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The map label for this gene is smaIM [H]

Identifier: 66046061

GI number: 66046061

Start: 3399004

End: 3400125

Strand: Direct

Name: smaIM [H]

Synonym: Psyr_2825

Alternate gene names: 66046061

Gene position: 3399004-3400125 (Clockwise)

Preceding gene: 66046059

Following gene: 66046063

Centisome position: 55.78

GC content: 61.85

Gene sequence:

>1122_bases
ATGAGCCAGCTCCACCAGATACTGGTAGGCGACTGCATCGACATGATGCGCACGCTGCCAGATGAAAGCGTGCATACGTG
CGTGACCAGTCCGCCCTACTACGGTCTGCGTGACTACGGCGTCGAGGGCCAGATCGGCCTGGAGGAAACCCCTGCCGAGT
TCATCGCTCGGTTGGTCGACGTGTTCCGCGAAGTGCGCAGGGTGCTACGCGCTGACGGCACGATCTGGGTGAACATGGGA
GACAGCTACGCCGGCAGCTGGGGCTCCCAAGGCAGGCCCCAAGGCGATGGCCAGATGTCCGGGCGTAGCGTTACGTCTGC
TCGGCAGATCAATGAGCACCCCAGCTTCAAATCCGGTACCGGTGTACGCGGACGCGAGATGAGCATGAAGCCCAAGGATT
TAATGGGCATGCCCTGGCGCCTGGCATTCGCCCTGCAGAACGACGGCTGGTATCTGCGGCAGGACATCATCTGGAACAAA
CCGAACCCGATGCCTGAGAGCGTGCGGGACCGGTGCACCAAGTCGCATGAATACATCTTCCTGCTGAGCAAATCGAAGAA
GTACTACTTCGACCAGACGGCCATTCTCGAACCTTGTTCCCCGAACACCCATGCCCGTCTGTCTCAGGACGTTCAAGCGC
AGATCGGCAGCGAGCGCGCCAACGGCGGAGCTAAGAGCAACGGCAACATGAAGGCGACTACGCGGAAGTCAAACGGTGTG
GGCTGGGGTCACGGCACAGACGCCGACGACCGCCAGCGCGGTAGGGTCAAAGACAACGAGTCAATGAACTCAGCCTTGGC
GATCATGCCAACCGAGCGGAACAAGCGCAGCGTTTGGACTGTGGCCACACACAGCTTCAAGGGCGCCCACTTCGCCACCT
TCCCGCCCGACCTGATCAGGCCCTGCATCCTTGCCGGTGCGCCGCGCGGTGGCGTGGTCCTGGACCCATTCGGCGGTGCC
GGTACCACATCGCTGGTTTCGATGCAGGAAGGACGACGCTCAGTCATCTGCGAGCTGAACCCAGAATATGCGGCACTCGC
CCGCGCCCGGATAGACGCAGCTTGGCTGGATGGCGCTGCTCAGATGGACGTGTTTCGGGACTCGGTACACGGGGCGGGAT
AG

Upstream 100 bases:

>100_bases
ACACGCTCCAGCCTCCTGACGTTGATCTCCTGATTTAAACGTACATCCAGTCAAATTGCCACTATCCGATTCGACTTGTG
GCGCAAACTTGGAATTCGAT

Downstream 100 bases:

>100_bases
CTGATTGTCACCATTTTCCATCACGGTTTCTAACCATTGCGAAAGATTTGAATGCCGGTCTTACTTTATAGCACTCGATC
TTAGATAAGTTGTGGCCCAA

Product: DNA methylase N-4/N-6

Products: NA

Alternate protein names: M.SmaI; N-4 cytosine-specific methyltransferase SmaI [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MSQLHQILVGDCIDMMRTLPDESVHTCVTSPPYYGLRDYGVEGQIGLEETPAEFIARLVDVFREVRRVLRADGTIWVNMG
DSYAGSWGSQGRPQGDGQMSGRSVTSARQINEHPSFKSGTGVRGREMSMKPKDLMGMPWRLAFALQNDGWYLRQDIIWNK
PNPMPESVRDRCTKSHEYIFLLSKSKKYYFDQTAILEPCSPNTHARLSQDVQAQIGSERANGGAKSNGNMKATTRKSNGV
GWGHGTDADDRQRGRVKDNESMNSALAIMPTERNKRSVWTVATHSFKGAHFATFPPDLIRPCILAGAPRGGVVLDPFGGA
GTTSLVSMQEGRRSVICELNPEYAALARARIDAAWLDGAAQMDVFRDSVHGAG

Sequences:

