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The map label for this gene is nasD [H]

Identifier: 66045340

GI number: 66045340

Start: 2449326

End: 2450555

Strand: Reverse

Name: nasD [H]

Synonym: Psyr_2100

Alternate gene names: 66045340

Gene position: 2450555-2449326 (Counterclockwise)

Preceding gene: 66045341

Following gene: 66045339

Centisome position: 40.21

GC content: 60.89

Gene sequence:

>1230_bases
ATGACCAAACTCAAACTGGTGATGATCGGCAACGGCATGGCCGGCGTGCGCACACTCGAAGAGTTGCTGAAACTGAGCGA
AGACCTTTACCACATCACGGTCTTCGGGGCCGAGCCACACCCCAATTACAATCGCATCCTGCTGTCGCCGGTGCTGGCCG
GCGAACAGAATTTCGAAGACATCGTGCTCAACGATCTCGACTGGTACCAACGCAACGGTATCGAGTTGTTGCTCAACCGC
CGCGTGGTGAAGATCGATCGCATCAAACGCCTGGTGATCGCCGATGACGGCACCCAGGCCCATTATGATCGGCTGCTGAT
TGCCACCGGTTCGCGGCCGTTCATACTGCCGATACCCGGCAACACGCTGAACGGCGTCATCGGTTATCGCGACATCAGCC
ACACGCGGCAGATGATCGACACCGCCGTCACGCACAAACGCGCAGTCGTCATCGGCGGCGGCCTGCTCGGCCTGGAAGCG
GCCAACGGCTTGAGCCTGCGCGGCATGCAAGTCACGGTTATTCATAATGGCCAGACGCTGCTGGAGCGCCAACTGGACAA
GACCAGCGGGCGGATGCTGCAAGCGGCACTGGAAAAGCGCGGCCTGAGCTTTCGCCTCGACGAACAGACCAACGCCCTGC
TCGGCGATGATCAGGGCCGCGTAACCGCGGTGCAATTCAACAACGGCGACAGAATCGATGCCGATCTGGTGGTCATGGCC
GCAGGCATACGGCCCAATACCGAATTGGCCGAGCAAGCCGGCCTGCCCTGTAACCGCGGCATTCTGGTCAATGACACCTT
GCAGACCTACGACCCGCGCATCTACGCGATTGGCGAATGCGTCAGCCACCGCGGCATTGCCTATGGGCTGGTGGCGCCGC
TGTTCGAGCAGGCGCGGGTCTGCGCCAATCACCTTGCGCAACTGGGCTTCGCGCGTTATCCGGGTTCGGTCACCTCGACC
AAGCTTAAAGTCACCGGCATCGACCTGTTTTCCGCCGGGGATTTCCTCGGCGGTGAAGGCACTGAAAGCATCACCCTTTC
CGATCCCATTGGCGGCGTCTACAAGAAGCTGGTGATCAAGAACGACGTACTGGTCGGCGCCTGCCTGTACGGCGACACCG
CCGACGGCAACTGGTACTTGCAGCAGATCCGTGATGGCCAGGGAATCGACGCCATACGCGATCATCTGATGTTCGGCAAA
CCCGCTGCTGCGCAGGCGGCCTGCGCATGA

Upstream 100 bases:

>100_bases
CGGGACCGGACAAAGGCGTCCTCGTCAGGCCACTGACGGGACGCCTTTTTTTGTTTGCGCGAAACGTGTCGAGCACGCGC
TGCAGTGACTCGGAGGCAAG

Downstream 100 bases:

>100_bases
ACAGCCTTGCCCTTCACACCCCTCGGATCACGGCATCGACCTGCTGCTATTGCGGCGTTGGCTGCGGCGTACTGATCGAA
CATGACGGCGAGCATATTCT

Product: FAD-dependent pyridine nucleotide-disulphide oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MTKLKLVMIGNGMAGVRTLEELLKLSEDLYHITVFGAEPHPNYNRILLSPVLAGEQNFEDIVLNDLDWYQRNGIELLLNR
RVVKIDRIKRLVIADDGTQAHYDRLLIATGSRPFILPIPGNTLNGVIGYRDISHTRQMIDTAVTHKRAVVIGGGLLGLEA
ANGLSLRGMQVTVIHNGQTLLERQLDKTSGRMLQAALEKRGLSFRLDEQTNALLGDDQGRVTAVQFNNGDRIDADLVVMA
AGIRPNTELAEQAGLPCNRGILVNDTLQTYDPRIYAIGECVSHRGIAYGLVAPLFEQARVCANHLAQLGFARYPGSVTST
KLKVTGIDLFSAGDFLGGEGTESITLSDPIGGVYKKLVIKNDVLVGACLYGDTADGNWYLQQIRDGQGIDAIRDHLMFGK
PAAAQAACA

Sequences:

>Translated_409_residues
MTKLKLVMIGNGMAGVRTLEELLKLSEDLYHITVFGAEPHPNYNRILLSPVLAGEQNFEDIVLNDLDWYQRNGIELLLNR
RVVKIDRIKRLVIADDGTQAHYDRLLIATGSRPFILPIPGNTLNGVIGYRDISHTRQMIDTAVTHKRAVVIGGGLLGLEA
ANGLSLRGMQVTVIHNGQTLLERQLDKTSGRMLQAALEKRGLSFRLDEQTNALLGDDQGRVTAVQFNNGDRIDADLVVMA
AGIRPNTELAEQAGLPCNRGILVNDTLQTYDPRIYAIGECVSHRGIAYGLVAPLFEQARVCANHLAQLGFARYPGSVTST
KLKVTGIDLFSAGDFLGGEGTESITLSDPIGGVYKKLVIKNDVLVGACLYGDTADGNWYLQQIRDGQGIDAIRDHLMFGK
PAAAQAACA
>Mature_408_residues
TKLKLVMIGNGMAGVRTLEELLKLSEDLYHITVFGAEPHPNYNRILLSPVLAGEQNFEDIVLNDLDWYQRNGIELLLNRR
VVKIDRIKRLVIADDGTQAHYDRLLIATGSRPFILPIPGNTLNGVIGYRDISHTRQMIDTAVTHKRAVVIGGGLLGLEAA
NGLSLRGMQVTVIHNGQTLLERQLDKTSGRMLQAALEKRGLSFRLDEQTNALLGDDQGRVTAVQFNNGDRIDADLVVMAA
GIRPNTELAEQAGLPCNRGILVNDTLQTYDPRIYAIGECVSHRGIAYGLVAPLFEQARVCANHLAQLGFARYPGSVTSTK
LKVTGIDLFSAGDFLGGEGTESITLSDPIGGVYKKLVIKNDVLVGACLYGDTADGNWYLQQIRDGQGIDAIRDHLMFGKP
AAAQAACA

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=281, Percent_Identity=28.1138790035587, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI226437568, Length=281, Percent_Identity=28.1138790035587, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI65787454, Length=281, Percent_Identity=28.1138790035587, Blast_Score=102, Evalue=7e-22,
Organism=Escherichia coli, GI1789765, Length=415, Percent_Identity=35.421686746988, Blast_Score=201, Evalue=6e-53,
Organism=Escherichia coli, GI1789065, Length=305, Percent_Identity=30.8196721311475, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1788892, Length=254, Percent_Identity=26.3779527559055, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17559934, Length=399, Percent_Identity=26.8170426065163, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI281359715, Length=277, Percent_Identity=24.9097472924188, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI281359713, Length=277, Percent_Identity=24.9097472924188, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24639250, Length=277, Percent_Identity=24.9097472924188, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI18543267, Length=277, Percent_Identity=24.9097472924188, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24639252, Length=277, Percent_Identity=24.9097472924188, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24639257, Length=277, Percent_Identity=24.9097472924188, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24585130, Length=332, Percent_Identity=24.3975903614458, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24581020, Length=305, Percent_Identity=26.8852459016393, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI28573993, Length=305, Percent_Identity=26.8852459016393, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI21358499, Length=235, Percent_Identity=30.2127659574468, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 44497; Mature: 44366

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLKLVMIGNGMAGVRTLEELLKLSEDLYHITVFGAEPHPNYNRILLSPVLAGEQNFED
CCEEEEEEEECCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEEEECCCCCCCCH
IVLNDLDWYQRNGIELLLNRRVVKIDRIKRLVIADDGTQAHYDRLLIATGSRPFILPIPG
HEECCCCHHHHCCEEEEECCCEEEEEHEEEEEEECCCCCCCCCEEEEEECCCCEEEECCC
NTLNGVIGYRDISHTRQMIDTAVTHKRAVVIGGGLLGLEAANGLSLRGMQVTVIHNGQTL
CCCCCEEECCHHHHHHHHHHHHHHCCEEEEEECCEEEEECCCCCEECCEEEEEEECCHHH
LERQLDKTSGRMLQAALEKRGLSFRLDEQTNALLGDDQGRVTAVQFNNGDRIDADLVVMA
HHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEECCCCCCEEEEEECCCCCCCCEEEEEE
AGIRPNTELAEQAGLPCNRGILVNDTLQTYDPRIYAIGECVSHRGIAYGLVAPLFEQARV
ECCCCCHHHHHHCCCCCCCCEEEECCHHHCCCEEEEEEHHHHCCCCHHHHHHHHHHHHHH
CANHLAQLGFARYPGSVTSTKLKVTGIDLFSAGDFLGGEGTESITLSDPIGGVYKKLVIK
HHHHHHHCCHHCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCEEEECCCCCHHHHHHHEE
NDVLVGACLYGDTADGNWYLQQIRDGQGIDAIRDHLMFGKPAAAQAACA
CCEEEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCHHCCCC
>Mature Secondary Structure 
TKLKLVMIGNGMAGVRTLEELLKLSEDLYHITVFGAEPHPNYNRILLSPVLAGEQNFED
CEEEEEEEECCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEEEECCCCCCCCH
IVLNDLDWYQRNGIELLLNRRVVKIDRIKRLVIADDGTQAHYDRLLIATGSRPFILPIPG
HEECCCCHHHHCCEEEEECCCEEEEEHEEEEEEECCCCCCCCCEEEEEECCCCEEEECCC
NTLNGVIGYRDISHTRQMIDTAVTHKRAVVIGGGLLGLEAANGLSLRGMQVTVIHNGQTL
CCCCCEEECCHHHHHHHHHHHHHHCCEEEEEECCEEEEECCCCCEECCEEEEEEECCHHH
LERQLDKTSGRMLQAALEKRGLSFRLDEQTNALLGDDQGRVTAVQFNNGDRIDADLVVMA
HHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEECCCCCCEEEEEECCCCCCCCEEEEEE
AGIRPNTELAEQAGLPCNRGILVNDTLQTYDPRIYAIGECVSHRGIAYGLVAPLFEQARV
ECCCCCHHHHHHCCCCCCCCEEEECCHHHCCCEEEEEEHHHHCCCCHHHHHHHHHHHHHH
CANHLAQLGFARYPGSVTSTKLKVTGIDLFSAGDFLGGEGTESITLSDPIGGVYKKLVIK
HHHHHHHCCHHCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCEEEECCCCCHHHHHHHEE
NDVLVGACLYGDTADGNWYLQQIRDGQGIDAIRDHLMFGKPAAAQAACA
CCEEEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]