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The map label for this gene is tolC [H]

Identifier: 66046308

GI number: 66046308

Start: 3680341

End: 3681816

Strand: Reverse

Name: tolC [H]

Synonym: Psyr_3077

Alternate gene names: 66046308

Gene position: 3681816-3680341 (Counterclockwise)

Preceding gene: 66046309

Following gene: 66046307

Centisome position: 60.42

GC content: 56.78

Gene sequence:

>1476_bases
ATGCTACCGCAAGGACGCGCCGTGTATCCTCATGCCCTCAAACTGGCTTTGCTGTTCCTGCTCACCATCGCGTTGGCTCC
CTCGTTTGCAGCGCCTGCCACCGTCAATTTGCCAGGCGATCAGGGCGTTGTTCATGACCGCCGGGATCTGCTAAAGCGCC
ACGCGGATCTTTTAGATGTTTATAAAGCCGCCTTTTTCAACGATGCGCGATTGTCCGCAGCGCGTCATGCTTACAGGGCA
CAACGCGAAGGCGTGCCTCAGGCGCGTGCAGGCCTGCTGCCGAATCTGACCGCCGGGGCAACGTCAGAAGTAACCCGTCT
GGAGCGAGACGAGCCTTCCCTAAGCCGAGAGCGAAGTGGCACAACGTTTCGTGCCAACCTCAGTCAGCCGTTGTTCCGCA
TTGATCGCTGGTTTCAATTGAAGGCTGCGCAATCGAGCCTGGATCAAGCCGGGCTTGAGCTGTCGGCCAAAGAGCAAAAC
TTGCTGCTGACCGCTGCCCAGGCCTACTTTGAAACACTGCGCCAACTGGACGCGTTCGCAGCCGCCCAGGCTGAAGAAGC
TGCGCTATTGCGTCAGCGCGACCAGGCTCAAGGGCGTCTGGAGGATGGTGCTTCAAGCATCACGGACGTGTATGACGCCC
AGGCGGCCTATGACAATGCCCGGGCCAACCGGCAACTGGTGCAGCGAAAAGTAGATGATGCCTATGAAGCGCTGGAGCGC
CTGACCAAGCACAGCTACACGTCAATTGCAGGCATAAGACACCAGATGCCGGTAGAGGCGCCCATACCCAATGATGCCCG
TGCCTGGGTCGATATTGCCCTGCGGCAAAATCTTGAGCTGCAAGCCAGCGAGCGTGCAGTCAGTGCTGCCGAGCAAACGC
TTAATCAGCGTAAAGCCGGGCATGCCCCCACTGTTGACGCTGTTGCTTCTTATCGCAAAGGTGATAACGACAGCTTTGGC
TACAGTAACCCGACTGACTTCGGCAGTAACGGTTATCGCGACCAGGTCGCGCAAAGCAGCATTGGCATTGAGCTCAATAT
CCCTCTTTACTCGGGCGGAATGACCAACTCCCAGATCAAGGAGTCCACCGAGCGTCTGTATCAGAGCCAGGACGAACAGG
AAGACCGCCGCCGCGATGTGGTGCTCAATACACGCAATGCGTTTCGCGGCATCAATACGGGCATAGAGCAGATTGCAGCC
CGACGCCAAAGCATCATTTCCGGTCAGAAATCCGTGGAAGCCAATCAGGTGGGCGTGGACGTTGGCTCTCGCAATATTGC
CGATGTACTCAATGCTCAGCGTCAGTTGTACGCTGCCGTGCGCGATTATAACGATGCCCGGTATGACTACATTCTCGATA
ACCTCAAGCTCAAACAGTCTGCTGGCATCCTCAGTCCTGATGACCTGAAAGCACTTGCGTTATATCTCAAGCAAGACTAC
GACCCTGCTCGCGATTTTCTGCCGCCAGGAGTCTGA

Upstream 100 bases:

>100_bases
TAAACTCAAAAAAAGTTCAACAAATCGGATACAAGGTCGATACAAATAATTGACGCCGCCATAATGGCGTTCTAGTATTG
CCCACCTTTTTGATATCAAT

Downstream 100 bases:

>100_bases
TATTCGTCACATCGGTAGTTAACGCAGTAGCGAACTGAACCCGTCGGTTATGCAAATCGCATATCCAATAAAGTGAATGC
TTACAGCTAATTAGTAAATT

Product: Type I secretion outer membrane protein, TolC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MLPQGRAVYPHALKLALLFLLTIALAPSFAAPATVNLPGDQGVVHDRRDLLKRHADLLDVYKAAFFNDARLSAARHAYRA
QREGVPQARAGLLPNLTAGATSEVTRLERDEPSLSRERSGTTFRANLSQPLFRIDRWFQLKAAQSSLDQAGLELSAKEQN
LLLTAAQAYFETLRQLDAFAAAQAEEAALLRQRDQAQGRLEDGASSITDVYDAQAAYDNARANRQLVQRKVDDAYEALER
LTKHSYTSIAGIRHQMPVEAPIPNDARAWVDIALRQNLELQASERAVSAAEQTLNQRKAGHAPTVDAVASYRKGDNDSFG
YSNPTDFGSNGYRDQVAQSSIGIELNIPLYSGGMTNSQIKESTERLYQSQDEQEDRRRDVVLNTRNAFRGINTGIEQIAA
RRQSIISGQKSVEANQVGVDVGSRNIADVLNAQRQLYAAVRDYNDARYDYILDNLKLKQSAGILSPDDLKALALYLKQDY
DPARDFLPPGV

