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The map label for this gene is yafC [C]

Identifier: 66046235

GI number: 66046235

Start: 3599856

End: 3600815

Strand: Reverse

Name: yafC [C]

Synonym: Psyr_3004

Alternate gene names: 66046235

Gene position: 3600815-3599856 (Counterclockwise)

Preceding gene: 66046236

Following gene: 66046234

Centisome position: 59.09

GC content: 62.08

Gene sequence:

>960_bases
GTGTTTGATCTCAACGATCTGTTTTATTTCGTCCAGGTGGTCGAGTGCCTCGGCTTTGCGCCGGCAAGCCGCAAGCTGGG
GATCCCCAAGTCCAAGCTCAGCCGGCGCATTGCCGGACTCGAGGAGCGCCTGGGTGTTCGGCTGATTCACCGCTCGACGC
GCACCTTTGCGGTCACCGAAAGCGGGCGTGACTATTACGCTCACTGCGTGGCCATGCTGGTCGAGGCCGAGGCGGCCGAA
GAGGCCATCCTGCGTTCCAAATCCGAACCGCAGGGCAGGGTGGTGATCAGTTGTCCCCCTGCTTTGGCGGCGATGGGCTT
CAACGAGACCATCGCCCGTTTCGCGGCCGAGCACCCCAAGGTTCAGATCCAGGTCGAAAGTACCAACCGACGCGTCGACC
CGCTGACCGAGAACATCGACATTGCGCTGCGTGTGCGCTTTCCACCGCTGGAGGACGACGGGCTGGTGGTCAAGAAGCTT
GGGCACAGTGCCCAGCGTCTGGTGGCACGCGCCGACCTGGCGGCCAGGCTGTCGGCAACGCCTTCTCTCGACGCGCTGTC
GACGCTGCCCAGCCTCGACATGCCTTCTTCGAATCATGAGCACCGCTGGTTGCTGGAGACGCACCCCGGCCACTATGCCG
AAATACGCCACACGCCCCGGCTCATCAGTTCGGACCTGGACATGATGCTGCAGGCCGCGTTGCAGGGTGTCGGTGTGGCC
CAGCTTCCCGAGCTGATTGTCAGGGAAGCCTTGCAGGACGGACGCCTGGTAGAGCTGTTCAGCGAGCACCGACCCAAGTG
CGGTATCGTGCATGCCGTGTTCGCCTCGCGCCGTGGCCTGCTTCCTGCCGTCAGAGGCCTGCTGGATGTGTTCGAGACCT
CATTCAAAGAGCTGAGCCTGAGCCCCGACTCACTCTATTGTTCTCTTAATAGAACGAACAGTCGCATTCAAGACCACTAA

Upstream 100 bases:

>100_bases
CATTTCTGACTGCCGAATGACCCGGCGCGATCCTCCGTCCCGGTCTTGAGGCACGGGGCACGGCGCTGCATACTCCCGGC
TCTGAAAACGAGGTGGCCGG

Downstream 100 bases:

>100_bases
TCATCTATTGCGATCAAGGATAGGATGCTCGCCACTTCATCGCTCAGTGGCGATTTTCTTATGGGAGCTACGTTATGAAT
CTATTGCACCTCGACTCCAG

Product: regulatory protein, LysR:LysR, substrate-binding

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MFDLNDLFYFVQVVECLGFAPASRKLGIPKSKLSRRIAGLEERLGVRLIHRSTRTFAVTESGRDYYAHCVAMLVEAEAAE
EAILRSKSEPQGRVVISCPPALAAMGFNETIARFAAEHPKVQIQVESTNRRVDPLTENIDIALRVRFPPLEDDGLVVKKL
GHSAQRLVARADLAARLSATPSLDALSTLPSLDMPSSNHEHRWLLETHPGHYAEIRHTPRLISSDLDMMLQAALQGVGVA
QLPELIVREALQDGRLVELFSEHRPKCGIVHAVFASRRGLLPAVRGLLDVFETSFKELSLSPDSLYCSLNRTNSRIQDH

