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The map label for this gene is gudD [H]

Identifier: 66046349

GI number: 66046349

Start: 3740656

End: 3742008

Strand: Direct

Name: gudD [H]

Synonym: Psyr_3120

Alternate gene names: 66046349

Gene position: 3740656-3742008 (Clockwise)

Preceding gene: 66046348

Following gene: 66046350

Centisome position: 61.39

GC content: 61.49

Gene sequence:

>1353_bases
ATGAACATTCAGCAATCCAATATCACCAGCAAGGCCCCCGTCATTACCGAGATGCAGGTTGTGCCCGTAGCGGGCCATGA
CGACATGCTGCTCAACCTGAGCGGTGCACATGGCCCCTACTTCACCCGCAACGTAGTCATTCTCAAGGACAACGCAGGGC
ATGTCGGTGTGGGCGAAGTGCCTGGCGGCGAGCTGATTCGCCAGACGCTGGAAGACGCGCGATCGCTGGTCGTCGGCAGC
AGCATTGGCACTTACCAGAAAATCCTCAACCAGGCACGCAAGGCGTTTGCCGATCGGGACTCCGGTGGCCGTGGCTTGCA
GACGTTCGACCTGCGCATTGCCATTCATGCGGTCACCGCGCTGGAAGCGGCGCTCCTCGACCTGCTCGGCCAGCATCTGG
AAGTACCGGTTGCGGCATTGCTGGGCGAAGGTCAGCAACGCGATCAGGTTGAAATGCTCGGTTACCTGTTTTATGTCGGC
GACCAGCGCAAGACCGATCTGGCCTACCGCAGCGAACCCGAGGCCGACAACGACTGGTTCCGTATCCGCCATGAAGAAGC
ACTGACGCCCGAGGCCGTGGTGCGCCTGGCCGAAGCCGCGCATCAGCGTTACGGCTTTCAGGACTTCAAGCTCAAGGGCG
GCGTGTTGAGCGGCGACCAGGAGATCGAAGCGGTGACGGCGCTCGCCGAACGCTTCCCCGATGCACGCATCACGCTGGAC
CCGAACGGTGCCTGGTCGCTCAAGGAAGCAATCCGTCTGTGCCGCGATCAGCATCATGTGCTGGCCTACGCCGAAGACCC
GTGCGGCGCGGAAAACGGTTATTCCGGTCGTGAAGTGATGGCCGAGTTCCGCCGCGCTACCGGCCTCAAGACGGCCACCA
ACATGATAGCCACCGACTGGCGGGAAATGGGTCACGCCATTCAGTTGCAGTCGGTGGACATTCCACTGGCCGACCCGCAT
TTCTGGACCATGCAGGGCTCGGTACGGGTGGCGCAGATGTGCAACGAGTGGGGCCTGACCTGGGGCTCGCACTCCAACAA
CCATTTTGATATTTCCCTGGCGATGTTCACCCACGTGGCCGCTGCAGCGCCGGGCACTATCACGGCGATCGATACCCACT
GGATCTGGCAGGACGGACAGCGTCTGACCAAGGCGCCGCTGCAAATCGTCGGTGGCAAGGTCGAGGTCCCGAACAAACCG
GGGCTCGGTGTCGAGCTGGACATGGACCAGCTGGCCAAGGCTCATGAACTGTACAAAGGCATGGGCCTGGGCGCTCGCAA
TGACGCAGTGGCCATGCAGTTTCTGATCCCGGACTGGACATTCAACAACAAGCAGCCTTGCCTGGTGCGTTGA

Upstream 100 bases:

>100_bases
CCGGTCGCCGCCATTCAGACCCCGGCGACCCACTGATGCACGTGCGCAGCCGCTTTTTCATCGGTCATATTTTCAAGGCC
TTACATTTAAAGGACCCGTC

Downstream 100 bases:

>100_bases
ATCGGTCCGACATTCGCCGTTGACTCGCCGCCTGTTCAACCTCTGGCTGGCACACGGCGCGCAACCCCCTTGCGCGCCGT
TTTTTTTAATCGAATTAGGC

Product: glucarate dehydratase

Products: NA

Alternate protein names: GDH; GlucD [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MNIQQSNITSKAPVITEMQVVPVAGHDDMLLNLSGAHGPYFTRNVVILKDNAGHVGVGEVPGGELIRQTLEDARSLVVGS
SIGTYQKILNQARKAFADRDSGGRGLQTFDLRIAIHAVTALEAALLDLLGQHLEVPVAALLGEGQQRDQVEMLGYLFYVG
DQRKTDLAYRSEPEADNDWFRIRHEEALTPEAVVRLAEAAHQRYGFQDFKLKGGVLSGDQEIEAVTALAERFPDARITLD
PNGAWSLKEAIRLCRDQHHVLAYAEDPCGAENGYSGREVMAEFRRATGLKTATNMIATDWREMGHAIQLQSVDIPLADPH
FWTMQGSVRVAQMCNEWGLTWGSHSNNHFDISLAMFTHVAAAAPGTITAIDTHWIWQDGQRLTKAPLQIVGGKVEVPNKP
GLGVELDMDQLAKAHELYKGMGLGARNDAVAMQFLIPDWTFNNKQPCLVR

Sequences:

