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The map label for this gene is potH [H]

Identifier: 66045636

GI number: 66045636

Start: 2782168

End: 2783124

Strand: Reverse

Name: potH [H]

Synonym: Psyr_2400

Alternate gene names: 66045636

Gene position: 2783124-2782168 (Counterclockwise)

Preceding gene: 66045637

Following gene: 66045635

Centisome position: 45.67

GC content: 61.76

Gene sequence:

>957_bases
ATGGCTGGTCAAGTAAAGATGACAGCGGTTGCTCCTCGTCAATCCATGCCGGCCGGGGGTGCTTCCAGCACCGCTGGTGC
GGCCAGCCAGAAGGCGCCGGGCATGCCGCCATCCCCTTCCCTCGCACAGCGCTGGCGTGGCAGCCGCAATCTGCTTCCGG
CGCTGATCTTCCTCGGACTGTTCTTCTTCGCGCCGCTGATTGCTCTACTGCTGCGCGGCGTGCTTGAGCCGGTGCCGGGC
CTTGGCAACTATGAGCAACTGTTTGCCAACTCGGCCTACTCGCGGGTGCTGCTCAATACCTTTTCCGTAGCCGGTCTGGT
GACGCTGTTCAGCCTGCTGCTGGGCTTTCCGCTCGCCTGGGTGATCACCCTGGTGCCGCGCGGCTGGGGCCGCTGGATTC
TCAATATCGTGCTGCTGTCGATGTGGACCAGCCTGCTGGCCCGCACCTATTCGTGGCTGGTGCTGTTGCAGGCCTCCGGG
GTCGTCAACAAGGCCTTGATGGCCATGGGTATCACCGATCAACCGCTGGAAATGGTGCACAACCTGACCGGTGTGGTGAT
CGGCATGAGCTACATCATGATTCCGTTCATCGTCCTGCCATTGCAGGCGACCATGCAGGCCATCGATCCCATGGTGCTGC
AGGCGGGCTCGATCTGCGGTGCCAGCCCTTGGAGCAATTTCTTCAGAGTGTTTCTGCCACTGTGCCGTCCTGGGCTGTTT
TCCGGAGGCCTGATGGTGTTTGTCATGTCACTCGGTTACTACGTCACCCCGGCACTGCTCGGCGGCGCGCAGAACATGAT
GCTGCCCGAATTCATCGTCCAGCAGGTGCAGTCGTTCCTCAACTGGGGGCTGGCCAGTGCCGCCGCTGCGTTGTTGGTGC
TGATCACGCTGGTGCTGTTCTACTTCTACCTGAAGCTCCAGCCGGAATCACCGGTCGGCGCCAGCAACGCGAGGTAA

Upstream 100 bases:

>100_bases
TGGCACCTAACCTGCCGACCGCGCACGTTGCCGATCAAATCACTCTCGACTTCGCATACTGGGCCAAGAACGGCGCTGAT
ATTGCGACACGGTGGAACGA

Downstream 100 bases:

>100_bases
ACCGCCATGCTCCTGACACCCAATGCCATGAGCCCCGGCTTGCGTACGGGCCTGTACCTGACCACCGCCCTGATCGCGCT
GTTCCTGTTGCTGCCGATCC

Product: binding-protein dependent transport system inner membrane protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MAGQVKMTAVAPRQSMPAGGASSTAGAASQKAPGMPPSPSLAQRWRGSRNLLPALIFLGLFFFAPLIALLLRGVLEPVPG
LGNYEQLFANSAYSRVLLNTFSVAGLVTLFSLLLGFPLAWVITLVPRGWGRWILNIVLLSMWTSLLARTYSWLVLLQASG
VVNKALMAMGITDQPLEMVHNLTGVVIGMSYIMIPFIVLPLQATMQAIDPMVLQAGSICGASPWSNFFRVFLPLCRPGLF
SGGLMVFVMSLGYYVTPALLGGAQNMMLPEFIVQQVQSFLNWGLASAAAALLVLITLVLFYFYLKLQPESPVGASNAR

Sequences:

>Translated_318_residues
MAGQVKMTAVAPRQSMPAGGASSTAGAASQKAPGMPPSPSLAQRWRGSRNLLPALIFLGLFFFAPLIALLLRGVLEPVPG
LGNYEQLFANSAYSRVLLNTFSVAGLVTLFSLLLGFPLAWVITLVPRGWGRWILNIVLLSMWTSLLARTYSWLVLLQASG
VVNKALMAMGITDQPLEMVHNLTGVVIGMSYIMIPFIVLPLQATMQAIDPMVLQAGSICGASPWSNFFRVFLPLCRPGLF
SGGLMVFVMSLGYYVTPALLGGAQNMMLPEFIVQQVQSFLNWGLASAAAALLVLITLVLFYFYLKLQPESPVGASNAR
>Mature_317_residues
AGQVKMTAVAPRQSMPAGGASSTAGAASQKAPGMPPSPSLAQRWRGSRNLLPALIFLGLFFFAPLIALLLRGVLEPVPGL
GNYEQLFANSAYSRVLLNTFSVAGLVTLFSLLLGFPLAWVITLVPRGWGRWILNIVLLSMWTSLLARTYSWLVLLQASGV
VNKALMAMGITDQPLEMVHNLTGVVIGMSYIMIPFIVLPLQATMQAIDPMVLQAGSICGASPWSNFFRVFLPLCRPGLFS
GGLMVFVMSLGYYVTPALLGGAQNMMLPEFIVQQVQSFLNWGLASAAAALLVLITLVLFYFYLKLQPESPVGASNAR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=231, Percent_Identity=35.0649350649351, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1787369, Length=215, Percent_Identity=31.1627906976744, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1787713, Length=229, Percent_Identity=31.0043668122271, Blast_Score=96, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34241; Mature: 34110

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGQVKMTAVAPRQSMPAGGASSTAGAASQKAPGMPPSPSLAQRWRGSRNLLPALIFLGL
CCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHH
FFFAPLIALLLRGVLEPVPGLGNYEQLFANSAYSRVLLNTFSVAGLVTLFSLLLGFPLAW
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VITLVPRGWGRWILNIVLLSMWTSLLARTYSWLVLLQASGVVNKALMAMGITDQPLEMVH
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHH
NLTGVVIGMSYIMIPFIVLPLQATMQAIDPMVLQAGSICGASPWSNFFRVFLPLCRPGLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCC
SGGLMVFVMSLGYYVTPALLGGAQNMMLPEFIVQQVQSFLNWGLASAAAALLVLITLVLF
CCHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
YFYLKLQPESPVGASNAR
HHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
AGQVKMTAVAPRQSMPAGGASSTAGAASQKAPGMPPSPSLAQRWRGSRNLLPALIFLGL
CCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHH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HHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]