Sequences:

>Translated_319_residues
MFDLNDLFYFVQVVECLGFAPASRKLGIPKSKLSRRIAGLEERLGVRLIHRSTRTFAVTESGRDYYAHCVAMLVEAEAAE
EAILRSKSEPQGRVVISCPPALAAMGFNETIARFAAEHPKVQIQVESTNRRVDPLTENIDIALRVRFPPLEDDGLVVKKL
GHSAQRLVARADLAARLSATPSLDALSTLPSLDMPSSNHEHRWLLETHPGHYAEIRHTPRLISSDLDMMLQAALQGVGVA
QLPELIVREALQDGRLVELFSEHRPKCGIVHAVFASRRGLLPAVRGLLDVFETSFKELSLSPDSLYCSLNRTNSRIQDH
>Mature_319_residues
MFDLNDLFYFVQVVECLGFAPASRKLGIPKSKLSRRIAGLEERLGVRLIHRSTRTFAVTESGRDYYAHCVAMLVEAEAAE
EAILRSKSEPQGRVVISCPPALAAMGFNETIARFAAEHPKVQIQVESTNRRVDPLTENIDIALRVRFPPLEDDGLVVKKL
GHSAQRLVARADLAARLSATPSLDALSTLPSLDMPSSNHEHRWLLETHPGHYAEIRHTPRLISSDLDMMLQAALQGVGVA
QLPELIVREALQDGRLVELFSEHRPKCGIVHAVFASRRGLLPAVRGLLDVFETSFKELSLSPDSLYCSLNRTNSRIQDH

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=292, Percent_Identity=26.7123287671233, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI87081978, Length=303, Percent_Identity=28.7128712871287, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI145693193, Length=272, Percent_Identity=26.4705882352941, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787589, Length=279, Percent_Identity=26.1648745519713, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789639, Length=310, Percent_Identity=24.1935483870968, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1789440, Length=268, Percent_Identity=25.7462686567164, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787128, Length=304, Percent_Identity=24.3421052631579, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1789173, Length=263, Percent_Identity=24.3346007604563, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1787879, Length=148, Percent_Identity=29.7297297297297, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35438; Mature: 35438

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDLNDLFYFVQVVECLGFAPASRKLGIPKSKLSRRIAGLEERLGVRLIHRSTRTFAVTE
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCEEEEEC
SGRDYYAHCVAMLVEAEAAEEAILRSKSEPQGRVVISCPPALAAMGFNETIARFAAEHPK
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHCCCHHHHHHHHHCCCE
VQIQVESTNRRVDPLTENIDIALRVRFPPLEDDGLVVKKLGHSAQRLVARADLAARLSAT
EEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHCCC
PSLDALSTLPSLDMPSSNHEHRWLLETHPGHYAEIRHTPRLISSDLDMMLQAALQGVGVA
CCHHHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCHH
QLPELIVREALQDGRLVELFSEHRPKCGIVHAVFASRRGLLPAVRGLLDVFETSFKELSL
HHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
SPDSLYCSLNRTNSRIQDH
CCCCEEEEECCCCCCCCCC
>Mature Secondary Structure
MFDLNDLFYFVQVVECLGFAPASRKLGIPKSKLSRRIAGLEERLGVRLIHRSTRTFAVTE
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCEEEEEC
SGRDYYAHCVAMLVEAEAAEEAILRSKSEPQGRVVISCPPALAAMGFNETIARFAAEHPK
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHCCCHHHHHHHHHCCCE
VQIQVESTNRRVDPLTENIDIALRVRFPPLEDDGLVVKKLGHSAQRLVARADLAARLSAT
EEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHCCC
PSLDALSTLPSLDMPSSNHEHRWLLETHPGHYAEIRHTPRLISSDLDMMLQAALQGVGVA
CCHHHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCHH
QLPELIVREALQDGRLVELFSEHRPKCGIVHAVFASRRGLLPAVRGLLDVFETSFKELSL
HHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
SPDSLYCSLNRTNSRIQDH
CCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]