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The map label for this gene is pncA [H]

Identifier: 66046354

GI number: 66046354

Start: 3747807

End: 3748451

Strand: Direct

Name: pncA [H]

Synonym: Psyr_3125

Alternate gene names: 66046354

Gene position: 3747807-3748451 (Clockwise)

Preceding gene: 66046352

Following gene: 66046355

Centisome position: 61.5

GC content: 62.79

Gene sequence:

>645_bases
ATGCCCCTTTCCCACAAGCCTGCCGATCCTCGTTGCGCCTTGCTGGTGATCGACATGCAGTACGACTTCATGCCCGGCGG
GCAACTGGCGGTGGCTGACGGCGATGCGCTGCTGCCGTTGATCAATCGCCTGGGTGCGCGCTTTTCCCAAGTGGTCATCA
CGCAGGACTGGCACCCTGCCGGGCACATTTCCTTTGCTTCCAGCCATGCGCAGCGCCTGCCGTTCGAGAGCATCACCCTG
CCCTACGGTCCGCAGACGCTGTGGCCGGATCATTGCGTACAAGGCAGTCACGGTGCGCAGTTGCATGCCGACCTCGACCT
GCCTCACGCGCAACTGGTCCTGCGCAAGGGCTGCAATGCGCATATCGACAGTTATTCGGCATTTCTGGAGGCAGACCGTA
CAACCCGCACAGGGCTGGCCGGGTACCTGACCGAGCGCGGCATCGATACGCTGTTCGTGGTCGGGCTGGCGCTGGACTTC
TGTGTCGCCTGGTCGGCGCAGGATGCACGCAGTGCCGGTTTCAACACTTTTGTGGTGGAAGACGCCTGTCGCGCCATCGA
CATGAACGGCTCGCTCGAGCACGCCTGGAAGACCATGCTGGGCATGGGTATCGAACGCGTTCAGAGCGCCGATCTGCTGG
GCTAG

Upstream 100 bases:

>100_bases
ATTCCCAAAAAATACTCTTGCCAAGGCCAGGTATGCCGCTTAGCCAACGTGGCGATAAACAGGCAAACTGAGCTCATTAC
TCCTTACCAGAGAACCCACG

Downstream 100 bases:

>100_bases
ACCGACACATTCTGCCAACTGGCTCAAACAATTCTCGATACGCCCCGCCGCTGGCGAACGACAGCGCGCGGCGCCTGTCT
GAACCATCGGCCCGACATTG

Product: nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MPLSHKPADPRCALLVIDMQYDFMPGGQLAVADGDALLPLINRLGARFSQVVITQDWHPAGHISFASSHAQRLPFESITL
PYGPQTLWPDHCVQGSHGAQLHADLDLPHAQLVLRKGCNAHIDSYSAFLEADRTTRTGLAGYLTERGIDTLFVVGLALDF
CVAWSAQDARSAGFNTFVVEDACRAIDMNGSLEHAWKTMLGMGIERVQSADLLG

Sequences:

>Translated_214_residues
MPLSHKPADPRCALLVIDMQYDFMPGGQLAVADGDALLPLINRLGARFSQVVITQDWHPAGHISFASSHAQRLPFESITL
PYGPQTLWPDHCVQGSHGAQLHADLDLPHAQLVLRKGCNAHIDSYSAFLEADRTTRTGLAGYLTERGIDTLFVVGLALDF
CVAWSAQDARSAGFNTFVVEDACRAIDMNGSLEHAWKTMLGMGIERVQSADLLG
>Mature_213_residues
PLSHKPADPRCALLVIDMQYDFMPGGQLAVADGDALLPLINRLGARFSQVVITQDWHPAGHISFASSHAQRLPFESITLP
YGPQTLWPDHCVQGSHGAQLHADLDLPHAQLVLRKGCNAHIDSYSAFLEADRTTRTGLAGYLTERGIDTLFVVGLALDFC
VAWSAQDARSAGFNTFVVEDACRAIDMNGSLEHAWKTMLGMGIERVQSADLLG

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=207, Percent_Identity=42.512077294686, Blast_Score=152, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI193206735, Length=207, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17543130, Length=207, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI71999339, Length=200, Percent_Identity=33, Blast_Score=97, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6321401, Length=194, Percent_Identity=34.020618556701, Blast_Score=106, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24648247, Length=210, Percent_Identity=35.7142857142857, Blast_Score=122, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 23280; Mature: 23149

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLSHKPADPRCALLVIDMQYDFMPGGQLAVADGDALLPLINRLGARFSQVVITQDWHPA
CCCCCCCCCCCEEEEEEEEEECCCCCCEEEEECCCHHHHHHHHHCHHHEEEEEECCCCCC
GHISFASSHAQRLPFESITLPYGPQTLWPDHCVQGSHGAQLHADLDLPHAQLVLRKGCNA
CCEEEHHHHHHHCCCCCEECCCCCCCCCHHHHCCCCCCCEEEECCCCCHHHHHHHCCCCC
HIDSYSAFLEADRTTRTGLAGYLTERGIDTLFVVGLALDFCVAWSAQDARSAGFNTFVVE
CHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEEH
DACRAIDMNGSLEHAWKTMLGMGIERVQSADLLG
HHHHEECCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PLSHKPADPRCALLVIDMQYDFMPGGQLAVADGDALLPLINRLGARFSQVVITQDWHPA
CCCCCCCCCCEEEEEEEEEECCCCCCEEEEECCCHHHHHHHHHCHHHEEEEEECCCCCC
GHISFASSHAQRLPFESITLPYGPQTLWPDHCVQGSHGAQLHADLDLPHAQLVLRKGCNA
CCEEEHHHHHHHCCCCCEECCCCCCCCCHHHHCCCCCCCEEEECCCCCHHHHHHHCCCCC
HIDSYSAFLEADRTTRTGLAGYLTERGIDTLFVVGLALDFCVAWSAQDARSAGFNTFVVE
CHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEEH
DACRAIDMNGSLEHAWKTMLGMGIERVQSADLLG
HHHHEECCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]