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The map label for this gene is dlpA [H]

Identifier: 66046302

GI number: 66046302

Start: 3670279

End: 3671469

Strand: Reverse

Name: dlpA [H]

Synonym: Psyr_3071

Alternate gene names: 66046302

Gene position: 3671469-3670279 (Counterclockwise)

Preceding gene: 66046303

Following gene: 66046301

Centisome position: 60.25

GC content: 60.03

Gene sequence:

>1191_bases
ATGACCACTCAAATGCTCCGAAAGCGCATATGTGTCTTGCCCGGTGACGGGATCGGTATTGAAGTCATGGAGGCGGCGCT
TCCGGTGATTGCGGCGCTGAACCTGCCGTTCGATCTGGTACCGGCGGACATAGGTTGGCAATGCTGGAGGGCCGAGGGGG
AAACCGTTCCGGCCCGGACCTGGGAGATCATGGCCAGTTGCGACGCGACATTGCTTGGCGCAATCACCAGCAAACCTCTG
GCAGAGGCCGAAGCAGAGTTGCCACTGACGCTTCAGGGCCAGAACCGCCGCTATGTCAGTCCGGTCATTCAGCTCAGGCA
GAATCTCGACCTGTACGCCAATGTACGCCCGGTCACTGACATGACTGGCGAAAACAGATTTCGCTTTGTCGTGATCAGGG
AAAACACCGAAGGGCTTTATGCGGGGCTGGACTTCGGTTGTATCCCGGATGCACTGGTGCCGCTGCTTGAAGAGCGGGAA
GCCCTCGGCGCGGCGTGGCAGCGAGAAGGGCAGGACGATGCGACGGTGACGTTGCGCCTGCAGACGCGAAGTGGCCTGAT
GCGTATTTTCGAATACGCCTTCGAGTACGCCCGCAAGAACCACTTCACGCGGGTCACGTTTGTCGACAAACCTATGGTCC
TCAGGCACAGCAGCGCGTTTGCACGGGGCATCTTCGAAGCAACAGCTCAGCGTTATCCGGACGTGAGCGGGGTCATCGAA
AACGTTGATGCGGTTGCCCTGTGGATGGTGCAGCGCCCCGAGCGCTTCGGGGTGATCGTTGCCGAAAACATGTTTGGCGA
TATTCTGTCCGATCTGGGCGCCGGAGTCATGGGTGGCCTGGGGTTCGCGCCCAGTGGCAACTTCGGCAATTCAGGGGCTT
ATTTCGAACCGGTACACGGCAGTGCGCCTGCCCATGCCGGATTGAACAAGGCCAACCCCGGCGCGATGTTCATGGCCATC
GCGCTGATGCTTGAGCATCTGGGGTTTCCGACCGAGTCGGCATGCATCAGCCAGGCCGTCAGTCTGGTTTCGCGCACATC
CACACGCACGTATGACCTTGGCGGTTCCCATACCACGGCCCAGGTGGGCGAAGCGATCGTTCAGCAGTGCATCGAGTTGC
AGTCCCGCACGTTTGGGTATCGCAACGAGCAGGCTTCGGCACCGGGAGAACGTCATGTCAGCGCACTTTGA

Upstream 100 bases:

>100_bases
AGGTCATGTATCAGCAGGACAAGGTGGCGCGCCTTGCGCCGCGAGCGGTGTTGTCGACCATCGCCACCGGCTTGCCGAAC
GGCGCGAAGGGAGTCGCGCT

Downstream 100 bases:

>100_bases
AGCGGTGCCCATTGCGCAAGCCGTGCTGGACGCATGTGAACATCTCGACACTGCGTCCTTGTCTGATGCGCTGGACAGCC
TGGGGATCGACGGCGGGCTT

Product: 3-isopropylmalate dehydrogenase

Products: @HYDR01.txt*Oxaloglycolate!; NADH. [C]