>Translated_450_residues
MNIQQSNITSKAPVITEMQVVPVAGHDDMLLNLSGAHGPYFTRNVVILKDNAGHVGVGEVPGGELIRQTLEDARSLVVGS
SIGTYQKILNQARKAFADRDSGGRGLQTFDLRIAIHAVTALEAALLDLLGQHLEVPVAALLGEGQQRDQVEMLGYLFYVG
DQRKTDLAYRSEPEADNDWFRIRHEEALTPEAVVRLAEAAHQRYGFQDFKLKGGVLSGDQEIEAVTALAERFPDARITLD
PNGAWSLKEAIRLCRDQHHVLAYAEDPCGAENGYSGREVMAEFRRATGLKTATNMIATDWREMGHAIQLQSVDIPLADPH
FWTMQGSVRVAQMCNEWGLTWGSHSNNHFDISLAMFTHVAAAAPGTITAIDTHWIWQDGQRLTKAPLQIVGGKVEVPNKP
GLGVELDMDQLAKAHELYKGMGLGARNDAVAMQFLIPDWTFNNKQPCLVR
>Mature_450_residues
MNIQQSNITSKAPVITEMQVVPVAGHDDMLLNLSGAHGPYFTRNVVILKDNAGHVGVGEVPGGELIRQTLEDARSLVVGS
SIGTYQKILNQARKAFADRDSGGRGLQTFDLRIAIHAVTALEAALLDLLGQHLEVPVAALLGEGQQRDQVEMLGYLFYVG
DQRKTDLAYRSEPEADNDWFRIRHEEALTPEAVVRLAEAAHQRYGFQDFKLKGGVLSGDQEIEAVTALAERFPDARITLD
PNGAWSLKEAIRLCRDQHHVLAYAEDPCGAENGYSGREVMAEFRRATGLKTATNMIATDWREMGHAIQLQSVDIPLADPH
FWTMQGSVRVAQMCNEWGLTWGSHSNNHFDISLAMFTHVAAAAPGTITAIDTHWIWQDGQRLTKAPLQIVGGKVEVPNKP
GLGVELDMDQLAKAHELYKGMGLGARNDAVAMQFLIPDWTFNNKQPCLVR

Specific function: Catalyzes the dehydration of glucarate to 5-keto-4- deoxy-D-glucarate (5-kdGluc) [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GlucD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789150, Length=439, Percent_Identity=74.2596810933941, Blast_Score=707, Evalue=0.0,
Organism=Escherichia coli, GI1789151, Length=445, Percent_Identity=64.7191011235955, Blast_Score=581, Evalue=1e-167,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017653
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.40 [H]

Molecular weight: Translated: 49558; Mature: 49558

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIQQSNITSKAPVITEMQVVPVAGHDDMLLNLSGAHGPYFTRNVVILKDNAGHVGVGEV
CCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEECCCCCCCEEEEEEEEECCCCCCCCCCC
PGGELIRQTLEDARSLVVGSSIGTYQKILNQARKAFADRDSGGRGLQTFDLRIAIHAVTA
CCHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEHHHHHH
LEAALLDLLGQHLEVPVAALLGEGQQRDQVEMLGYLFYVGDQRKTDLAYRSEPEADNDWF
HHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCEE
RIRHEEALTPEAVVRLAEAAHQRYGFQDFKLKGGVLSGDQEIEAVTALAERFPDARITLD
EEECHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEC
PNGAWSLKEAIRLCRDQHHVLAYAEDPCGAENGYSGREVMAEFRRATGLKTATNMIATDW
CCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
REMGHAIQLQSVDIPLADPHFWTMQGSVRVAQMCNEWGLTWGSHSNNHFDISLAMFTHVA
HHCCCEEEEEECCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHH
AAAPGTITAIDTHWIWQDGQRLTKAPLQIVGGKVEVPNKPGLGVELDMDQLAKAHELYKG
HCCCCEEEEEEEEEEECCCCHHHHCCHHHCCCEEECCCCCCCCEEECHHHHHHHHHHHHH
MGLGARNDAVAMQFLIPDWTFNNKQPCLVR
CCCCCCCCCEEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MNIQQSNITSKAPVITEMQVVPVAGHDDMLLNLSGAHGPYFTRNVVILKDNAGHVGVGEV
CCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEECCCCCCCEEEEEEEEECCCCCCCCCCC
PGGELIRQTLEDARSLVVGSSIGTYQKILNQARKAFADRDSGGRGLQTFDLRIAIHAVTA
CCHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEHHHHHH
LEAALLDLLGQHLEVPVAALLGEGQQRDQVEMLGYLFYVGDQRKTDLAYRSEPEADNDWF
HHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCEE
RIRHEEALTPEAVVRLAEAAHQRYGFQDFKLKGGVLSGDQEIEAVTALAERFPDARITLD
EEECHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEC
PNGAWSLKEAIRLCRDQHHVLAYAEDPCGAENGYSGREVMAEFRRATGLKTATNMIATDW
CCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
REMGHAIQLQSVDIPLADPHFWTMQGSVRVAQMCNEWGLTWGSHSNNHFDISLAMFTHVA
HHCCCEEEEEECCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHH
AAAPGTITAIDTHWIWQDGQRLTKAPLQIVGGKVEVPNKPGLGVELDMDQLAKAHELYKG
HCCCCEEEEEEEEEEECCCCHHHHCCHHHCCCEEECCCCCCCCEEECHHHHHHHHHHHHH
MGLGARNDAVAMQFLIPDWTFNNKQPCLVR
CCCCCCCCCEEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9772161 [H]