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The map label for this gene is pefL [H]

Identifier: 66046373

GI number: 66046373

Start: 3770226

End: 3771320

Strand: Reverse

Name: pefL [H]

Synonym: Psyr_3144

Alternate gene names: 66046373

Gene position: 3771320-3770226 (Counterclockwise)

Preceding gene: 66046374

Following gene: 66046372

Centisome position: 61.89

GC content: 65.11

Gene sequence:

>1095_bases
ATGAATTCATCAGTTTCTGCACGTCTGGCGCCTGTTTACGCGTTACGCGAACAGGTCGCCCGGCAGTGGCGTGGCAGTCC
GGCGCAACACGCCTGGCAATGGTGGGTGGCGGAGCTTCGCGCCTGCCTGCCGCCGCGTGTGCGTCGATGGCTGGGCCGCG
AGACCGTGGAGCAGGCGCTTGTCTGGCCATTGGTCGAGCCGCTGCCGGACGCATCGCCCCAGGTGCGGCGGGTATTGCTG
TTGCCGCCGTCGGCTGTGCTGTTGCAGACCCTGCAGCTTCCTGCGGCTGCGGCACGCAATCTGTCGACCGTGGTCGGCTA
CGAGCTGGATCGTTTCACACCATTTGACGCTGGCCAGTTGTACTTCGTCGCCCGTCAGGACAGTCGCAGCGCCAGCTTTG
TGCAGGTCACGCTGGTGGCAATCCTGCGCGAGCGGCTGGACGCGATACTCAGTGAGTGCGCCGAGCGGGGCTTGCGTCCC
GATGCCGTCGACGTCGGCACGGATGTGGGTGCTGAGCACCTGACTGCAGGGCAGCGCATGGGGATTGATCTGCTGCCGAT
GCCACTGCGCCCGCAACAGTCGCGCTCCGGCCATCGCTTGCAGCGCGGGCTGATCTGGCTGTGTGCCGGCCTGCTGCTGA
GCGCCATGTTGCTGTGGCTGAACGACCGACAGAGCCTGCTCGAGGACATGCAGGCCAGCGTCAAGGAGCAGAAGGCTCAG
GTTGGCGAGGTCCAACAACTGCGCCAGCAACTGACCAACACCCTGGGCGCCGCCAACTACCTGTTGCGCCGCAAGGCGGC
GCAGCCGCCGCTCTCGGCGCTGCTCAGCGAGCTGACCGCCTGCCTGCCTTCTGACACCTGGATCGAGCATATGGAAATCA
GCGACAGTGCCGAGGTGGCCTTTTCCGGCCAGAGCGCCAAGGCCAGTGCGCTGATCGGCCGCGTCAAGGACTGCCGAAGT
CTGGATAATGCGCAGTTTCAGGGGGTGATCCAGCCCGACACCAAAACCGGCAAGGATCAGTATTCCCTGCGCGCGCATTT
GCACCAGAACCAGCAGCAGGAGAACGCCGATGCGCCGTCCACTGACAAGCCGTGA

Upstream 100 bases:

>100_bases
CAGAACGACCGGGCGGCGTGACCGCCCTGTTACAGACCACTGTTTTATTGAGCCCATCGGAGGGAGGCGCACAACCCTAC
AGGGTGTTGCGTTGGCAAGA

Downstream 100 bases:

>100_bases
GCGGCGGGGCGCTGCCCTGATGATTCTGGCCCTTGTGCTGTGCCTGGCTTACTGGTTGTTGATCGACAGCTGGTTCGCCG
GGCCGCTGCGCGACATCAAC

Product: fimbrial assembly

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MNSSVSARLAPVYALREQVARQWRGSPAQHAWQWWVAELRACLPPRVRRWLGRETVEQALVWPLVEPLPDASPQVRRVLL
LPPSAVLLQTLQLPAAAARNLSTVVGYELDRFTPFDAGQLYFVARQDSRSASFVQVTLVAILRERLDAILSECAERGLRP
DAVDVGTDVGAEHLTAGQRMGIDLLPMPLRPQQSRSGHRLQRGLIWLCAGLLLSAMLLWLNDRQSLLEDMQASVKEQKAQ
VGEVQQLRQQLTNTLGAANYLLRRKAAQPPLSALLSELTACLPSDTWIEHMEISDSAEVAFSGQSAKASALIGRVKDCRS
LDNAQFQGVIQPDTKTGKDQYSLRAHLHQNQQQENADAPSTDKP

Sequences:

>Translated_364_residues
MNSSVSARLAPVYALREQVARQWRGSPAQHAWQWWVAELRACLPPRVRRWLGRETVEQALVWPLVEPLPDASPQVRRVLL
LPPSAVLLQTLQLPAAAARNLSTVVGYELDRFTPFDAGQLYFVARQDSRSASFVQVTLVAILRERLDAILSECAERGLRP
DAVDVGTDVGAEHLTAGQRMGIDLLPMPLRPQQSRSGHRLQRGLIWLCAGLLLSAMLLWLNDRQSLLEDMQASVKEQKAQ
VGEVQQLRQQLTNTLGAANYLLRRKAAQPPLSALLSELTACLPSDTWIEHMEISDSAEVAFSGQSAKASALIGRVKDCRS
LDNAQFQGVIQPDTKTGKDQYSLRAHLHQNQQQENADAPSTDKP
>Mature_364_residues
MNSSVSARLAPVYALREQVARQWRGSPAQHAWQWWVAELRACLPPRVRRWLGRETVEQALVWPLVEPLPDASPQVRRVLL
LPPSAVLLQTLQLPAAAARNLSTVVGYELDRFTPFDAGQLYFVARQDSRSASFVQVTLVAILRERLDAILSECAERGLRP
DAVDVGTDVGAEHLTAGQRMGIDLLPMPLRPQQSRSGHRLQRGLIWLCAGLLLSAMLLWLNDRQSLLEDMQASVKEQKAQ
VGEVQQLRQQLTNTLGAANYLLRRKAAQPPLSALLSELTACLPSDTWIEHMEISDSAEVAFSGQSAKASALIGRVKDCRS
LDNAQFQGVIQPDTKTGKDQYSLRAHLHQNQQQENADAPSTDKP

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins [H]

COG id: COG3166

COG function: function code NU; Tfp pilus assembly protein PilN

Gene ontology:

Cell location: Cell inner membrane (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP L family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007813 [H]

Pfam domain/function: PF05137 PilN [H]

EC number: NA

Molecular weight: Translated: 40392; Mature: 40392

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSVSARLAPVYALREQVARQWRGSPAQHAWQWWVAELRACLPPRVRRWLGRETVEQAL
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHCHHHHHHHH
VWPLVEPLPDASPQVRRVLLLPPSAVLLQTLQLPAAAARNLSTVVGYELDRFTPFDAGQL
HHHHHHCCCCCCHHHCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCE
YFVARQDSRSASFVQVTLVAILRERLDAILSECAERGLRPDAVDVGTDVGAEHLTAGQRM
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCHHHHHCCHHC
GIDLLPMPLRPQQSRSGHRLQRGLIWLCAGLLLSAMLLWLNDRQSLLEDMQASVKEQKAQ
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VGEVQQLRQQLTNTLGAANYLLRRKAAQPPLSALLSELTACLPSDTWIEHMEISDSAEVA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCEE
FSGQSAKASALIGRVKDCRSLDNAQFQGVIQPDTKTGKDQYSLRAHLHQNQQQENADAPS
ECCCCHHHHHHHHHHHHHHCCCCHHCCCEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
TDKP
CCCC
>Mature Secondary Structure
MNSSVSARLAPVYALREQVARQWRGSPAQHAWQWWVAELRACLPPRVRRWLGRETVEQAL
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHCHHHHHHHH
VWPLVEPLPDASPQVRRVLLLPPSAVLLQTLQLPAAAARNLSTVVGYELDRFTPFDAGQL
HHHHHHCCCCCCHHHCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCE
YFVARQDSRSASFVQVTLVAILRERLDAILSECAERGLRPDAVDVGTDVGAEHLTAGQRM
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCHHHHHCCHHC
GIDLLPMPLRPQQSRSGHRLQRGLIWLCAGLLLSAMLLWLNDRQSLLEDMQASVKEQKAQ
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VGEVQQLRQQLTNTLGAANYLLRRKAAQPPLSALLSELTACLPSDTWIEHMEISDSAEVA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCEE
FSGQSAKASALIGRVKDCRSLDNAQFQGVIQPDTKTGKDQYSLRAHLHQNQQQENADAPS
ECCCCHHHHHHHHHHHHHHCCCCHHCCCEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
TDKP
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]