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The map label for this gene is nemA [C]

Identifier: 66046168

GI number: 66046168

Start: 3524525

End: 3525622

Strand: Reverse

Name: nemA [C]

Synonym: Psyr_2937

Alternate gene names: 66046168

Gene position: 3525622-3524525 (Counterclockwise)

Preceding gene: 66046170

Following gene: 66046163

Centisome position: 57.86

GC content: 62.39

Gene sequence:

>1098_bases
ATGAGCGAGACACACAAACACGTTACAGCCCCGTTCGACAGAGGTCATCTGAAACTGAAGAACAGGTTTGCGGTGGCGCC
CATGACACGCGTGAGCGCGGACGAGCAGGGCAGTGCAACGCAGGCCATGACGCGCTATTACGAGCGGTTCGCCAAGGGCG
GTTTTGGCCTGATCATCACCGAGGGCAACTACACCGATCGCCGTTTTTCCCAGGGCTACCGCTTCCAGCCCGGGCTCACT
GATGACGAGCAGGCGCAGGCATGGCGTGCCACGACCGATGCTGTGCACCGGCATGGCAGTGTCATGATTGCGCAGCTCAT
GCACGCCGGCGCACTCAGCCAGGCCAATCGCTTCGTCAGCGAGTCGGCGGGCCCTTCGGCCATTCGTCCCAAGGGCGAAC
AGATGGCGTTCTATTACGGCGTGGGCAGCTACCGGGTTCCGCGAGCGATGAGTGACAAGGATATTGCCGAGGCCATTGAC
GGGTTCGCCCGCGCGGCGCAACTGGCTATCGGCACTGCAGGCTTTGACGGTGTCGAGATACATGGTGCGAACGGCTACCT
GCTGGATCAGTTTCTGACCGACTACAGCAATCAGCGCACCGATCAGTGGGGCGGTGAGGTCGGACAGCGCCTGCGACTGA
CGCTCGAGGTCATCGCCGCAGTGAAAAAGGTCGCCGGGCAGGCACCGGTGGGCGTGCGTCTTTCACAAGGCAAGGTCAAC
GACTTTCAGCACAAATGGGCCGGGGGCGAGGGCGATGCCGAAGTCATTTTCGCTGCGCTGGCCGATGCCGGGGTCGATTA
CATCCATGTGACCGAACACGAAGCATGGCAGCCGGCCTTTACCCAGGGTCAAGCCAGCCTGGTCGAACTGGCCCGTCGCT
ACGCGCCGGATGTTGCGCTGATCGCCAATGGCGGCCTGCACGATCCGCAAAAAGCCGACCAGGTGCTTCGCCAGGGTGCG
GACATCATCGCAGTGGGCAAGGGTGCGCTGGCCAATCCGGACCTGCCAGAGTTGTTGCGTCAGGGCGCGCCACTGCGCGA
CTTCGATCCAGCCATTCTCGGGCCGATTGCCAACATCAAGGACAGTGAGCTGGTCTAG

Upstream 100 bases:

>100_bases
GCCGGGTTCCAATGGGCTGCGCCAGCCCTTACCGTTACTGCAGGTTCTGTCAATGACTGCGGGCAATCCGCAGCCGGTCT
GCACAGTGAGGATTTGAAGC

Downstream 100 bases:

>100_bases
TCGTTTGTGACGAACATCAGCGCTGGCTGAGCGTGCGCAATGCCTCGTTGATAGGTGTTGCGCCCTTAGTCAGTTCGATG
ATCTGGCGGGATATCCCGGG

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MSETHKHVTAPFDRGHLKLKNRFAVAPMTRVSADEQGSATQAMTRYYERFAKGGFGLIITEGNYTDRRFSQGYRFQPGLT
DDEQAQAWRATTDAVHRHGSVMIAQLMHAGALSQANRFVSESAGPSAIRPKGEQMAFYYGVGSYRVPRAMSDKDIAEAID
GFARAAQLAIGTAGFDGVEIHGANGYLLDQFLTDYSNQRTDQWGGEVGQRLRLTLEVIAAVKKVAGQAPVGVRLSQGKVN
DFQHKWAGGEGDAEVIFAALADAGVDYIHVTEHEAWQPAFTQGQASLVELARRYAPDVALIANGGLHDPQKADQVLRQGA
DIIAVGKGALANPDLPELLRQGAPLRDFDPAILGPIANIKDSELV

Sequences:

