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The map label for this gene is oprM [H]

Identifier: 66045721

GI number: 66045721

Start: 2886987

End: 2888450

Strand: Direct

Name: oprM [H]

Synonym: Psyr_2485

Alternate gene names: 66045721

Gene position: 2886987-2888450 (Clockwise)

Preceding gene: 66045720

Following gene: 66045722

Centisome position: 47.38

GC content: 62.09

Gene sequence:

>1464_bases
ATGACCGAACGCCTGGCATTCACCTCCCGCCCGACAGGGCTGATTCGTCCATTCGCGCTGGGGCTTTGTGTGGTGCTGCT
CAGCGCCTGCGCTGTGGGTCCGGATTACCAGAAGCCGTCCATGGCCGCACCCGCGCAGTTCAAGGCTGCGCAGGGCTGGC
GTACGGCGACGCCGAGTGACGGGATGGGCAAAGGTGCGTGGTGGGAGGTGTACAAGGACCCGCAACTGAACGCGCTGGTC
GAGCGCCTTAACACCTCCAACCAGACCGTCGCCCAGTACGATGCGCAGTATCGACAGGCACAGACACTGGTGCGCAGCTC
CAGGGCTGCATTCCTGCCTACGCTTGACCTGACAACCGGCAAAACCCGCTCGGGGCAGGGCACAGGCACGTCGAATAGCG
GCACGAGTGCCTCGGGGATTCGCAACAGTTACAACGCGCAACTGGGCGTCAGTTGGGAGGCCGATGTCTGGGGCAAGCTG
CGTCGCGGGCTGGAGGCTGACACCGCCAATGCTCAGGCAAGTCTTGCGGACCTGGCCGCGATGCGTCTGAGTCTGCAATC
GGAGCTGGTACAGAATTACCTGCAATTGCGCGTGATCGATGAACAAAAACGCCTGCTCGAATCCACAGTGGATGCCTATC
AGCGCTCCCTGACGCTGACGCAGAACCAGTACCGGGCCGGTATCTCCGGAAGTGATGCTGTCGCGCAGGCGCAGACGCAG
TTGAAAAGCACCCAGGCCGATCTGATCGATCTGGCCTGGCAGCGTGCGCAATTCGAGAATGCCATCGCGGTACTGATGGG
CATGGCACCGGCAGACTTCAATCTGCCCGCCAGCACCTCGATTCCGCAGTTGCCGCAGATCCCGCCAGGCCTGCCTTCGC
AGCTGCTCGAACGCCGACCTGACATTGCCGCAGCCGAGCGCTCGGTGATGGGCGCCAATGCCAACATCGGCGTCGCCAGG
GCCGCTTACTACCCGGACTTCACGCTGAGCATGAGCGGCGGATACAGTAGCAGTACCTTTGCCAACTGGATCAGCCTGCC
CAACCGCTTCTGGTCGGTAGGCCCGCAGCTGGCGCTGACCCTGTTCGACGGCGGCAGGCGCTCGGCCGAGGTTGATCGTA
CCGAGGCCGTGTATGACCAGACTGTTGCCCAGTACCGGCAGACCGTGCTGAACGGTTTTCAGGAGGTCGAGAACTATCTG
GTTCAGCTCAAGGTATACGAGCAGGAGGCTGCGGTGCGCCAGGAAGCGCTGGTCTCGGCTCGTGATTCGCTGCGCCTGAC
CAACAATCAATACAAGGCCGGGCTGATCGGTTATCTGGACGTCGTCAATGTTCAGGCGACTGCCTTGAGCAACGAACGCA
GTGTGCTCAGCGTGCTGCAAAGCCGGTTGATTGCCAGCGTTCAATTGATCGCCGCACTGGGCGGGGGCTGGGATGCCGAA
ACAGGTTTGACGCTCCGGGAATAA

Upstream 100 bases:

>100_bases
TTTACACCACTCCGGTGGTTTACCTTTATCTCGACCGTCTGCGCCATCGTTACAACAAATGGCGTGGCGTGCGCACCGAT
GCTGCTCTGGAAACTCCGCT

Downstream 100 bases:

>100_bases
GTATTTCGGGTTCAGAACGCAGATTTTCAGTGGAACTGTTCGCAGTAAACGAAAAAATAATAGCTATTCGCCAGCTTTGC
GACCAAGTGCCGCGACATTC

Product: RND efflux system, outer membrane lipoprotein, NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MTERLAFTSRPTGLIRPFALGLCVVLLSACAVGPDYQKPSMAAPAQFKAAQGWRTATPSDGMGKGAWWEVYKDPQLNALV
ERLNTSNQTVAQYDAQYRQAQTLVRSSRAAFLPTLDLTTGKTRSGQGTGTSNSGTSASGIRNSYNAQLGVSWEADVWGKL
RRGLEADTANAQASLADLAAMRLSLQSELVQNYLQLRVIDEQKRLLESTVDAYQRSLTLTQNQYRAGISGSDAVAQAQTQ
LKSTQADLIDLAWQRAQFENAIAVLMGMAPADFNLPASTSIPQLPQIPPGLPSQLLERRPDIAAAERSVMGANANIGVAR
AAYYPDFTLSMSGGYSSSTFANWISLPNRFWSVGPQLALTLFDGGRRSAEVDRTEAVYDQTVAQYRQTVLNGFQEVENYL
VQLKVYEQEAAVRQEALVSARDSLRLTNNQYKAGLIGYLDVVNVQATALSNERSVLSVLQSRLIASVQLIAALGGGWDAE
TGLTLRE

Sequences:

>Translated_487_residues
MTERLAFTSRPTGLIRPFALGLCVVLLSACAVGPDYQKPSMAAPAQFKAAQGWRTATPSDGMGKGAWWEVYKDPQLNALV
ERLNTSNQTVAQYDAQYRQAQTLVRSSRAAFLPTLDLTTGKTRSGQGTGTSNSGTSASGIRNSYNAQLGVSWEADVWGKL
RRGLEADTANAQASLADLAAMRLSLQSELVQNYLQLRVIDEQKRLLESTVDAYQRSLTLTQNQYRAGISGSDAVAQAQTQ
LKSTQADLIDLAWQRAQFENAIAVLMGMAPADFNLPASTSIPQLPQIPPGLPSQLLERRPDIAAAERSVMGANANIGVAR
AAYYPDFTLSMSGGYSSSTFANWISLPNRFWSVGPQLALTLFDGGRRSAEVDRTEAVYDQTVAQYRQTVLNGFQEVENYL
VQLKVYEQEAAVRQEALVSARDSLRLTNNQYKAGLIGYLDVVNVQATALSNERSVLSVLQSRLIASVQLIAALGGGWDAE
TGLTLRE
>Mature_486_residues
TERLAFTSRPTGLIRPFALGLCVVLLSACAVGPDYQKPSMAAPAQFKAAQGWRTATPSDGMGKGAWWEVYKDPQLNALVE
RLNTSNQTVAQYDAQYRQAQTLVRSSRAAFLPTLDLTTGKTRSGQGTGTSNSGTSASGIRNSYNAQLGVSWEADVWGKLR
RGLEADTANAQASLADLAAMRLSLQSELVQNYLQLRVIDEQKRLLESTVDAYQRSLTLTQNQYRAGISGSDAVAQAQTQL
KSTQADLIDLAWQRAQFENAIAVLMGMAPADFNLPASTSIPQLPQIPPGLPSQLLERRPDIAAAERSVMGANANIGVARA
AYYPDFTLSMSGGYSSSTFANWISLPNRFWSVGPQLALTLFDGGRRSAEVDRTEAVYDQTVAQYRQTVLNGFQEVENYLV
QLKVYEQEAAVRQEALVSARDSLRLTNNQYKAGLIGYLDVVNVQATALSNERSVLSVLQSRLIASVQLIAALGGGWDAET
GLTLRE

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=463, Percent_Identity=28.0777537796976, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI2367348, Length=475, Percent_Identity=27.3684210526316, Blast_Score=149, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 52890; Mature: 52759

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERLAFTSRPTGLIRPFALGLCVVLLSACAVGPDYQKPSMAAPAQFKAAQGWRTATPSD
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCCCC
GMGKGAWWEVYKDPQLNALVERLNTSNQTVAQYDAQYRQAQTLVRSSRAAFLPTLDLTTG
CCCCCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHCCEEEECCCC
KTRSGQGTGTSNSGTSASGIRNSYNAQLGVSWEADVWGKLRRGLEADTANAQASLADLAA
CCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHH
MRLSLQSELVQNYLQLRVIDEQKRLLESTVDAYQRSLTLTQNQYRAGISGSDAVAQAQTQ
HHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCHHHHHHHHH
LKSTQADLIDLAWQRAQFENAIAVLMGMAPADFNLPASTSIPQLPQIPPGLPSQLLERRP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCC
DIAAAERSVMGANANIGVARAAYYPDFTLSMSGGYSSSTFANWISLPNRFWSVGPQLALT
CHHHHHHHHCCCCCCCCEEEEEECCCEEEEECCCCCCHHHHHHHHCCHHHHHCCCEEEEE
LFDGGRRSAEVDRTEAVYDQTVAQYRQTVLNGFQEVENYLVQLKVYEQEAAVRQEALVSA
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHH
RDSLRLTNNQYKAGLIGYLDVVNVQATALSNERSVLSVLQSRLIASVQLIAALGGGWDAE
HHHHEECCCCCCCCHHHHHHHHEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TGLTLRE
CCCEECC
>Mature Secondary Structure 
TERLAFTSRPTGLIRPFALGLCVVLLSACAVGPDYQKPSMAAPAQFKAAQGWRTATPSD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCCCC
GMGKGAWWEVYKDPQLNALVERLNTSNQTVAQYDAQYRQAQTLVRSSRAAFLPTLDLTTG
CCCCCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHCCEEEECCCC
KTRSGQGTGTSNSGTSASGIRNSYNAQLGVSWEADVWGKLRRGLEADTANAQASLADLAA
CCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHH
MRLSLQSELVQNYLQLRVIDEQKRLLESTVDAYQRSLTLTQNQYRAGISGSDAVAQAQTQ
HHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCHHHHHHHHH
LKSTQADLIDLAWQRAQFENAIAVLMGMAPADFNLPASTSIPQLPQIPPGLPSQLLERRP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCC
DIAAAERSVMGANANIGVARAAYYPDFTLSMSGGYSSSTFANWISLPNRFWSVGPQLALT
CHHHHHHHHCCCCCCCCEEEEEECCCEEEEECCCCCCHHHHHHHHCCHHHHHCCCEEEEE
LFDGGRRSAEVDRTEAVYDQTVAQYRQTVLNGFQEVENYLVQLKVYEQEAAVRQEALVSA
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHH
RDSLRLTNNQYKAGLIGYLDVVNVQATALSNERSVLSVLQSRLIASVQLIAALGGGWDAE
HHHHEECCCCCCCCHHHHHHHHEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TGLTLRE
CCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]