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The map label for this gene is pcbC [H]

Identifier: 66046091

GI number: 66046091

Start: 3429258

End: 3430271

Strand: Reverse

Name: pcbC [H]

Synonym: Psyr_2858

Alternate gene names: 66046091

Gene position: 3430271-3429258 (Counterclockwise)

Preceding gene: 66046092

Following gene: 66046090

Centisome position: 56.29

GC content: 56.21

Gene sequence:

>1014_bases
ATGACTCTGCACTATGTGCCCATTATCAACCTGGCCCCCTATTTTTCAGAAGACCCGGACGGCAAGGCTGCCGTAGCCAG
GGCAGTCAATCAGGCGTGTCGGGATATCGGGTTTCTGGTCATCACCGAGCACCAGATTCCCGGAGAACTTATCGACAGTG
TCTCGCGTTTGACCCGGCAATTTTTTGATTTGCCGCTGGCGGAAAAGCGCAAGGTCGATCGGCCCAGCCCGGAAATGGTG
CGTGGTTACAGCGCCGTGGCCGAGGAGAGCCTTTCCTATTCTCTGGAGGAAAGTGCGCCGGGGGATCTCAAGGAGTCTTT
CTCCATCGGGCCGAGCGATGTGCCGGATGAAGATTATTATCACAATGCCCAGGCCGGGTCGCACTTCGCACCGAATGTCT
GGCCTGCGCAAGACCTGCTGCCCGGATTCAAGGAGTCTTACCAGGCCTATTTCGATGCCATGAGCGAATTGGCTCAATCG
TTGATGCGCCTGTTTGCCCTGGCATTGGAACTGGATGAGTGTTTTTTCGATGACAAGATCGACCGGCATATCAGCATGTT
CCGCAGTCTGAGTTATCCGGATATCAAGACTGAAGTCGAGGCTGGCCAGTTACGCGCCAGTGCGCATACCGACTATGGCA
GCTTGACCATCGTGCGCCCTGATAACGCGCTGGGCGGCTTGCAAGTACGTAATCGCCAGGGCGAGTGGGTCGATGTGCCG
TATGTCGAGAATGGTTTTGTGGTCAACATTGGCGATCTGATGATGCAGTGGACCAACGATCAGTGGATTTCCACGCTGCA
CCGAGTGGTCAATCCACCGATGACCAGCGAGCAGGACAACCGCCGACAGTCCCTGGTGTTTTTCCACCAACCCAACTACG
ACACGTTGATTCAATGCCTGCCAGGTTGCCTGCAGCCTGGCGCCACGCCTCGGCATGCGCCGGTTACATCAGGCGATCAT
TTGCTGTCCAAGTTCGTCAAGCAGACCACTTTTGGCGGCAGCAAGGTGGCCTGA

Upstream 100 bases:

>100_bases
ATGAATTCTTCCAGTGGCATGCTTTCTGCTCCTGCCTCGCTGACCTTCACCCTTGTTGGTGAGCCTTTCCAGCAGCGTTT
CAGCGAGAGGAAGCAGCCGT

Downstream 100 bases:

>100_bases
CATGAGCACACTTTCATACGTCAACGTGTTCGCGCAGGACATCGTCCGCCTGAGCACCTTCTACTCGAACCTGTTCGGCT
TTCGCGAAATCGAGGCCATC

Product: 2OG-Fe(II) oxygenase family protein

Products: NA

Alternate protein names: IPNS [H]

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MTLHYVPIINLAPYFSEDPDGKAAVARAVNQACRDIGFLVITEHQIPGELIDSVSRLTRQFFDLPLAEKRKVDRPSPEMV
RGYSAVAEESLSYSLEESAPGDLKESFSIGPSDVPDEDYYHNAQAGSHFAPNVWPAQDLLPGFKESYQAYFDAMSELAQS
LMRLFALALELDECFFDDKIDRHISMFRSLSYPDIKTEVEAGQLRASAHTDYGSLTIVRPDNALGGLQVRNRQGEWVDVP
YVENGFVVNIGDLMMQWTNDQWISTLHRVVNPPMTSEQDNRRQSLVFFHQPNYDTLIQCLPGCLQPGATPRHAPVTSGDH
LLSKFVKQTTFGGSKVA