>Translated_365_residues
MSETHKHVTAPFDRGHLKLKNRFAVAPMTRVSADEQGSATQAMTRYYERFAKGGFGLIITEGNYTDRRFSQGYRFQPGLT
DDEQAQAWRATTDAVHRHGSVMIAQLMHAGALSQANRFVSESAGPSAIRPKGEQMAFYYGVGSYRVPRAMSDKDIAEAID
GFARAAQLAIGTAGFDGVEIHGANGYLLDQFLTDYSNQRTDQWGGEVGQRLRLTLEVIAAVKKVAGQAPVGVRLSQGKVN
DFQHKWAGGEGDAEVIFAALADAGVDYIHVTEHEAWQPAFTQGQASLVELARRYAPDVALIANGGLHDPQKADQVLRQGA
DIIAVGKGALANPDLPELLRQGAPLRDFDPAILGPIANIKDSELV
>Mature_364_residues
SETHKHVTAPFDRGHLKLKNRFAVAPMTRVSADEQGSATQAMTRYYERFAKGGFGLIITEGNYTDRRFSQGYRFQPGLTD
DEQAQAWRATTDAVHRHGSVMIAQLMHAGALSQANRFVSESAGPSAIRPKGEQMAFYYGVGSYRVPRAMSDKDIAEAIDG
FARAAQLAIGTAGFDGVEIHGANGYLLDQFLTDYSNQRTDQWGGEVGQRLRLTLEVIAAVKKVAGQAPVGVRLSQGKVND
FQHKWAGGEGDAEVIFAALADAGVDYIHVTEHEAWQPAFTQGQASLVELARRYAPDVALIANGGLHDPQKADQVLRQGAD
IIAVGKGALANPDLPELLRQGAPLRDFDPAILGPIANIKDSELV

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=358, Percent_Identity=33.7988826815642, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1789463, Length=343, Percent_Identity=30.9037900874636, Blast_Score=131, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17559804, Length=383, Percent_Identity=26.6318537859008, Blast_Score=110, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17540738, Length=350, Percent_Identity=28.8571428571429, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17565138, Length=368, Percent_Identity=27.445652173913, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17559802, Length=352, Percent_Identity=27.8409090909091, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17564188, Length=371, Percent_Identity=25.8760107816712, Blast_Score=98, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI72001454, Length=349, Percent_Identity=25.214899713467, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17566914, Length=273, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6321973, Length=365, Percent_Identity=29.3150684931507, Blast_Score=129, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6325086, Length=364, Percent_Identity=29.1208791208791, Blast_Score=128, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39514; Mature: 39383

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETHKHVTAPFDRGHLKLKNRFAVAPMTRVSADEQGSATQAMTRYYERFAKGGFGLIIT
CCCCCCCCCCCCCCCCEEECCEEEECCHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEE
EGNYTDRRFSQGYRFQPGLTDDEQAQAWRATTDAVHRHGSVMIAQLMHAGALSQANRFVS
CCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ESAGPSAIRPKGEQMAFYYGVGSYRVPRAMSDKDIAEAIDGFARAAQLAIGTAGFDGVEI
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
HGANGYLLDQFLTDYSNQRTDQWGGEVGQRLRLTLEVIAAVKKVAGQAPVGVRLSQGKVN
ECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCC
DFQHKWAGGEGDAEVIFAALADAGVDYIHVTEHEAWQPAFTQGQASLVELARRYAPDVAL
HHHHHCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCHHHCCHHHHHHHHHHHCCCEEE
IANGGLHDPQKADQVLRQGADIIAVGKGALANPDLPELLRQGAPLRDFDPAILGPIANIK
EECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHCCCCCCCCCHHHHCCCCCCC
DSELV
CCCCC
>Mature Secondary Structure 
SETHKHVTAPFDRGHLKLKNRFAVAPMTRVSADEQGSATQAMTRYYERFAKGGFGLIIT
CCCCCCCCCCCCCCCEEECCEEEECCHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEE
EGNYTDRRFSQGYRFQPGLTDDEQAQAWRATTDAVHRHGSVMIAQLMHAGALSQANRFVS
CCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ESAGPSAIRPKGEQMAFYYGVGSYRVPRAMSDKDIAEAIDGFARAAQLAIGTAGFDGVEI
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
HGANGYLLDQFLTDYSNQRTDQWGGEVGQRLRLTLEVIAAVKKVAGQAPVGVRLSQGKVN
ECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCC
DFQHKWAGGEGDAEVIFAALADAGVDYIHVTEHEAWQPAFTQGQASLVELARRYAPDVAL
HHHHHCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCHHHCCHHHHHHHHHHHCCCEEE
IANGGLHDPQKADQVLRQGADIIAVGKGALANPDLPELLRQGAPLRDFDPAILGPIANIK
EECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHCCCCCCCCCHHHHCCCCCCC
DSELV
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]