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The map label for this gene is nepI [H]

Identifier: 66046157

GI number: 66046157

Start: 3514660

End: 3515865

Strand: Reverse

Name: nepI [H]

Synonym: Psyr_2926

Alternate gene names: 66046157

Gene position: 3515865-3514660 (Counterclockwise)

Preceding gene: 66046161

Following gene: 66046156

Centisome position: 57.7

GC content: 61.36

Gene sequence:

>1206_bases
ATGACTACAGATGAGAAGCAGGGCAAAACACTCCCTCTGGGGGCGTTGCTGGCGCTGGCAATGACGGGGTTCATCTGCAT
CGTCACCGAGACCTTGCCTGCGGGTTTGTTGCCGCAGATCGCCGATGGGCTGGACGTGTCCGCATCATTTGCCGGGCAGA
TGGTGACTGTTTATGCGCTGGGTTCATTGCTGGCCGCCATTCCACTGACGATCGCGACGCAAAGCTGGCGGCGCAGAACG
GTCTTGCTGGTGACGATTATCGGCTTTCTGGTTTTCAACAGCGTCACCGCGTTGTCGTCCAGTTACGGACTGACCCTGAT
CGCAAGGTTTTTCGCAGGCGTCTCGGCGGGCCTGGCGTGGAGCCTCATCGCCGGTTACGCACGGCGCATGGTGGTCCCGC
AACTGCAAGGGCGCGCATTGGCCGTGGCGATGGTCGGCACGCCTATCGCCTTGTCGCTGGGCGTACCGCTGGGAACCTGG
CTTGGCGGGTTCATGGGCTGGCGCATGGCGTTTGGCCTGATGTCCGGGATGACCGTGCTGTTGATCATCTGGGTGCTGGT
CAAAGTGCCGGACTATCCGGGCAAGTCCTCCTCACAGCGCATGGCGTTACGTCAGGTCTTTTTCACGCCCGGCGTACGCT
CGGTGCTGGGCGTGGTTTTCACCTGGATGCTGGCTCATAACCTGCTTTACACCTACGTCGCTCCATTCGTTTCCGCGGCG
GGGCTGGCGAATGATGTGGACCTGGTATTGCTGGCATTCGGGGTCGCCGCACTGGCTGGCATCTGGGTTACGGGACGGTT
GGTTGATCGCCATCTGCGCAAGACGGTGCTTGCCAGCCTGGCAACCTTCGCAGCGGTGTCAGTGTTTTTCGGCGTGTTCT
CCGGCTCTGCAACGGCCATTTACATCGGTGCTTTTATCTGGGGCTTGACCTTCGGTGGCGCCGCCACACTGCTGCAAACC
GCGCTCGCCGATTCGGCAGGCGCGGGCGCAGATGTGGCGCTTTCGATGAACGTCGTGGTCTGGAACAGCGCGATCGCAGG
CGGCGGGTTACTGGGTGGCGTGTTGCTTGGCCAATGGGGTGCGGGATCCTTTCCTTGGGTCTTGCTGATGCTGTTGTTGC
TAAGTCTGGTGATCGCACTGCGCGCAGGTGCTCATGGTTTTGCACGGGGCAGCCGGCTGGACCGGGAGCATGTTTTGGCT
GGGTGA

Upstream 100 bases:

>100_bases
CTTCGGAATTAATTAAATACTAATCGATACATAAGTCGTTGACCACCTGACTCCATCTCCCTATATTTGTACCGATCAGT
ATATTAGTGAGGGTTTTTCA

Downstream 100 bases:

>100_bases
TCTGATCAAGACCCGGAATATGGTCGTCAAGCTGCTTGCTGATGCAGGGGGTTAATTCTATAATTTTGTGAATTAAATGG
CTGAATCGAGCTTTATACAC

Product: major facilitator transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MTTDEKQGKTLPLGALLALAMTGFICIVTETLPAGLLPQIADGLDVSASFAGQMVTVYALGSLLAAIPLTIATQSWRRRT
VLLVTIIGFLVFNSVTALSSSYGLTLIARFFAGVSAGLAWSLIAGYARRMVVPQLQGRALAVAMVGTPIALSLGVPLGTW
LGGFMGWRMAFGLMSGMTVLLIIWVLVKVPDYPGKSSSQRMALRQVFFTPGVRSVLGVVFTWMLAHNLLYTYVAPFVSAA
GLANDVDLVLLAFGVAALAGIWVTGRLVDRHLRKTVLASLATFAAVSVFFGVFSGSATAIYIGAFIWGLTFGGAATLLQT
ALADSAGAGADVALSMNVVVWNSAIAGGGLLGGVLLGQWGAGSFPWVLLMLLLLSLVIALRAGAHGFARGSRLDREHVLA
G