Sequences:

>Translated_337_residues
MTLHYVPIINLAPYFSEDPDGKAAVARAVNQACRDIGFLVITEHQIPGELIDSVSRLTRQFFDLPLAEKRKVDRPSPEMV
RGYSAVAEESLSYSLEESAPGDLKESFSIGPSDVPDEDYYHNAQAGSHFAPNVWPAQDLLPGFKESYQAYFDAMSELAQS
LMRLFALALELDECFFDDKIDRHISMFRSLSYPDIKTEVEAGQLRASAHTDYGSLTIVRPDNALGGLQVRNRQGEWVDVP
YVENGFVVNIGDLMMQWTNDQWISTLHRVVNPPMTSEQDNRRQSLVFFHQPNYDTLIQCLPGCLQPGATPRHAPVTSGDH
LLSKFVKQTTFGGSKVA
>Mature_336_residues
TLHYVPIINLAPYFSEDPDGKAAVARAVNQACRDIGFLVITEHQIPGELIDSVSRLTRQFFDLPLAEKRKVDRPSPEMVR
GYSAVAEESLSYSLEESAPGDLKESFSIGPSDVPDEDYYHNAQAGSHFAPNVWPAQDLLPGFKESYQAYFDAMSELAQSL
MRLFALALELDECFFDDKIDRHISMFRSLSYPDIKTEVEAGQLRASAHTDYGSLTIVRPDNALGGLQVRNRQGEWVDVPY
VENGFVVNIGDLMMQWTNDQWISTLHRVVNPPMTSEQDNRRQSLVFFHQPNYDTLIQCLPGCLQPGATPRHAPVTSGDHL
LSKFVKQTTFGGSKVA

Specific function: Removes, in the presence of oxygen, 4 hydrogen atoms from delta-L-(alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) to form the azetidinone and thiazolidine rings of isopenicillin [H]

COG id: COG3491

COG function: function code R; Isopenicillin N synthase and related dioxygenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Drosophila melanogaster, GI28572124, Length=307, Percent_Identity=27.3615635179153, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI85816264, Length=305, Percent_Identity=24.9180327868852, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002057
- InterPro:   IPR002283
- InterPro:   IPR005123 [H]

Pfam domain/function: PF03171 2OG-FeII_Oxy [H]

EC number: =1.21.3.1 [H]

Molecular weight: Translated: 37746; Mature: 37615

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLHYVPIINLAPYFSEDPDGKAAVARAVNQACRDIGFLVITEHQIPGELIDSVSRLTRQ
CEEEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHH
FFDLPLAEKRKVDRPSPEMVRGYSAVAEESLSYSLEESAPGDLKESFSIGPSDVPDEDYY
HHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCC
HNAQAGSHFAPNVWPAQDLLPGFKESYQAYFDAMSELAQSLMRLFALALELDECFFDDKI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRHISMFRSLSYPDIKTEVEAGQLRASAHTDYGSLTIVRPDNALGGLQVRNRQGEWVDVP
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEECCCCCEEECC
YVENGFVVNIGDLMMQWTNDQWISTLHRVVNPPMTSEQDNRRQSLVFFHQPNYDTLIQCL
EECCCEEEEHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCHHHHHHHH
PGCLQPGATPRHAPVTSGDHLLSKFVKQTTFGGSKVA
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TLHYVPIINLAPYFSEDPDGKAAVARAVNQACRDIGFLVITEHQIPGELIDSVSRLTRQ
EEEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHH
FFDLPLAEKRKVDRPSPEMVRGYSAVAEESLSYSLEESAPGDLKESFSIGPSDVPDEDYY
HHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCC
HNAQAGSHFAPNVWPAQDLLPGFKESYQAYFDAMSELAQSLMRLFALALELDECFFDDKI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRHISMFRSLSYPDIKTEVEAGQLRASAHTDYGSLTIVRPDNALGGLQVRNRQGEWVDVP
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEECCCCCEEECC
YVENGFVVNIGDLMMQWTNDQWISTLHRVVNPPMTSEQDNRRQSLVFFHQPNYDTLIQCL
EECCCEEEEHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCHHHHHHHH
PGCLQPGATPRHAPVTSGDHLLSKFVKQTTFGGSKVA
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1956290 [H]