Sequences:

>Translated_491_residues
MLPQGRAVYPHALKLALLFLLTIALAPSFAAPATVNLPGDQGVVHDRRDLLKRHADLLDVYKAAFFNDARLSAARHAYRA
QREGVPQARAGLLPNLTAGATSEVTRLERDEPSLSRERSGTTFRANLSQPLFRIDRWFQLKAAQSSLDQAGLELSAKEQN
LLLTAAQAYFETLRQLDAFAAAQAEEAALLRQRDQAQGRLEDGASSITDVYDAQAAYDNARANRQLVQRKVDDAYEALER
LTKHSYTSIAGIRHQMPVEAPIPNDARAWVDIALRQNLELQASERAVSAAEQTLNQRKAGHAPTVDAVASYRKGDNDSFG
YSNPTDFGSNGYRDQVAQSSIGIELNIPLYSGGMTNSQIKESTERLYQSQDEQEDRRRDVVLNTRNAFRGINTGIEQIAA
RRQSIISGQKSVEANQVGVDVGSRNIADVLNAQRQLYAAVRDYNDARYDYILDNLKLKQSAGILSPDDLKALALYLKQDY
DPARDFLPPGV
>Mature_491_residues
MLPQGRAVYPHALKLALLFLLTIALAPSFAAPATVNLPGDQGVVHDRRDLLKRHADLLDVYKAAFFNDARLSAARHAYRA
QREGVPQARAGLLPNLTAGATSEVTRLERDEPSLSRERSGTTFRANLSQPLFRIDRWFQLKAAQSSLDQAGLELSAKEQN
LLLTAAQAYFETLRQLDAFAAAQAEEAALLRQRDQAQGRLEDGASSITDVYDAQAAYDNARANRQLVQRKVDDAYEALER
LTKHSYTSIAGIRHQMPVEAPIPNDARAWVDIALRQNLELQASERAVSAAEQTLNQRKAGHAPTVDAVASYRKGDNDSFG
YSNPTDFGSNGYRDQVAQSSIGIELNIPLYSGGMTNSQIKESTERLYQSQDEQEDRRRDVVLNTRNAFRGINTGIEQIAA
RRQSIISGQKSVEANQVGVDVGSRNIADVLNAQRQLYAAVRDYNDARYDYILDNLKLKQSAGILSPDDLKALALYLKQDY
DPARDFLPPGV

Specific function: May be specialized for signal sequence independent, extracellular secretion in Gram-negative bacteria [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=421, Percent_Identity=31.353919239905, Blast_Score=200, Evalue=2e-52,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010130 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 54325; Mature: 54325

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPQGRAVYPHALKLALLFLLTIALAPSFAAPATVNLPGDQGVVHDRRDLLKRHADLLDV
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHH
YKAAFFNDARLSAARHAYRAQREGVPQARAGLLPNLTAGATSEVTRLERDEPSLSRERSG
HHHHHHCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHCCCCCHHHHCCC
TTFRANLSQPLFRIDRWFQLKAAQSSLDQAGLELSAKEQNLLLTAAQAYFETLRQLDAFA
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHHHHHHHH
AAQAEEAALLRQRDQAQGRLEDGASSITDVYDAQAAYDNARANRQLVQRKVDDAYEALER
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTKHSYTSIAGIRHQMPVEAPIPNDARAWVDIALRQNLELQASERAVSAAEQTLNQRKAG
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
HAPTVDAVASYRKGDNDSFGYSNPTDFGSNGYRDQVAQSSIGIELNIPLYSGGMTNSQIK
CCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCEEEECEEECCCCCHHHHH
ESTERLYQSQDEQEDRRRDVVLNTRNAFRGINTGIEQIAARRQSIISGQKSVEANQVGVD
HHHHHHHHCCCHHHHHHHCEEEECHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHCCC
VGSRNIADVLNAQRQLYAAVRDYNDARYDYILDNLKLKQSAGILSPDDLKALALYLKQDY
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHCC
DPARDFLPPGV
CHHHHCCCCCC
>Mature Secondary Structure
MLPQGRAVYPHALKLALLFLLTIALAPSFAAPATVNLPGDQGVVHDRRDLLKRHADLLDV
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHH
YKAAFFNDARLSAARHAYRAQREGVPQARAGLLPNLTAGATSEVTRLERDEPSLSRERSG
HHHHHHCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHCCCCCHHHHCCC
TTFRANLSQPLFRIDRWFQLKAAQSSLDQAGLELSAKEQNLLLTAAQAYFETLRQLDAFA
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHHHHHHHH
AAQAEEAALLRQRDQAQGRLEDGASSITDVYDAQAAYDNARANRQLVQRKVDDAYEALER
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTKHSYTSIAGIRHQMPVEAPIPNDARAWVDIALRQNLELQASERAVSAAEQTLNQRKAG
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
HAPTVDAVASYRKGDNDSFGYSNPTDFGSNGYRDQVAQSSIGIELNIPLYSGGMTNSQIK
CCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCEEEECEEECCCCCHHHHH
ESTERLYQSQDEQEDRRRDVVLNTRNAFRGINTGIEQIAARRQSIISGQKSVEANQVGVD
HHHHHHHHCCCHHHHHHHCEEEECHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHCCC
VGSRNIADVLNAQRQLYAAVRDYNDARYDYILDNLKLKQSAGILSPDDLKALALYLKQDY
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHCC
DPARDFLPPGV
CHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]