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The map label for this gene is yeaT [C]

Identifier: 66046182

GI number: 66046182

Start: 3539799

End: 3540698

Strand: Reverse

Name: yeaT [C]

Synonym: Psyr_2951

Alternate gene names: 66046182

Gene position: 3540698-3539799 (Counterclockwise)

Preceding gene: 66046197

Following gene: 66046176

Centisome position: 58.1

GC content: 58.11

Gene sequence:

>900_bases
ATGGATCGTGCACTGGAAATGCAGGTCTTCTGCACCGTTGTTGATAGGGGCAGTTTTGTCGGGGCAGCCGAGCCACTCGG
CATGTCCAAGGCAGCCATCTCGCGTTACGTGAGCACGCTGGAGGAGCGTCTCGGCGCGCGGTTGTTGCACCGCACGACTC
GGCGGCTTTCCTTGACGGATGAGGGGCGTCAGTTCTATCACCAGGCCCGTGAGGTGCTGGCCATGATGGATGAGGCAGAG
AAGGCTGTATCGTCTGCTTCGCCAGAGGCCAGCGGAGTGCTTCGAGTCAACGCCCCGGTCAGTTTTGGCGTGCTGCACCT
GGCTCCGATCTGGGCCGATTTCATGAGCGCCTACCCCAAGGTCGAGCTGGAAATCAGCTTGAGCGATCGTCTGGTGGATC
TGGTTGACGAAGGTATTGATGCAGCGGTGCGTATCGCACGCATGGATAATTCGTCGTTGGTGGGCAGACGCCTGGCCAGT
ACCCGGATGTGCCTGTGTGCGTCGCCTGCGTATCTGGCCAGCCACGCACCCATTGAAAAACTATCGGATGTGGCAGAGCA
CGGGGTAGTGGCCTACAGCAACTTTGCCAGCGGTACTGAATGGCAATTCGAGGGGCCCGAAGGCAGTGAAAGCGTGCACA
CGCGCTCGGTGGTTCGATGTAATAACGGGGATACCTGCCGCGCCATCACATTGGCCGGTGGTGGCATTTCGTTGCAGCCG
AGTTTTATGGTGGCGCAGGATCTACGCCGAGGCGATCTGGTCGAAATACTTCCCGAGTATCAATCGGTCGAGTTGGGGAT
CTACGTGGTGTATCCAACCCGTAAACATTTACCCGCGAAGGTAAGATCGCTGATCAACTTTCTGGTTGAGCGATTCGCCC
ATCCAGTATGGGAGCAGTGA

Upstream 100 bases:

>100_bases
CTCTGAGTTGAGTTGCGTTTCGATGGGCCCAGTTTATAGATTGCCAGGCAGGGATAAATCGGGCTGAATGTGATGAATAG
TTCCACTAAGTGAGACAATC

Downstream 100 bases:

>100_bases
CAGACCTTTGGAAAAAACCGGCTGATATAAGGGGCAGTGAAGGTATCAGCCGGATAAAAGGAGCGCTGTATCAGGAACGT
CCGTACACACAGAAACCCGG

Product: regulatory protein, LysR:LysR, substrate-binding

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MDRALEMQVFCTVVDRGSFVGAAEPLGMSKAAISRYVSTLEERLGARLLHRTTRRLSLTDEGRQFYHQAREVLAMMDEAE
KAVSSASPEASGVLRVNAPVSFGVLHLAPIWADFMSAYPKVELEISLSDRLVDLVDEGIDAAVRIARMDNSSLVGRRLAS
TRMCLCASPAYLASHAPIEKLSDVAEHGVVAYSNFASGTEWQFEGPEGSESVHTRSVVRCNNGDTCRAITLAGGGISLQP
SFMVAQDLRRGDLVEILPEYQSVELGIYVVYPTRKHLPAKVRSLINFLVERFAHPVWEQ

Sequences:

>Translated_299_residues
MDRALEMQVFCTVVDRGSFVGAAEPLGMSKAAISRYVSTLEERLGARLLHRTTRRLSLTDEGRQFYHQAREVLAMMDEAE
KAVSSASPEASGVLRVNAPVSFGVLHLAPIWADFMSAYPKVELEISLSDRLVDLVDEGIDAAVRIARMDNSSLVGRRLAS
TRMCLCASPAYLASHAPIEKLSDVAEHGVVAYSNFASGTEWQFEGPEGSESVHTRSVVRCNNGDTCRAITLAGGGISLQP
SFMVAQDLRRGDLVEILPEYQSVELGIYVVYPTRKHLPAKVRSLINFLVERFAHPVWEQ
>Mature_299_residues
MDRALEMQVFCTVVDRGSFVGAAEPLGMSKAAISRYVSTLEERLGARLLHRTTRRLSLTDEGRQFYHQAREVLAMMDEAE
KAVSSASPEASGVLRVNAPVSFGVLHLAPIWADFMSAYPKVELEISLSDRLVDLVDEGIDAAVRIARMDNSSLVGRRLAS
TRMCLCASPAYLASHAPIEKLSDVAEHGVVAYSNFASGTEWQFEGPEGSESVHTRSVVRCNNGDTCRAITLAGGGISLQP
SFMVAQDLRRGDLVEILPEYQSVELGIYVVYPTRKHLPAKVRSLINFLVERFAHPVWEQ

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=290, Percent_Identity=34.1379310344828, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI1786401, Length=287, Percent_Identity=31.7073170731707, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI145693193, Length=290, Percent_Identity=32.0689655172414, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1789639, Length=296, Percent_Identity=32.4324324324324, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1789440, Length=298, Percent_Identity=28.8590604026846, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1787128, Length=288, Percent_Identity=26.3888888888889, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1787589, Length=274, Percent_Identity=26.2773722627737, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1789173, Length=249, Percent_Identity=27.3092369477912, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1788706, Length=253, Percent_Identity=25.6916996047431, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790011, Length=299, Percent_Identity=20.066889632107, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI157672245, Length=225, Percent_Identity=25.7777777777778, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787806, Length=289, Percent_Identity=23.5294117647059, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87082132, Length=286, Percent_Identity=22.3776223776224, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787601, Length=132, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788748, Length=246, Percent_Identity=27.2357723577236, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788481, Length=286, Percent_Identity=21.6783216783217, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788297, Length=141, Percent_Identity=29.7872340425532, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32897; Mature: 32897

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRALEMQVFCTVVDRGSFVGAAEPLGMSKAAISRYVSTLEERLGARLLHRTTRRLSLTD
CCCCEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
EGRQFYHQAREVLAMMDEAEKAVSSASPEASGVLRVNAPVSFGVLHLAPIWADFMSAYPK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCC
VELEISLSDRLVDLVDEGIDAAVRIARMDNSSLVGRRLASTRMCLCASPAYLASHAPIEK
EEEEEEHHHHHHHHHHCCHHHHEEEEECCCCHHHHHHHHHHHHHEECCCHHHHCCCCHHH
LSDVAEHGVVAYSNFASGTEWQFEGPEGSESVHTRSVVRCNNGDTCRAITLAGGGISLQP
HHHHHHCCEEEEECCCCCCCEEECCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCEECC
SFMVAQDLRRGDLVEILPEYQSVELGIYVVYPTRKHLPAKVRSLINFLVERFAHPVWEQ
HHHHHHHCCCCCHHHHCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDRALEMQVFCTVVDRGSFVGAAEPLGMSKAAISRYVSTLEERLGARLLHRTTRRLSLTD
CCCCEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
EGRQFYHQAREVLAMMDEAEKAVSSASPEASGVLRVNAPVSFGVLHLAPIWADFMSAYPK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCC
VELEISLSDRLVDLVDEGIDAAVRIARMDNSSLVGRRLASTRMCLCASPAYLASHAPIEK
EEEEEEHHHHHHHHHHCCHHHHEEEEECCCCHHHHHHHHHHHHHEECCCHHHHCCCCHHH
LSDVAEHGVVAYSNFASGTEWQFEGPEGSESVHTRSVVRCNNGDTCRAITLAGGGISLQP
HHHHHHCCEEEEECCCCCCCEEECCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCEECC
SFMVAQDLRRGDLVEILPEYQSVELGIYVVYPTRKHLPAKVRSLINFLVERFAHPVWEQ
HHHHHHHCCCCCHHHHCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]