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The map label for this gene is oprM [H]

Identifier: 66046097

GI number: 66046097

Start: 3435495

End: 3436901

Strand: Reverse

Name: oprM [H]

Synonym: Psyr_2864

Alternate gene names: 66046097

Gene position: 3436901-3435495 (Counterclockwise)

Preceding gene: 66046098

Following gene: 66046096

Centisome position: 56.4

GC content: 62.83

Gene sequence:

>1407_bases
ATGCGTCTTTTCTGGCCTTCGCTGGCTGTCACGGCGCTGCTTGCGGGCTGCGTCAACCTGGCCCCGGACTATCAGCGCCC
TGAAGCGCCGGTCTCAAGCCAATGGCTGCCGGGTACTGCTGGCCAACCGGCTGCGTCGGGCAGCGTGCCCACGGACATCG
AATGGCAGAATTTTTTCATCGATAACCGCCTGGTGCGCCTGCAGACCCTGGCGCTGGTGAATAACCGCGACCTGCGCCTG
GCCAGCCTCAACGTCGAGAAAGCGCAGGCGCAATATCGCATCGAGCGGGCGGCGAGCCTGCCGACCATTGATGCCAGTGG
CAGCGGCACGCATACACGCACGCCGTCATCGACCTCGATGACCGGCAAGCCGTCGATCAGCCACGAGTACAGCGCGCAGT
TGGGCTTGAGCAGCTATGAGCTGGATGTGTTCGGGCGTATCCAGAACCTTCAGGACGAAGCGCTTGAGGATTATCTGGCG
TTGACCGAAACCCGGCGCAGCACGCAGATCAGCCTGATCGCCGAAGTGGCGACCGGCTGGCTGACCCTGGCCGCCGACAA
CCAGCTTCTGAAACTCGCCGAAGACACGTTGGCCAGCCAGCAGAAAAGCTACGACCTGACCAGAAGCAGCCATGCGCTGG
GCGGTTCTTCCGGGCTGGAAGTGGCTCAGGCGCAGACAACGGTCGAATCGGCGAGGGGCGATGTCGCGCAATATAAAAGC
CAGATTCTTCAGGACCGTAACGCCTTGCGCCTGCTGGTCGGCAGCGATCTGCCGGAGGAACTGCTGCCCAATGCCGACAC
GCAGTCGGTGGCGCAATTGGTGCAGGTGCCGGACGGCCTGCCTTCCAGCCTCCTGCAACGCCGCCCGGACGTGCTGAGTG
CCGAACACACACTTAAAGCCGCGAACATTGATATCGGTGCAGCGCGTGCGGCGTTCTTCCCCAGTATCACCCTGACTGCC
AGTGCAGGCTCTTCCAGCACCAGCCTGTCAGGCCTGTTCAAGTCCGGAACCGGCGCGTGGAGCTTTGCGCCAAGTATCAG
CGTGCCGATTTTCGATGGCGGCGCCAATCGGGCCACGCTGGACTCGGCCAAGGTCGAGAACCAGATCCAGATTCAGACCT
ATCAGCAGACGATCCAGACCGCATTCAAGGAAGTGGCCGATGCCTTGGCGGTGCGCAGCACGCTGGATGAGCGGCTTGCC
GCGCAGCAGGCCTACACCGATGCCAGCCGCAGAAGCTACGAACTGGCCGACGCCCTGTACCGCGGCGGATCGCAGAGTTA
TCTGGAGGCGCTCGAGTCGCAACGCTCGCTCTACAGTGCCGAGCAGGACCTGATTTCCCTGCGCCTCACAGAGCAGAGCA
ACCGCGTCACGCTCTACAAGGTCATGGGCGGCGGATGGAATCGGTGA

Upstream 100 bases:

>100_bases
TTTTCCTGGTGCCGCTGTTCTTCGTCGAAGTGCGTCGCCGCTTCAGTCGTTCGTCAGGCAATGCTCCGGCGGCTCAACCC
ACATCTACAGGTGAAGCATG

Downstream 100 bases:

>100_bases
TGGTTTTTTTCTACTGGCTTTTGAATAATCGCCAGGCTTAAACGTTTTACCCTTACGCACAGTGCGGCCATTTATGGGTT
CGCACTGTGGTTTTGATCCT

Product: RND efflux system, outer membrane lipoprotein, NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MRLFWPSLAVTALLAGCVNLAPDYQRPEAPVSSQWLPGTAGQPAASGSVPTDIEWQNFFIDNRLVRLQTLALVNNRDLRL
ASLNVEKAQAQYRIERAASLPTIDASGSGTHTRTPSSTSMTGKPSISHEYSAQLGLSSYELDVFGRIQNLQDEALEDYLA
LTETRRSTQISLIAEVATGWLTLAADNQLLKLAEDTLASQQKSYDLTRSSHALGGSSGLEVAQAQTTVESARGDVAQYKS
QILQDRNALRLLVGSDLPEELLPNADTQSVAQLVQVPDGLPSSLLQRRPDVLSAEHTLKAANIDIGAARAAFFPSITLTA
SAGSSSTSLSGLFKSGTGAWSFAPSISVPIFDGGANRATLDSAKVENQIQIQTYQQTIQTAFKEVADALAVRSTLDERLA
AQQAYTDASRRSYELADALYRGGSQSYLEALESQRSLYSAEQDLISLRLTEQSNRVTLYKVMGGGWNR

