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The map label for this gene is aprF [H]

Identifier: 66046388

GI number: 66046388

Start: 3786200

End: 3787549

Strand: Reverse

Name: aprF [H]

Synonym: Psyr_3159

Alternate gene names: 66046388

Gene position: 3787549-3786200 (Counterclockwise)

Preceding gene: 66046389

Following gene: 66046384

Centisome position: 62.16

GC content: 60.81

Gene sequence:

>1350_bases
ATGTCTCGTCATCCCTTTAAAGCCGCTCTGCTGACGGCCATCCTGCTGGGTACTGCACCTTTGAGCCAGGCCATGGGCCC
GTTTCAGGTCTATGAATTGGCGCTTCGCAATGATCCGGTTTATCTGGGCGCACTCAAGGAGCGCGACGCGGGCCTGGAGA
ACCGGGTGATTGGTCGTGCCGGCTTGTTGCCTCGCGTGTCATACAGCTACAACAAGGGGCGCAACGATTCCAAGGCGACC
CTCAAAGGCAGCGCTCAAGACACCACCGACCATCGCCACTACAACAGCTTTGGCTCGAGCCTGACGGTTCAGCAGCCCTT
GATCGATTACGAGGCGTACGCCAATTACCGCAAGGGGCTGGCTCAGGCGCTGTTTGCCGACGAGACCTTTCGCAGCAAGA
GCCAGGAATTGCTGGTGCGGGTGTTGACCCTGTACACCCAGGCACTTTTCGCCCAGGACCAGATCAATATCGCCCGTGCC
AAGCAGCAGGCGTTCGAGCGCCAGTTCGCGCAGAACCGGCAGATGTTCGAGCGGGGAGAAGGCACGCGCACCGATATTCT
CGAAGCCGAATCGCGCAACGAACTGGCGATTGCCGAGCAGATCGAAGCCAGCGATGAGCAGGATGCGGCCTTGCGCGAGC
TGGCGGCGTTGTTGGGCGAACCGAGTATCGATGTTGCGCAGCTGGAGCCATTGAGGGACAGCTTTCAAGCGTTGGTACTG
GCACCGTCCAGTTTTACCGAGTGGCATGCGCTGGCCATGGCCAACAGCCCGATCCTGGCCTCGCAACGGCAGTCCCTGGA
AGTGGCGCGATACGAGGTGGAACGCAACCGCGCAGGGCATCTGCCGACGGTGAACGCCTACGCAAGTATCCGCCAGAACG
AATCGGACAGCGGCAACACCTACAATCAGCGTTACGACACCAACACGATAGGCATCGAGCTCAGTCTTCCGTTGTATGCC
GGAGGTGGCACCAGCGCCTCGGTGCGCCAGGCCAACAGCAAGCGCGAGCAGGCTGAATACGAGCTGGAGGGCAAGACCCG
GGAAACCCTGATCGAACTGCGTCGCCAGTTCAACGCCTGTGCGTCAGGGGTGAACAAGTTGCGCGCCTACCAGAAAGCGC
TGATCTCGGCGCAGGCACTGGTCGAGTCGACGCGCCAGAGCATTCTGGGTGGCGAGCGCATCAGCCTGGATGCCCTTAAC
GCCGAGCAGCAGCTCTACAGTACCCGTCGCGATCTGGCAAAAGCCCGTTACGACTATTTGATGGCCTGGATCAAACTGCA
TTACTACGCCGGCACCTTGCGCGACACCGATCTGGCGCGCATCGACGAGGCGTTCGTGGTGGCGCGCTGA

Upstream 100 bases:

>100_bases
CCCGCTGAAATGTTCGTGCGCACGGGTGAGCGCTCGCTGCTCAATTATCTGTTCAAACCTTTGCTGGACCGTGCCGGCTC
CGCGTTGACGGAGGAGTAGG

Downstream 100 bases:

>100_bases
TCAAGCCTGCCTGGCGAAGCGTTGCAATGAAAAGGCCGAGAGATCCACATCGCTCTCGCCATCAATACAACGTTGCGCCA
GCGCCAGTCCAAGCCCGGCC

Product: Type I secretion outer membrane protein, TolC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MSRHPFKAALLTAILLGTAPLSQAMGPFQVYELALRNDPVYLGALKERDAGLENRVIGRAGLLPRVSYSYNKGRNDSKAT
LKGSAQDTTDHRHYNSFGSSLTVQQPLIDYEAYANYRKGLAQALFADETFRSKSQELLVRVLTLYTQALFAQDQINIARA
KQQAFERQFAQNRQMFERGEGTRTDILEAESRNELAIAEQIEASDEQDAALRELAALLGEPSIDVAQLEPLRDSFQALVL
APSSFTEWHALAMANSPILASQRQSLEVARYEVERNRAGHLPTVNAYASIRQNESDSGNTYNQRYDTNTIGIELSLPLYA
GGGTSASVRQANSKREQAEYELEGKTRETLIELRRQFNACASGVNKLRAYQKALISAQALVESTRQSILGGERISLDALN
AEQQLYSTRRDLAKARYDYLMAWIKLHYYAGTLRDTDLARIDEAFVVAR