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MTTQMLRKRICVLPGDGIGIEVMEAALPVIAALNLPFDLVPADIGWQCWRAEGETVPARTWEIMASCDATLLGAITSKPL
AEAEAELPLTLQGQNRRYVSPVIQLRQNLDLYANVRPVTDMTGENRFRFVVIRENTEGLYAGLDFGCIPDALVPLLEERE
ALGAAWQREGQDDATVTLRLQTRSGLMRIFEYAFEYARKNHFTRVTFVDKPMVLRHSSAFARGIFEATAQRYPDVSGVIE
NVDAVALWMVQRPERFGVIVAENMFGDILSDLGAGVMGGLGFAPSGNFGNSGAYFEPVHGSAPAHAGLNKANPGAMFMAI
ALMLEHLGFPTESACISQAVSLVSRTSTRTYDLGGSHTTAQVGEAIVQQCIELQSRTFGYRNEQASAPGERHVSAL

Sequences:

>Translated_396_residues
MTTQMLRKRICVLPGDGIGIEVMEAALPVIAALNLPFDLVPADIGWQCWRAEGETVPARTWEIMASCDATLLGAITSKPL
AEAEAELPLTLQGQNRRYVSPVIQLRQNLDLYANVRPVTDMTGENRFRFVVIRENTEGLYAGLDFGCIPDALVPLLEERE
ALGAAWQREGQDDATVTLRLQTRSGLMRIFEYAFEYARKNHFTRVTFVDKPMVLRHSSAFARGIFEATAQRYPDVSGVIE
NVDAVALWMVQRPERFGVIVAENMFGDILSDLGAGVMGGLGFAPSGNFGNSGAYFEPVHGSAPAHAGLNKANPGAMFMAI
ALMLEHLGFPTESACISQAVSLVSRTSTRTYDLGGSHTTAQVGEAIVQQCIELQSRTFGYRNEQASAPGERHVSAL
>Mature_395_residues
TTQMLRKRICVLPGDGIGIEVMEAALPVIAALNLPFDLVPADIGWQCWRAEGETVPARTWEIMASCDATLLGAITSKPLA
EAEAELPLTLQGQNRRYVSPVIQLRQNLDLYANVRPVTDMTGENRFRFVVIRENTEGLYAGLDFGCIPDALVPLLEEREA
LGAAWQREGQDDATVTLRLQTRSGLMRIFEYAFEYARKNHFTRVTFVDKPMVLRHSSAFARGIFEATAQRYPDVSGVIEN
VDAVALWMVQRPERFGVIVAENMFGDILSDLGAGVMGGLGFAPSGNFGNSGAYFEPVHGSAPAHAGLNKANPGAMFMAIA
LMLEHLGFPTESACISQAVSLVSRTSTRTYDLGGSHTTAQVGEAIVQQCIELQSRTFGYRNEQASAPGERHVSAL

Specific function: Unknown

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To M.jannaschii MJ0644 in the C-terminal section [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=379, Percent_Identity=33.245382585752, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI28178816, Length=365, Percent_Identity=28.4931506849315, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI28178821, Length=365, Percent_Identity=28.2191780821918, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI4758582, Length=378, Percent_Identity=26.7195767195767, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI28178838, Length=341, Percent_Identity=27.5659824046921, Blast_Score=121, Evalue=9e-28,
Organism=Homo sapiens, GI28178819, Length=192, Percent_Identity=31.7708333333333, Blast_Score=99, Evalue=6e-21,
Organism=Escherichia coli, GI1788101, Length=386, Percent_Identity=32.1243523316062, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI87081683, Length=385, Percent_Identity=32.4675324675325, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1787381, Length=403, Percent_Identity=27.2952853598015, Blast_Score=119, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71986051, Length=372, Percent_Identity=32.7956989247312, Blast_Score=190, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17550882, Length=368, Percent_Identity=27.7173913043478, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17505779, Length=370, Percent_Identity=28.1081081081081, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI25144293, Length=381, Percent_Identity=27.5590551181102, Blast_Score=124, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6322097, Length=376, Percent_Identity=33.7765957446808, Blast_Score=166, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6324709, Length=370, Percent_Identity=30.8108108108108, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6324291, Length=377, Percent_Identity=29.1777188328912, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6319830, Length=392, Percent_Identity=29.3367346938776, Blast_Score=136, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24643270, Length=373, Percent_Identity=32.9758713136729, Blast_Score=178, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24643268, Length=373, Percent_Identity=32.9758713136729, Blast_Score=178, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24661184, Length=372, Percent_Identity=31.7204301075269, Blast_Score=168, Evalue=5e-42,
Organism=Drosophila melanogaster, GI161078637, Length=368, Percent_Identity=27.9891304347826, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI161078633, Length=368, Percent_Identity=27.9891304347826, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24650122, Length=368, Percent_Identity=27.9891304347826, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI161078635, Length=368, Percent_Identity=27.9891304347826, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI161078639, Length=363, Percent_Identity=28.099173553719, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI281362242, Length=372, Percent_Identity=28.2258064516129, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24648872, Length=372, Percent_Identity=28.2258064516129, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI20130355, Length=364, Percent_Identity=23.9010989010989, Blast_Score=103, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR005493 [H]