Sequences:

>Translated_468_residues
MRLFWPSLAVTALLAGCVNLAPDYQRPEAPVSSQWLPGTAGQPAASGSVPTDIEWQNFFIDNRLVRLQTLALVNNRDLRL
ASLNVEKAQAQYRIERAASLPTIDASGSGTHTRTPSSTSMTGKPSISHEYSAQLGLSSYELDVFGRIQNLQDEALEDYLA
LTETRRSTQISLIAEVATGWLTLAADNQLLKLAEDTLASQQKSYDLTRSSHALGGSSGLEVAQAQTTVESARGDVAQYKS
QILQDRNALRLLVGSDLPEELLPNADTQSVAQLVQVPDGLPSSLLQRRPDVLSAEHTLKAANIDIGAARAAFFPSITLTA
SAGSSSTSLSGLFKSGTGAWSFAPSISVPIFDGGANRATLDSAKVENQIQIQTYQQTIQTAFKEVADALAVRSTLDERLA
AQQAYTDASRRSYELADALYRGGSQSYLEALESQRSLYSAEQDLISLRLTEQSNRVTLYKVMGGGWNR
>Mature_468_residues
MRLFWPSLAVTALLAGCVNLAPDYQRPEAPVSSQWLPGTAGQPAASGSVPTDIEWQNFFIDNRLVRLQTLALVNNRDLRL
ASLNVEKAQAQYRIERAASLPTIDASGSGTHTRTPSSTSMTGKPSISHEYSAQLGLSSYELDVFGRIQNLQDEALEDYLA
LTETRRSTQISLIAEVATGWLTLAADNQLLKLAEDTLASQQKSYDLTRSSHALGGSSGLEVAQAQTTVESARGDVAQYKS
QILQDRNALRLLVGSDLPEELLPNADTQSVAQLVQVPDGLPSSLLQRRPDVLSAEHTLKAANIDIGAARAAFFPSITLTA
SAGSSSTSLSGLFKSGTGAWSFAPSISVPIFDGGANRATLDSAKVENQIQIQTYQQTIQTAFKEVADALAVRSTLDERLA
AQQAYTDASRRSYELADALYRGGSQSYLEALESQRSLYSAEQDLISLRLTEQSNRVTLYKVMGGGWNR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=460, Percent_Identity=41.304347826087, Blast_Score=349, Evalue=3e-97,
Organism=Escherichia coli, GI2367348, Length=494, Percent_Identity=27.9352226720648, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 50534; Mature: 50534

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFWPSLAVTALLAGCVNLAPDYQRPEAPVSSQWLPGTAGQPAASGSVPTDIEWQNFFI
CCEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEE
DNRLVRLQTLALVNNRDLRLASLNVEKAQAQYRIERAASLPTIDASGSGTHTRTPSSTSM
CCHHEEHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
TGKPSISHEYSAQLGLSSYELDVFGRIQNLQDEALEDYLALTETRRSTQISLIAEVATGW
CCCCCCCCCHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCE
LTLAADNQLLKLAEDTLASQQKSYDLTRSSHALGGSSGLEVAQAQTTVESARGDVAQYKS
EEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHH
QILQDRNALRLLVGSDLPEELLPNADTQSVAQLVQVPDGLPSSLLQRRPDVLSAEHTLKA
HHHHCCCCEEEEECCCCCHHHCCCCCHHHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHEE
ANIDIGAARAAFFPSITLTASAGSSSTSLSGLFKSGTGAWSFAPSISVPIFDGGANRATL
CCCCCCHHHHHHCCCEEEEECCCCCCCHHHHHHHCCCCCEEECCCCCEEEECCCCCCCCC
DSAKVENQIQIQTYQQTIQTAFKEVADALAVRSTLDERLAAQQAYTDASRRSYELADALY
CHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGGSQSYLEALESQRSLYSAEQDLISLRLTEQSNRVTLYKVMGGGWNR
CCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEEECCCCCC
>Mature Secondary Structure
MRLFWPSLAVTALLAGCVNLAPDYQRPEAPVSSQWLPGTAGQPAASGSVPTDIEWQNFFI
CCEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEE
DNRLVRLQTLALVNNRDLRLASLNVEKAQAQYRIERAASLPTIDASGSGTHTRTPSSTSM
CCHHEEHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
TGKPSISHEYSAQLGLSSYELDVFGRIQNLQDEALEDYLALTETRRSTQISLIAEVATGW
CCCCCCCCCHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCE
LTLAADNQLLKLAEDTLASQQKSYDLTRSSHALGGSSGLEVAQAQTTVESARGDVAQYKS
EEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHH
QILQDRNALRLLVGSDLPEELLPNADTQSVAQLVQVPDGLPSSLLQRRPDVLSAEHTLKA
HHHHCCCCEEEEECCCCCHHHCCCCCHHHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHEE
ANIDIGAARAAFFPSITLTASAGSSSTSLSGLFKSGTGAWSFAPSISVPIFDGGANRATL
CCCCCCHHHHHHCCCEEEEECCCCCCCHHHHHHHCCCCCEEECCCCCEEEECCCCCCCCC
DSAKVENQIQIQTYQQTIQTAFKEVADALAVRSTLDERLAAQQAYTDASRRSYELADALY
CHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGGSQSYLEALESQRSLYSAEQDLISLRLTEQSNRVTLYKVMGGGWNR
CCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]