>Translated_373_residues
MSQLHQILVGDCIDMMRTLPDESVHTCVTSPPYYGLRDYGVEGQIGLEETPAEFIARLVDVFREVRRVLRADGTIWVNMG
DSYAGSWGSQGRPQGDGQMSGRSVTSARQINEHPSFKSGTGVRGREMSMKPKDLMGMPWRLAFALQNDGWYLRQDIIWNK
PNPMPESVRDRCTKSHEYIFLLSKSKKYYFDQTAILEPCSPNTHARLSQDVQAQIGSERANGGAKSNGNMKATTRKSNGV
GWGHGTDADDRQRGRVKDNESMNSALAIMPTERNKRSVWTVATHSFKGAHFATFPPDLIRPCILAGAPRGGVVLDPFGGA
GTTSLVSMQEGRRSVICELNPEYAALARARIDAAWLDGAAQMDVFRDSVHGAG
>Mature_372_residues
SQLHQILVGDCIDMMRTLPDESVHTCVTSPPYYGLRDYGVEGQIGLEETPAEFIARLVDVFREVRRVLRADGTIWVNMGD
SYAGSWGSQGRPQGDGQMSGRSVTSARQINEHPSFKSGTGVRGREMSMKPKDLMGMPWRLAFALQNDGWYLRQDIIWNKP
NPMPESVRDRCTKSHEYIFLLSKSKKYYFDQTAILEPCSPNTHARLSQDVQAQIGSERANGGAKSNGNMKATTRKSNGVG
WGHGTDADDRQRGRVKDNESMNSALAIMPTERNKRSVWTVATHSFKGAHFATFPPDLIRPCILAGAPRGGVVLDPFGGAG
TTSLVSMQEGRRSVICELNPEYAALARARIDAAWLDGAAQMDVFRDSVHGAG

Specific function: This methylase recognizes the double-stranded sequence CCCGGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the SmaI endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR017985
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.113 [H]

Molecular weight: Translated: 41111; Mature: 40980

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQLHQILVGDCIDMMRTLPDESVHTCVTSPPYYGLRDYGVEGQIGLEETPAEFIARLVD
CCHHHHHHHHHHHHHHHHCCCCHHHEEECCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHH
VFREVRRVLRADGTIWVNMGDSYAGSWGSQGRPQGDGQMSGRSVTSARQINEHPSFKSGT
HHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCC
GVRGREMSMKPKDLMGMPWRLAFALQNDGWYLRQDIIWNKPNPMPESVRDRCTKSHEYIF
CCCCCCCCCCCHHHCCCCEEEEEEEECCCEEEEECEEECCCCCCCHHHHHHHCCCCCEEE
LLSKSKKYYFDQTAILEPCSPNTHARLSQDVQAQIGSERANGGAKSNGNMKATTRKSNGV
EEECCCCEEECCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCC
GWGHGTDADDRQRGRVKDNESMNSALAIMPTERNKRSVWTVATHSFKGAHFATFPPDLIR
CCCCCCCCCCHHCCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCEECCCCHHHHH
PCILAGAPRGGVVLDPFGGAGTTSLVSMQEGRRSVICELNPEYAALARARIDAAWLDGAA
HHHHCCCCCCCEEECCCCCCCCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCHH
QMDVFRDSVHGAG
HHHHHHHHHCCCC
>Mature Secondary Structure 
SQLHQILVGDCIDMMRTLPDESVHTCVTSPPYYGLRDYGVEGQIGLEETPAEFIARLVD
CHHHHHHHHHHHHHHHHCCCCHHHEEECCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHH
VFREVRRVLRADGTIWVNMGDSYAGSWGSQGRPQGDGQMSGRSVTSARQINEHPSFKSGT
HHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCC
GVRGREMSMKPKDLMGMPWRLAFALQNDGWYLRQDIIWNKPNPMPESVRDRCTKSHEYIF
CCCCCCCCCCCHHHCCCCEEEEEEEECCCEEEEECEEECCCCCCCHHHHHHHCCCCCEEE
LLSKSKKYYFDQTAILEPCSPNTHARLSQDVQAQIGSERANGGAKSNGNMKATTRKSNGV
EEECCCCEEECCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCC
GWGHGTDADDRQRGRVKDNESMNSALAIMPTERNKRSVWTVATHSFKGAHFATFPPDLIR
CCCCCCCCCCHHCCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCEECCCCHHHHH
PCILAGAPRGGVVLDPFGGAGTTSLVSMQEGRRSVICELNPEYAALARARIDAAWLDGAA
HHHHCCCCCCCEEECCCCCCCCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCHH
QMDVFRDSVHGAG
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2690008; 2251121 [H]