Sequences:

>Translated_401_residues
MTTDEKQGKTLPLGALLALAMTGFICIVTETLPAGLLPQIADGLDVSASFAGQMVTVYALGSLLAAIPLTIATQSWRRRT
VLLVTIIGFLVFNSVTALSSSYGLTLIARFFAGVSAGLAWSLIAGYARRMVVPQLQGRALAVAMVGTPIALSLGVPLGTW
LGGFMGWRMAFGLMSGMTVLLIIWVLVKVPDYPGKSSSQRMALRQVFFTPGVRSVLGVVFTWMLAHNLLYTYVAPFVSAA
GLANDVDLVLLAFGVAALAGIWVTGRLVDRHLRKTVLASLATFAAVSVFFGVFSGSATAIYIGAFIWGLTFGGAATLLQT
ALADSAGAGADVALSMNVVVWNSAIAGGGLLGGVLLGQWGAGSFPWVLLMLLLLSLVIALRAGAHGFARGSRLDREHVLA
G
>Mature_400_residues
TTDEKQGKTLPLGALLALAMTGFICIVTETLPAGLLPQIADGLDVSASFAGQMVTVYALGSLLAAIPLTIATQSWRRRTV
LLVTIIGFLVFNSVTALSSSYGLTLIARFFAGVSAGLAWSLIAGYARRMVVPQLQGRALAVAMVGTPIALSLGVPLGTWL
GGFMGWRMAFGLMSGMTVLLIIWVLVKVPDYPGKSSSQRMALRQVFFTPGVRSVLGVVFTWMLAHNLLYTYVAPFVSAAG
LANDVDLVLLAFGVAALAGIWVTGRLVDRHLRKTVLASLATFAAVSVFFGVFSGSATAIYIGAFIWGLTFGGAATLLQTA
LADSAGAGADVALSMNVVVWNSAIAGGGLLGGVLLGQWGAGSFPWVLLMLLLLSLVIALRAGAHGFARGSRLDREHVLAG

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and especially inosine. Involved in the resistance to 6-mercaptopurine [H]

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. NepI (TC 2.A.1.2.26) subfamily [H]

Homologues:

Organism=Escherichia coli, GI145693198, Length=363, Percent_Identity=33.0578512396694, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1787808, Length=238, Percent_Identity=29.4117647058824, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787947, Length=270, Percent_Identity=30.3703703703704, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1786595, Length=338, Percent_Identity=24.8520710059172, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41806; Mature: 41675

Theoretical pI: Translated: 10.69; Mature: 10.69

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCHHHCCCC
>Mature Secondary Structure 
TTDEKQGKTLPLGALLALAMTGFICIVTETLPAGLLPQIADGLDVSASFAGQMVTVYAL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHHHH
GSLLAAIPLTIATQSWRRRTVLLVTIIGFLVFNSVTALSSSYGLTLIARFFAGVSAGLAW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
SLIAGYARRMVVPQLQGRALAVAMVGTPIALSLGVPLGTWLGGFMGWRMAFGLMSGMTVL
HHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
LIIWVLVKVPDYPGKSSSQRMALRQVFFTPGVRSVLGVVFTWMLAHNLLYTYVAPFVSAA
HHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLANDVDLVLLAFGVAALAGIWVTGRLVDRHLRKTVLASLATFAAVSVFFGVFSGSATAI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
YIGAFIWGLTFGGAATLLQTALADSAGAGADVALSMNVVVWNSAIAGGGLLGGVLLGQWG
HHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHCCCC
AGSFPWVLLMLLLLSLVIALRAGAHGFARGSRLDREHVLAG
CCCCHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA