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The map label for this gene is citE [H]

Identifier: 66045411

GI number: 66045411

Start: 2524631

End: 2525446

Strand: Reverse

Name: citE [H]

Synonym: Psyr_2171

Alternate gene names: 66045411

Gene position: 2525446-2524631 (Counterclockwise)

Preceding gene: 66045412

Following gene: 66045410

Centisome position: 41.44

GC content: 57.72

Gene sequence:

>816_bases
ATGAGCAAATCACTTGTTCGCTCCGCCCTGTTTGTGCCGGCCACTCGACCAGAGCGCATCTCAAAAGCTATTGCCACCGG
GGCGGATCGGGTCATCGTTGACTTGGAAGATGCCGTGCAAGAAAACCTCAAGGAGCAGGCGAGGGATAACCTCGACGCAT
TTCTGAGCGAAGCCCCGGATGCACGAATTCTGGTCCGAGTGAATGCCCCGGATCACTGGGCGCATGTTGCGGACCTGGAT
CTTTGCCGACGCCACGCTGGCGTTATCGGAATTCTGCTGCCCAAAGCAGAAAGCGCGAAACAGGTCACCACAGCTGCCGG
TACCGAAAAACCGATTTGGCCAATCATCGAGAGCGCGAAGGGCTTGTCAGCACTCAACGAAATTGCCGGCACTCAAGGCG
TTGAGCGGTTGTCGTTCGGTAGTCTGGATCTGGGTCTGGACCTGAACCTGACTACTGGCAGCCCTGCCGCCGAAGAAATC
CTTAGCCATGCGCGCTACGCCGTTTTGCTCGCCACCCGGGTTGCAGCGCTCGCTCCAGCGTTGGATGGGGTATTTCCGTC
GTTCCAGGACACCGCTGGGCTACTACGCGCGGTTCATTTTGCACGCGACATGGGCTTTGGTGGCGCGCTGTGTATCCATC
CAACCCAAGTCGCAATCATTCATCAGGCGCTAAAACCAAGCCAGGAAGAATTGCAGTGGGCGCAACGCATTATCGAAGCC
GCTCGTTCAGGTGAAGGCGTTTTCGTGCTGGATGGGCAAATGGTCGATGCGCCGGTCATTGGTCGCGCGCAATCCATTTT
GGCTCGCGCGATCTGA

Upstream 100 bases:

>100_bases
GCGGACGTCTAATCGAAAACGGCAAGGCACAGGTCTGGGCTGGCAATCAGGACGGCACTGCACAGATCGGAGAGATCGAG
TTCAGCAATGGAGCAACGCC

Downstream 100 bases:

>100_bases
TTCAAAACCGGCGCTGTTGCTTAGAAGCTATGCATCCATTCCCATCCTGCAGGACGGTGTCATGACTAACGTTTCCAGCC
TTAGACCCGTGGCATCGATC

Product: HpcH/HpaI aldolase

Products: acetate; oxaloacetate

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MSKSLVRSALFVPATRPERISKAIATGADRVIVDLEDAVQENLKEQARDNLDAFLSEAPDARILVRVNAPDHWAHVADLD
LCRRHAGVIGILLPKAESAKQVTTAAGTEKPIWPIIESAKGLSALNEIAGTQGVERLSFGSLDLGLDLNLTTGSPAAEEI
LSHARYAVLLATRVAALAPALDGVFPSFQDTAGLLRAVHFARDMGFGGALCIHPTQVAIIHQALKPSQEELQWAQRIIEA
ARSGEGVFVLDGQMVDAPVIGRAQSILARAI

Sequences:

>Translated_271_residues
MSKSLVRSALFVPATRPERISKAIATGADRVIVDLEDAVQENLKEQARDNLDAFLSEAPDARILVRVNAPDHWAHVADLD
LCRRHAGVIGILLPKAESAKQVTTAAGTEKPIWPIIESAKGLSALNEIAGTQGVERLSFGSLDLGLDLNLTTGSPAAEEI
LSHARYAVLLATRVAALAPALDGVFPSFQDTAGLLRAVHFARDMGFGGALCIHPTQVAIIHQALKPSQEELQWAQRIIEA
ARSGEGVFVLDGQMVDAPVIGRAQSILARAI
>Mature_270_residues
SKSLVRSALFVPATRPERISKAIATGADRVIVDLEDAVQENLKEQARDNLDAFLSEAPDARILVRVNAPDHWAHVADLDL
CRRHAGVIGILLPKAESAKQVTTAAGTEKPIWPIIESAKGLSALNEIAGTQGVERLSFGSLDLGLDLNLTTGSPAAEEIL
SHARYAVLLATRVAALAPALDGVFPSFQDTAGLLRAVHFARDMGFGGALCIHPTQVAIIHQALKPSQEELQWAQRIIEAA
RSGEGVFVLDGQMVDAPVIGRAQSILARAI

Specific function: May play a role in fatty acid biosynthesis (Potential) [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit-like subfamily [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=295, Percent_Identity=28.8135593220339, Blast_Score=108, Evalue=6e-24,
Organism=Escherichia coli, GI87081764, Length=286, Percent_Identity=33.5664335664336, Blast_Score=100, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17557458, Length=296, Percent_Identity=26.3513513513513, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 28878; Mature: 28747

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSLVRSALFVPATRPERISKAIATGADRVIVDLEDAVQENLKEQARDNLDAFLSEAPD
CCHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCC
ARILVRVNAPDHWAHVADLDLCRRHAGVIGILLPKAESAKQVTTAAGTEKPIWPIIESAK
CEEEEEECCCCHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHCCCCCCCHHHHHHHH
GLSALNEIAGTQGVERLSFGSLDLGLDLNLTTGSPAAEEILSHARYAVLLATRVAALAPA
HHHHHHHHHCCCCHHHHCCCCEEECEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LDGVFPSFQDTAGLLRAVHFARDMGFGGALCIHPTQVAIIHQALKPSQEELQWAQRIIEA
HHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHH
ARSGEGVFVLDGQMVDAPVIGRAQSILARAI
HHCCCCEEEECCCEECCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SKSLVRSALFVPATRPERISKAIATGADRVIVDLEDAVQENLKEQARDNLDAFLSEAPD
CHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCC
ARILVRVNAPDHWAHVADLDLCRRHAGVIGILLPKAESAKQVTTAAGTEKPIWPIIESAK
CEEEEEECCCCHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHCCCCCCCHHHHHHHH
GLSALNEIAGTQGVERLSFGSLDLGLDLNLTTGSPAAEEILSHARYAVLLATRVAALAPA
HHHHHHHHHCCCCHHHHCCCCEEECEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LDGVFPSFQDTAGLLRAVHFARDMGFGGALCIHPTQVAIIHQALKPSQEELQWAQRIIEA
HHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHH
ARSGEGVFVLDGQMVDAPVIGRAQSILARAI
HHCCCCEEEECCCEECCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0565 {oxaoacetate}} 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]