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The map label for this gene is folE [H]

Identifier: 66045089

GI number: 66045089

Start: 2123976

End: 2124521

Strand: Reverse

Name: folE [H]

Synonym: Psyr_1845

Alternate gene names: 66045089

Gene position: 2124521-2123976 (Counterclockwise)

Preceding gene: 66045090

Following gene: 66045088

Centisome position: 34.86

GC content: 60.07

Gene sequence:

>546_bases
GTGACACTGGAACAGAATTACACCGCCATCCTCGGGCAACTGGGCGAGGACGTCTCGCGCGAAGGTCTGCTGGACACCCC
CAAGCGCGCCGCCAAGGCCATGCAGTATCTGTGTCGCGGTTACACGCAGACCCTGGAAGAAGTGACCAACGGCGCGCTGT
TCAGCTCCGATGCCAGTGAAATGGTGATGGTCAAGGACATCGAGCTGTACTCGCTGTGCGAGCACCATCTGCTGCCGTTC
ATTGGCAAGGCTCATGTGGCCTACATCCCGCAGGGCAAGGTGCTTGGCTTGTCGAAAGTGGCGCGTATCGTCGACATGTA
TGCCCGCCGTCTGCAGATCCAGGAAAACCTCAGCCGCCAGATCGCCGAGGCCATCCAGCAGGTCACCGGCGCGCTGGGCG
TTGCGGTGGTGATCGAAGCCAAGCACATGTGCATGATGATGCGTGGCGTCGAGAAGCAGAACTCGGCCATGATCACCTCG
GTGATGCTGGGCGAATTCCGTGAAAACGCGGCCACCCGCAGTGAGTTTCTCAGCCTGATCAAGTGA

Upstream 100 bases:

>100_bases
CCACTGGAGCGGGATCGGCAAACGGGACACCAGCTCGATTGGTAAAAAATCGGTACAGACCCAAACGTACCGCTCTGAAA
CCTATACGGAATCACCCTCT

Downstream 100 bases:

>100_bases
TCGTTCAGCCTGCGTTCACCTGCCGTGAGGCACGCTGAACGCACGGCTGGGCACGCCACCGCCCGCAAGAGGTATGACCT
TGTTCATGAAAGCGTTAAGA

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I 2; GTP-CH-I 2 [H]

Number of amino acids: Translated: 181; Mature: 180

Protein sequence:

>181_residues
MTLEQNYTAILGQLGEDVSREGLLDTPKRAAKAMQYLCRGYTQTLEEVTNGALFSSDASEMVMVKDIELYSLCEHHLLPF
IGKAHVAYIPQGKVLGLSKVARIVDMYARRLQIQENLSRQIAEAIQQVTGALGVAVVIEAKHMCMMMRGVEKQNSAMITS
VMLGEFRENAATRSEFLSLIK

Sequences:

>Translated_181_residues
MTLEQNYTAILGQLGEDVSREGLLDTPKRAAKAMQYLCRGYTQTLEEVTNGALFSSDASEMVMVKDIELYSLCEHHLLPF
IGKAHVAYIPQGKVLGLSKVARIVDMYARRLQIQENLSRQIAEAIQQVTGALGVAVVIEAKHMCMMMRGVEKQNSAMITS
VMLGEFRENAATRSEFLSLIK
>Mature_180_residues
TLEQNYTAILGQLGEDVSREGLLDTPKRAAKAMQYLCRGYTQTLEEVTNGALFSSDASEMVMVKDIELYSLCEHHLLPFI
GKAHVAYIPQGKVLGLSKVARIVDMYARRLQIQENLSRQIAEAIQQVTGALGVAVVIEAKHMCMMMRGVEKQNSAMITSV
MLGEFRENAATRSEFLSLIK

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=175, Percent_Identity=57.7142857142857, Blast_Score=231, Evalue=4e-61,
Organism=Homo sapiens, GI4503949, Length=175, Percent_Identity=57.7142857142857, Blast_Score=231, Evalue=4e-61,
Organism=Homo sapiens, GI66932970, Length=157, Percent_Identity=50.3184713375796, Blast_Score=174, Evalue=5e-44,
Organism=Homo sapiens, GI66932972, Length=134, Percent_Identity=55.9701492537313, Blast_Score=173, Evalue=9e-44,
Organism=Escherichia coli, GI1788476, Length=185, Percent_Identity=37.8378378378378, Blast_Score=117, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17560486, Length=174, Percent_Identity=59.7701149425287, Blast_Score=229, Evalue=8e-61,
Organism=Saccharomyces cerevisiae, GI6321706, Length=173, Percent_Identity=58.9595375722543, Blast_Score=218, Evalue=4e-58,
Organism=Drosophila melanogaster, GI24656791, Length=179, Percent_Identity=56.4245810055866, Blast_Score=213, Evalue=5e-56,
Organism=Drosophila melanogaster, GI24656796, Length=179, Percent_Identity=56.4245810055866, Blast_Score=213, Evalue=7e-56,
Organism=Drosophila melanogaster, GI24656782, Length=179, Percent_Identity=56.4245810055866, Blast_Score=213, Evalue=8e-56,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 20114; Mature: 19983

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1 ; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
6.1 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLEQNYTAILGQLGEDVSREGLLDTPKRAAKAMQYLCRGYTQTLEEVTNGALFSSDASE
CCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCC
MVMVKDIELYSLCEHHLLPFIGKAHVAYIPQGKVLGLSKVARIVDMYARRLQIQENLSRQ
EEHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHH
IAEAIQQVTGALGVAVVIEAKHMCMMMRGVEKQNSAMITSVMLGEFRENAATRSEFLSLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
K
C
>Mature Secondary Structure 
TLEQNYTAILGQLGEDVSREGLLDTPKRAAKAMQYLCRGYTQTLEEVTNGALFSSDASE
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCC
MVMVKDIELYSLCEHHLLPFIGKAHVAYIPQGKVLGLSKVARIVDMYARRLQIQENLSRQ
EEHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHH
IAEAIQQVTGALGVAVVIEAKHMCMMMRGVEKQNSAMITSVMLGEFRENAATRSEFLSLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]