Pfam domain/function: PF00180 Iso_dh; PF03737 Methyltransf_6 [H]

EC number: 1.1.1.93 [C]

Molecular weight: Translated: 43185; Mature: 43054

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQMLRKRICVLPGDGIGIEVMEAALPVIAALNLPFDLVPADIGWQCWRAEGETVPART
CCHHHHHHCEEECCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCEEECCCCCCCCCHH
WEIMASCDATLLGAITSKPLAEAEAELPLTLQGQNRRYVSPVIQLRQNLDLYANVRPVTD
HHHHHHCCHHHHHHHHCCCHHHCCCCCCEEEECCCCCHHHHHHHHHHCCCEEECCCCCCC
MTGENRFRFVVIRENTEGLYAGLDFGCIPDALVPLLEEREALGAAWQREGQDDATVTLRL
CCCCCEEEEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
QTRSGLMRIFEYAFEYARKNHFTRVTFVDKPMVLRHSSAFARGIFEATAQRYPDVSGVIE
ECHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEECCHHHHHHHHHHHHHHCCCHHHHHH
NVDAVALWMVQRPERFGVIVAENMFGDILSDLGAGVMGGLGFAPSGNFGNSGAYFEPVHG
CCHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCEECCCCC
SAPAHAGLNKANPGAMFMAIALMLEHLGFPTESACISQAVSLVSRTSTRTYDLGGSHTTA
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCHHH
QVGEAIVQQCIELQSRTFGYRNEQASAPGERHVSAL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCC
>Mature Secondary Structure 
TTQMLRKRICVLPGDGIGIEVMEAALPVIAALNLPFDLVPADIGWQCWRAEGETVPART
CHHHHHHCEEECCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCEEECCCCCCCCCHH
WEIMASCDATLLGAITSKPLAEAEAELPLTLQGQNRRYVSPVIQLRQNLDLYANVRPVTD
HHHHHHCCHHHHHHHHCCCHHHCCCCCCEEEECCCCCHHHHHHHHHHCCCEEECCCCCCC
MTGENRFRFVVIRENTEGLYAGLDFGCIPDALVPLLEEREALGAAWQREGQDDATVTLRL
CCCCCEEEEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
QTRSGLMRIFEYAFEYARKNHFTRVTFVDKPMVLRHSSAFARGIFEATAQRYPDVSGVIE
ECHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEECCHHHHHHHHHHHHHHCCCHHHHHH
NVDAVALWMVQRPERFGVIVAENMFGDILSDLGAGVMGGLGFAPSGNFGNSGAYFEPVHG
CCHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCEECCCCC
SAPAHAGLNKANPGAMFMAIALMLEHLGFPTESACISQAVSLVSRTSTRTYDLGGSHTTA
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCHHH
QVGEAIVQQCIELQSRTFGYRNEQASAPGERHVSAL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.28 {NAD}} 2.3 {L-(+)-tartrate}} [C]

Substrates: Tartrate; NAD(+) [C]

Specific reaction: Tartrate + NAD(+) = @HYDR01.txt*Oxaloglycolate! + NADH. [C]

General reaction: Redox reaction [C]

Inhibitor: ATP; Dihydroxy fumarate; meso-Tartrate; Oxaloacetate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7891566 [H]