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The map label for this gene is mexA [H]

Identifier: 66046099

GI number: 66046099

Start: 3440050

End: 3441252

Strand: Reverse

Name: mexA [H]

Synonym: Psyr_2866

Alternate gene names: 66046099

Gene position: 3441252-3440050 (Counterclockwise)

Preceding gene: 66046102

Following gene: 66046098

Centisome position: 56.47

GC content: 62.51

Gene sequence:

>1203_bases
ATGTCGAGCAAGATTTTCGCCAAATACCTGATGACTTTGACCTTTGTCATCTACGCAGCCCTTATCGGTCTTCTGAGCGG
TTGTTCTGACGGTGATTCGCCGCCTGCTGCGGAGAAACCGAGCGTGTCGGTGGTCACCGTCAAACCGCGCAGCCAGTCGC
TGACCACCGAGCTGGCGGGGCGCACCAAAGCGTTCATGGTTGCCGAGATACGGCCCCAGGTCAGTGGCATCGTGCAGCAG
CGACTGTTCGTCGAAGGCGCCGAAATCAAGGCTGGTCAGCCGTTGTACCAACTCGACAGTGCGACCTACCAGGCGGCTCT
GGCAGAATCGCAGGCCACGCTCGCCAAGTCCCGTACCACGCTCAAGTCCGCACAGGCCACCGCCAAACGCGATGTGCAGT
TGGCCAGGATCGACGCCATCAGCCAGCAGGATAAAGAAGACGCCGAGGCCAGCCTGCTGACTGCCGCCGCCGAGGTGAAA
GTGGCGGAGGCCGATGTGCAGACCGCACGCATCAATCTGGCCTACACACGGATTACCGCGCCCATCAGCGGCCGCATCGA
GACCTCGACCGTGACCCCCGGTGCATTGGTGGTGGCCCAGCAGGACACCGCGCTGACCACGGTTCAGCAGCTCGACCCGA
TTTATGTCGATGTCACCCAGTCCACCACCGAGTTGCTGCGTCTCAAGCGCGACCTGGCCAGCGGCAAGCTGCAAACCAAC
AGCGACGGGGAAGCCCGAATCACCCTCAAGCTGGATGACGGCAGCACCTACAACCAGGATGGCCGCCTGCAATTCAGCGG
CGTCAGCGTCAATGAAGGCACCGGCACCGTGACGCTGCGTGCCAGCTTTGCCAACCCAGAACGGCTGTTGCTGCCCGGCA
TGTACGTGGTGGCGGTGCTGGAACAGGCGCAGGATCAGAATGCGATCCTGATACCGCAAAAGGCCGTCACCCGCAGTGCC
AGTGGCCAGACCACGGTGTTGCTGGTGGTTGACGGCAAGATCGAGCAGCGGGTGATCAGCGTCGACCGCACGGTGGGCAA
CCAGTGGTGGGTGAGCAGCGGGCTCAAGGCCAATGATCAGGTGGTGGTCGAAGGCGGGCAGAAAACCCCGGTCGGCACCG
AGGTTAGCGTGCAGAACGCTGATAACGCCGATTCGCCAGCATCTGGTGCTGCGGCCACTGCCGCTACCGTGAAGGAGGGC
TGA

Upstream 100 bases:

>100_bases
GTGACAGCAAGTTTCAAGTCAACGCGGCAATCTTCAATTTTCCTGCACATTTGCCTCGCACTCTGACGTCAGGCTTTCGC
CACAACGTCCTGAGGCAATC

Downstream 100 bases:

>100_bases
ACATGGCTCGTTTCTTCATCGATCGGCCCATCTTCGCCTGGGTCATTGCCATCTGCATCATGTTCGCCGGCGGGCTGTCC
ATTAGCCAGCTGCCCCTTGA

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MSSKIFAKYLMTLTFVIYAALIGLLSGCSDGDSPPAAEKPSVSVVTVKPRSQSLTTELAGRTKAFMVAEIRPQVSGIVQQ
RLFVEGAEIKAGQPLYQLDSATYQAALAESQATLAKSRTTLKSAQATAKRDVQLARIDAISQQDKEDAEASLLTAAAEVK
VAEADVQTARINLAYTRITAPISGRIETSTVTPGALVVAQQDTALTTVQQLDPIYVDVTQSTTELLRLKRDLASGKLQTN
SDGEARITLKLDDGSTYNQDGRLQFSGVSVNEGTGTVTLRASFANPERLLLPGMYVVAVLEQAQDQNAILIPQKAVTRSA
SGQTTVLLVVDGKIEQRVISVDRTVGNQWWVSSGLKANDQVVVEGGQKTPVGTEVSVQNADNADSPASGAAATAATVKEG

Sequences:

>Translated_400_residues
MSSKIFAKYLMTLTFVIYAALIGLLSGCSDGDSPPAAEKPSVSVVTVKPRSQSLTTELAGRTKAFMVAEIRPQVSGIVQQ
RLFVEGAEIKAGQPLYQLDSATYQAALAESQATLAKSRTTLKSAQATAKRDVQLARIDAISQQDKEDAEASLLTAAAEVK
VAEADVQTARINLAYTRITAPISGRIETSTVTPGALVVAQQDTALTTVQQLDPIYVDVTQSTTELLRLKRDLASGKLQTN
SDGEARITLKLDDGSTYNQDGRLQFSGVSVNEGTGTVTLRASFANPERLLLPGMYVVAVLEQAQDQNAILIPQKAVTRSA
SGQTTVLLVVDGKIEQRVISVDRTVGNQWWVSSGLKANDQVVVEGGQKTPVGTEVSVQNADNADSPASGAAATAATVKEG
>Mature_399_residues
SSKIFAKYLMTLTFVIYAALIGLLSGCSDGDSPPAAEKPSVSVVTVKPRSQSLTTELAGRTKAFMVAEIRPQVSGIVQQR
LFVEGAEIKAGQPLYQLDSATYQAALAESQATLAKSRTTLKSAQATAKRDVQLARIDAISQQDKEDAEASLLTAAAEVKV
AEADVQTARINLAYTRITAPISGRIETSTVTPGALVVAQQDTALTTVQQLDPIYVDVTQSTTELLRLKRDLASGKLQTNS
DGEARITLKLDDGSTYNQDGRLQFSGVSVNEGTGTVTLRASFANPERLLLPGMYVVAVLEQAQDQNAILIPQKAVTRSAS
GQTTVLLVVDGKIEQRVISVDRTVGNQWWVSSGLKANDQVVVEGGQKTPVGTEVSVQNADNADSPASGAAATAATVKEG

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=370, Percent_Identity=50.2702702702703, Blast_Score=317, Evalue=9e-88,
Organism=Escherichia coli, GI1789665, Length=388, Percent_Identity=45.8762886597938, Blast_Score=311, Evalue=4e-86,
Organism=Escherichia coli, GI1789929, Length=363, Percent_Identity=43.801652892562, Blast_Score=284, Evalue=8e-78,
Organism=Escherichia coli, GI87082036, Length=321, Percent_Identity=26.791277258567, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI87081796, Length=388, Percent_Identity=25, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42281; Mature: 42150

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKIFAKYLMTLTFVIYAALIGLLSGCSDGDSPPAAEKPSVSVVTVKPRSQSLTTELAG
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHCC
RTKAFMVAEIRPQVSGIVQQRLFVEGAEIKAGQPLYQLDSATYQAALAESQATLAKSRTT
CCEEEEEEECCHHHHHHHHHHHHEECCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHH
LKSAQATAKRDVQLARIDAISQQDKEDAEASLLTAAAEVKVAEADVQTARINLAYTRITA
HHHHHHHHHHCEEEEEHHHHCCCCHHHHHHHHHHHHHHEEEECCCCEEEEEEEEEEEEEC
PISGRIETSTVTPGALVVAQQDTALTTVQQLDPIYVDVTQSTTELLRLKRDLASGKLQTN
CCCCCEEECEECCCEEEEEECCCHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCEEEC
SDGEARITLKLDDGSTYNQDGRLQFSGVSVNEGTGTVTLRASFANPERLLLPGMYVVAVL
CCCCEEEEEEECCCCCCCCCCEEEEEEEEEECCCCEEEEEEECCCCCEEECCHHHHHHHH
EQAQDQNAILIPQKAVTRSASGQTTVLLVVDGKIEQRVISVDRTVGNQWWVSSGLKANDQ
HHHCCCCEEEECHHHHHCCCCCCEEEEEEECCCHHHHHEEHHHHCCCCEEECCCCCCCCE
VVVEGGQKTPVGTEVSVQNADNADSPASGAAATAATVKEG
EEEECCCCCCCCCEEEECCCCCCCCCCCCCHHEEEECCCC
>Mature Secondary Structure 
SSKIFAKYLMTLTFVIYAALIGLLSGCSDGDSPPAAEKPSVSVVTVKPRSQSLTTELAG
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHCC
RTKAFMVAEIRPQVSGIVQQRLFVEGAEIKAGQPLYQLDSATYQAALAESQATLAKSRTT
CCEEEEEEECCHHHHHHHHHHHHEECCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHH
LKSAQATAKRDVQLARIDAISQQDKEDAEASLLTAAAEVKVAEADVQTARINLAYTRITA
HHHHHHHHHHCEEEEEHHHHCCCCHHHHHHHHHHHHHHEEEECCCCEEEEEEEEEEEEEC
PISGRIETSTVTPGALVVAQQDTALTTVQQLDPIYVDVTQSTTELLRLKRDLASGKLQTN
CCCCCEEECEECCCEEEEEECCCHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCEEEC
SDGEARITLKLDDGSTYNQDGRLQFSGVSVNEGTGTVTLRASFANPERLLLPGMYVVAVL
CCCCEEEEEEECCCCCCCCCCEEEEEEEEEECCCCEEEEEEECCCCCEEECCHHHHHHHH
EQAQDQNAILIPQKAVTRSASGQTTVLLVVDGKIEQRVISVDRTVGNQWWVSSGLKANDQ
HHHCCCCEEEECHHHHHCCCCCCEEEEEEECCCHHHHHEEHHHHCCCCEEECCCCCCCCE
VVVEGGQKTPVGTEVSVQNADNADSPASGAAATAATVKEG
EEEECCCCCCCCCEEEECCCCCCCCCCCCCHHEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]