Sequences:

>Translated_449_residues
MSRHPFKAALLTAILLGTAPLSQAMGPFQVYELALRNDPVYLGALKERDAGLENRVIGRAGLLPRVSYSYNKGRNDSKAT
LKGSAQDTTDHRHYNSFGSSLTVQQPLIDYEAYANYRKGLAQALFADETFRSKSQELLVRVLTLYTQALFAQDQINIARA
KQQAFERQFAQNRQMFERGEGTRTDILEAESRNELAIAEQIEASDEQDAALRELAALLGEPSIDVAQLEPLRDSFQALVL
APSSFTEWHALAMANSPILASQRQSLEVARYEVERNRAGHLPTVNAYASIRQNESDSGNTYNQRYDTNTIGIELSLPLYA
GGGTSASVRQANSKREQAEYELEGKTRETLIELRRQFNACASGVNKLRAYQKALISAQALVESTRQSILGGERISLDALN
AEQQLYSTRRDLAKARYDYLMAWIKLHYYAGTLRDTDLARIDEAFVVAR
>Mature_448_residues
SRHPFKAALLTAILLGTAPLSQAMGPFQVYELALRNDPVYLGALKERDAGLENRVIGRAGLLPRVSYSYNKGRNDSKATL
KGSAQDTTDHRHYNSFGSSLTVQQPLIDYEAYANYRKGLAQALFADETFRSKSQELLVRVLTLYTQALFAQDQINIARAK
QQAFERQFAQNRQMFERGEGTRTDILEAESRNELAIAEQIEASDEQDAALRELAALLGEPSIDVAQLEPLRDSFQALVLA
PSSFTEWHALAMANSPILASQRQSLEVARYEVERNRAGHLPTVNAYASIRQNESDSGNTYNQRYDTNTIGIELSLPLYAG
GGTSASVRQANSKREQAEYELEGKTRETLIELRRQFNACASGVNKLRAYQKALISAQALVESTRQSILGGERISLDALNA
EQQLYSTRRDLAKARYDYLMAWIKLHYYAGTLRDTDLARIDEAFVVAR

Specific function: Involved in the secretion of alkaline protease [H]

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=439, Percent_Identity=26.1958997722096, Blast_Score=139, Evalue=4e-34,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010130 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 50204; Mature: 50073

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRHPFKAALLTAILLGTAPLSQAMGPFQVYELALRNDPVYLGALKERDAGLENRVIGRA
CCCCCHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHCCCCEEEEEHHHHCCCCHHHHHCCC
GLLPRVSYSYNKGRNDSKATLKGSAQDTTDHRHYNSFGSSLTVQQPLIDYEAYANYRKGL
CCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHCCCEEECCCHHHHHHHHHHHHHH
AQALFADETFRSKSQELLVRVLTLYTQALFAQDQINIARAKQQAFERQFAQNRQMFERGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GTRTDILEAESRNELAIAEQIEASDEQDAALRELAALLGEPSIDVAQLEPLRDSFQALVL
CCHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEE
APSSFTEWHALAMANSPILASQRQSLEVARYEVERNRAGHLPTVNAYASIRQNESDSGNT
CCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCC
YNQRYDTNTIGIELSLPLYAGGGTSASVRQANSKREQAEYELEGKTRETLIELRRQFNAC
CCCCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ASGVNKLRAYQKALISAQALVESTRQSILGGERISLDALNAEQQLYSTRRDLAKARYDYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
MAWIKLHYYAGTLRDTDLARIDEAFVVAR
HHHHHHHHHHCCCCCCHHHHHCCHHEECC
>Mature Secondary Structure 
SRHPFKAALLTAILLGTAPLSQAMGPFQVYELALRNDPVYLGALKERDAGLENRVIGRA
CCCCHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHCCCCEEEEEHHHHCCCCHHHHHCCC
GLLPRVSYSYNKGRNDSKATLKGSAQDTTDHRHYNSFGSSLTVQQPLIDYEAYANYRKGL
CCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHCCCEEECCCHHHHHHHHHHHHHH
AQALFADETFRSKSQELLVRVLTLYTQALFAQDQINIARAKQQAFERQFAQNRQMFERGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GTRTDILEAESRNELAIAEQIEASDEQDAALRELAALLGEPSIDVAQLEPLRDSFQALVL
CCHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEE
APSSFTEWHALAMANSPILASQRQSLEVARYEVERNRAGHLPTVNAYASIRQNESDSGNT
CCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCC
YNQRYDTNTIGIELSLPLYAGGGTSASVRQANSKREQAEYELEGKTRETLIELRRQFNAC
CCCCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ASGVNKLRAYQKALISAQALVESTRQSILGGERISLDALNAEQQLYSTRRDLAKARYDYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
MAWIKLHYYAGTLRDTDLARIDEAFVVAR
HHHHHHHHHHCCCCCCHHHHHCCHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1427098; 10984043 [H]