCGGACAAGGATTTCGTCAATTGCATGGGCGCCCTC
ACCGGTGGCCAGGCCGTGCAGCAGGTCAAGGCCGGCATTCAGGCCATCTACCTGTCCGGCTGGCAGGTCGCTGCCGACAA
CAACTCTGCCGAGTCCATGTACCCGGATCAGTCGCTGTACCCGGTCGACTCGGTGCCGACCGTGGTCAAGCGCATCAACA
ACTCGTTCCGCCGTGCCGACCAGATCCAGTGGAAAGCCGGCAAGGCGCCGGGTGACGAAGGCTACATCGACTACTTCGCT
CCGATCGTCGCAGATGCGGAAGCCGGATTCGGCGGCGTACTCAATGCCTATGAGCTGATGAAAAGCATGATCGAAGCCGG
CGCCGCCGGGGTGCACTTCGAAGATCAACTGGCGTCGGTGAAGAAATGCGGCCACATGGGCGGCAAGGTACTGGTCCCGA
CTCAGGAAGCCATACAGAAACTGGTCGCTGCACGCCTGGCCGCCGACGTGGCCGGTGTACCGACCATCATTCTGGCCCGG
ACTGACGCCAACGCTGCCGACTTGCTGACATCCGATTGCGATCCGTACGACAAGCCATTCGTGACCGGCGAACGAACCCA
TGAGGGGTTCTACAAGGTTCGCGCCGGCCTCGATCAGGCCATCGCGCGCGGCCTGGCGTACGCGCCTTACGCTGATCTGA
TCTGGTGCGAAACAGCCAGGCCGGACCTCGACGAGGCACGCCGCTTCGCCGAAGCGATCAAAAAGCAATACCCCGATCAG
TTGCTGTCCTACAACTGCTCGCCGTCCTTCAACTGGAAGAAAAACCTCGACGACGCAACCATCGCCAGGTTTCAGCGCGA
GCTCTCGGCCATGGGCTACAAGCACCAGTTCATCACCCTGGCGGGCATTCACAACATGTGGCACAGCATGTTCAACCTGG
CGCACGACTACGCCCGCAATGACATGACCGCCTATGTGAAGCTGCAGGAACAGGAGTTCGCCGACGCTGCCAAGGGCTAC
ACCTTCGTGGCTCACCAGCAGGAAGTGGGCACGGGCTATTTCGATGACATGACGACCGTGATCCAGGGTGGCGTATCGTC
GGTAACCGCACTGACCGGCTCAACCGAAGAAGAGCAATTCCACTGA

Upstream 100 bases:

>100_bases
CCCCGGATCAGCGAGCAGCCATCTACCGTGCCTTCGATAAACGAGGCGTTTTGGCGGAGCAGGACTTTCCAGCGAGACCT
ACTTCTAGAGGTTTATCACC

Downstream 100 bases:

>100_bases
CACACCGCGCAACGCGTTGAAAATACAGGCCGTCCGTAAGGGCGGTCTGTCTGTTGTGTCAGAGGGTTCCGGTATTGACT
TCGGACAATATGCAGTTGCC

Product: isocitrate lyase

Products: NA

Alternate protein names: ICL; Isocitrase; Isocitratase [H]

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MALTREQQIAALEKDWAENPRWTNVTRTYSAADVVRLRGSVQPEHTFARLGADKLWNLVTQGAKPSFRPDKDFVNCMGAL
TGGQAVQQVKAGIQAIYLSGWQVAADNNSAESMYPDQSLYPVDSVPTVVKRINNSFRRADQIQWKAGKAPGDEGYIDYFA
PIVADAEAGFGGVLNAYELMKSMIEAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARLAADVAGVPTIILAR
TDANAADLLTSDCDPYDKPFVTGERTHEGFYKVRAGLDQAIARGLAYAPYADLIWCETARPDLDEARRFAEAIKKQYPDQ
LLSYNCSPSFNWKKNLDDATIARFQRELSAMGYKHQFITLAGIHNMWHSMFNLAHDYARNDMTAYVKLQEQEFADAAKGY
TFVAHQQEVGTGYFDDMTTVIQGGVSSVTALTGSTEEEQFH

Sequences:

>Translated_441_residues
MALTREQQIAALEKDWAENPRWTNVTRTYSAADVVRLRGSVQPEHTFARLGADKLWNLVTQGAKPSFRPDKDFVNCMGAL
TGGQAVQQVKAGIQAIYLSGWQVAADNNSAESMYPDQSLYPVDSVPTVVKRINNSFRRADQIQWKAGKAPGDEGYIDYFA
PIVADAEAGFGGVLNAYELMKSMIEAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARLAADVAGVPTIILAR
TDANAADLLTSDCDPYDKPFVTGERTHEGFYKVRAGLDQAIARGLAYAPYADLIWCETARPDLDEARRFAEAIKKQYPDQ
LLSYNCSPSFNWKKNLDDATIARFQRELSAMGYKHQFITLAGIHNMWHSMFNLAHDYARNDMTAYVKLQEQEFADAAKGY
TFVAHQQEVGTGYFDDMTTVIQGGVSSVTALTGSTEEEQFH
>Mature_440_residues
ALTREQQIAALEKDWAENPRWTNVTRTYSAADVVRLRGSVQPEHTFARLGADKLWNLVTQGAKPSFRPDKDFVNCMGALT
GGQAVQQVKAGIQAIYLSGWQVAADNNSAESMYPDQSLYPVDSVPTVVKRINNSFRRADQIQWKAGKAPGDEGYIDYFAP
IVADAEAGFGGVLNAYELMKSMIEAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARLAADVAGVPTIILART
DANAADLLTSDCDPYDKPFVTGERTHEGFYKVRAGLDQAIARGLAYAPYADLIWCETARPDLDEARRFAEAIKKQYPDQL
LSYNCSPSFNWKKNLDDATIARFQRELSAMGYKHQFITLAGIHNMWHSMFNLAHDYARNDMTAYVKLQEQEFADAAKGYT
FVAHQQEVGTGYFDDMTTVIQGGVSSVTALTGSTEEEQFH

Specific function: Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase- positive serine pathway [H]

COG id: COG2224

COG function: function code C; Isocitrate lyase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1790445, Length=439, Percent_Identity=72.6651480637813, Blast_Score=665, Evalue=0.0,
Organism=Escherichia coli, GI1786525, Length=279, Percent_Identity=29.0322580645161, Blast_Score=89, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17561814, Length=427, Percent_Identity=57.8454332552693, Blast_Score=491, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI71982926, Length=427, Percent_Identity=57.8454332552693, Blast_Score=491, Evalue=1e-139,
Organism=Saccharomyces cerevisiae, GI6320908, Length=329, Percent_Identity=34.3465045592705, Blast_Score=169, Evalue=7e-43,
Organism=Saccharomyces cerevisiae, GI6325263, Length=254, Percent_Identity=34.251968503937, Blast_Score=154, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006254
- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.1 [H]

Molecular weight: Translated: 48635; Mature: 48504

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTREQQIAALEKDWAENPRWTNVTRTYSAADVVRLRGSVQPEHTFARLGADKLWNLVT
CCCCCHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHCCCCCHHHHHHHCHHHHHHHHH
QGAKPSFRPDKDFVNCMGALTGGQAVQQVKAGIQAIYLSGWQVAADNNSAESMYPDQSLY
CCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHEEEECCEEEEECCCCCHHCCCCCCCC
PVDSVPTVVKRINNSFRRADQIQWKAGKAPGDEGYIDYFAPIVADAEAGFGGVLNAYELM
CCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHH
KSMIEAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARLAADVAGVPTIILAR
HHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEE
TDANAADLLTSDCDPYDKPFVTGERTHEGFYKVRAGLDQAIARGLAYAPYADLIWCETAR
CCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHEEEECCCC
PDLDEARRFAEAIKKQYPDQLLSYNCSPSFNWKKNLDDATIARFQRELSAMGYKHQFITL
CCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHEEEH
AGIHNMWHSMFNLAHDYARNDMTAYVKLQEQEFADAAKGYTFVAHQQEVGTGYFDDMTTV
HHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHCCCEEEEEHHHHCCCHHHHHHHH
IQGGVSSVTALTGSTEEEQFH
HHCCHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
ALTREQQIAALEKDWAENPRWTNVTRTYSAADVVRLRGSVQPEHTFARLGADKLWNLVT
CCCCHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHCCCCCHHHHHHHCHHHHHHHHH
QGAKPSFRPDKDFVNCMGALTGGQAVQQVKAGIQAIYLSGWQVAADNNSAESMYPDQSLY
CCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHEEEECCEEEEECCCCCHHCCCCCCCC
PVDSVPTVVKRINNSFRRADQIQWKAGKAPGDEGYIDYFAPIVADAEAGFGGVLNAYELM
CCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHH
KSMIEAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARLAADVAGVPTIILAR
HHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEE
TDANAADLLTSDCDPYDKPFVTGERTHEGFYKVRAGLDQAIARGLAYAPYADLIWCETAR
CCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHEEEECCCC
PDLDEARRFAEAIKKQYPDQLLSYNCSPSFNWKKNLDDATIARFQRELSAMGYKHQFITL
CCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHEEEH
AGIHNMWHSMFNLAHDYARNDMTAYVKLQEQEFADAAKGYTFVAHQQEVGTGYFDDMTTV
HHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHCCCEEEEEHHHHCCCHHHHHHHH
IQGGVSSVTALTGSTEEEQFH
HHCCHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]